protected override void OnShow()
{
writer.enabled = true;
writer.ResetToBeginning();
if (dataList.Count > 0)
{
string text = dataList[index].SpeakList[dataIndex];
if (text.Length > 60)
{
addText = text.Substring(60, text.Length - 60);
text = text.Substring(0, 60);
}
nameLabel.text = dataList[index].name;
speakLabel.text = text;
}
else if (data != null)
{
string[] sArray = data.SpeakList[data.index].Split(':');
string[] sArray2 = sArray[1].Split('*');
string text = sArray2[0];
if (text.Length > 60)
{
addText = text.Substring(60, text.Length - 60);
text = text.Substring(0, 60);
}
nameLabel.text = sArray2[1];
//m_Sprite.spriteName = sArray[0];
speakLabel.text = text;
}
IEnumeratorManager.Instance.StartCoroutine(SetAcitveSpeakLabel_Delay());
}
private void ShowTextAfterTp()
{
writer.enabled = true;
canUseE = true;
if (data.cout > 0)
{
//if (dataList[0].spriteName != eventSpriteName)
//{
// eventSpriteName = dataList[0].spriteName;
//}
string text = data.SpeakList[data.index];
if (text.Contains("*"))
{
string[] sArrary = text.Split('*');
text = sArrary[0];
}
if (text.Length > 60)
{
addText = text.Substring(60, text.Length - 61);
text = text.Substring(0, 60);
}
//nameLabel.text = dataList[index].name;
speakLabel.text = text;
}
//else if (data != null)
//{
// string text = data.SpeakList[data.index];
// if (text.Length > 40)
// {
// addText = text.Substring(40, text.Length - 41);
// text = text.Substring(0, 40);
// }
// //nameLabel.text = data.name;
// speakLabel.text = text;
//}
if (bg_GameObject.activeSelf == false)
{
bg_GameObject.SetActive(true);
TweenAlpha ta = bg_GameObject.GetComponent <TweenAlpha>();
ta.enabled = true;
ta.ResetToBeginning();
}
writer.ResetToBeginning();
IEnumeratorManager.Instance.StartCoroutine(SetAcitveSpeakLabel_Delay());
curData = data;
}
protected override void OnShow()
{
AudioManager.Instance.PlayEffect_Source("waterAudio");
AudioClip audioClip = ResourcesManager.Instance.LoadAudioClip(effectAudioName);
if (audioClip != null)
{
typeWriter.printOneCharHader = PlayEffectAduio;
}
else
{
typeWriter.printOneCharHader = null;
}
typeWriter.gameObject.SetActive(false);
typeWriter.enabled = false;
typeWriter.ResetToBeginning();
UILabel label = typeWriter.GetComponent <UILabel>();
if (positionEnum == SubtitlePositionEnum.bottom)
{
label.transform.localPosition = new Vector3(label.transform.localPosition.x, -375, label.transform.localPosition.z);
}
else if (positionEnum == SubtitlePositionEnum.top)
{
label.transform.localPosition = new Vector3(label.transform.localPosition.x, 409.78f, label.transform.localPosition.z);
}
label.text = data.SpeakList[data.Index];
typeWriter.charsPerSecond = perChar;
typeWriter.enabled = true;
typeWriter.gameObject.SetActive(true);
handerIsActive = false;
}
// Update is called once per frame
void Update()
{
if (Input.GetKeyDown(KeyCode.A))
{
label.text = "目的 [n]探索激光显微技术(laser capture microdissection system, LCM)捕获口腔上皮细胞，" +
"并进行STR-DNA分型检测的方法。方法 用VERITAS显微切割仪红外低能激光显微捕获一定数量口腔上皮细胞，" +
"进行Profiler Plus试剂盒STR复合扩增，检测DNA基因型。" +
"结果 7~8个口腔上皮细胞能成功获得STR-DNA分型。3~4个口腔上皮细胞不能成功获得STR-DNA分型。" +
"结论 激光显微捕获作为一种分离单个细胞的新技术，对于微量口腔上皮细胞的STR-DNA分型是可行的。特异性高\n" +
@"首次报导的PCR所用的DNA聚合酶是大肠杆菌的DNAPolymerase I的Klenow大片段，其酶活性在90℃会变性失活，需每次PCR循环都要重新加入Klenow大片段，同时引物是在37℃延伸（聚合）易产生模板—引物之间的碱基错配、致特异性较差，1988年Saiki等从温泉水中分离到的水生嗜热杆菌中提取的热稳定的Taq DNA聚合酶，在热变性处理时不被灭活，不必在每次循环扩增中再加入新酶，可以在较高温度下连续反应，显著地提高PCR产物的特异性，序列分析证明其扩增的DNA序列与原模板DNA一致。
扩增过程中，单核苷酸的错误参入程度很低、其错配率一般只有约万分之一，足可以提供特异性分析，选用各型病毒相对的特异寡核苷酸引物。PCR能一次确定病毒的多重感染。如用HPV11和HPV16型病毒引物检测病妇宫颈刮片细胞可以发现部分病人存在HPV11和HPV16两型的双重感染。
高度敏感
理论上PCR可以按2n倍数扩增DNA十亿倍以上，实际应用已证实可以将极微量的靶DNA成百万倍以上地扩增到足够检测分析量的DNA。能从100万个细胞中检出一个靶细胞，或对诸如病人口液等只含一个感染细胞的标本或仅含0.01pg的感染细胞的特异性片段样品中均可检测。
无放射性
一般在2小时内约可完成30次以上的循环扩增，加上用电泳分析。只需3 - 4小时便可完成，不用分离提纯病毒，DNA粗制品及总RNA均可作为反应起始物，可直接用临床标本如血液、体液、尿液、洗液、脱落毛发、细胞、活体组织等粗制的DNA的提取液来扩增检测，省去费时繁杂的提纯程序，扩增产物用一般电泳分析即可，不一定用同位素，无放射性易于推广。
简便
扩增产物可直接供作序列分析和分子克隆，摆脱繁琐的基因方法，可直接从RNA或染色体DNA中或部分DNA已降解的样品中分离目的基因，省去常规方法中须先进行克隆后再作序列分析的冗繁程序。已固定的和包埋的组织或切片亦可检测。如在PCR引物端事先构建一个内切酶位点，扩增的靶DNA可直接克隆到M13，PUC19等相应酶切位点的载体中。
可扩增物质
先按通常方法用寡脱氧胸苷引物和逆转录酶将mRNA转变成单链cDNA，再将得到的单链cDNA进行PCR扩增，即使mRNA转录片段只有100ngcDNA中的0.01 %，也能经PCR扩增1ng有242碱基对长度的特异片段，有些外显子分散在一段很长的DNA中，难以将整段DNA大分子扩增和做序列分析。若以mRNA作模板，则可将外显子集中，用PCR一次便完成对外显子的扩增并进行序列分析。";
typewriter.ResetToBeginning();
}
if (Input.GetKeyDown(KeyCode.B))
{
//scrollView.UpdatePosition();
//scrollView.OnPan(new Vector2(0f, 1000f));
}
if (label.height != lastHeight)
{
scrollView.ResetPosition();
scrollView.SetDragAmount(0f, 1f, false);
lastHeight = label.height;
}
}
public void Play()
{
Debug.Log("talk In");
if (typewritter.isActive)
{
typewritter.Finish();
Debug.Log("talk");
}
else
{
_idx++;
if (_idx < dic_sample.Count)
{
lb_typer.text = dic_sample[_idx].arg;
typewritter.ResetToBeginning();
setSprite();
}
else
{
colTalk.enabled = false;
spriteClear();
DissMiss();
}
}
}
public void NextSpeech()
{
if (CommuCurrentSpeech < targetCustomer.CommuMax)
{
if (EndMessage)
{
CommuCurrentSpeech++;
UISetting();
NowFirstTalking = true;
typeEffect.ResetToBeginning();
StartCoroutine("BossPopUpSprite");
EndMessage = false;
targetCustomer.CommuCurrentIndex++;
}
else
{
typeEffect.Finish();
EndMessage = true;
}
}
else
{
if (EndMessage)
{
CustomerSpeech.SpeechStart(true);
CustomerSpeech.BossItemGiv(targetCustomer);
CustomerSpeech.BossItemGiv(targetCustomer);
StopTime.StartCoroutine("TimeFlow");
TimeManager.nowFlow = true;
UIVisible(false);
CommuCurrentSpeech = 0;
NowFirstTalking = false;
EndMessage = false;
}
else
{
typeEffect.Finish();
EndMessage = true;
}
}
}
public void ShowChatInfo(string info)
{
if (info != null)
{
mChatInfo.text = info;
SetVisible(true);
mTypewriterEffect.ResetToBeginning();
mTypewriterEffect.enabled = true;
mTweener.Restart();
}
}
public void show(DavinciController.ActionCallback callback)
{
//labelTurn.text = nickName + "[99ff00] TURN [-]";
isNext = true;
if (effect == null)
{
effect = labelTurn.gameObject.GetComponent <TypewriterEffect>();
}
effect.ResetToBeginning();
this.callback = callback;
this.gameObject.SetActive(true);
}
static public int ResetToBeginning(IntPtr l)
{
try {
TypewriterEffect self = (TypewriterEffect)checkSelf(l);
self.ResetToBeginning();
pushValue(l, true);
return(1);
}
catch (Exception e) {
return(error(l, e));
}
}
static int ResetToBeginning(IntPtr L)
{
try
{
ToLua.CheckArgsCount(L, 1);
TypewriterEffect obj = (TypewriterEffect)ToLua.CheckObject(L, 1, typeof(TypewriterEffect));
obj.ResetToBeginning();
return(0);
}
catch (Exception e)
{
return(LuaDLL.toluaL_exception(L, e));
}
}
void StartTypewriting(UILabel label)
{
if (writingLabel != null)
{
writingEffect.Finish();
}
lastHeight = 0f;
writingLabel = label;
writingScrollView = writingLabel.transform.parent.GetComponent <UIScrollView>();
writingEffect = writingLabel.GetComponent <TypewriterEffect>();
writingEffect.ResetToBeginning();
btnMask.SetActive(true);
EventDelegate.Add(writingEffect.onFinished, OnEndTypewriting, true);
}
// 根据当前索引来更新显示文本(直接打印机显示文字效果)
void UpdateText(int text_idx)
{
if (text_idx >= talkContentsList.Count)
{
return;
}
int lang_id = talkContentsList[text_idx];
// string str = Lang.STR(lang_id);
string str = "test";
descLab.text = str;
descTypeEffect.ResetToBeginning();
}
IEnumerator EndAnim()
{
yield return(null);
switch (ClickCount)
{
case 1:
Type1.enabled = true;
firstSlide = false;
break;
case 2:
Ending1.SetTrigger("StartCartoon1");
Type2.enabled = true;
break;
case 3:
Ending2.SetTrigger("StartCartoon2");
Type3.enabled = true;
break;
case 4:
Type4.enabled = true;
break;
case 5:
Text5.text = "[앞으로는 얼굴 볼일 없게 합시다잉]";
Type5.enabled = true;
Type5.ResetToBeginning();
break;
case 7:
Text6.text = "라임은 빚을 모두 갚았습니다!...fin";
Type6.enabled = true;
Type6.ResetToBeginning();
Ending3.SetTrigger("StartCartoon3");
break;
case 8:
takedNum = 0;
StartCoroutine("EndScreenFadeOut");
break;
}
}
private IEnumerator DoAddMessage(string name, string msg, StoryDirector.POS tail_dir, StoryDirector.MSG_TYPE msg_type)
{
typewriter = null;
Transform table_t = GetCtrl(UI.TBL_MESSAGE);
string prefab_name = "StoryMessageItem0";
if (msg_type == StoryDirector.MSG_TYPE.MONOLOGUE)
{
prefab_name = "StoryMessageItem1";
}
Transform message_item_t = Realizes(prefab_name, table_t, true);
message_item_t.SetSiblingIndex(0);
UIWidget message_item_w = message_item_t.GetComponent <UIWidget>();
lastMessageItem = message_item_t;
balloon = base.GetComponent <UISprite>(message_item_t, (Enum)UI.SPR_BALLOON);
tailLeft = FindCtrl(message_item_t, UI.SPR_TAIL_L);
tailRight = FindCtrl(message_item_t, UI.SPR_TAIL_R);
tailCenter = FindCtrl(message_item_t, UI.SPR_TAIL_C);
nameLabel = base.GetComponent <UILabel>(message_item_t, (Enum)UI.LBL_NAME);
messageLabel = base.GetComponent <UILabel>(message_item_t, (Enum)UI.LBL_MESSAGE);
initBaseHeight = message_item_w.height;
messageLabel.text = " ";
initMessageHeight = messageLabel.height;
messageHeight = initMessageHeight;
string temp = string.Empty;
if (messageLabel.Wrap(msg, out temp))
{
msg = WordWrap.Convert(messageLabel, msg);
}
SetLastMessageFocus(true);
SetMessageDragEnabled(false);
if (msg_type == StoryDirector.MSG_TYPE.NORMAL)
{
if (tailLeft != null && tail_dir != StoryDirector.POS.LEFT)
{
tailLeft.get_gameObject().SetActive(false);
}
if (tailRight != null && tail_dir != StoryDirector.POS.RIGHT)
{
tailRight.get_gameObject().SetActive(false);
}
if (tailCenter != null && tail_dir != StoryDirector.POS.CENTER)
{
tailCenter.get_gameObject().SetActive(false);
}
nameLabel.text = name;
}
UIWidget next_arrow_w = base.GetComponent <UIWidget>((Enum)UI.SPR_NEXT);
next_arrow_w.get_gameObject().SetActive(false);
List <UITweener> tweens = new List <UITweener>();
message_item_t.GetComponentsInChildren <UITweener>(tweens);
while (tweens.Find((UITweener o) => o.get_enabled()) != null)
{
yield return((object)null);
}
SoundManager.PlaySystemSE(SoundID.UISE.POPUP, 1f);
messageLabel.text = msg;
typewriter = messageLabel.get_gameObject().AddComponent <TypewriterEffect>();
typewriter.charsPerSecond = StoryDirector.SPEED_TYPEWRITER;
typewriter.ResetToBeginning();
while (typewriter.isActive)
{
yield return((object)null);
}
yield return((object)null);
next_arrow_w.get_gameObject().SetActive(true);
Vector3[] message_corners = message_item_w.worldCorners;
Vector3[] next_arrow_corners = next_arrow_w.worldCorners;
((UIRect)next_arrow_w).SetAnchor(null);
next_arrow_w.cachedTransform.set_position(new Vector3((next_arrow_corners[0].x + next_arrow_corners[2].x) * 0.5f, message_corners[0].y - (next_arrow_corners[1].y - next_arrow_corners[0].y) * 0.5f, next_arrow_corners[0].z));
SetMessageDragEnabled(true);
Object.Destroy(typewriter);
typewriter = null;
messageHeight = 0;
messageNum++;
coroutine = null;
}
private IEnumerator OpenQuestion()
{
sm.SetBGM("puzzle");
//恢复默认位置
QLabel.transform.localPosition = Vector3.zero;
QLabel.GetComponent <UILabel>().text = "Question " + reasoningEvent.num;
QLabel.SetActive(false);
//3 Question框打开
questionContainer.SetActive(true);
float t = 0, alpha = 0;
while (t < 1)
{
t = Mathf.MoveTowards(t, 1, 1 / 0.5f * Time.deltaTime);
float scale = 2 - t;
if (t < 0.5)
{
alpha = t * 2;
}
questionContainer.transform.localScale = new Vector3(scale, scale, 1);
questionContainer.GetComponent <UIWidget>().alpha = alpha;
yield return(null);
}
yield return(new WaitForSeconds(0.2f));
//显示question 并闪烁
QLabel.SetActive(true);
for (int i = 0; i < 4; i++)
{
QLabel.GetComponent <UILabel>().color = Color.gray;
yield return(new WaitForSeconds(0.1f));
QLabel.GetComponent <UILabel>().color = Color.white;
yield return(new WaitForSeconds(0.1f));
}
//移动Qustion到指定位置
t = 0;
while (t < 1)
{
t = Mathf.MoveTowards(t, 1, 1 / 0.2f * Time.deltaTime);
float x = t * -360;
QLabel.transform.localPosition = new Vector3(x, QLabel.transform.localPosition.y);
yield return(null);
}
//4 题目显示
questionLabel.GetComponent <UILabel>().text = reasoningEvent.question;
questionLabel.SetActive(true);
TypewriterEffect te = questionLabel.GetComponent <TypewriterEffect>();
te.ResetToBeginning();
while (te.isActive)
{
yield return(null);
}
yield return(new WaitForSeconds(0.5f));
//5 选项依次显示
StartCoroutine(ifevi ? ShowEvidence() : ShowChoice());
//6 *血条展示
hpmpManager.gameObject.SetActive(true);
hpmpManager.ShowBar(true, false);
}
void Start()
{
m_LeftEffect.ResetToBeginning();
m_RightEffect.ResetToBeginning();
}
void DialogUpdate(Local.StringLocalDialogInfo info)
{
string Name = info.NPCNameRIGHT;
//string talkername = "";
if (Name.Equals("0"))
{
Name = "";
}
if (Name.Contains("PLAYER"))
{
Name = Name.Replace("PLAYER", NetData.instance.Nickname);
}
//오른쪽
if (string.IsNullOrEmpty(Name) || info.TalkPosition == 0)
{
Labels[(int)labelT.MyName].gameObject.SetActive(false);
}
else
{
Labels[(int)labelT.MyName].gameObject.SetActive(true);
Labels[(int)labelT.MyName].text = Name;
}
if (info.TalkPosition == 2) // right
{
Labels[(int)labelT.MyName].depth = 5;
}
else
{
Labels[(int)labelT.MyName].depth = -5;
}
Name = info.NPCNameLEFT;
if (Name.Equals("0"))
{
Name = "";
}
if (Name.Contains("PLAYER"))
{
Name = Name.Replace("PLAYER", NetData.instance.Nickname);
}
//왼쪽
if (string.IsNullOrEmpty(Name) || info.TalkPosition == 0)
{
Labels[(int)labelT.TargetName].gameObject.SetActive(false);
}
else
{
Labels[(int)labelT.TargetName].gameObject.SetActive(true);
Labels[(int)labelT.TargetName].text = Name;
}
if (info.TalkPosition == 1) // left
{
Labels[(int)labelT.TargetName].depth = 5;
}
else
{
Labels[(int)labelT.TargetName].depth = -5;
}
string talk = info.String;
if (talk.Contains("\\\\\n"))
{
talk = talk.Replace("\\\\\n", "\n");
}
else if (talk.Contains("\\\\n"))
{
talk = talk.Replace("\\\\n", "\n");
}
else if (talk.Contains("\\\n"))
{
talk = talk.Replace("\\\n", "\n");
}
else if (talk.Contains("\\n"))
{
talk = talk.Replace("\\n", "\n");
}
typewriterEffect.ResetToBeginning(SceneManager.instance.IsPlaySoundFx, string.Format("{0}", talk));
}
