public void results_decoy_dataframe_with_something()
{
Sweet.lollipop = new Lollipop();
Sweet.lollipop.input_files.Add(ConstructorsForTesting.InputFile("fake.txt", Labeling.NeuCode, Purpose.Identification, "n", "s", "1", "1", "1")); //0
ExperimentalProteoform e = ConstructorsForTesting.ExperimentalProteoform("E1");
e.linked_proteoform_references = new List <Proteoform>(new List <Proteoform> {
ConstructorsForTesting.make_a_theoretical()
});
e.ptm_set = e.linked_proteoform_references.Last().ptm_set;
ProteoformFamily f = new ProteoformFamily(e);
f.construct_family();
Sweet.lollipop.decoy_proteoform_communities.Add("Decoy1", new ProteoformCommunity());
Sweet.lollipop.decoy_proteoform_communities["Decoy1"].families = new List <ProteoformFamily> {
f
};
var time_stamp = Sweet.time_stamp();
var directory = TestContext.CurrentContext.TestDirectory;
ResultsSummaryGenerator.save_all(TestContext.CurrentContext.TestDirectory, time_stamp, Sweet.lollipop.TusherAnalysis1 as IGoAnalysis, Sweet.lollipop.TusherAnalysis1 as TusherAnalysis);
Assert.IsTrue(File.Exists(Path.Combine(directory, "decoy_experimental_results_" + time_stamp + ".tsv")));
var lines = File.ReadAllLines(Path.Combine(directory, "decoy_experimental_results_" + time_stamp + ".tsv"));
Assert.AreEqual(2, lines.Length);
}
public void cytoscape_script_from_topdown()
{
Sweet.lollipop = new Lollipop();
ProteoformCommunity community = TestProteoformFamilies.construct_community_with_td_proteoforms(-1);
Sweet.lollipop.target_proteoform_community = community;
TopDownProteoform td = ConstructorsForTesting.TopDownProteoform("ASDF", 1000, 50);
td.gene_name = new GeneName(new List <Tuple <string, string> > {
new Tuple <string, string>("genename", "genename")
});
ProteoformFamily fam = new ProteoformFamily(td);
fam.construct_family();
CytoscapeScript.write_cytoscape_script(new List <ProteoformFamily>()
{
fam
}, new List <ProteoformFamily>()
{
fam
},
TestContext.CurrentContext.TestDirectory, "", "test",
null, false, false,
CytoscapeScript.color_scheme_names[0], Lollipop.edge_labels[0], Lollipop.node_labels[0], CytoscapeScript.node_label_positions[0], Lollipop.node_positioning[0], 2,
true, Lollipop.gene_name_labels[1]);
string[] edge_lines = File.ReadAllLines(Path.Combine(TestContext.CurrentContext.TestDirectory, CytoscapeScript.edge_file_prefix + "test" + CytoscapeScript.edge_file_extension));
HashSet <string> shared_pf_names_edges = new HashSet <string>();
for (int i = 1; i < edge_lines.Length; i++)
{
if (edge_lines[i] == "")
{
break;
}
string[] line = edge_lines[i].Split(new char[] { '\t' });
shared_pf_names_edges.Add(line[0]);
shared_pf_names_edges.Add(line[2]);
}
string[] node_lines = File.ReadAllLines(Path.Combine(TestContext.CurrentContext.TestDirectory, CytoscapeScript.node_file_prefix + "test" + CytoscapeScript.node_file_extension));
HashSet <string> shared_pf_names_nodes = new HashSet <string>();
for (int i = 1; i < node_lines.Length; i++)
{
if (node_lines[i] == "")
{
break;
}
string[] line = node_lines[i].Split(new char[] { '\t' });
shared_pf_names_nodes.Add(line[0]);
}
Assert.True(shared_pf_names_nodes.All(name => shared_pf_names_edges.Contains(name)));
Assert.True(shared_pf_names_edges.All(name => shared_pf_names_nodes.Contains(name)));
Assert.AreEqual(2, shared_pf_names_nodes.Count);
Assert.AreEqual(2, shared_pf_names_edges.Count);
}
private void display_family_members(int row_index, int column_index)
{
ProteoformFamily selected_family = (ProteoformFamily)((DisplayObject)this.dgv_main.Rows[row_index].DataBoundItem).display_object;
if (column_index < 0)
{
return;
}
if (new List <string> {
nameof(DisplayProteoformFamily.theoretical_count), nameof(DisplayProteoformFamily.accession_list), nameof(DisplayProteoformFamily.name_list)
}.Contains(dgv_main.Columns[column_index].Name))
{
if (selected_family.theoretical_proteoforms.Count > 0)
{
DisplayUtility.FillDataGridView(dgv_proteoform_family_members, selected_family.theoretical_proteoforms.Select(t => new DisplayTheoreticalProteoform(t)));
DisplayTheoreticalProteoform.FormatTheoreticalProteoformTable(dgv_proteoform_family_members);
}
else
{
dgv_proteoform_family_members.Rows.Clear();
}
}
else if (new List <string> {
nameof(DisplayProteoformFamily.experimental_count), nameof(DisplayProteoformFamily.experimentals_list), nameof(DisplayProteoformFamily.agg_mass_list)
}.Contains(dgv_main.Columns[column_index].Name))
{
if (selected_family.experimental_proteoforms.Count > 0)
{
DisplayUtility.FillDataGridView(dgv_proteoform_family_members, selected_family.experimental_proteoforms.Select(e => new DisplayExperimentalProteoform(e)));
DisplayExperimentalProteoform.FormatAggregatesTable(dgv_proteoform_family_members);
}
else
{
dgv_proteoform_family_members.Rows.Clear();
}
}
else if (dgv_main.Columns[column_index].Name == nameof(DisplayProteoformFamily.relation_count))
{
if (selected_family.relations.Count > 0)
{
DisplayUtility.FillDataGridView(dgv_proteoform_family_members, selected_family.relations.Select(r => new DisplayProteoformRelation(r)));
DisplayProteoformRelation.FormatRelationsGridView(dgv_proteoform_family_members, false, false);
}
else
{
dgv_proteoform_family_members.Rows.Clear();
}
}
}
public void results_dataframe_with_something()
{
Sweet.lollipop = new Lollipop();
Sweet.lollipop.input_files.Add(ConstructorsForTesting.InputFile("fake.txt", Labeling.NeuCode, Purpose.Identification, "n", "s", "1", "1", "1")); //0
ExperimentalProteoform e = ConstructorsForTesting.ExperimentalProteoform("E1");
e.linked_proteoform_references = new List <Proteoform>(new List <Proteoform> {
ConstructorsForTesting.make_a_theoretical()
});
e.ptm_set = e.linked_proteoform_references.Last().ptm_set;
ProteoformFamily f = new ProteoformFamily(e);
f.construct_family();
Sweet.lollipop.target_proteoform_community.families = new List <ProteoformFamily> {
f
};
string[] lines = ResultsSummaryGenerator.datatable_tostring(ResultsSummaryGenerator.experimental_results_dataframe(Sweet.lollipop.target_proteoform_community, Sweet.lollipop.TusherAnalysis1)).Split(new string[] { Environment.NewLine }, StringSplitOptions.None);
Assert.True(lines.Count() == 3);
Assert.True(lines.Any(a => a.Contains("E1")));
TopDownProteoform td = ConstructorsForTesting.TopDownProteoform("TD1", 1000, 10);
td.linked_proteoform_references = new List <Proteoform>(new List <Proteoform> {
ConstructorsForTesting.make_a_theoretical()
});
td.ptm_set = e.linked_proteoform_references.Last().ptm_set;
ProteoformFamily f2 = new ProteoformFamily(td);
f2.construct_family();
Sweet.lollipop.target_proteoform_community.families = new List <ProteoformFamily> {
f2
};
Sweet.lollipop.topdown_proteoforms = new List <TopDownProteoform>()
{
td
};
lines = ResultsSummaryGenerator.datatable_tostring(ResultsSummaryGenerator.experimental_results_dataframe(Sweet.lollipop.target_proteoform_community, Sweet.lollipop.TusherAnalysis1)).Split(new string[] { Environment.NewLine }, StringSplitOptions.None);
Assert.True(lines.Count() == 3);
lines = ResultsSummaryGenerator.datatable_tostring(ResultsSummaryGenerator.topdown_results_dataframe()).Split(new string[] { Environment.NewLine }, StringSplitOptions.None);
Assert.True(lines.Count() == 3);
Assert.True(lines.Any(a => a.Contains("TD1")));
Sweet.lollipop.target_proteoform_community.families = new List <ProteoformFamily> {
f, f2
};
lines = ResultsSummaryGenerator.datatable_tostring(ResultsSummaryGenerator.experimental_intensities_dataframe()).Split(new string[] { Environment.NewLine }, StringSplitOptions.None);
Assert.AreEqual(lines.Count(), 4);
Assert.True(lines.Any(a => a.Contains("E1")));
Assert.True(lines.Any(a => a.Contains("TD1")));
}
public void nodes_table_gives_meaningful_unmodified_theoreticals()
{
Proteoform p = ConstructorsForTesting.make_a_theoretical();
ProteoformFamily f = new ProteoformFamily(p);
f.construct_family();
string node_table = CytoscapeScript.get_cytoscape_nodes_tsv(new List <ProteoformFamily> {
f
},
null,
CytoscapeScript.color_scheme_names[0], Lollipop.edge_labels[0], Lollipop.node_labels[0], Lollipop.node_positioning[0], 2,
f.theoretical_proteoforms, false, Lollipop.gene_name_labels[1]);
Assert.True(node_table.Contains(CytoscapeScript.unmodified_theoretical_label));
Assert.AreNotEqual(f.theoretical_proteoforms[0].accession, CytoscapeScript.get_proteoform_shared_name(p, Lollipop.node_labels[0], 2));
}
public void nodes_table_gives_meaningful_topdown()
{
TopDownProteoform t = ConstructorsForTesting.TopDownProteoform("ACC", 999.99, 50);
ProteoformFamily f = new ProteoformFamily(t);
f.construct_family();
string node_table = CytoscapeScript.get_cytoscape_nodes_tsv(new List <ProteoformFamily> {
f
},
null,
CytoscapeScript.color_scheme_names[0], Lollipop.edge_labels[0], Lollipop.node_labels[0], Lollipop.node_positioning[0], 2,
f.theoretical_proteoforms, false, Lollipop.gene_name_labels[1]);
Assert.True(node_table.Contains("ACC"));
Assert.True(node_table.Contains("999.99"));
}
public void results_dataframe_with_something()
{
ExperimentalProteoform e = ConstructorsForTesting.ExperimentalProteoform("E1");
e.linked_proteoform_references = new List <Proteoform>(new List <Proteoform> {
ConstructorsForTesting.make_a_theoretical()
});
e.ptm_set = e.linked_proteoform_references.Last().ptm_set;
ProteoformFamily f = new ProteoformFamily(e);
f.construct_family();
SaveState.lollipop.target_proteoform_community.families = new List <ProteoformFamily> {
f
};
string[] lines = ResultsSummaryGenerator.results_dataframe().Split(new string[] { Environment.NewLine }, StringSplitOptions.None);
Assert.True(lines.Count() == 3);
Assert.True(lines.Any(a => a.Contains("E1")));
}
public void nodes_table_gives_meaningful_experimentals()
{
ExperimentalProteoform e = ConstructorsForTesting.ExperimentalProteoform("E1");
e.agg_intensity = 999.99;
e.agg_mass = 888.88;
e.agg_rt = 777.77;
ProteoformFamily f = new ProteoformFamily(e);
f.construct_family();
string node_table = CytoscapeScript.get_cytoscape_nodes_tsv(new List <ProteoformFamily> {
f
},
null,
CytoscapeScript.color_scheme_names[0], Lollipop.edge_labels[0], Lollipop.node_labels[0], Lollipop.node_positioning[0], 2,
f.theoretical_proteoforms, false, Lollipop.gene_name_labels[1]);
Assert.True(node_table.Contains("E1"));
Assert.True(node_table.Contains("999.99"));
}
public void nodes_table_gives_meaningful_modified_theoreticals2()
{
ModificationMotif motif;
ModificationMotif.TryGetMotif("K", out motif);
Modification m = new Modification("id", _modificationType: "modtype", _target: motif, _locationRestriction: "Anywhere.", _monoisotopicMass: 1);
Proteoform p = ConstructorsForTesting.make_a_theoretical();
ProteoformFamily f = new ProteoformFamily(p);
f.construct_family();
string node_table = CytoscapeScript.get_cytoscape_nodes_tsv(new List <ProteoformFamily> {
f
},
null,
CytoscapeScript.color_scheme_names[0], Lollipop.edge_labels[0], Lollipop.node_labels[0], Lollipop.node_positioning[0], 2,
f.theoretical_proteoforms, false, Lollipop.gene_name_labels[1]);
Assert.True(node_table.Contains(CytoscapeScript.modified_theoretical_label));
Assert.AreNotEqual(f.theoretical_proteoforms[0].accession, CytoscapeScript.get_proteoform_shared_name(p, Lollipop.node_labels[0], 2));
}
public void nodes_table_gives_meaningful_modified_theoreticals2()
{
ModificationMotif motif;
ModificationMotif.TryGetMotif("K", out motif);
ModificationWithMass m = new ModificationWithMass("oxidation", new Tuple <string, string>("", ""), motif, ModificationSites.K, 1, new Dictionary <string, IList <string> >(), new List <double>(), new List <double>(), "");
Proteoform p = ConstructorsForTesting.make_a_theoretical();
ProteoformFamily f = new ProteoformFamily(p);
f.construct_family();
string node_table = CytoscapeScript.get_cytoscape_nodes_tsv(new List <ProteoformFamily> {
f
},
false,
CytoscapeScript.color_scheme_names[0], Lollipop.edge_labels[0], Lollipop.node_labels[0], Lollipop.node_positioning[0], 2,
f.theoretical_proteoforms, false, Lollipop.gene_name_labels[1]);
Assert.True(node_table.Contains(CytoscapeScript.modified_theoretical_label));
Assert.AreNotEqual(f.theoretical_proteoforms[0].accession, CytoscapeScript.get_proteoform_shared_name(p, Lollipop.node_labels[0], 2));
}
public DisplayProteoformFamily(ProteoformFamily f)
: base(f)
{
this.f = f;
}
public void get_interesting_goterm_families()
{
Sweet.lollipop = new Lollipop();
Sweet.lollipop.theoretical_database.aaIsotopeMassList = new AminoAcidMasses(Sweet.lollipop.carbamidomethylation, Sweet.lollipop.neucode_labeled).AA_Masses;
Sweet.lollipop.significance_by_permutation = true;
Sweet.lollipop.significance_by_log2FC = false;
DatabaseReference d1 = new DatabaseReference("GO", "GO:1", new List <Tuple <string, string> > {
new Tuple <string, string>("term", "P:1")
});
DatabaseReference d2 = new DatabaseReference("GO", "GO:2", new List <Tuple <string, string> > {
new Tuple <string, string>("term", "P:2")
});
DatabaseReference d3 = new DatabaseReference("GO", "GO:1", new List <Tuple <string, string> > {
new Tuple <string, string>("term", "P:1")
});
GoTerm g1 = new GoTerm(d1);
GoTerm g2 = new GoTerm(d2);
GoTerm g3 = new GoTerm(d3);
ProteinWithGoTerms p1 = new ProteinWithGoTerms("ASDF", "T1", new List <Tuple <string, string> > {
new Tuple <string, string>("", "")
}, new Dictionary <int, List <Modification> >(), new List <ProteolysisProduct> {
new ProteolysisProduct(0, 0, "")
}, "T2", "T3", true, false, new List <DatabaseReference> {
d1
}, new List <GoTerm> {
g1
});
ProteinWithGoTerms p2 = new ProteinWithGoTerms("ASDF", "T2", new List <Tuple <string, string> > {
new Tuple <string, string>("", "")
}, new Dictionary <int, List <Modification> >(), new List <ProteolysisProduct> {
new ProteolysisProduct(0, 0, "")
}, "T2", "T3", true, false, new List <DatabaseReference> {
d2
}, new List <GoTerm> {
g2
});
ProteinWithGoTerms p3 = new ProteinWithGoTerms("ASDF", "T3", new List <Tuple <string, string> > {
new Tuple <string, string>("", "")
}, new Dictionary <int, List <Modification> >(), new List <ProteolysisProduct> {
new ProteolysisProduct(0, 0, "")
}, "T2", "T3", true, false, new List <DatabaseReference> {
d3
}, new List <GoTerm> {
g3
});
Dictionary <InputFile, Protein[]> dict = new Dictionary <InputFile, Protein[]>
{
{ new InputFile("fake.txt", Purpose.ProteinDatabase), new Protein[] { p1 } },
{ new InputFile("fake.txt", Purpose.ProteinDatabase), new Protein[] { p2 } },
{ new InputFile("fake.txt", Purpose.ProteinDatabase), new Protein[] { p3 } },
};
ExperimentalProteoform e1 = ConstructorsForTesting.ExperimentalProteoform("E");
ExperimentalProteoform e2 = ConstructorsForTesting.ExperimentalProteoform("E");
e1.quant.intensitySum = 1;
e1.quant.TusherValues1.significant = true;
e1.quant.tusherlogFoldChange = 1;
e2.quant.intensitySum = 1;
e2.quant.TusherValues1.significant = true;
e2.quant.tusherlogFoldChange = 1;
TheoreticalProteoform t = ConstructorsForTesting.make_a_theoretical("T1_T1_asdf", p1, dict);
TheoreticalProteoform u = ConstructorsForTesting.make_a_theoretical("T2_T1_asdf_asdf", p2, dict);
TheoreticalProteoform v = ConstructorsForTesting.make_a_theoretical("T3_T1_asdf_Asdf_Asdf", p3, dict);
t.ExpandedProteinList = new List <ProteinWithGoTerms> {
p1
};
u.ExpandedProteinList = new List <ProteinWithGoTerms> {
p2
};
v.ExpandedProteinList = new List <ProteinWithGoTerms> {
p3
};
t.begin = 1;
t.end = 1;
u.begin = 1;
u.end = 1;
v.begin = 1;
v.end = 1;
make_relation(e1, t);
//make_relation(e1, v); // we don't allow this to happen anymore... we only allow one ET conntection per E
make_relation(e2, u);
ProteoformFamily f = new ProteoformFamily(e1); // two theoreticals with the same GoTerms... expecting one GoTerm number but two theoretical proteins (now only one)
ProteoformFamily h = new ProteoformFamily(e2);
f.construct_family();
f.identify_experimentals();
h.construct_family();
h.identify_experimentals();
List <ProteoformFamily> families = new List <ProteoformFamily> {
f, h
};
t.family = f;
v.family = f;
e1.family = f;
u.family = h;
e2.family = h;
List <ExperimentalProteoform> fake_significant = new List <ExperimentalProteoform> {
e1
};
List <ProteinWithGoTerms> significant_proteins = Sweet.lollipop.getInducedOrRepressedProteins(fake_significant, Sweet.lollipop.TusherAnalysis1.GoAnalysis);
List <GoTermNumber> gtn = Sweet.lollipop.TusherAnalysis1.GoAnalysis.getGoTermNumbers(significant_proteins, new List <ProteinWithGoTerms> {
p1, p2, p3
});
Assert.AreEqual(1, significant_proteins.Count);
Assert.AreEqual(1, gtn.Count);
Assert.AreEqual("1", gtn.First().Id);
Assert.AreEqual(0 - (decimal)Math.Log(2d / 3d, 2), gtn.First().log_odds_ratio);
List <ProteoformFamily> fams = Sweet.lollipop.getInterestingFamilies(gtn, families);
Assert.AreEqual(1, fams.Count);
Assert.AreEqual(1, fams[0].theoretical_proteoforms.Count);
}
public void test_goterm_analysis_with_custom_list()
{
Sweet.lollipop = new Lollipop();
Sweet.lollipop.theoretical_database.aaIsotopeMassList = new AminoAcidMasses(Sweet.lollipop.carbamidomethylation, Sweet.lollipop.neucode_labeled).AA_Masses;
Sweet.lollipop.significance_by_permutation = true;
Sweet.lollipop.significance_by_log2FC = false;
DatabaseReference d1 = new DatabaseReference("GO", "GO:1", new List <Tuple <string, string> > {
new Tuple <string, string>("term", "P:1")
});
DatabaseReference d2 = new DatabaseReference("GO", "GO:2", new List <Tuple <string, string> > {
new Tuple <string, string>("term", "P:2")
});
DatabaseReference d3 = new DatabaseReference("GO", "GO:1", new List <Tuple <string, string> > {
new Tuple <string, string>("term", "P:1")
});
GoTerm g1 = new GoTerm(d1);
GoTerm g2 = new GoTerm(d2);
GoTerm g3 = new GoTerm(d3);
ProteinWithGoTerms p1 = new ProteinWithGoTerms("ASDF", "T1", new List <Tuple <string, string> > {
new Tuple <string, string>("", "")
}, new Dictionary <int, List <Modification> >(), new List <ProteolysisProduct> {
new ProteolysisProduct(0, 0, "")
}, "T2", "T3", true, false, new List <DatabaseReference> {
d1
}, new List <GoTerm> {
g1
});
ProteinWithGoTerms p2 = new ProteinWithGoTerms("ASDF", "T2", new List <Tuple <string, string> > {
new Tuple <string, string>("", "")
}, new Dictionary <int, List <Modification> >(), new List <ProteolysisProduct> {
new ProteolysisProduct(0, 0, "")
}, "T2", "T3", true, false, new List <DatabaseReference> {
d2
}, new List <GoTerm> {
g2
});
ProteinWithGoTerms p3 = new ProteinWithGoTerms("ASDF", "T3", new List <Tuple <string, string> > {
new Tuple <string, string>("", "")
}, new Dictionary <int, List <Modification> >(), new List <ProteolysisProduct> {
new ProteolysisProduct(0, 0, "")
}, "T2", "T3", true, false, new List <DatabaseReference> {
d3
}, new List <GoTerm> {
g3
});
Dictionary <InputFile, Protein[]> dict = new Dictionary <InputFile, Protein[]> {
{ new InputFile("fake.txt", Purpose.ProteinDatabase), new Protein[] { p1 } },
{ new InputFile("fake.txt", Purpose.ProteinDatabase), new Protein[] { p2 } },
{ new InputFile("fake.txt", Purpose.ProteinDatabase), new Protein[] { p3 } },
};
ExperimentalProteoform e1 = ConstructorsForTesting.ExperimentalProteoform("E");
ExperimentalProteoform e2 = ConstructorsForTesting.ExperimentalProteoform("E");
e1.quant.intensitySum = 1;
e1.quant.TusherValues1.significant = true;
e1.quant.tusherlogFoldChange = 1;
e2.quant.intensitySum = 1;
e2.quant.TusherValues1.significant = true;
e2.quant.tusherlogFoldChange = 1;
TheoreticalProteoform t = ConstructorsForTesting.make_a_theoretical("T1_T1_asdf", p1, dict);
TheoreticalProteoform u = ConstructorsForTesting.make_a_theoretical("T2_T1_asdf_asdf", p2, dict);
TheoreticalProteoform v = ConstructorsForTesting.make_a_theoretical("T3_T1_asdf_Asdf_Asdf", p3, dict);
t.ExpandedProteinList = new List <ProteinWithGoTerms> {
p1
};
u.ExpandedProteinList = new List <ProteinWithGoTerms> {
p2
};
v.ExpandedProteinList = new List <ProteinWithGoTerms> {
p3
};
make_relation(e1, t);
make_relation(e1, v);
make_relation(e2, u);
ProteoformFamily f = new ProteoformFamily(e1); // two theoreticals with the same GoTerms... expecting one GoTerm number but two theoretical proteins
ProteoformFamily h = new ProteoformFamily(e2);
f.construct_family();
f.identify_experimentals();
h.construct_family();
h.identify_experimentals();
List <ProteoformFamily> families = new List <ProteoformFamily> {
f, h
};
t.family = f;
v.family = f;
e1.family = f;
u.family = h;
e2.family = h;
Sweet.lollipop.TusherAnalysis1.inducedOrRepressedProteins = Sweet.lollipop.getInducedOrRepressedProteins(new List <ExperimentalProteoform> {
e1
}, Sweet.lollipop.TusherAnalysis1.GoAnalysis);
Sweet.lollipop.TusherAnalysis1.GoAnalysis.allTheoreticalProteins = true;
Sweet.lollipop.theoretical_database.expanded_proteins = new ProteinWithGoTerms[] { p1, p2, p3 };
Sweet.lollipop.TusherAnalysis1.GoAnalysis.backgroundProteinsList = Path.Combine(TestContext.CurrentContext.TestDirectory, "test_protein_list.txt");
Sweet.lollipop.TusherAnalysis1.GoAnalysis.GO_analysis(Sweet.lollipop.TusherAnalysis1.inducedOrRepressedProteins);
Assert.AreEqual(1, Sweet.lollipop.TusherAnalysis1.inducedOrRepressedProteins.Count); // only taking one ET connection by definition in forming ET relations; only one is used in identify theoreticals
Assert.AreEqual(1, Sweet.lollipop.TusherAnalysis1.GoAnalysis.goTermNumbers.Count);
Assert.AreEqual("1", Sweet.lollipop.TusherAnalysis1.GoAnalysis.goTermNumbers.First().Id);
Assert.AreEqual(0 - (decimal)Math.Log(2d / 3d, 2), Sweet.lollipop.TusherAnalysis1.GoAnalysis.goTermNumbers.First().log_odds_ratio);
List <ProteoformFamily> fams = Sweet.lollipop.getInterestingFamilies(Sweet.lollipop.TusherAnalysis1.GoAnalysis.goTermNumbers, families);
Assert.AreEqual(1, fams.Count);
Assert.AreEqual(2, fams[0].theoretical_proteoforms.Count);
}
public void get_interesting_goterm_families()
{
SaveState.lollipop = new Lollipop();
DatabaseReference d1 = new DatabaseReference("GO", "GO:1", new List <Tuple <string, string> > {
new Tuple <string, string>("term", "P:1")
});
DatabaseReference d2 = new DatabaseReference("GO", "GO:2", new List <Tuple <string, string> > {
new Tuple <string, string>("term", "P:2")
});
DatabaseReference d3 = new DatabaseReference("GO", "GO:1", new List <Tuple <string, string> > {
new Tuple <string, string>("term", "P:1")
});
GoTerm g1 = new GoTerm(d1);
GoTerm g2 = new GoTerm(d2);
GoTerm g3 = new GoTerm(d3);
ProteinWithGoTerms p1 = new ProteinWithGoTerms("", "T1", new List <Tuple <string, string> > {
new Tuple <string, string>("", "")
}, new Dictionary <int, List <Modification> >(), new int?[] { 0 }, new int?[] { 0 }, new string[] { "" }, "T2", "T3", true, false, new List <DatabaseReference> {
d1
}, new List <GoTerm> {
g1
});
ProteinWithGoTerms p2 = new ProteinWithGoTerms("", "T2", new List <Tuple <string, string> > {
new Tuple <string, string>("", "")
}, new Dictionary <int, List <Modification> >(), new int?[] { 0 }, new int?[] { 0 }, new string[] { "" }, "T2", "T3", true, false, new List <DatabaseReference> {
d2
}, new List <GoTerm> {
g2
});
ProteinWithGoTerms p3 = new ProteinWithGoTerms("", "T3", new List <Tuple <string, string> > {
new Tuple <string, string>("", "")
}, new Dictionary <int, List <Modification> >(), new int?[] { 0 }, new int?[] { 0 }, new string[] { "" }, "T2", "T3", true, false, new List <DatabaseReference> {
d3
}, new List <GoTerm> {
g3
});
Dictionary <InputFile, Protein[]> dict = new Dictionary <InputFile, Protein[]>
{
{ new InputFile("fake.txt", Purpose.ProteinDatabase), new Protein[] { p1 } },
{ new InputFile("fake.txt", Purpose.ProteinDatabase), new Protein[] { p2 } },
{ new InputFile("fake.txt", Purpose.ProteinDatabase), new Protein[] { p3 } },
};
ExperimentalProteoform e1 = ConstructorsForTesting.ExperimentalProteoform("E");
ExperimentalProteoform e2 = ConstructorsForTesting.ExperimentalProteoform("E");
e1.quant.intensitySum = 1;
e1.quant.FDR = 0;
e1.quant.logFoldChange = 1;
e2.quant.intensitySum = 1;
e2.quant.FDR = 0;
e2.quant.logFoldChange = 1;
TheoreticalProteoform t = ConstructorsForTesting.make_a_theoretical("T1_T1_asdf", p1, dict);
TheoreticalProteoform u = ConstructorsForTesting.make_a_theoretical("T2_T1_asdf_asdf", p2, dict);
TheoreticalProteoform v = ConstructorsForTesting.make_a_theoretical("T3_T1_asdf_Asdf_Asdf", p3, dict);
t.ExpandedProteinList = new List <ProteinWithGoTerms> {
p1
};
u.ExpandedProteinList = new List <ProteinWithGoTerms> {
p2
};
v.ExpandedProteinList = new List <ProteinWithGoTerms> {
p3
};
make_relation(e1, t);
//make_relation(e1, v); // we don't allow this to happen anymore... we only allow one ET conntection per E
make_relation(e2, u);
ProteoformFamily f = new ProteoformFamily(e1); // two theoreticals with the same GoTerms... expecting one GoTerm number but two theoretical proteins (now only one)
ProteoformFamily h = new ProteoformFamily(e2);
f.construct_family();
f.identify_experimentals();
h.construct_family();
h.identify_experimentals();
List <ProteoformFamily> families = new List <ProteoformFamily> {
f, h
};
t.family = f;
v.family = f;
e1.family = f;
u.family = h;
e2.family = h;
List <ExperimentalProteoform> fake_significant = new List <ExperimentalProteoform> {
e1
};
List <ProteinWithGoTerms> significant_proteins = SaveState.lollipop.getInducedOrRepressedProteins(fake_significant, 0, 1, 0);
List <GoTermNumber> gtn = SaveState.lollipop.getGoTermNumbers(significant_proteins, new List <ProteinWithGoTerms> {
p1, p2, p3
});
Assert.AreEqual(1, significant_proteins.Count);
Assert.AreEqual(1, gtn.Count);
Assert.AreEqual("1", gtn.First().Id);
Assert.AreEqual(0 - (decimal)Math.Log(2d / 3d, 2), gtn.First().log_odds_ratio);
List <ProteoformFamily> fams = SaveState.lollipop.getInterestingFamilies(gtn, families);
Assert.AreEqual(1, fams.Count);
Assert.AreEqual(1, fams[0].theoretical_proteoforms.Count);
}