// Constructor
public MainPage()
{
InitializeComponent();
// Sample code to localize the ApplicationBar
//BuildLocalizedApplicationBar();
ScanControl.ScanResultFound += ScanControl_ScanResultFound;
ScanControl.AutoFocus();
}
public FusionBridge(string debugMzML, string logDir, bool showConsoleLogs)
{
Logs = new List <string>();
LogDir = logDir;
ShowConsoleLogs = showConsoleLogs;
IFusionInstrumentAccessContainer fusionContainer = null;
if (!String.IsNullOrEmpty(debugMzML)) // create fake Fusion container that reads from mzML file
{
WriteLog("FusionBridge constructor called in debug mode");
WriteLog(string.Format("Reading scans from {0}", debugMzML));
fusionContainer = new MzMLFusionContainer(debugMzML);
}
else // needs license to connect to the real instrument
{
//// Use the Factory creation method to create a Fusion Access Container
WriteLog("FusionBridge constructor called");
fusionContainer = getIFusionInstrumentAccessContainer();
}
// Connect to the service by going 'online'
fusionContainer.StartOnlineAccess();
// Wait until the service is connected
// (better through the event, but this is nice and simple)
while (!fusionContainer.ServiceConnected)
{
;
}
// From the instrument container, get access to a particular instrument
InstrumentAccess = fusionContainer.Get(1);
ScanContainer = InstrumentAccess.GetMsScanContainer(0);
WriteLog("Detector class: " + ScanContainer.DetectorClass);
// Dump key-value pairs in cs.Values
WriteLog("Custom scan parameters: ");
InstrumentControl = InstrumentAccess.Control;
ScanControl = InstrumentControl.GetScans(false);
ICustomScan cs = ScanControl.CreateCustomScan();
foreach (KeyValuePair <string, string> kvp in cs.Values)
{
string kvpString = string.Format("- cs.Values\tKey = {0}, Value = {1}", kvp.Key, kvp.Value);
WriteLog(kvpString);
}
// Print instrument state
WriteLog("FusionBridge constructor initialised");
IState state = InstrumentControl.Acquisition.State;
string msg = string.Format("System mode = {0}, system state = {1}", state.SystemMode,
state.SystemState);
WriteLog(msg);
}
public bool CreateCustomScan(long runningNumber, double precursorMass, double isolationWidth, double collisionEnergy, int msLevel,
string polarity, double firstMass, double lastMass, double singleProcessingDelay)
{
WriteLog(String.Format("Placing a custom scan (runningNumber={0}, singleProcessingDelay={1})", runningNumber, singleProcessingDelay));
// TODO: validate input
if (ScanControl.PossibleParameters.Length > 0)
{
ICustomScan cs = ScanControl.CreateCustomScan();
cs.RunningNumber = runningNumber;
// Allow an extra delay of 500 ms, we will answer as fast as possible, so this is a maximum value.
cs.SingleProcessingDelay = singleProcessingDelay;
// Set the custom scan parameters
cs.Values["CollisionEnergy"] = collisionEnergy.ToString();
cs.Values["FirstMass"] = firstMass.ToString();
cs.Values["LastMass"] = lastMass.ToString();
cs.Values["Analyzer"] = "Orbitrap";
cs.Values["Polarity"] = polarity;
cs.Values["IsolationWidth"] = isolationWidth.ToString();
cs.Values["PrecursorMass"] = precursorMass.ToString();
if (msLevel == 1)
{
cs.Values["ScanType"] = "Full";
cs.Values["OrbitrapResolution"] = "120000";
}
else
{
cs.Values["ScanType"] = "MSn";
cs.Values["OrbitrapResolution"] = "7500";
}
cs.Values["ActivationType"] = "HCD";
cs.Values["DataType"] = "Centroid";
cs.Values["AGCTarget"] = "30000";
cs.Values["MaxIT"] = "100";
cs.Values["Microscans"] = "3";
// Dump key-value pairs in cs.Values
foreach (KeyValuePair <string, string> kvp in cs.Values)
{
string msg = string.Format("- cs.Values\tKey = {0}, Value = {1}", kvp.Key, kvp.Value);
WriteLog(msg);
}
try
{
if (!ScanControl.SetCustomScan(cs))
{
WriteLog(String.Format("FAILED to place a custom scan (runningNumber={0}): connection to service broken!!", cs.RunningNumber));
return(false);
}
else
{
if (cs.Values["ScanType"] == "Full")
{
WriteLog(String.Format("Successfully placed a custom fullscan scan (runningNumber={0})", cs.RunningNumber));
}
else if (cs.Values["ScanType"] == "MSn")
{
WriteLog(String.Format("Successfully placed a custom MSn scan (runningNumber={0})", cs.RunningNumber));
}
return(true);
}
}
catch (Exception e)
{
WriteLog(String.Format("FAILED to place a custom scan (runningNumber={0}): {1}", cs.RunningNumber, e.Message));
WriteLog(String.Format("Stacktrace: {0}", e.StackTrace));
throw;
}
}
else
{
WriteLog(String.Format("FAILED to place a custom scan (runningNumber={0}): no parameters available!!", runningNumber));
return(false);
}
}
