private void generateDRCsToolStripMenuItem_Click(object sender, EventArgs e)
{
int nbrofdrugs = 6;
int nbrofconcentrations = 10;
int nbrofdesc = 3;
double[, ,] DRC = new double[nbrofconcentrations, nbrofdrugs, nbrofdesc];
float[] X1 = new float[nbrofconcentrations];
X1[0] = 1000;
for (int i = 1; i < X1.Length; i++)
{
X1[i] = X1[i - 1] / 3;
}
Random Bot = new Random();
Random To = new Random();
Random Ec5 = new Random();
Random Slop = new Random();
CompleteScreening = new cScreening("Current Screen", this.GlobalInfo);
CompleteScreening.Columns = nbrofconcentrations;
CompleteScreening.Rows = nbrofdrugs;
for (int j = 0; j < nbrofdrugs; j++)
{
for (int k = 0; k < nbrofdesc; k++)
{
float Bottom = Bot.Next(10);
float Top = To.Next(80, 100);
float Ec50 = Ec5.Next(40, 60);
float Slope = Slop.Next(1, 5);
for (int i = 0; i < X1.Length; i++)
{
//DRC[i, j, k] = Bottom + (Top - Bottom) / (1 + Math.Pow(((Math.Pow(10, Ec50) / Math.Pow(10, X1[0]))), Slope));
DRC[i, j, k] = Bottom + (Top - Bottom) / (1 + Math.Pow((X1[i] / Ec50), -Slope));
}
}
}
cPlate NewPlate = new cPlate("Cpds", "Plate0", CompleteScreening);
CompleteScreening.AddPlate(NewPlate);
CompleteScreening.ListDescriptors.Clean();
for (int k = 0; k < nbrofdesc; k++)
{
cDescriptorsType DescType = new cDescriptorsType("Desc" + k, true, 1, GlobalInfo);
CompleteScreening.ListDescriptors.AddNew(DescType);
}
for (int j = 0; j < nbrofdrugs; j++)
{
for (int i = 0; i < nbrofconcentrations; i++)
{
List<cDescriptor> LDesc = new List<cDescriptor>();
for (int k = 0; k < nbrofdesc; k++)
{
//DRC[i, j, k] = Bottom + (Top - Bottom) / (1 + Math.Pow(((Math.Pow(10, Ec50) / Math.Pow(10, X1[0]))), Slope));
cDescriptor CurrentDesc = new cDescriptor(DRC[i, j, k], CompleteScreening.ListDescriptors[k], CompleteScreening);
LDesc.Add(CurrentDesc);
}
cWell NewWell = new cWell(LDesc, i+1, j+1, CompleteScreening, NewPlate);
NewWell.Concentration = X1[i];
NewPlate.AddWell(NewWell);
}
}
CompleteScreening.ListDescriptors.UpDateDisplay();
CompleteScreening.UpDatePlateListWithFullAvailablePlate();
for (int idxP = 0; idxP < CompleteScreening.ListPlatesActive.Count; idxP++)
CompleteScreening.ListPlatesActive[idxP].UpDataMinMax();
StartingUpDateUI();
this.toolStripcomboBoxPlateList.Items.Clear();
for (int IdxPlate = 0; IdxPlate < CompleteScreening.ListPlatesActive.Count; IdxPlate++)
{
string Name = CompleteScreening.ListPlatesActive.GetPlate(IdxPlate).Name;
this.toolStripcomboBoxPlateList.Items.Add(Name);
PlateListWindow.listBoxPlateNameToProcess.Items.Add(Name);
PlateListWindow.listBoxAvaliableListPlates.Items.Add(Name);
}
CompleteScreening.CurrentDisplayPlateIdx = 0;
CompleteScreening.SetSelectionType(comboBoxClass.SelectedIndex - 1);
UpdateUIAfterLoading();
}
private void hitsDistributionMapToolStripMenuItem_Click(object sender, EventArgs e)
{
if (cGlobalInfo.CurrentScreening == null) return;
List<cPanelForDisplayArray> ListPlates = new List<cPanelForDisplayArray>();
foreach (cPlate CurrentPlate in cGlobalInfo.CurrentScreening.ListPlatesActive)
{
ListPlates.Add(new FormToDisplayPlate(CurrentPlate));
}
cWindowToDisplayEntireScreening WindowToDisplayArray = new cWindowToDisplayEntireScreening(ListPlates, cGlobalInfo.CurrentScreening.ListDescriptors[cGlobalInfo.CurrentScreening.ListDescriptors.CurrentSelectedDescriptorIdx].GetName(), 6);
WindowToDisplayArray.checkBoxDisplayClasses.Checked = true;
WindowToDisplayArray.Text = "Generate Hits Distribution Maps";
WindowToDisplayArray.Show();
System.Windows.Forms.DialogResult ResWin = MessageBox.Show("By applying this process, the current screening will be entirely updated ! Proceed ?", "Warning", MessageBoxButtons.YesNo, MessageBoxIcon.Warning);
if (ResWin == System.Windows.Forms.DialogResult.No)
{
WindowToDisplayArray.Close();
return;
}
WindowToDisplayArray.Close();
if (cGlobalInfo.CurrentScreening != null) cGlobalInfo.CurrentScreening.Close3DView();
// CompleteScreening.ListDescriptors.RemoveDesc(CompleteScreening.ListDescriptors[IntToTransfer], CompleteScreening);
cScreening MergedScreening = new cScreening("Class Screen");
MergedScreening.PanelForPlate = this.panelForPlate;
MergedScreening.Rows = cGlobalInfo.CurrentScreening.Rows;
MergedScreening.Columns = cGlobalInfo.CurrentScreening.Columns;
MergedScreening.ListPlatesAvailable = new cListPlates();
// create the descriptor
MergedScreening.ListDescriptors.Clean();
List<cDescriptorType> ListDescType = new List<cDescriptorType>();
List<int[][]> Values = new List<int[][]>();
for (int i = 0; i < cGlobalInfo.ListWellClasses.Count; i++)
{
cDescriptorType DescClass = new cDescriptorType("Class_" + i, true, 1);
ListDescType.Add(DescClass);
MergedScreening.ListDescriptors.AddNew(DescClass);
int[][] TMpVal = new int[MergedScreening.Columns][];
for (int ii = 0; ii < MergedScreening.Columns; ii++)
TMpVal[ii] = new int[MergedScreening.Rows];
Values.Add(TMpVal);
}
MergedScreening.ListDescriptors.CurrentSelectedDescriptorIdx = 0;
foreach (cPlate CurrentPlate in cGlobalInfo.CurrentScreening.ListPlatesActive)
{
foreach (cWell TmpWell in CurrentPlate.ListActiveWells)
{
int Class = TmpWell.GetCurrentClassIdx();
if (Class >= 0)
Values[Class][TmpWell.GetPosX() - 1][TmpWell.GetPosY() - 1]++;
}
}
cPlate NewPlate = new cPlate(cGlobalInfo.CurrentScreening.GetName(), MergedScreening);
for (int X = 0; X < cGlobalInfo.CurrentScreening.Columns; X++)
for (int Y = 0; Y < cGlobalInfo.CurrentScreening.Rows; Y++)
{
cListSignature LDesc = new cListSignature();
for (int i = 0; i < cGlobalInfo.ListWellClasses.Count; i++)
{
cSignature Desc = new cSignature(Values[i][X][Y], ListDescType[i], cGlobalInfo.CurrentScreening);
LDesc.Add(Desc);
}
cWell NewWell = new cWell(LDesc, X + 1, Y + 1, MergedScreening, NewPlate);
// NewWell.SetCpdName("Well [" + (X + 1) + ":" + (Y + 1) + "]");
NewPlate.AddWell(NewWell);
}
// check if the plate exist already
MergedScreening.AddPlate(NewPlate);
MergedScreening.ListPlatesActive = new cListPlates();
cGlobalInfo.WindowHCSAnalyzer.toolStripcomboBoxPlateList.Items.Clear();
for (int i = 0; i < MergedScreening.ListPlatesAvailable.Count; i++)
{
MergedScreening.ListPlatesActive.Add(MergedScreening.ListPlatesAvailable[i]);
cGlobalInfo.WindowHCSAnalyzer.toolStripcomboBoxPlateList.Items.Add(NewPlate.GetName());
}
cGlobalInfo.CurrentScreening.ListDescriptors = MergedScreening.ListDescriptors;
cGlobalInfo.CurrentScreening.ListPlatesAvailable = MergedScreening.ListPlatesAvailable;
cGlobalInfo.CurrentScreening.ListPlatesActive = MergedScreening.ListPlatesActive;
cGlobalInfo.CurrentScreening.UpDatePlateListWithFullAvailablePlate();
for (int idxP = 0; idxP < cGlobalInfo.CurrentScreening.ListPlatesActive.Count; idxP++)
cGlobalInfo.CurrentScreening.ListPlatesActive[idxP].UpDataMinMax();
cGlobalInfo.CurrentScreening.CurrentDisplayPlateIdx = 0;
cGlobalInfo.CurrentScreening.GetCurrentDisplayPlate().DisplayDistribution(cGlobalInfo.CurrentScreening.ListDescriptors.GetActiveDescriptor(), true);
ListPlates = new List<cPanelForDisplayArray>();
for (int DescIdx = 0; DescIdx < cGlobalInfo.CurrentScreening.ListDescriptors.Count; DescIdx++)
{
if (cGlobalInfo.CurrentScreening.ListDescriptors[DescIdx].IsActive())
ListPlates.Add(new FormToDisplayDescriptorPlate(cGlobalInfo.CurrentScreening.GetCurrentDisplayPlate(), cGlobalInfo.CurrentScreening, DescIdx));
}
cWindowToDisplayEntireDescriptors WindowToDisplayDesc = new cWindowToDisplayEntireDescriptors(ListPlates, cGlobalInfo.CurrentScreening.GetCurrentDisplayPlate().GetName(), cGlobalInfo.ListWellClasses.Count);
WindowToDisplayDesc.checkBoxGlobalNormalization.Checked = true;
WindowToDisplayDesc.Show();
}
private void convertDRCToWellToolStripMenuItem1_Click(object sender, EventArgs e)
{
System.Windows.Forms.DialogResult ResWin = MessageBox.Show("By applying this process, the current screening will be entirely updated ! Proceed ?", "Warning", MessageBoxButtons.YesNo, MessageBoxIcon.Warning);
if (ResWin == System.Windows.Forms.DialogResult.No) return;
//foreach (cDescriptorsType DescType in CompleteScreening.ListDescriptors)
//{
// CompleteScreening.ListDescriptors.RemoveDescUnSafe(DescType, CompleteScreening);
//}
if (CompleteScreening != null) CompleteScreening.Close3DView();
// CompleteScreening.ListDescriptors.RemoveDesc(CompleteScreening.ListDescriptors[IntToTransfer], CompleteScreening);
cScreening MergedScreening = new cScreening("Merged Screen", GlobalInfo);
MergedScreening.PanelForPlate = this.panelForPlate;
MergedScreening.Rows = CompleteScreening.Rows;
MergedScreening.Columns = CompleteScreening.Columns;
MergedScreening.ListPlatesAvailable = new cExtendPlateList();
// create the descriptor
MergedScreening.ListDescriptors.Clean();
int Idesc = 0;
List<cDescriptorsType> ListDescType = new List<cDescriptorsType>();
for (int i = 0; i < CompleteScreening.ListDescriptors.Count; i++)
{
if (!CompleteScreening.ListDescriptors[i].IsActive()) continue;
cDescriptorsType DescEC50 = new cDescriptorsType("EC50_" + CompleteScreening.ListDescriptors[i].GetName(), true, 1, GlobalInfo);
ListDescType.Add(DescEC50);
MergedScreening.ListDescriptors.AddNew(DescEC50);
cDescriptorsType DescTop = new cDescriptorsType("Top_" + CompleteScreening.ListDescriptors[i].GetName(), true, 1, GlobalInfo);
ListDescType.Add(DescTop);
MergedScreening.ListDescriptors.AddNew(DescTop);
cDescriptorsType DescBottom = new cDescriptorsType("Bottom_" + CompleteScreening.ListDescriptors[i].GetName(), true, 1, GlobalInfo);
ListDescType.Add(DescBottom);
MergedScreening.ListDescriptors.AddNew(DescBottom);
cDescriptorsType DescSlope = new cDescriptorsType("Slope_" + CompleteScreening.ListDescriptors[i].GetName(), true, 1, GlobalInfo);
ListDescType.Add(DescSlope);
MergedScreening.ListDescriptors.AddNew(DescSlope);
Idesc++;
}
MergedScreening.ListDescriptors.CurrentSelectedDescriptor = 0;
foreach (cPlate CurrentPlate in CompleteScreening.ListPlatesAvailable)
{
cPlate NewPlate = new cPlate("Cpds", CurrentPlate.Name + " Merged", MergedScreening);
// check if the plate exist already
MergedScreening.AddPlate(NewPlate);
foreach (cDRC_Region CurrentRegion in CurrentPlate.ListDRCRegions)
{
List<cDescriptor> LDesc = new List<cDescriptor>();
Idesc = 0;
int IDESCBase = 0;
for (int i = 0; i < CompleteScreening.ListDescriptors.Count; i++)
{
if (!CompleteScreening.ListDescriptors[i].IsActive()) continue;
cDRC CurrentDRC = CurrentRegion.GetDRC(CompleteScreening.ListDescriptors[IDESCBase++]);
cDescriptor Desc_EC50 = new cDescriptor(CurrentDRC.EC50, ListDescType[Idesc++], CompleteScreening);
LDesc.Add(Desc_EC50);
cDescriptor Desc_Top = new cDescriptor(CurrentDRC.Top, ListDescType[Idesc++], CompleteScreening);
LDesc.Add(Desc_Top);
cDescriptor Desc_Bottom = new cDescriptor(CurrentDRC.Bottom, ListDescType[Idesc++], CompleteScreening);
LDesc.Add(Desc_Bottom);
cDescriptor Desc_Slope = new cDescriptor(CurrentDRC.Slope, ListDescType[Idesc++], CompleteScreening);
LDesc.Add(Desc_Slope);
}
cWell NewWell = new cWell(LDesc, CurrentRegion.PosXMin + 1, CurrentRegion.PosYMin + 1, MergedScreening, NewPlate);
NewWell.Name = "DRC [" + CurrentRegion.PosXMin + ":" + CurrentRegion.PosYMin + "]";
NewPlate.AddWell(NewWell);
}
}
// PanelList[0].CurrentScreening.ListPlatesActive.Clear();
// PanelList[0].CurrentScreening.GlobalInfo.WindowHCSAnalyzer.RefreshInfoScreeningRichBox();
MergedScreening.ListPlatesActive = new cExtendPlateList();
for (int i = 0; i < MergedScreening.ListPlatesAvailable.Count; i++)
{
MergedScreening.ListPlatesActive.Add(MergedScreening.ListPlatesAvailable[i]);
// MergedScreening.GlobalInfo.WindowHCSAnalyzer.toolStripcomboBoxPlateList.Items.Add(PanelList[0].CurrentScreening.ListPlatesActive[i].Name);
}
//PanelList[0].CurrentScreening.CurrentDisplayPlateIdx = 0;
//PanelList[0].CurrentScreening.GlobalInfo.WindowHCSAnalyzer.toolStripcomboBoxPlateList.SelectedIndex = 0;
//PanelList[0].CurrentScreening.GetCurrentDisplayPlate().DisplayDistribution(PanelList[0].CurrentScreening.ListDescriptors.CurrentSelectedDescriptor, false);
CompleteScreening.ListDescriptors = MergedScreening.ListDescriptors;
CompleteScreening.ListPlatesAvailable = MergedScreening.ListPlatesAvailable;
CompleteScreening.ListPlatesActive = MergedScreening.ListPlatesActive;
CompleteScreening.UpDatePlateListWithFullAvailablePlate();
for (int idxP = 0; idxP < CompleteScreening.ListPlatesActive.Count; idxP++)
CompleteScreening.ListPlatesActive[idxP].UpDataMinMax();
CompleteScreening.GetCurrentDisplayPlate().DisplayDistribution(CompleteScreening.ListDescriptors.CurrentSelectedDescriptor, true);
}
