private static ResultsTestDocumentContainer MinimizeCacheFile(SrmDocument document,
ChromCacheMinimizer.Settings settings, string skyFilePath)
{
Assert.IsNotNull(skyFilePath); // For ReSharper
string skydFilePath = Path.ChangeExtension(skyFilePath, ChromatogramCache.EXT);
ChromCacheMinimizer chromCacheMinimizer = document.Settings.MeasuredResults.GetChromCacheMinimizer(document);
using (var fs = new FileSaver(skydFilePath))
using (var fsScans = new FileSaver(skydFilePath + ChromatogramCache.SCANS_EXT, true))
using (var fsPeaks = new FileSaver(skydFilePath + ChromatogramCache.PEAKS_EXT, true))
using (var fsScores = new FileSaver(skydFilePath + ChromatogramCache.SCORES_EXT, true))
{
fs.Stream = File.OpenWrite(fs.SafeName);
chromCacheMinimizer.Minimize(settings, null, fs.Stream,
fsScans.FileStream, fsPeaks.FileStream, fsScores.FileStream);
fs.Commit();
}
using (var writer = new XmlTextWriter(skyFilePath, Encoding.UTF8)
{
Formatting = Formatting.Indented
})
{
var ser = new XmlSerializer(typeof(SrmDocument));
ser.Serialize(writer, document);
writer.Flush();
writer.Close();
}
var container = new ResultsTestDocumentContainer(document, skyFilePath, false);
Assert.IsTrue(container.SetDocument(ResultsUtil.DeserializeDocument(skyFilePath), document, true));
return(container);
}
public void TestCollectStatistics()
{
var testFilesDir = new TestFilesDir(TestContext, ZIP_FILE);
string docPath = testFilesDir.GetTestPath("BSA_Protea_label_free_20100323_meth3_multi.sky");
SrmDocument doc = ResultsUtil.DeserializeDocument(docPath);
var docContainer = new ResultsTestDocumentContainer(doc, docPath);
// Import the first RAW file (or mzML for international)
string rawPath = testFilesDir.GetTestPath("ah_20101011y_BSA_MS-MS_only_5-2" +
ExtensionTestContext.ExtThermoRaw);
var measuredResults = new MeasuredResults(new[] { new ChromatogramSet("Single", new[] { MsDataFileUri.Parse(rawPath) }) });
SrmDocument docResults = docContainer.ChangeMeasuredResults(measuredResults, 3, 3, 21);
ChromCacheMinimizer chromCacheMinimizer =
docResults.Settings.MeasuredResults.GetChromCacheMinimizer(docResults);
ChromCacheMinimizer.Settings settings =
new ChromCacheMinimizer.Settings().SetDiscardUnmatchedChromatograms(true);
ChromCacheMinimizer.MinStatistics minStatistics = null;
chromCacheMinimizer.Minimize(settings, s => minStatistics = s, null);
Assert.AreEqual(100, minStatistics.PercentComplete);
Assert.AreEqual(1.0, minStatistics.MinimizedRatio);
var docMissingFirstPeptide =
(SrmDocument)
docResults.ReplaceChild(
docResults.PeptideGroups.First().RemoveChild(docResults.PeptideGroups.First().Children[0]));
var docWithOnlyFirstPeptide =
(SrmDocument)
docResults.ReplaceChild(
docResults.PeptideGroups.First().ChangeChildren(new[] { docResults.PeptideGroups.First().Children[0] }));
ChromCacheMinimizer.MinStatistics statsMissingFirstProtein = null;
ChromCacheMinimizer.MinStatistics statsWithOnlyFirstProtein = null;
settings = settings.SetDiscardUnmatchedChromatograms(true);
ChromCacheMinimizer minimizerMissingFirstProtein =
docMissingFirstPeptide.Settings.MeasuredResults.GetChromCacheMinimizer(docMissingFirstPeptide);
ChromCacheMinimizer minimizerWithOnlyFirstProtein =
docWithOnlyFirstPeptide.Settings.MeasuredResults.GetChromCacheMinimizer(docWithOnlyFirstPeptide);
minimizerMissingFirstProtein.Minimize(settings, s => statsMissingFirstProtein = s, null);
minimizerWithOnlyFirstProtein.Minimize(settings, s => statsWithOnlyFirstProtein = s, null);
Assert.AreEqual(100, statsMissingFirstProtein.PercentComplete);
Assert.AreEqual(100, statsWithOnlyFirstProtein.PercentComplete);
Assert.AreEqual(1.0, statsMissingFirstProtein.MinimizedRatio + statsWithOnlyFirstProtein.MinimizedRatio,
.00001);
settings = settings.SetDiscardUnmatchedChromatograms(false);
ChromCacheMinimizer.MinStatistics statsMissingFirstProteinKeepAll = null;
ChromCacheMinimizer.MinStatistics statsWithOnlyFirstProteinKeepAll = null;
minimizerMissingFirstProtein.Minimize(settings, s => statsMissingFirstProteinKeepAll = s, null);
minimizerWithOnlyFirstProtein.Minimize(settings, s => statsWithOnlyFirstProteinKeepAll = s, null);
Assert.AreEqual(100, statsMissingFirstProteinKeepAll.PercentComplete);
Assert.AreEqual(1.0, statsMissingFirstProteinKeepAll.MinimizedRatio);
Assert.AreEqual(100, statsWithOnlyFirstProteinKeepAll.PercentComplete);
Assert.AreEqual(1.0, statsWithOnlyFirstProteinKeepAll.MinimizedRatio);
docContainer.Release();
}
