public bool StartN3Fluorescence(List <double> shutterTimesMs, double gain,
Callback whenMeasurementEnds, out string error)
{
bool result = false;
error = "";
try
{
if (!_cameraConnected)
{
throw new Exception("Camera not connected");
}
Ready = false;
//stop streaming camera
_ptGreyCamera.StopVideo();
if (!_ptGreyCamera.SetBinning(true))
{
throw new Exception("Could not enable binning");
}
if (!_ptGreyCamera.SetGain(gain))
{
throw new Exception("Could not change gain");
}
//increase frame buffer count
if (!_ptGreyCamera.SetStreamBufferCount(50))
{
throw new Exception("Could not set frame buffer count");
}
//sequencer off
if (!_ptGreyCamera.SetSequencerMode(false))
{
throw new Exception("Could not disable sequencer");
}
//sequencer config on
if (!_ptGreyCamera.SetSequencerConfigurationMode(true))
{
throw new Exception("Could not enable sequencer config");
}
//set sequencer
for (int sequenceNumber = 0; sequenceNumber < shutterTimesMs.Count; sequenceNumber++)
{
if (!_ptGreyCamera.SetSingleSequence(sequenceNumber, shutterTimesMs.Count - 1,
shutterTimesMs[sequenceNumber] * 1000))
{
throw new Exception("Could not set sequence");
}
}
//sequencer config off
if (!_ptGreyCamera.SetSequencerConfigurationMode(false))
{
throw new Exception("Could not disable sequencer config");
}
//sequencer on
if (!_ptGreyCamera.SetSequencerMode(true))
{
throw new Exception("Could not enable sequencer");
}
//do this before we change trigger mode
_phosStartTimestamp = _ptGreyCamera.GetImageTimeStamp();
//empty phos image buffer
_phosImages.Clear();
//set phos count
_phosImagesToCollect = (uint)shutterTimesMs.Count;
//start backgroundworker to wait for completion
BWArgument arg;
arg.measurementEnd = whenMeasurementEnds;
arg.waitTime = (int)(5000 + (shutterTimesMs.Sum()));
_phosMeasureCompletionEvent.Reset();
BackgroundWorker bw = new BackgroundWorker();
bw.DoWork += WaitForN3FluorescenceCompletion;
bw.RunWorkerCompleted += N3FluorescenceComplete;
bw.RunWorkerAsync(arg);
//start video
_ptGreyCamera.StartVideo();
result = true;
}
catch (Exception ex)
{
error = ex.Message;
Ready = true;
_ptGreyCamera.StopVideo();
_ptGreyCamera.SetSequencerConfigurationMode(false);
_ptGreyCamera.SetSequencerMode(false);
_ptGreyCamera.SetBinning(false);
_phosImagesToCollect = 0;
_phosMeasureCompletionEvent.Set();
SetShutterTime(Properties.Settings.Default.ShutterDefault);
SetGain(Properties.Settings.Default.GainDefault);
_ptGreyCamera.StartVideo();
}
return(result);
}
