private void BelaGraphPeptideDistribution_SizeChanged(object sender, SizeChangedEventArgs e)
{
BelaGraphPeptideDistribution.Plot(BelaGraph.BackgroundOption.YellowXGradient, true, true, new System.Drawing.Font("Courier New", 14));
}
private void PlotQuants()
{
//------------------------------------
BelaGraphPeptideDistribution.ClearDataBuffer();
BelaGraphPeptideDistribution.XLabel = "-log(p-value)";
BelaGraphPeptideDistribution.YLabel = "log(fold)";
BelaGraphPeptideDistribution.XRound = 4;
BelaGraphPeptideDistribution.YRound = 4;
SolidColorBrush redBrush = Brushes.Red.Clone();
redBrush.Opacity = 0.5;
SolidColorBrush greenBrush = Brushes.Green.Clone();
greenBrush.Opacity = 0.5;
SolidColorBrush grayBrush = Brushes.LightGray.Clone();
grayBrush.Opacity = 0.4;
BelaGraph.DataVector greenVectorPep = new BelaGraph.DataVector(BelaGraph.GraphStyle.Bubble, "Green dots", greenBrush);
BelaGraph.DataVector redVectorPep = new BelaGraph.DataVector(BelaGraph.GraphStyle.Bubble, "Red dots", redBrush);
BelaGraph.DataVector grayVectorPep = new BelaGraph.DataVector(BelaGraph.GraphStyle.Bubble, "Gray dots", grayBrush);
foreach (PepQuant pq in MyPeptides)
{
//Skip Quants composed mainly of zeros or quants that have exactly 0.5 as a p-value
if (pq.TTest == 0.5)
{
continue;
}
double avgLogFold = pq.AVGLogFold;
double pValue = pq.TTest;
if (avgLogFold < -3)
{
avgLogFold = -3;
}
if (avgLogFold > 3)
{
avgLogFold = 3;
}
BelaGraph.PointCav dp = new BelaGraph.PointCav();
dp.Y = avgLogFold;
dp.X = Math.Round(1 - pValue, 4);
dp.ExtraParam = dp.Y.ToString();
dp.MouseOverTip = "Sequence: " + pq.Sequence + "\nQuants: " + pq.MyQuants.Count + "\nAvg Log Fold: " + avgLogFold + "\nTtest: " + pValue;
if (myPeptidesTMP.Exists(a => a.Sequence.Equals(pq.Sequence)))
{
if (dp.Y > 0)
{
greenVectorPep.AddPoint(dp);
}
else
{
redVectorPep.AddPoint(dp);
}
}
else
{
if ((bool)CheckBoxPlotFilteredPeptides.IsChecked)
{
grayVectorPep.AddPoint(dp);
}
}
}
grayVectorPep.ThePoints.Sort((a, b) => b.X.CompareTo(a.X));
redVectorPep.ThePoints.Sort((a, b) => b.X.CompareTo(a.X));
greenVectorPep.ThePoints.Sort((a, b) => b.X.CompareTo(a.X));
BelaGraphPeptideDistribution.AddDataVector(grayVectorPep);
BelaGraphPeptideDistribution.AddDataVector(redVectorPep);
BelaGraphPeptideDistribution.AddDataVector(greenVectorPep);
BelaGraphPeptideDistribution.Plot(BelaGraph.BackgroundOption.YellowXGradient, true, true, new System.Drawing.Font("Courier New", 14));
}
