public void TestProteomeDb()
        {
            using (var testFilesDir = new TestFilesDir(TestContext, ZIP_FILE))
            {
                string fastaPath  = testFilesDir.GetTestPath("high_ipi.Human.20060111.fasta");
                string protDbPath = testFilesDir.GetTestPath("test.protdb");

                using (ProteomeDb proteomeDb = ProteomeDb.CreateProteomeDb(protDbPath))
                {
                    Enzyme trypsin = EnzymeList.GetDefault();
                    using (var reader = new StreamReader(fastaPath))
                    {
                        proteomeDb.AddFastaFile(reader, (msg, progress) => true);
                    }
                    // perform digestion
                    proteomeDb.Digest(new ProteaseImpl(trypsin), (msg, progress) => true);
                    Digestion digestion         = proteomeDb.GetDigestion(trypsin.Name);
                    var       digestedProteins0 = digestion.GetProteinsWithSequencePrefix("EDGWVK", 100);
                    Assert.IsTrue(digestedProteins0.Count >= 1);
                }
            }
        }
Beispiel #2
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        public void TestProteomeDb()
        {
            using (var testFilesDir = new TestFilesDir(TestContext, ZIP_FILE))
            {
                string fastaPath  = testFilesDir.GetTestPath("high_ipi.Human.20060111.fasta");
                string protDbPath = testFilesDir.GetTestPath("test.protdb");

                using (ProteomeDb proteomeDb = ProteomeDb.CreateProteomeDb(protDbPath))
                {
                    Enzyme          trypsin = EnzymeList.GetDefault();
                    IProgressStatus status  = new ProgressStatus(string.Empty);
                    using (var reader = new StreamReader(fastaPath))
                    {
                        proteomeDb.AddFastaFile(reader, new SilentProgressMonitor(), ref status, true); // Delay indexing
                    }
                    // perform digestion
                    proteomeDb.Digest(new ProteaseImpl(trypsin), ProteomeDb.PROTDB_MAX_MISSED_CLEAVAGES, new SilentProgressMonitor(), ref status);
                    Digestion digestion         = proteomeDb.GetDigestion(trypsin.Name);
                    var       digestedProteins0 = digestion.GetProteinsWithSequencePrefix("EDGWVK", 100);
                    Assert.IsTrue(digestedProteins0.Count >= 1);
                }
            }
        }