private void GenerateForBatch(string sOutput, int rackIndex, int sampleIndexInRack, List <DetectedInfo> heightsThisTime)
{
bool bNeedUseLastFour = NeedUseLastFour(sampleIndexInRack);
log.InfoFormat("rack index : {0}, start sample : {1}", rackIndex, sampleIndexInRack);
//batch index
int batchID = 1 + sampleIndexInRack / labwareSettings.tipCount;
int tipOffSet = GetTipOffSet(bNeedUseLastFour);
int ditiMask = 0;
for (int i = tipOffSet; i < tipOffSet + heightsThisTime.Count; i++)
{
ditiMask += (int)Math.Pow(2, i);
}
//create batch file
string suffix = ".gwl";
string sBatchFile = sOutput + string.Format("\\worklist{0}{1}", batchID, suffix);
if (File.Exists(sBatchFile))
{
File.Delete(sBatchFile);
}
FileStream fs = new FileStream(sBatchFile, FileMode.CreateNew);
StreamWriter sw = new StreamWriter(fs, Encoding.Default);
sw.WriteLine("W;");
string sNotifierFolder = Utility.GetExeFolder();
//0 get diti
WriteComment(string.Format("batch id is: {0}", batchID), sw);
sw.WriteLine(string.Format(breakPrefix + "GetDiti2({0},\"DiTi 1000ul LiHa\",0,0,10,70);", ditiMask));
//1 aspirate plasmas
double area = mappingCalculator.GetArea();
if (GlobalVars.Instance.IsRedCell)
{
pipettingSettings.dstbuffySlice = 0;
mixCommand mixCommand = new mixCommand();
List <int> vols = new List <int>();
heightsThisTime.ForEach(x => vols.Add((int)(mappingCalculator.GetVolumeFromHeight(x.Z1) - mappingCalculator.GetVolumeFromHeight(x.Z2))));
var strs = mixCommand.GenerateMixForBatch(rackIndex, sampleIndexInRack, vols);
strs.ForEach(s => sw.WriteLine(s));
return;
}
List <POINT> ptsAsp = positionGenerator.GetSrcWells(sampleIndexInRack, heightsThisTime.Count); //.GetSrcWellsForCertainSliceOfOneBatch(batchIndex);
int plasmaSlice = pipettingSettings.dstPlasmaSlice;
List <double> plasmaVols = new List <double>();
for (int slice = 0; slice < plasmaSlice; slice++)
{
string sExe = sNotifierFolder + string.Format("Notifier.exe Pipetting;{0};{1};{2}", rackIndex, batchID - 1, slice);
if (sNotifierFolder != "")
{
sw.WriteLine(string.Format(breakPrefix + "Execute(\"{0}\",2,\"\",2);", sExe));
}
WriteComment(string.Format("Processing slice: {0}, plasma part", slice + 1), sw);
plasmaVols = GenerateForSlice(slice, plasmaSlice, ptsAsp, rackIndex, sampleIndexInRack, heightsThisTime, sw);
if (GlobalVars.Instance.TrackBarcode)
{
barcodeTracker.Track(plasmaVols, slice);
}
}
//2 aspirate & dispense buffy
bool bhasBuffyCoat = pipettingSettings.dstbuffySlice > 0;//ResultReader.Instance.HasBuffyCoat();
bool inSameColumn = IsDstWellsInSameColumn(rackIndex, sampleIndexInRack, ptsAsp.Count);
int globalSampleIndex = GetGlobalSampleIndex(rackIndex, sampleIndexInRack);
//int secondRegionStartSampleIndex = GetEndIndexForFirstRegion(rackIndex, startSample) + 1;
if (bhasBuffyCoat)
{
string sExe = sNotifierFolder + string.Format("Notifier.exe Pipetting;{0};{1};{2}", rackIndex, batchID - 1, pipettingSettings.dstPlasmaSlice);
if (sNotifierFolder != "")
{
sw.WriteLine(string.Format(breakPrefix + "Execute(\"{0}\",2,\"\",2);", sExe));
}
//3 为buffy设置tipVolume
List <double> buffyvolumes = new List <double>();
if (bNeedUseLastFour)
{
buffyvolumes.AddRange(new List <double> {
0, 0, 0, 0
});
POINT ptZero = new POINT(0, 0);
ptsAsp.InsertRange(0, new List <POINT>()
{
ptZero, ptZero, ptZero, ptZero
});
}
for (int tipIndex = 0; tipIndex < heightsThisTime.Count; tipIndex++)
{
buffyvolumes.Add(10); //为了让sample tracking起作用，加10ul
}
int srcGrid = GetSrcGrid(rackIndex);
string strAspirateBuffy = GenerateAspirateCommand(ptsAsp, buffyvolumes, BB_Buffy, srcGrid, 0, labwareSettings.sourceWells);
sw.WriteLine(strAspirateBuffy);
//4 asp buffy using MSD
WriteMSDCommands(sw, heightsThisTime, tipOffSet);
//5 dispense buffy
if (inSameColumn)
{
WriteDispenseBuffy(rackIndex, sampleIndexInRack, heightsThisTime.Count, bNeedUseLastFour, sw);
}
else//need to dispense to different column
{
int endIndexFirstColumn = GetEndIndexForFirstColumn(rackIndex, sampleIndexInRack);
int firstColumnSampleCount = endIndexFirstColumn - rackIndex * labwareSettings.sourceWells - sampleIndexInRack + 1;
WriteDispenseBuffy(rackIndex, sampleIndexInRack, firstColumnSampleCount, bNeedUseLastFour, sw);
int secondColumnStartSampleIndex = endIndexFirstColumn + 1;
WriteDispenseBuffy(rackIndex, secondColumnStartSampleIndex, heightsThisTime.Count - firstColumnSampleCount, bNeedUseLastFour, sw);
}
}
sw.WriteLine(string.Format(breakPrefix + "DropDiti({0},{1},2,10,70,0);", ditiMask, labwareSettings.wasteGrid));
int endSampleID = rackIndex * 16 + sampleIndexInRack + heightsThisTime.Count;
if (endSampleID >= detectInfos.Count)
{
string sFinishedCommand = sNotifierFolder + string.Format("Notifier.exe Pipetting;true");
sw.WriteLine(string.Format(breakPrefix + "Execute(\"{0}\",2,\"\",2);", sFinishedCommand));
}
sw.Close();
}
private void GenerateForBatch(string sOutput,int rackIndex, int sampleIndexInRack, List<DetectedInfo> heightsThisTime)
{
bool bNeedUseLastFour = NeedUseLastFour(sampleIndexInRack);
log.InfoFormat("rack index : {0}, start sample : {1}", rackIndex, sampleIndexInRack);
//batch index
int batchID = 1+ sampleIndexInRack / labwareSettings.tipCount;
int tipOffSet = GetTipOffSet(bNeedUseLastFour);
int ditiMask = 0;
for (int i = tipOffSet; i < tipOffSet + heightsThisTime.Count; i++)
ditiMask += (int)Math.Pow(2, i);
//create batch file
string suffix = ".gwl";
string sBatchFile = sOutput + string.Format("\\worklist{0}{1}",batchID,suffix);
if (File.Exists(sBatchFile))
File.Delete(sBatchFile);
FileStream fs = new FileStream(sBatchFile, FileMode.CreateNew);
StreamWriter sw = new StreamWriter(fs, Encoding.Default);
sw.WriteLine("W;");
string sNotifierFolder = Utility.GetExeFolder();
//0 get diti
WriteComment(string.Format("batch id is: {0}", batchID), sw);
sw.WriteLine(string.Format(breakPrefix + "GetDiti2({0},\"DiTi 1000ul LiHa\",0,0,10,70);", ditiMask));
//1 aspirate plasmas
double area = mappingCalculator.GetArea();
if(GlobalVars.Instance.IsRedCell)
{
pipettingSetting.dstbuffySlice = 0;
mixCommand mixCommand = new mixCommand();
List<int> vols = new List<int>();
heightsThisTime.ForEach(x=>vols.Add((int)(mappingCalculator.GetVolumeFromHeight(x.Z1)-mappingCalculator.GetVolumeFromHeight(x.Z2))));
var strs = mixCommand.GenerateMixForBatch(rackIndex,sampleIndexInRack,vols);
strs.ForEach(s => sw.WriteLine(s));
}
List<POINT> ptsAsp = positionGenerator.GetSrcWells(sampleIndexInRack, heightsThisTime.Count); //.GetSrcWellsForCertainSliceOfOneBatch(batchIndex);
int plasmaSlice = pipettingSetting.dstPlasmaSlice;
List<double> plasmaVols = new List<double>();
for (int slice = 0; slice < plasmaSlice; slice++)
{
string sExe = sNotifierFolder + string.Format("Notifier.exe Pipetting;{0};{1};{2}", rackIndex,batchID-1, slice);
if(sNotifierFolder != "")
sw.WriteLine(string.Format(breakPrefix + "Execute(\"{0}\",2,\"\",2);", sExe));
WriteComment(string.Format("Processing slice: {0}, plasma part", slice + 1), sw);
plasmaVols = GenerateForSlice(slice, plasmaSlice,ptsAsp,rackIndex,sampleIndexInRack, heightsThisTime,sw);
if(GlobalVars.Instance.TrackBarcode)
barcodeTracker.Track(plasmaVols,slice);
}
//2 aspirate & dispense buffy
bool bhasBuffyCoat = pipettingSetting.dstbuffySlice > 0;//ResultReader.Instance.HasBuffyCoat();
bool inSameColumn = IsDstWellsInSameColumn(rackIndex, sampleIndexInRack, ptsAsp.Count);
int globalSampleIndex = GetGlobalSampleIndex(rackIndex, sampleIndexInRack);
//int secondRegionStartSampleIndex = GetEndIndexForFirstRegion(rackIndex, startSample) + 1;
if (bhasBuffyCoat)
{
string sExe = sNotifierFolder + string.Format("Notifier.exe Pipetting;{0};{1};{2}", rackIndex,batchID-1, pipettingSetting.dstPlasmaSlice);
if(sNotifierFolder != "")
sw.WriteLine(string.Format(breakPrefix + "Execute(\"{0}\",2,\"\",2);", sExe));
//3 为buffy设置tipVolume
List<double> buffyvolumes = new List<double>();
if(bNeedUseLastFour)
{
buffyvolumes.AddRange(new List<double> { 0, 0, 0, 0 });
POINT ptZero = new POINT(0, 0);
ptsAsp.InsertRange(0, new List<POINT>() { ptZero, ptZero, ptZero, ptZero });
}
for (int tipIndex = 0; tipIndex < heightsThisTime.Count; tipIndex++)
{
buffyvolumes.Add(10); //为了让sample tracking起作用，加10ul
}
int srcGrid = GetSrcGrid(rackIndex);
string strAspirateBuffy = GenerateAspirateCommand(ptsAsp, buffyvolumes, BB_Buffy, srcGrid, 0, labwareSettings.sourceWells);
sw.WriteLine(strAspirateBuffy);
//4 asp buffy using MSD
WriteMSDCommands(sw, heightsThisTime, tipOffSet);
//5 dispense buffy
if (inSameColumn)
{
WriteDispenseBuffy(rackIndex, sampleIndexInRack, heightsThisTime.Count,bNeedUseLastFour,false, sw);
}
else//need to dispense to different column
{
int endIndexFirstColumn = GetEndIndexForFirstColumn(rackIndex, sampleIndexInRack);
int firstColumnSampleCount = endIndexFirstColumn - rackIndex * labwareSettings.sourceWells - sampleIndexInRack + 1;
WriteDispenseBuffy(rackIndex, sampleIndexInRack, firstColumnSampleCount, bNeedUseLastFour, true, sw);
int secondColumnStartSampleIndex = endIndexFirstColumn + 1;
WriteDispenseBuffy(rackIndex, secondColumnStartSampleIndex, heightsThisTime.Count - firstColumnSampleCount, bNeedUseLastFour, true, sw);
}
}
sw.WriteLine(string.Format(breakPrefix + "DropDiti({0},{1},2,10,70,0);", ditiMask,labwareSettings.wasteGrid));
int endSampleID = rackIndex * 16 + sampleIndexInRack + heightsThisTime.Count;
if (endSampleID >= detectInfos.Count)
{
string sFinishedCommand = sNotifierFolder + string.Format("Notifier.exe Pipetting;true");
sw.WriteLine(string.Format(breakPrefix + "Execute(\"{0}\",2,\"\",2);", sFinishedCommand));
}
sw.Close();
}
