Digest() public method

Digests this protein into peptides.
public Digest ( IEnumerable proteases, int maxMissedCleavages = 3, int minLength = 1, int maxLength = int.MaxValue, bool initiatorMethonine = true, bool includeModifications = false, bool semiDigestion = false ) : IEnumerable
proteases IEnumerable The proteases to digest with
maxMissedCleavages int The max number of missed cleavages generated, 0 means no missed cleavages
minLength int The minimum length (in amino acids) of the peptide
maxLength int The maximum length (in amino acids) of the peptide
initiatorMethonine bool
includeModifications bool
semiDigestion bool
return IEnumerable
Protein Class Documentation
Ejemplo n.º 1
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        public void DuplicatePeptidesAreEqualivant()
        {
            Protein prot = new Protein("DEREKDEREK");

            var peptides = prot.Digest(Protease.GetProtease("LysC"), 0).ToList();
            Assert.AreEqual(peptides[0], peptides[1]);
        }
Ejemplo n.º 2
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        public void DuplicatePeptidesReturn()
        {
            Protein prot = new Protein("DEREKDEREK");

            var peptides = prot.Digest(Protease.GetProtease("LysC"), 0).ToList();
            Assert.AreEqual(peptides.Count, 2);
        }
Ejemplo n.º 3
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        public void SemiTrypiticDigestion()
        {
            Protein prot = new Protein("MMRGFKQRLIKKTTGSSSSSSSKKKDKEKEKEKSSTTSSTSKKPASASSSSHGTTHSSASSTGSKSTTEKGKQSGSVPSQ");

            var peptides = prot.Digest(Protease.GetProtease("Trypsin"), 0, 5, 10, semiDigestion: true).ToList();

            Assert.AreEqual(17, peptides.Count);
        }