private void RunSimulation()
{
// For simple simulations, just use the simulator class.
// Interact with the HHModel directly (like this) only if you want to do a lot of tweaking.
Stopwatch stopwatch = Stopwatch.StartNew();
double stepSizeMsec = .01;
double sampleRate = 1.0 / stepSizeMsec;
double simulationLengthMsec = (double)nudDurationMs.Value;
int pointCount = (int)(simulationLengthMsec / stepSizeMsec);
double[] voltage = new double[pointCount];
double[] stateH = new double[pointCount];
double[] stateM = new double[pointCount];
double[] stateN = new double[pointCount];
double[] INa = new double[pointCount];
double[] IK = new double[pointCount];
double[] IKleak = new double[pointCount];
double[] stimulus = GenerateStimulusWaveform(pointCount);
var hh = new HHModel
{
ENa = (double)nudENa.Value,
EK = (double)nudEK.Value,
EKleak = (double)nudEKLeak.Value,
gNa = (double)nudGNa.Value,
gK = (double)nudGK.Value,
gKleak = (double)nudGKLeak.Value,
Cm = (double)nudCm.Value
};
for (int i = 0; i < pointCount; i++)
{
hh.StepForward(stimulus[i], stepSizeMsec);
voltage[i] = hh.Vm;
stateH[i] = hh.h.activation;
stateM[i] = hh.m.activation;
stateN[i] = hh.n.activation;
INa[i] = hh.INa;
IK[i] = hh.IK;
IKleak[i] = hh.IKleak;
}
double elapsedSec = (double)stopwatch.ElapsedTicks / Stopwatch.Frequency;
string message = string.Format("Simulated {2:n0} time points\nin {0:0.00} ms ({1:0.00} Hz)",
elapsedSec * 1000.0, 1 / elapsedSec, pointCount);
rtbStatus.Text = message;
formsPlot1.plt.Title("Membrane Potential (Vm)");
formsPlot1.plt.YLabel("Potential (mV)");
formsPlot1.plt.Clear();
formsPlot1.plt.PlotSignal(voltage, sampleRate, yOffset: -70, color: Color.Blue);
formsPlot1.plt.AxisAuto();
formsPlot1.Render();
formsPlot3.plt.Title("Stimulus");
formsPlot3.plt.YLabel("Current (µA/cm²)");
formsPlot3.plt.Clear();
formsPlot3.plt.PlotSignal(stimulus, sampleRate, color: Color.Red);
formsPlot3.plt.AxisAuto();
formsPlot3.Render();
formsPlot2.plt.Clear();
if (rbDisplayActivity.Checked == true)
{
formsPlot2.plt.Title("Voltage-Dependent Gate Activation");
formsPlot2.plt.YLabel("Activation (frac)");
formsPlot2.plt.PlotSignal(stateH, sampleRate, label: "h");
formsPlot2.plt.PlotSignal(stateM, sampleRate, label: "m");
formsPlot2.plt.PlotSignal(stateN, sampleRate, label: "n");
}
else if (rbDisplayCurrent.Checked == true)
{
formsPlot2.plt.Title("Channel Current");
formsPlot2.plt.YLabel("Current (µA/cm²)");
formsPlot2.plt.PlotSignal(INa, sampleRate, label: "VGSC");
formsPlot2.plt.PlotSignal(IK, sampleRate, label: "VGKC");
formsPlot2.plt.PlotSignal(IKleak, sampleRate, label: "Kleak");
}
else
{
throw new NotImplementedException();
}
formsPlot2.plt.XLabel("Simulation Time (milliseconds)");
formsPlot2.plt.Legend();
formsPlot2.plt.AxisAuto();
formsPlot2.Render();
}
