public static IResult <bool> Execute(Bitmap image) // Lernstichprobe wird im Kachel-Gitter geteilt und als Image importiert
{
var r = new Result <bool>();
r.Value = false;
using (var source = image.Clone() as Bitmap){
using (var gsp = new SharpAccessory.Imaging.Filters.ColorDeconvolution() //Grayscale prozessor wandelt das Bild in grayscale um
.Get2ndStain(source, SharpAccessory.Imaging.Filters.ColorDeconvolution.KnownStain.HaematoxylinDAB))
{
int count = 0;
foreach (var grayscalePixel in gsp.Pixels()) // Gehen durch das Bild und prüfen jeder Pixel,
{
if (grayscalePixel.V > RedThresholdMin && DabThresholdMax > grayscalePixel.V) //ob es diesen Kriterien entspricht
{
count++;
if (count > 20) //wenn es mehr als 20 "Objekte", die oben genannten Kriterien entsprechen, gefunden
// wird die Kachel entsprechend markiert.
{
r.Value = true;
break;
}
}
}
}
}
return(r);
}
// Pro Kachel
public static IResult <bool> Execute(Bitmap image)
{
// Console.WriteLine("ColorDeconvolution.Execute()");
var r = new Result <bool>();
using (var source = image.Clone() as Bitmap){
/*
* var gspR=new GrayscaleProcessor(source.Clone() as Bitmap,RgbToGrayscaleConversion.JustReds);
* gspR.Dispose();
* r.DebugBitmaps.Add(Tuple.Create(gspR.Bitmap,"Red"));
* r.DebugLayers.Add(createLayer(gspR.Bitmap,RedThreshold,"Red"));
* r.DebugVariables.Add("RedThreshold",RedThreshold);
*
* var gpDAB = new SharpAccessory.Imaging.Filters.ColorDeconvolution().Get2ndStain(
* source,
* SharpAccessory.Imaging.Filters.ColorDeconvolution.KnownStain.HaematoxylinDAB);
*
* gpDAB.Dispose();
* r.DebugBitmaps.Add(Tuple.Create(gpDAB.Bitmap,"DAB"));
* var dabLayer=createLayer(gpDAB.Bitmap,DabThreshold,"DAB");
* r.DebugLayers.Add(dabLayer);
* r.DebugVariables.Add("DabThreshold",DabThreshold);
* r.Value= 0 < dabLayer.Objects.Count;
*
* // sobald ein bissel (>1) hämoglobin drin sind, dann ist es betrachtungswürdig
*
*/
// my version
var gpDAB = new SharpAccessory.Imaging.Filters.ColorDeconvolution().Get2ndStain(
source,
SharpAccessory.Imaging.Filters.ColorDeconvolution.KnownStain.HaematoxylinDAB);
// grauwert 0-255, ab 40 ist es wohl hämoglobin
// tile ist 100x100 => 10.000 pixel, wenn es min 10 sind deren wert > schwellwert ist
// dann die kachel behalten
int counter = 0;
int schwellWert = 40;
for (int i = 0; i < gpDAB.Height; i++)
{
for (int j = 0; j < gpDAB.Width; j++)
{
if (gpDAB[i, j] > schwellWert && ++counter > 10)
{
r.Value = true;
return(r);
}
}
}
}
r.Value = false;
return(r);
}
private static ObjectLayer createLayer(Bitmap bitmap, int threshold, string name)
{
var map = new Map(bitmap.Width, bitmap.Height);
using (var gsp = new SharpAccessory.Imaging.Filters.ColorDeconvolution().Get2ndStain(bitmap, SharpAccessory.Imaging.Filters.ColorDeconvolution.KnownStain.HaematoxylinDAB)){
gsp.WriteBack = false;
foreach (var grayscalePixel in gsp.Pixels())
{
map[grayscalePixel.X, grayscalePixel.Y] = grayscalePixel.V > threshold?1u:0u;
}
}
var layer = new ConnectedComponentCollector().Execute(map);
layer.Name = name;
return(layer);
}
public static IResult <ObjectLayer> Detect(Bitmap image)
{
var result = new Result <ObjectLayer>();
using (var source = image.Clone() as Bitmap)
{
var gpDAB = new SharpAccessory.Imaging.Filters.ColorDeconvolution().Get2ndStain(source, SharpAccessory.Imaging.Filters.ColorDeconvolution.KnownStain.HaematoxylinDAB);
gpDAB.Dispose();
result.DebugBitmaps.Add(Tuple.Create(gpDAB.Bitmap, "DAB"));
var dabLayer = createLayer(gpDAB.Bitmap, DabThreshold, "DAB");
result.DebugLayers.Add(dabLayer);
result.DebugVariables.Add("DabThreshold", DabThreshold);
ObjectPixels.ProcessLayer(dabLayer);
var layer = dabLayer.CreateAbove((ImageObject io) => io.Features["ObjectPixels"].Value < 100);
layer.Name = "Bud Results";
result.DebugLayers.Add(layer);
result.Value = layer;
}
return(result);
}
public static IResult <bool> Execute(Bitmap image)
{
var r = new Result <bool>();
using (var source = image.Clone() as Bitmap){
var gspR = new GrayscaleProcessor(source.Clone() as Bitmap, RgbToGrayscaleConversion.JustReds);
gspR.Dispose();
r.DebugBitmaps.Add(Tuple.Create(gspR.Bitmap, "Red"));
r.DebugLayers.Add(createLayer(gspR.Bitmap, RedThreshold, "Red"));
r.DebugVariables.Add("RedThreshold", RedThreshold);
var gpDAB = new SharpAccessory.Imaging.Filters.ColorDeconvolution().Get2ndStain(source, SharpAccessory.Imaging.Filters.ColorDeconvolution.KnownStain.HaematoxylinDAB);
gpDAB.Dispose();
r.DebugBitmaps.Add(Tuple.Create(gpDAB.Bitmap, "DAB"));
var dabLayer = createLayer(gpDAB.Bitmap, DabThreshold, "DAB");
r.DebugLayers.Add(dabLayer);
r.DebugVariables.Add("DabThreshold", DabThreshold);
r.Value = 0 < dabLayer.Objects.Count;
}
return(r);
}
