private void xAxisTB_ChangeIndex(object sender, EventArgs e)
{
if (xAxisTB.Focused == false)
{
return;
}
//find selected image
TifFileInfo fi = null;
try
{
fi = IA.TabPages.TabCollections[IA.TabPages.SelectedIndex].tifFI;
}
catch { return; }
if (fi == null)
{
return;
}
if (fi.xAxisTB == xAxisTB.SelectedIndex)
{
return;
}
//Parse value
fi.delHist = true;
IA.delHist = true;
IA.UnDoBtn.Enabled = true;
IA.DeleteFromHistory();
AddXAxisTBToHistory(fi);
fi.xAxisTB = xAxisTB.SelectedIndex;
AddXAxisTBToHistory(fi);
//ReloadImage
IA.UpdateUndoBtns();
IA.MarkAsNotSaved();
IA.ReloadImages();
}
public OtsuSegmentation(Panel mainPanel, ImageAnalyser IA)
{
this.IA = IA;
//Core panel
panel.Dock = DockStyle.Top;
panel.BackColor = IA.FileBrowser.BackGround2Color1;
panel.ForeColor = IA.FileBrowser.ShriftColor1;
panel.Visible = false;
panel.Height = 150;
mainPanel.Controls.Add(panel);
panel.BringToFront();
#region Options
GroupBox optionGB = new GroupBox();
optionGB.Text = "Options:";
optionGB.BackColor = IA.FileBrowser.BackGround2Color1;
optionGB.ForeColor = IA.FileBrowser.ShriftColor1;
optionGB.Dock = DockStyle.Top;
optionGB.Height = 85;
panel.Controls.Add(optionGB);
optionGB.BringToFront();
Label Name = new Label();
Name.Text = "Thresholds:";
Name.Width = TextRenderer.MeasureText(Name.Text, Name.Font).Width;
Name.Location = new Point(5, 18);
optionGB.Controls.Add(Name);
Name.BringToFront();
ComboBox cb = thresholdsNumCB;
cb.Text = "0";
cb.Items.AddRange(new string[] { "0", "1", "2", "3", "4" });
cb.Width = 40;
cb.Location = new Point(80, 15);
optionGB.Controls.Add(cb);
cb.BringToFront();
cb.DropDownStyle = ComboBoxStyle.DropDownList;
cb.SelectedIndex = 0;
cb.AutoSize = false;
cb.SelectedIndexChanged += new EventHandler(delegate(object o, EventArgs e)
{
TifFileInfo fi = IA.TabPages.TabCollections[IA.TabPages.SelectedIndex].tifFI;
fi.thresholdsCBoxIndex[fi.cValue] = cb.SelectedIndex;
IA.ReloadImages();
});
CheckBox checkB = sumHistogramsCheckBox;
checkB.Text = "Use SUM histogram";
checkB.Tag = "Use SUM histogram:\nCalculates histograms for all images\nand merge them into one";
checkB.Width = 150;
checkB.Checked = true;
checkB.MouseHover += Control_MouseOver;
checkB.Location = new Point(7, 35);
optionGB.Controls.Add(checkB);
checkB.BringToFront();
checkB.CheckedChanged += new EventHandler(delegate(object o, EventArgs e)
{
TifFileInfo fi = IA.TabPages.TabCollections[IA.TabPages.SelectedIndex].tifFI;
fi.sumHistogramChecked[fi.cValue] = checkB.Checked;
if (((CheckBox)o).Focused == true)
{
IA.ReloadImages();
}
});
{
Button btn = ProcessBtn;
btn.Width = 115;
btn.FlatStyle = FlatStyle.Standard;
btn.BackColor = SystemColors.ButtonFace;
btn.ForeColor = Color.Black;
btn.Text = "Process";
btn.Location = new Point(5, 58);
optionGB.Controls.Add(btn);
btn.BringToFront();
btn.Click += new EventHandler(delegate(object o, EventArgs a)
{
int MLEVEL = thresholdsNumCB.SelectedIndex + 1;
if (IA.Segmentation.SegmentationCBox.SelectedIndex == 2)
{
IA.Segmentation.Kmeans.Start(MLEVEL);
return;
}
if (IA.Segmentation.SegmentationCBox.SelectedIndex != 1)
{
return;
}
TifFileInfo fi = IA.TabPages.TabCollections[IA.TabPages.SelectedIndex].tifFI;
if (fi.available == false)
{
MessageBox.Show("Image is not ready yet! \nTry again later.");
}
else
{
//background worker
var bgw = new BackgroundWorker();
bgw.WorkerReportsProgress = true;
bgw.DoWork += new DoWorkEventHandler(delegate(Object o1, DoWorkEventArgs a1)
{
try
{
//Segmentation event
run(fi, MLEVEL);
}
catch { }
//report progress
((BackgroundWorker)o1).ReportProgress(0);
});
bgw.ProgressChanged += new ProgressChangedEventHandler(delegate(Object o1, ProgressChangedEventArgs a1)
{
fi.available = true;
IA.FileBrowser.StatusLabel.Text = "Ready";
IA.MarkAsNotSaved();
IA.ReloadImages();
});
//Apply status
IA.FileBrowser.StatusLabel.Text = "Segmentation...";
fi.available = false;
//start bgw
bgw.RunWorkerAsync();
}
});
}
#endregion Options
#region Thresholds
GroupBox threshGB = new GroupBox();
threshGB.Text = "Thresholds:";
threshGB.BackColor = IA.FileBrowser.BackGround2Color1;
threshGB.ForeColor = IA.FileBrowser.ShriftColor1;
threshGB.Dock = DockStyle.Fill;
threshGB.Height = 50;
panel.Controls.Add(threshGB);
threshGB.BringToFront();
//Color btns
Panel colorPanel = new Panel();
colorPanel.Dock = DockStyle.Left;
colorPanel.Width = 25;
threshGB.Controls.Add(colorPanel);
Panel UpPanel = new Panel();
UpPanel.Dock = DockStyle.Top;
UpPanel.Height = 15;
threshGB.Controls.Add(UpPanel);
UpPanel.BringToFront();
for (int i = 0; i < colorBtns.Length; i++)
{
Button btn = new Button();
btn.FlatStyle = FlatStyle.Flat;
btn.FlatAppearance.BorderSize = 0;
btn.ForeColor = Color.Black;
btn.Text = "";
btn.Tag = i;
btn.Dock = DockStyle.Top;
btn.Height = 25;
colorPanel.Controls.Add(btn);
colorBtns[i] = btn;
btn.BringToFront();
btn.Visible = false;
btn.MouseDown += new MouseEventHandler(ColorBtn_Click);
btn.MouseHover += new EventHandler(delegate(object o, EventArgs a)
{
if (btn.Text == "")
{
TurnOnToolTip.SetToolTip(btn, "Color " + ((int)btn.Tag).ToString()
+ ":\nLeft click to Disable\nRight click to change color");
}
else
{
TurnOnToolTip.SetToolTip(btn, "Color " + ((int)btn.Tag).ToString()
+ " - Disabled\nLeft click to Enable");
}
});
}
//threshold track bars
for (int i = 0; i < threshTrackBars.Length; i++)
{
CTTrackBar tb = new CTTrackBar();
tb.Initialize();
tb.Panel.Dock = DockStyle.Top;
tb.BackColor(IA.FileBrowser.BackGround2Color1);
tb.ForeColor(IA.FileBrowser.ShriftColor1);
tb.Panel.Visible = false;
tb.Refresh(0, 0, 10);
tb.Name.Text = "T" + (i + 1).ToString();
tb.NamePanel.Width = 30;
threshGB.Controls.Add(tb.Panel);
threshTrackBars[i] = tb;
tb.Panel.BringToFront();
tb.Value.Changed += new ChangedValueEventHandler(delegate(Object o, ChangeValueEventArgs a)
{
TrackBar_ValueChange(a, tb);
});
}
#endregion Thresholds
}
private void TrackBar_ValueChange(ChangeValueEventArgs e, CTTrackBar tb)
{
//variables
TifFileInfo fi = IA.TabPages.TabCollections[IA.TabPages.SelectedIndex].tifFI;
int[] vals = fi.thresholdValues[fi.cValue];
int val = int.Parse(e.Value);
int valRef = val;
//find trackbar index
int index = 0;
for (int i = 0; i < threshTrackBars.Length; i++)
{
if (threshTrackBars[i] == tb)
{
index = i; break;
}
}
//Check the avaliable min
if (index > 0)
{
if (val <= vals[index])
{
val = vals[index] + 1;
}
}
//Check the avaliable max
if (index < fi.thresholds[fi.cValue] - 1)
{
if (val >= vals[index + 2])
{
val = vals[index + 2] - 1;
}
}
//refresh if value is wrong
if (val != valRef)
{
tb.Refresh(val, tb.TrackBar1.Minimum, tb.TrackBar1.Maximum);
}
//apply changes
if (val != vals[index + 1])
{
if (tb.TrackBar1.Focused == true | tb.TextBox1.Focused == true |
tb.ApplyBtn.Focused == true)
{
#region apply to history
fi.delHist = true;
IA.delHist = true;
IA.UnDoBtn.Enabled = true;
IA.DeleteFromHistory();
fi.History.Add("segmentation.SetThreshOld("
+ fi.cValue + "," + (index + 1).ToString() + ","
+ vals[index + 1].ToString() + ")");
fi.History.Add("segmentation.SetThreshOld("
+ fi.cValue + "," + (index + 1).ToString() + ","
+ val.ToString() + ")");
IA.UpdateUndoBtns();
IA.MarkAsNotSaved();
#endregion apply to history
}
vals[index + 1] = val;
IA.ReloadImages();
}
}
private void createDialog()
{
Dialog.FormBorderStyle = FormBorderStyle.FixedDialog;
Dialog.Text = "Watershed";
Dialog.StartPosition = FormStartPosition.CenterScreen;
Dialog.WindowState = FormWindowState.Normal;
Dialog.MinimizeBox = false;
Dialog.MaximizeBox = false;
Dialog.BackColor = IA.FileBrowser.BackGround2Color1;
Dialog.ForeColor = IA.FileBrowser.ShriftColor1;
Dialog.FormClosing += new FormClosingEventHandler(delegate(object o, FormClosingEventArgs a)
{
Dialog.Visible = false;
a.Cancel = true;
});
Dialog.Width = 240;
Dialog.Height = 190;
Dialog.SuspendLayout();
/*
* Label ThresholdLab = new Label();
* ThresholdLab.Text = "Select threshold:";
* ThresholdLab.Width = 100;
* ThresholdLab.Location = new System.Drawing.Point(10,15);
* Dialog.Controls.Add(ThresholdLab);
*
* ThresholdCB.Width = 30;
* ThresholdCB.Location = new System.Drawing.Point(110, 13);
* Dialog.Controls.Add(ThresholdCB);
* ThresholdCB.SelectedIndexChanged += new EventHandler(delegate (object o, EventArgs a)
* {
* threshold = ThresholdCB.SelectedIndex + 1;
* });
*/
Label ToleranceLab = new Label();
ToleranceLab.Text = "Tolerance:";
ToleranceLab.Width = 100;
ToleranceLab.Location = new System.Drawing.Point(10, 15);
Dialog.Controls.Add(ToleranceLab);
ToleranceTB.Text = tolerance.ToString();
ToleranceTB.Width = 30;
ToleranceTB.Location = new System.Drawing.Point(110, 13);
Dialog.Controls.Add(ToleranceTB);
Label DistanceLab = new Label();
DistanceLab.Text = "Distance Transform:";
DistanceLab.Width = 100;
DistanceLab.Location = new System.Drawing.Point(10, 45);
Dialog.Controls.Add(DistanceLab);
ComboBox DistanceCB = new ComboBox();
DistanceCB.Width = 110;
DistanceCB.Items.AddRange(new string[] { "Euclidean", "SquaredEuclidean", "Chessboard", "Manhattan" });
DistanceCB.SelectedIndex = 0;
DistanceCB.SelectedIndexChanged += new EventHandler(delegate(object o, EventArgs a)
{
switch (DistanceCB.SelectedIndex)
{
case 0:
distance = DistanceTransformMethod.Euclidean;
break;
case 1:
distance = DistanceTransformMethod.SquaredEuclidean;
break;
case 2:
distance = DistanceTransformMethod.Chessboard;
break;
case 3:
distance = DistanceTransformMethod.Manhattan;
break;
default:
distance = DistanceTransformMethod.Euclidean;
break;
}
});
DistanceCB.Location = new System.Drawing.Point(110, 43);
Dialog.Controls.Add(DistanceCB);
FillHolesCB.Text = "Fill holes";
FillHolesCB.Location = new System.Drawing.Point(15, 75);
Dialog.Controls.Add(FillHolesCB);
//buttons
Panel okBox = new Panel();
okBox.Height = 40;
okBox.Dock = DockStyle.Bottom;
Dialog.Controls.Add(okBox);
Button okBtn = new Button();
okBtn.Text = "Process";
okBtn.BackColor = System.Drawing.SystemColors.ButtonFace;
okBtn.ForeColor = System.Drawing.Color.Black;
okBtn.Location = new System.Drawing.Point(20, 10);
okBtn.Anchor = AnchorStyles.Top | AnchorStyles.Left;
okBox.Controls.Add(okBtn);
Button cancelBtn = new Button();
cancelBtn.Text = "Cancel";
cancelBtn.BackColor = System.Drawing.SystemColors.ButtonFace;
cancelBtn.Location = new System.Drawing.Point(Dialog.Width - cancelBtn.Width - 60, 10);
cancelBtn.ForeColor = System.Drawing.Color.Black;
cancelBtn.Anchor = AnchorStyles.Top | AnchorStyles.Right;
okBox.Controls.Add(cancelBtn);
okBtn.Click += new EventHandler(delegate(object sender, EventArgs e)
{
try
{
tolerance = float.Parse(ToleranceTB.Text);
}
catch
{
MessageBox.Show("Tolerance must be numeric!");
return;
}
Dialog.Visible = false;
//event
TifFileInfo fi = null;
try
{
fi = IA.TabPages.TabCollections[IA.TabPages.SelectedIndex].tifFI;
}
catch { return; }
if (fi == null)
{
return;
}
if (!fi.available)
{
MessageBox.Show("Image is not ready yet! \nTry again later.");
return;
}
int C = fi.cValue;
//background worker
var bgw = new BackgroundWorker();
bgw.WorkerReportsProgress = true;
fi.available = false;
fi.fillHoles = FillHolesCB.Checked;
//Add event for projection here
bgw.DoWork += new DoWorkEventHandler(delegate(Object o, DoWorkEventArgs a)
{
ProcessFilter(fi, C, this.tolerance, 1, this.distance);
((BackgroundWorker)o).ReportProgress(0);
});
bgw.ProgressChanged += new ProgressChangedEventHandler(delegate(Object o, ProgressChangedEventArgs a)
{
if (a.ProgressPercentage == 0)
{
IA.Segmentation.MyFilters.addToHistoryOldInfo(C, fi);
fi.newFilterHistory[C].Add(
ToString(fi, C, this.tolerance, 1, this.distance));
IA.Segmentation.MyFilters.addToHistoryNewInfo(C, fi);
IA.MarkAsNotSaved();
IA.ReloadImages();
}
fi.available = true;
IA.FileBrowser.StatusLabel.Text = "Ready";
});
//Start background worker
IA.FileBrowser.StatusLabel.Text = "Watershed...";
//start bgw
bgw.RunWorkerAsync();
});
cancelBtn.Click += new EventHandler(delegate(object sender, EventArgs e)
{
Dialog.Visible = false;
});
Dialog.KeyPreview = true;
Dialog.KeyDown += new KeyEventHandler(delegate(object sender, KeyEventArgs e)
{
switch (e.KeyCode)
{
case Keys.Escape:
Dialog.Visible = false;
break;
case Keys.Enter:
okBtn.PerformClick();
break;
default:
break;
}
});
Dialog.ResumeLayout();
}
private void ColBtn_Click(object sender, MouseEventArgs e)
{
TifFileInfo fi = IA.TabPages.TabCollections[IA.TabPages.SelectedIndex].tifFI;
Button btn = (Button)sender;
if (e.Button == MouseButtons.Left)
{
//disable/enable color
if (fi.SpotColor[fi.cValue] == Color.Transparent)
{
fi.SpotColor[fi.cValue] = fi.RefSpotColor[fi.cValue];
}
else
{
fi.SpotColor[fi.cValue] = Color.Transparent;
}
#region apply to history
//segmentation.SpotDetector(chanelN,ThreshVal,HTML color, tail type, thresh type)
Color val = fi.RefSpotColor[fi.cValue];
Color oldVal = Color.Transparent;
if (fi.SpotColor[fi.cValue] == Color.Transparent)
{
val = Color.Transparent; oldVal = fi.RefSpotColor[fi.cValue];
}
fi.delHist = true;
IA.delHist = true;
IA.UnDoBtn.Enabled = true;
IA.DeleteFromHistory();
fi.History.Add("segmentation.SpotDetector("
+ fi.cValue.ToString() + "," +
fi.SpotThresh[fi.cValue].ToString() + "," +
fi.spotSensitivity[fi.cValue].ToString() + "," +
ColorTranslator.ToHtml(oldVal) + "," +
fi.SpotTailType[fi.cValue] + "," +
fi.SelectedSpotThresh[fi.cValue].ToString() + "," +
fi.typeSpotThresh[fi.cValue].ToString() + ")");
fi.History.Add("segmentation.SpotDetector("
+ fi.cValue.ToString() + "," +
fi.SpotThresh[fi.cValue].ToString() + "," +
fi.spotSensitivity[fi.cValue].ToString() + "," +
ColorTranslator.ToHtml(val) + "," +
fi.SpotTailType[fi.cValue] + "," +
fi.SelectedSpotThresh[fi.cValue].ToString() + "," +
fi.typeSpotThresh[fi.cValue].ToString() + ")");
IA.UpdateUndoBtns();
IA.MarkAsNotSaved();
#endregion apply to history
IA.ReloadImages();
}
else if (e.Button == MouseButtons.Right & btn.Text == "")
{
//change color
ColorDialog colorDialog1 = new ColorDialog();
colorDialog1.AllowFullOpen = true;
colorDialog1.AnyColor = true;
colorDialog1.FullOpen = true;
colorDialog1.Color = fi.RefSpotColor[fi.cValue];
//set Custom Colors
if (IA.settings.CustomColors[IA.FileBrowser.ActiveAccountIndex] != "@")
{
List <int> colorsList = new List <int>();
foreach (string j in IA.settings.CustomColors[IA.FileBrowser.ActiveAccountIndex]
.Split(new[] { "\t" }, StringSplitOptions.None))
{
colorsList.Add(int.Parse(j));
}
colorDialog1.CustomColors = colorsList.ToArray();
}
// Show the color dialog.
DialogResult result = colorDialog1.ShowDialog();
//Copy Custom Colors
int[] colors = (int[])colorDialog1.CustomColors.Clone();
string txt = "@";
if (colors.Length > 0)
{
txt = colors[0].ToString();
for (int j = 1; j < colors.Length; j++)
{
txt += "\t" + colors[j].ToString();
}
}
IA.settings.CustomColors[IA.FileBrowser.ActiveAccountIndex] = txt;
IA.settings.Save();
if (result == DialogResult.OK)
{
#region apply to history
//segmentation.SpotDetector(chanelN,ThreshVal,HTML color, tail type, thresh type)
fi.delHist = true;
IA.delHist = true;
IA.UnDoBtn.Enabled = true;
IA.DeleteFromHistory();
fi.History.Add("segmentation.SpotDetector("
+ fi.cValue.ToString() + "," +
fi.SpotThresh[fi.cValue].ToString() + "," +
fi.spotSensitivity[fi.cValue].ToString() + "," +
ColorTranslator.ToHtml(fi.RefSpotColor[fi.cValue]) + "," +
fi.SpotTailType[fi.cValue] + "," +
fi.SelectedSpotThresh[fi.cValue].ToString() + "," +
fi.typeSpotThresh[fi.cValue].ToString() + ")");
fi.History.Add("segmentation.SpotDetector("
+ fi.cValue.ToString() + "," +
fi.SpotThresh[fi.cValue].ToString() + "," +
fi.spotSensitivity[fi.cValue].ToString() + "," +
ColorTranslator.ToHtml(colorDialog1.Color) + "," +
fi.SpotTailType[fi.cValue] + "," +
fi.SelectedSpotThresh[fi.cValue].ToString() + "," +
fi.typeSpotThresh[fi.cValue].ToString() + ")");
IA.UpdateUndoBtns();
IA.MarkAsNotSaved();
#endregion apply to history
fi.RefSpotColor[fi.cValue] = colorDialog1.Color;
fi.SpotColor[fi.cValue] = colorDialog1.Color;
IA.ReloadImages();
}
}
}
