public static void ProcessRequest(object TheInfo)
{
try
{
RankSplitInfo info = TheInfo as RankSplitInfo;
string fName = LastFileName;
bool keepGoing = true;
bool seqMatch = false;
RankMethodSubSequence = info.SeqDiv;
OutputMethodSubSequence = info.SeqDivOut;
if (RankMethodSubSequence == SubSequenceDivision.Normalised)
{
RankStartDiv = info.divStart;
RankEndDiv = info.divEnd;
}
if (OutputMethodSubSequence == SubSequenceDivision.Normalised)
{
OutputStartDiv = info.divStartOut;
OutputEndDiv = info.divEndOut;
}
if (RankMethodSubSequence == SubSequenceDivision.AbsoluteBases)
{
RankBaseCount = info.baseCount;
RankFromStart = info.fromStart;
}
if (OutputMethodSubSequence == SubSequenceDivision.AbsoluteBases)
{
OutputFromStart = info.fromStartOut;
OutputBaseCount = info.baseCountOut;
}
if (info.GraphingType == GraphType.Image && OutputMethodSubSequence != SubSequenceDivision.AbsoluteBases)
{
OutputMethodSubSequence = SubSequenceDivision.AbsoluteBases;
OutputFromStart = !AppSettings.Output.DEFAULT_IMG_FROMEND.Item;
OutputBaseCount = AppSettings.Output.DEFAULT_IMG_BASES.Item;
}
if (info.sample != "All")
{
SampleToUse = info.sample;
UseSample = true;
}
else
{
UseSample = false;
}
if (info.SeqFeature == info.SeqFeature2)
{
seqMatch = true;
}
else
{
seqMatch = false;
}
if (keepGoing)
{
RSplitContainer <string> rspcString = new RSplitContainer <string> ("none", false);
RSplitContainer <double> rspcDouble = new RSplitContainer <double> (0, false);
RSplitContainer <List <double> > rspcListDouble = new RSplitContainer <List <double> > (null, true);
Dictionary <string, double> rankDict = Methods [info.RMethod].Invoke(info.SeqFeature);
Dictionary <string, string> baseDict;
Dictionary <string, double> singleDict;
Dictionary <string, List <double> > listDict;
if (info.doGraph == false)
{
switch (info.Text)
{
case OutputMethText.BlastID:
baseDict = MainData.Genome.RSPOUTBlastHitID(info.SeqFeature);
rspcString.MergeDictosToList(rankDict, baseDict, true, seqMatch);
ProcessingClass.WriteDataFile(fName, rspcString.GetData(false));
break;
case OutputMethText.BaseSeq:
baseDict = MainData.Genome.RSPOUTBaseSeq(info.SeqFeature);
rspcString.MergeDictosToList(rankDict, baseDict, false, seqMatch);
ProcessingClass.WriteDataFile(fName, rspcString.GetData(false));
break;
case OutputMethText.FastaBase:
baseDict = MainData.Genome.RSPOUTBaseSeq(info.SeqFeature);
rspcString.MergeDictosToList(rankDict, baseDict, false, seqMatch);
rspcString.WriteFastaFile(fName);
break;
case OutputMethText.SeqNames:
baseDict = MainData.Genome.RSPOUTReadName(info.SeqFeature);
rspcString.MergeDictosToList(rankDict, baseDict, false, seqMatch);
ProcessingClass.WriteDataFile(fName, rspcString.GetData(false));
break;
default:
break;
}
}
else
{
string yLab; string xLab;
SetLabels(info.Graph, info.GraphingType, out xLab, out yLab);
string title = info.SeqFeature + " ";
if (info.doSubSeq)
{
title += "from ";
if (info.SeqDiv == SubSequenceDivision.AbsoluteBases)
{
title += info.baseCount + " ";
if (info.fromStart)
{
title += "from start ";
}
else
{
title += "to end ";
}
}
else
{
title += "division " + info.divStart + "/20 to " + info.divEnd + "/20 ";
}
}
title += "ranked by " + MethodDesc [info.RMethod] + " and split into " + info.SplitCount + " groups";
Dictionary <string, List <double> > procCont;
Dictionary <string, List <List <double> > > procContList;
switch (info.Graph)
{
case OutputMethGraph.Expr:
singleDict = MainData.Genome.RSPOUTExprValue(info.SeqFeature2);
procCont = ProcessRSplitContainer <double> (rspcDouble, info, rankDict, singleDict, seqMatch);
if (info.GraphingType == GraphType.Histo)
{
MainData.MakeMultiHistoRankGraph(procCont, GraphWindow.Global, xLab, yLab, title, rspcDouble, info.MergeGraphs);
}
else
{
MainData.MakeRankLineGraph(procCont, GraphWindow.Global, xLab, yLab, title, rspcDouble, info.MergeGraphs);
}
break;
case OutputMethGraph.ExprNorm:
singleDict = MainData.Genome.RSPOUTExprNorm(info.SeqFeature2);
procCont = ProcessRSplitContainer <double> (rspcDouble, info, rankDict, singleDict, seqMatch);
if (info.GraphingType == GraphType.Histo)
{
MainData.MakeMultiHistoRankGraph(procCont, GraphWindow.Global, xLab, yLab, title, rspcDouble, info.MergeGraphs);
}
else
{
MainData.MakeRankLineGraph(procCont, GraphWindow.Global, xLab, yLab, title, rspcDouble, info.MergeGraphs);
}
break;
case OutputMethGraph.ExprVar:
singleDict = MainData.Genome.RSPOUTExprRange(info.SeqFeature2);
procCont = ProcessRSplitContainer <double> (rspcDouble, info, rankDict, singleDict, seqMatch);
if (info.GraphingType == GraphType.Histo)
{
MainData.MakeMultiHistoRankGraph(procCont, GraphWindow.Global, xLab, yLab, title, rspcDouble, info.MergeGraphs);
}
else
{
MainData.MakeRankLineGraph(procCont, GraphWindow.Global, xLab, yLab, title, rspcDouble, info.MergeGraphs);
}
break;
case OutputMethGraph.MethyCov:
if (info.GraphingType == GraphType.Spatial)
{
listDict = MainData.Genome.RSPOUTMethylCoverageDivision(info.SeqFeature2);
procContList = ProcessRSplitContainer <List <double> > (rspcListDouble, info, rankDict, listDict, seqMatch);
if (info.SeqDivOut != SubSequenceDivision.AbsoluteBases)
{
MainData.MakeMultiBayesRankGraph(procContList, GraphWindow.Global, xLab, yLab, title, rspcListDouble, info.MergeGraphs);
}
else
{
MainData.MakeMultiBayesBaseRankGraph(procContList, GraphWindow.Global, xLab, yLab, title, info.fromStartOut, rspcListDouble, info.MergeGraphs);
}
}
else if (info.GraphingType == GraphType.Means)
{
singleDict = MainData.Genome.RSPOUTQuantMethyCov(info.SeqFeature2);
procCont = ProcessRSplitContainer <double> (rspcDouble, info, rankDict, singleDict, seqMatch);
MainData.MakeRankLineGraph(procCont, GraphWindow.Global, xLab, yLab, title, rspcDouble, info.MergeGraphs);
}
else if (info.GraphingType == GraphType.Image)
{
//TODO: Make the image!
}
break;
case OutputMethGraph.MethyDepthCov:
if (info.GraphingType == GraphType.Spatial)
{
listDict = MainData.Genome.RSPOUTMethylCoverageDepthDivision(info.SeqFeature2);
procContList = ProcessRSplitContainer <List <double> > (rspcListDouble, info, rankDict, listDict, seqMatch);
if (info.SeqDivOut != SubSequenceDivision.AbsoluteBases)
{
MainData.MakeMultiBayesRankGraph(procContList, GraphWindow.Global, xLab, yLab, title, rspcListDouble, info.MergeGraphs);
}
else
{
MainData.MakeMultiBayesBaseRankGraph(procContList, GraphWindow.Global, xLab, yLab, title, info.fromStartOut, rspcListDouble, info.MergeGraphs);
}
}
else if (info.GraphingType == GraphType.Means)
{
singleDict = MainData.Genome.RSPOUTQuantMethyDepthCov(info.SeqFeature2);
procCont = ProcessRSplitContainer <double> (rspcDouble, info, rankDict, singleDict, seqMatch);
MainData.MakeRankLineGraph(procCont, GraphWindow.Global, xLab, yLab, title, rspcDouble, info.MergeGraphs);
}
else if (info.GraphingType == GraphType.Histo)
{
singleDict = MainData.Genome.RSPOUTMethylDepthTotal(info.SeqFeature2);
procCont = ProcessRSplitContainer <double> (rspcDouble, info, rankDict, singleDict, seqMatch);
MainData.MakeMultiHistoRankGraph(procCont, GraphWindow.Global, xLab, yLab, title, rspcDouble, info.MergeGraphs);
}
else if (info.GraphingType == GraphType.Image)
{
listDict = MainData.Genome.RSPOUTMethylCoverageDepthDivision(info.SeqFeature2);
procContList = ProcessRSplitContainer <List <double> > (rspcListDouble, info, rankDict, listDict, seqMatch);
MainData.MakeRegionChangeMap(procContList, rspcListDouble, info.MergeGraphs);
}
break;
case OutputMethGraph.BlastE:
singleDict = MainData.Genome.RSPBlastEValue(info.SeqFeature2);
procCont = ProcessRSplitContainer <double> (rspcDouble, info, rankDict, singleDict, seqMatch);
if (info.GraphingType == GraphType.Means)
{
MainData.MakeRankLineGraph(procCont, GraphWindow.Global, xLab, yLab, title, rspcDouble, info.MergeGraphs);
}
else
{
MainData.MakeMultiHistoRankGraph(procCont, GraphWindow.Global, xLab, yLab, title, rspcDouble, info.MergeGraphs);
}
break;
case OutputMethGraph.BlastID:
singleDict = MainData.Genome.RSPBlastPercID(info.SeqFeature2);
procCont = ProcessRSplitContainer <double> (rspcDouble, info, rankDict, singleDict, seqMatch);
if (info.GraphingType == GraphType.Means)
{
MainData.MakeRankLineGraph(procCont, GraphWindow.Global, xLab, yLab, title, rspcDouble, info.MergeGraphs);
}
else
{
MainData.MakeMultiHistoRankGraph(procCont, GraphWindow.Global, xLab, yLab, title, rspcDouble, info.MergeGraphs);
}
break;
case OutputMethGraph.Variant:
singleDict = MainData.Genome.RSPOUTQuantVar(info.SeqFeature2);
procCont = ProcessRSplitContainer <double> (rspcDouble, info, rankDict, singleDict, seqMatch);
if (info.GraphingType == GraphType.Means)
{
MainData.MakeRankLineGraph(procCont, GraphWindow.Global, xLab, yLab, title, rspcDouble, info.MergeGraphs);
}
else
{
MainData.MakeMultiHistoRankGraph(procCont, GraphWindow.Global, xLab, yLab, title, rspcDouble, info.MergeGraphs);
}
break;
default:
break;
}
}
}
}
catch (Exception e)
{
MainData.ShowMessageWindow(e.Message, true);
}
}