public void MatePairMapper1() { IList <ISequence> sequences = new List <ISequence>(); IList <MatePair> pairedreads = new List <MatePair>(); Sequence seq = new Sequence(Alphabets.DNA, "GGGAAAAATCAGATT"); seq.ID = ">gi|263191773|ref|NG_015830.1| H**o sapiens insulin receptor" + "substrate 1 (IRS1) on chromosome 2.X1:2K"; sequences.Add(seq); seq = new Sequence(Alphabets.DNA, "ACGTGTGTGTCCCCC"); seq.ID = ">gi|263191773|ref|NG_015830.1| H**o sapiens insulin receptor" + "on chromosome 2.X1:10K"; sequences.Add(seq); seq = new Sequence(Alphabets.DNA, "ACGTGTGKAAAAAAA"); seq.ID = ">gi|263191773|ref | H**o sapiens ........insulin receptor" + "on chromosome 2.X1:50K"; sequences.Add(seq); seq = new Sequence(Alphabets.DNA, "GGGAAAAATCAGATT"); seq.ID = ">gi|263191773|ref|NG_015830.1| H**o sapiens insulin receptor" + "substrate 1 (IRS1) on chromosome 2.Y1:2K"; sequences.Add(seq); seq = new Sequence(Alphabets.DNA, "ACGTGTGTGTCCCCC"); seq.ID = ">gi|263191773|ref|NG_015830.1| H**o sapiens insulin receptor" + "on chromosome 2.Y1:10K"; sequences.Add(seq); seq = new Sequence(Alphabets.DNA, "ACGTGTGKAAAAAAA"); seq.ID = ">gi|263191773|ref | H**o sapiens ........insulin receptor" + "on chromosome 2.Y1:50K"; sequences.Add(seq); MatePairMapper pair = new MatePairMapper(); pairedreads = Sort(pair.Map(sequences), sequences); Assert.AreEqual(pairedreads.Count, 3); ISequence expectedForward = new Sequence(Alphabets.DNA, "ACGTGTGKAAAAAAA"); ISequence expectedReverse = new Sequence(Alphabets.DNA, "ACGTGTGKAAAAAAA"); string lib = "50K"; Assert.AreEqual(pairedreads[0].GetForwardRead(sequences).ToString(), expectedForward.ToString()); Assert.AreEqual(pairedreads[0].GetReverseRead(sequences).ToString(), expectedReverse.ToString()); Assert.AreEqual(pairedreads[0].Library, lib); Assert.AreEqual(pairedreads[0].MeanLengthOfLibrary, 65000); Assert.AreEqual(pairedreads[0].StandardDeviationOfLibrary, 13334); expectedForward = new Sequence(Alphabets.DNA, "ACGTGTGTGTCCCCC"); expectedReverse = new Sequence(Alphabets.DNA, "ACGTGTGTGTCCCCC"); lib = "10K"; Assert.AreEqual(pairedreads[1].GetForwardRead(sequences).ToString(), expectedForward.ToString()); Assert.AreEqual(pairedreads[1].GetReverseRead(sequences).ToString(), expectedReverse.ToString()); Assert.AreEqual(pairedreads[1].Library, lib); Assert.AreEqual(pairedreads[1].MeanLengthOfLibrary, 10000); Assert.AreEqual(pairedreads[1].StandardDeviationOfLibrary, 1000); expectedForward = new Sequence(Alphabets.DNA, "GGGAAAAATCAGATT"); expectedReverse = new Sequence(Alphabets.DNA, "GGGAAAAATCAGATT"); lib = "2K"; Assert.AreEqual(pairedreads[2].GetForwardRead(sequences).ToString(), expectedForward.ToString()); Assert.AreEqual(pairedreads[2].GetReverseRead(sequences).ToString(), expectedReverse.ToString()); Assert.AreEqual(pairedreads[2].Library, lib); Assert.AreEqual(pairedreads[2].MeanLengthOfLibrary, 2000); Assert.AreEqual(pairedreads[2].StandardDeviationOfLibrary, 100); }