public KnownIonSets(IonTable ionTable)
{
ionSets = new List <KnownIonSet>();
if (ionTable is null)
{
ionTable = new IonTable();
}
ionSets.Add(new KnownIonSet(
name: "JLJP screenshot",
details: "Results frrom screenshot on SourceForge: https://a.fsdn.com/con/app/proj/jljp/screenshots/GUI.png/max/max/1.jpg",
expectedLjp_mV: -20.79558643,
temperatureC: 25,
expectedAccuracy_mV: .5,
ions: new List <Ion>()
{
new Ion("Zn", 9, 2.84E-2),
new Ion("K", 0, 3),
new Ion("Cl", 18, 3.062)
},
ionTable: ionTable));
ionSets.Add(new KnownIonSet(
name: "Ng and Barry (1994)",
details: "Table 2 of: https://doi.org/10.1016/0165-0270(94)00087-W",
expectedLjp_mV: -8.2,
temperatureC: 25,
expectedAccuracy_mV: .5,
ions: new List <Ion>()
{
new Ion("Ca", 50, 0),
new Ion("Cl", 200, 100),
new Ion("Mg", 50, 0),
new Ion("Li", 0, 100)
},
ionTable: ionTable));
ionSets.Add(new KnownIonSet(
name: "JPCalcWin manual page 7",
details: "JPCalcWin manual (page 7): https://medicalsciences.med.unsw.edu.au/sites/default/files/soms/page/ElectroPhysSW/JPCalcWin-Demo%20Manual.pdf",
expectedLjp_mV: +8.74,
temperatureC: 20,
expectedAccuracy_mV: 0.5,
ions: new List <Ion>()
{
new Ion("Na", 10, 145),
new Ion("Cl", 10, 145),
new Ion("Cs", 135, 0),
new Ion("F", 135, 0)
},
ionTable: ionTable));
/* Our standard solution contains the following (in mM):
* 110 K-gluconate
* 10 N-[2-Hydroxyethyl]piperazine-N′-[2-ethanesulfonic acid] (HEPES)
* 1.0 ethyleneglycotetraacetic acid (EGTA)
* 20 KCl
* 2.0 MgCl2
* 2.0 Na2ATP
* 0.25 Na3GTP
* 10 phosphocreatine (di-tris)
* pH 7.3, 290 mOsm, add 0.5% biocytin
*/
ionSets.Add(new KnownIonSet(
name: "Patch Clamp Solutions (K-glu)",
details: "Patch-Clamp Analysis (Watz and Wolfgang, 2007) Second Edition page 191: http://springer.com/gp/book/9781588297051",
expectedLjp_mV: +15.729,
temperatureC: 25,
expectedAccuracy_mV: .5,
ions: new List <Ion>()
{
new Ion("Ca", 0, 2.4),
new Ion("Cl", 2, 133.8),
new Ion("Gluconate", 125, 0),
new Ion("H2PO4", 0, 1.2),
new Ion("HCO3", 0, 25),
new Ion("HEPES", 10, 0),
new Ion("K", 125, 3),
new Ion("Mg", 1, 1.5),
new Ion("SO4", 0, 1.5),
new Ion("Na", 4.25, 152.2)
},
ionTable: ionTable));
// increase KCl by 30 mM (20 -> 50) and decrease Kglu by 30 (110 -> 80)
// [K] doesn't change, but Cl rises by 30 and Glu drops by 30
ionSets.Add(new KnownIonSet(
name: "Patch Clamp Solutions (K-glu, high [Cl])",
details: "Modified from Watz and Wolfgang (2007) page 191: http://springer.com/gp/book/9781588297051",
expectedLjp_mV: +12.441,
temperatureC: 25,
expectedAccuracy_mV: .5,
ions: new List <Ion>()
{
new Ion("Ca", 0, 2.4),
new Ion("Cl", 2 + 30, 133.8),
new Ion("Gluconate", 125 - 30, 0),
new Ion("H2PO4", 0, 1.2),
new Ion("HCO3", 0, 25),
new Ion("HEPES", 10, 0),
new Ion("K", 125, 3),
new Ion("Mg", 1, 1.5),
new Ion("SO4", 0, 1.5),
new Ion("Na", 4.25, 152.2)
},
ionTable: ionTable));
}
public static void Test()
{
var table = new IonTable();
Debug.WriteLine(table);
}
public KnownIonSet(string name, string details, double expectedLjp_mV, double expectedAccuracy_mV, List <Ion> ions, double temperatureC, IonTable ionTable)
{
this.name = name;
this.details = details;
this.expectedLjp_mV = expectedLjp_mV;
this.expectedAccuracy_mV = expectedAccuracy_mV;
this.ions = ionTable.Lookup(ions);
this.temperatureC = temperatureC;
}