public override void Update(object sender, UpdateQuantificationItemEventArgs e) { IQuantificationSummaryOption option = e.Option as IQuantificationSummaryOption; IIdentifiedResult mr = e.Item as IIdentifiedResult; string xTitle = MyConvert.Format("(Log({0}) + Log({1})) / 2", option.Func.ReferenceKey, option.Func.SampleKey); string yTitle = MyConvert.Format("Log(Ratio)"); panel.InitGraphPane(title, xTitle, yTitle, true, 0.0); try { HashSet <IIdentifiedSpectrum> spectra = new HashSet <IIdentifiedSpectrum>(); var groups = from g in mr where g[0].IsEnabled(true) && option.IsProteinRatioValid(g[0]) select g; foreach (var mpg in groups) { var peptides = from p in mpg.GetPeptides() where p.IsEnabled(true) && option.IsPeptideRatioValid(p) select p; spectra.UnionWith(peptides); } List <LSPADItem> items = new List <LSPADItem>(); foreach (var pep in spectra) { double refIntensity = Math.Log(option.Func.GetReferenceIntensity(pep)); double sampleIntensity = Math.Log(option.Func.GetSampleIntensity(pep)); double A = (refIntensity + sampleIntensity) / 2; double ratio = Math.Log(option.GetPeptideRatio(pep)); items.Add(new LSPADItem() { LogRatio = ratio, Intensity = A, Tag = pep }); } LSPADItem.CalculatePValue(items); //this.panel.DrawProbabilityRange(maxX, ratios); //this.panel.AddPoints(pplSelected, SelectedColor, "Current Peptide"); //this.panel.AddPoints(pplGroup, GroupColor, "Current Protein"); //this.panel.AddPoints(pplOutlier, OutlierColor, "Outlier"); //this.panel.AddPoints(pplNormal, NormalColor, "Other"); } finally { ZedGraphicExtension.UpdateGraph(this.zgcGraph); } }
public override void Update(object sender, UpdateQuantificationItemEventArgs e) { IQuantificationSummaryOption option = e.Option as IQuantificationSummaryOption; IIdentifiedResult mr = e.Item as IIdentifiedResult; string xTitle = MyConvert.Format("(Log({0}) + Log({1})) / 2", option.Func.ReferenceKey, option.Func.SampleKey); string yTitle = MyConvert.Format("Log(Ratio)"); panel.InitGraphPane(title, xTitle, yTitle, true, 0.0); try { var pplNormal = new PointPairList(); var pplSelected = new PointPairList(); var pplOutlier = new PointPairList(); var pplGroup = new PointPairList(); var groups = from g in mr where g[0].IsEnabled(true) && option.IsProteinRatioValid(g[0]) select g; HashSet <IIdentifiedSpectrum> spectra = new HashSet <IIdentifiedSpectrum>(); double maxX = 0.0; PointPairList ppl; foreach (var mpg in groups) { var peptides = from p in mpg.GetPeptides() where p.IsEnabled(true) && option.IsPeptideRatioValid(p) select p; spectra.UnionWith(peptides); foreach (var pep in peptides) { if (pep.Selected) { ppl = pplSelected; } else if (mpg.Selected) { ppl = pplGroup; } else if (option.IsPeptideOutlier(pep)) { ppl = pplOutlier; } else { ppl = pplNormal; } double refIntensity = Math.Log(option.Func.GetReferenceIntensity(pep)); double sampleIntensity = Math.Log(option.Func.GetSampleIntensity(pep)); double A = (refIntensity + sampleIntensity) / 2; double ratio = Math.Log(option.GetPeptideRatio(pep)); ppl.Add(A, ratio); ppl[ppl.Count - 1].Tag = new Pair <IIdentifiedProteinGroup, IIdentifiedSpectrum>(mpg, pep); maxX = Math.Max(A, maxX); } } this.panel.ClearData(); var ratios = (from pep in spectra let ratio = Math.Log(option.GetPeptideRatio(pep)) orderby ratio select ratio).ToList(); this.panel.DrawProbabilityRange(maxX, ratios); this.panel.AddPoints(pplSelected, SelectedColor, "Current Peptide"); this.panel.AddPoints(pplGroup, GroupColor, "Current Protein"); this.panel.AddPoints(pplOutlier, OutlierColor, "Outlier"); this.panel.AddPoints(pplNormal, NormalColor, "Other"); } finally { ZedGraphicExtension.UpdateGraph(this.zgcGraph); } }