private void RefreshStatus()
{
if (File.Exists(textPath.Text))
{
btnAddFastaFile.Enabled = true;
ProteomeDb proteomeDb = null;
int proteinCount = 0;
try
{
using (var longWaitDlg = new LongWaitDlg
{
Text = Resources.BuildBackgroundProteomeDlg_RefreshStatus_Loading_Proteome_File,
Message =
string.Format(
Resources.BuildBackgroundProteomeDlg_RefreshStatus_Loading_protein_information_from__0__,
textPath.Text)
})
{
longWaitDlg.PerformWork(this, 1000, () =>
{
proteomeDb = ProteomeDb.OpenProteomeDb(textPath.Text);
if (proteomeDb != null)
{
proteinCount = proteomeDb.GetProteinCount(); // This can be a lengthy operation on a large protdb, do it within the longwait
}
});
}
if (proteomeDb == null)
{
throw new Exception();
}
tbxStatus.Text =
string.Format(
Resources.BuildBackgroundProteomeDlg_RefreshStatus_The_proteome_file_contains__0__proteins,
proteinCount);
}
catch (Exception)
{
tbxStatus.Text = Resources.BuildBackgroundProteomeDlg_OkDialog_The_proteome_file_is_not_valid;
btnAddFastaFile.Enabled = false;
}
finally
{
if (null != proteomeDb)
{
proteomeDb.Dispose();
}
}
textPath.ForeColor = Color.Black;
}
else
{
btnAddFastaFile.Enabled = false;
tbxStatus.Text = Resources.BuildBackgroundProteomeDlg_RefreshStatus_Click_the_Open_button_to_choose_an_existing_proteome_file_or_click_the_Create_button_to_create_a_new_proteome_file;
textPath.ForeColor = string.IsNullOrEmpty(textPath.Text) ? Color.Black : Color.Red;
}
}
private void RefreshStatus()
{
if (File.Exists(textPath.Text))
{
btnAddFastaFile.Enabled = true;
ProteomeDb proteomeDb = null;
try
{
using (var longWaitDlg = new LongWaitDlg
{
Text = Resources.BuildBackgroundProteomeDlg_RefreshStatus_Loading_Proteome_File,
Message =
string.Format(
Resources.BuildBackgroundProteomeDlg_RefreshStatus_Loading_protein_information_from__0__,
textPath.Text)
})
{
longWaitDlg.PerformWork(this, 1000, () => proteomeDb = ProteomeDb.OpenProteomeDb(textPath.Text));
}
if (proteomeDb == null)
{
throw new Exception();
}
int proteinCount = proteomeDb.GetProteinCount();
var digestions = proteomeDb.ListDigestions();
tbxStatus.Text =
string.Format(
Resources.BuildBackgroundProteomeDlg_RefreshStatus_The_proteome_file_contains__0__proteins,
proteinCount);
if (proteinCount != 0 && digestions.Count > 0)
{
tbxStatus.Text = TextUtil.LineSeparate(tbxStatus.Text,
Resources.BuildBackgroundProteomeDlg_RefreshStatus_The_proteome_has_already_been_digested);
}
}
catch (Exception)
{
tbxStatus.Text = Resources.BuildBackgroundProteomeDlg_OkDialog_The_proteome_file_is_not_valid;
btnAddFastaFile.Enabled = false;
}
finally
{
if (null != proteomeDb)
{
proteomeDb.Dispose();
}
}
}
else
{
btnAddFastaFile.Enabled = false;
tbxStatus.Text = Resources.BuildBackgroundProteomeDlg_RefreshStatus_Click_the_Open_button_to_choose_an_existing_proteome_file_or_click_the_Create_button_to_create_a_new_proteome_file;
}
}
public void DoTestOlderProteomeDb(TestContext testContext, bool doActualWebAccess)
{
using (var testFilesDir = new TestFilesDir(testContext, ZIP_FILE))
{
string fastaPath = testFilesDir.GetTestPath("tiny.fasta");
string protDbPath = testFilesDir.GetTestPath("celegans_mini.protdb"); // a version 0 protdb file
string blibPath = testFilesDir.GetTestPath("random.blib"); // a bibliospec file
// What happens when you try to open a random file as a protdb file?
AssertEx.ThrowsException <DbException>(() => ProteomeDb.OpenProteomeDb(fastaPath));
// What happens when you try to open a non-protdb database file as a protdb file?
AssertEx.ThrowsException <FileLoadException>(() => ProteomeDb.OpenProteomeDb(blibPath));
using (ProteomeDb proteomeDb = ProteomeDb.OpenProteomeDb(protDbPath))
{
Assert.IsTrue(proteomeDb.GetSchemaVersionMajor() == 0); // the initial db from our zipfile should be ancient
Assert.IsTrue(proteomeDb.GetSchemaVersionMinor() == 0); // the initial db from our zipfile should be ancient
Assert.AreEqual(9, proteomeDb.GetProteinCount());
var protein = proteomeDb.GetProteinByName("Y18D10A.20");
Assert.IsNotNull(protein);
Assert.IsTrue(String.IsNullOrEmpty(protein.Accession)); // old db won't have this populated
WebEnabledFastaImporter searcher = new WebEnabledFastaImporter(doActualWebAccess ? null :new WebEnabledFastaImporter.FakeWebSearchProvider());
bool searchComplete;
IProgressStatus status = new ProgressStatus(string.Empty);
Assert.IsTrue(proteomeDb.LookupProteinMetadata(new SilentProgressMonitor(), ref status, searcher, false, out searchComplete)); // add any missing protein metadata
Assert.IsTrue(searchComplete);
protein = proteomeDb.GetProteinByName("Y18D10A.20");
Assert.IsNotNull(protein);
if (doActualWebAccess) // We can actually go to the web for metadata
{
Assert.AreEqual("Q9XW16", protein.Accession);
}
using (var reader = new StreamReader(fastaPath))
{
proteomeDb.AddFastaFile(reader, new SilentProgressMonitor(), ref status, false);
}
// the act of writing should update to the current version
Assert.AreEqual(ProteomeDb.SCHEMA_VERSION_MAJOR_CURRENT, proteomeDb.GetSchemaVersionMajor());
Assert.AreEqual(ProteomeDb.SCHEMA_VERSION_MINOR_CURRENT, proteomeDb.GetSchemaVersionMinor());
Assert.AreEqual(19, proteomeDb.GetProteinCount());
// check for propery processed protein metadata
Assert.IsTrue(proteomeDb.LookupProteinMetadata(new SilentProgressMonitor(), ref status, searcher, false, out searchComplete));
Assert.IsTrue(searchComplete);
protein = proteomeDb.GetProteinByName("IPI00000044");
Assert.IsNotNull(protein);
Assert.AreEqual("P01127", protein.Accession); // We get this offline with our ipi->uniprot mapper
if (doActualWebAccess)
{
Assert.AreEqual("PDGFB_HUMAN", protein.PreferredName); // But this we get only with web access
}
/*
* // TODO(bspratt): fix "GetDigestion has no notion of a Db that has been added to, doesn't digest the new proteins and returns immediately (issue #304)"
* Enzyme trypsin = EnzymeList.GetDefault();
* proteomeDb.Digest(trypsin, new SilentProgressMonitor());
* Digestion digestion = proteomeDb.GetDigestion(trypsin.Name);
* var digestedProteins0 = digestion.GetProteinsWithSequencePrefix("EDGWVK", 100);
* Assert.IsTrue(digestedProteins0.Count >= 1);
* */
}
}
}