private FillSource _GetCurrentFillSource()
{
AnalyzeArea analyzeArea = this._GetCurrentAnalyzeArea();
Bitmap analyzeAreaImage = analyzeArea.GetImage(this._Project.X, this._Project.Y);
int[,] labels = new int[analyzeAreaImage.Width, analyzeAreaImage.Height];
for (int x = 0; x < analyzeAreaImage.Width; x++)
{
for (int y = 0; y < analyzeAreaImage.Height; y++)
{
Color color = analyzeAreaImage.GetPixel(x, y);
if (color.R == 255 && color.G == 255 && color.B == 255)
{
labels[x, y] = 1;
}
else
{
labels[x, y] = 0;
}
}
}
ConnectedComponents components = new ConnectedComponents(labels, 1);
return(new FillSource(components, 1));
}
private void dataAnalyzeAreas_SelectionChanged(object sender, EventArgs e)
{
DataGridViewCell cell = dataAnalyzeAreas.SelectedCells[0];
nudT.Value = cell.ColumnIndex + 1;
this.checkEnabled.CheckedChanged -= new System.EventHandler(this.checkEnabled_CheckedChanged);
this.nudX.ValueChanged -= new System.EventHandler(this.nudX_ValueChanged);
this.nudY.ValueChanged -= new System.EventHandler(this.nudY_ValueChanged);
this.nudR.ValueChanged -= new System.EventHandler(this.nudR_ValueChanged);
this.nudThreshold.ValueChanged -= new System.EventHandler(this.nudThreshold_ValueChanged);
AnalyzeArea analyzeArea = this._GetCurrentAnalyzeArea();
checkEnabled.Checked = analyzeArea.Enabled;
nudX.Value = analyzeArea.X == -1 ? 0 : analyzeArea.X;
nudY.Value = analyzeArea.Y == -1 ? 0 : analyzeArea.Y;
nudR.Value = analyzeArea.R == 0 ? this._Project.X * Project.DefaultR / 100 : analyzeArea.R;
//nudR.Value = analyzeArea.R == 0 ? Project.DefaultR : analyzeArea.R;
nudThreshold.Value = analyzeArea.Threshold == 0 ? Project.DefaultThreshold : analyzeArea.Threshold;
this.checkEnabled.CheckedChanged += new System.EventHandler(this.checkEnabled_CheckedChanged);
this.nudX.ValueChanged += new System.EventHandler(this.nudX_ValueChanged);
this.nudY.ValueChanged += new System.EventHandler(this.nudY_ValueChanged);
this.nudR.ValueChanged += new System.EventHandler(this.nudR_ValueChanged);
this.nudThreshold.ValueChanged += new System.EventHandler(this.nudThreshold_ValueChanged);
}
public void SetFromAnalyzeArea(AnalyzeArea analyzeArea)
{
this.SetAttributes(
analyzeArea.Enabled,
analyzeArea.X,
analyzeArea.Y,
analyzeArea.R,
analyzeArea.Threshold
);
}
private void _SetTargetValueZ()
{
if (dataAnalyzeAreaSets.SelectedRows.Count > 0)
{
DataGridViewRow s_row = dataAnalyzeAreaSets.CurrentRow;
AnalyzeAreaSet s_analyzeAreaSet = (AnalyzeAreaSet)s_row.DataBoundItem;
//int selectZ = this._Analyzer.GetZ(s_analyzeAreaSet, scrollT.Value);
int selectZ = 1;
for (int z = 1; z <= this._Project.Z; z++)
{
AnalyzeArea aa = s_analyzeAreaSet.GetAnalyzeArea(z, scrollT.Value);
if (aa.Enabled)
{
selectZ = z;
break;
}
}
if (scrollZ.Maximum < selectZ)
{
scrollZ.Maximum = selectZ;
}
if (scrollZ.Minimum > selectZ)
{
scrollZ.Minimum = selectZ;
}
if (nudZ.Maximum < selectZ)
{
nudZ.Maximum = selectZ;
}
if (nudZ.Minimum > selectZ)
{
nudZ.Minimum = selectZ;
}
scrollZ.Value = selectZ;
nudZ.Value = selectZ;
//AnalyzeArea analyzeArea = this._GetCurrentAnalyzeArea();
//nudThreshold.Value = analyzeArea.Threshold;
scrollZ.Enabled = false;
nudZ.Enabled = false;
}
else
{
scrollZ.Enabled = true;
nudZ.Enabled = true;
}
}
public int AnalyzeArea(AnalyzeArea analyzeArea)
{
if (analyzeArea.Enabled == false)
{
return(0);
}
Bitmap image = new Bitmap(this.GetImage());
Bitmap analyzeAreaImage = analyzeArea.GetImage(image.Width, image.Height);
int pixels = 0;
int startX = analyzeArea.X - analyzeArea.R - 1;
startX = startX < 0 ? 0 : startX;
int endX = analyzeArea.X + analyzeArea.R + 1;
endX = endX > image.Width ? image.Width : endX;
int startY = analyzeArea.Y - analyzeArea.R - 1;
startY = startY < 0 ? 0 : startY;
int endY = analyzeArea.Y + analyzeArea.R + 1;
endY = endY > image.Height ? image.Height : endY;
for (int x = startX; x < endX; x++)
{
for (int y = startY; y < endY; y++)
{
Color analyzeAreaColor = analyzeAreaImage.GetPixel(x, y);
if (analyzeAreaColor.R == 255 && analyzeAreaColor.G == 255 && analyzeAreaColor.B == 255)
{
Color color = image.GetPixel(x, y);
int average = (int)((color.R + color.G + color.B) / 3);
if (average > analyzeArea.Threshold)
{
pixels++;
}
}
}
}
return(pixels);
}
private void _SetCurrentAnalyzeArea()
{
AnalyzeArea analyzeArea = this._GetCurrentAnalyzeArea();
this.AnalyzeAreaSet.Name = textName.Text;
analyzeArea.Enabled = checkEnabled.Checked;
analyzeArea.X = (int)nudX.Value;
analyzeArea.Y = (int)nudY.Value;
analyzeArea.R = (int)nudR.Value;
analyzeArea.Threshold = (int)nudThreshold.Value;
DataGridViewCell cell = dataAnalyzeAreas[scrollT.Value - 1, 0];
cell.Value = analyzeArea.ToString();
}
private void _ClearCurrentAnalyzeArea(int t = 0)
{
if (t == 0)
{
t = scrollT.Value;
}
for (int z = 1; z <= this._Project.Z; z++)
{
AnalyzeArea analyzeArea = this.AnalyzeAreaSet.GetAnalyzeArea(z, t);
this.AnalyzeAreaSet.Name = textName.Text;
analyzeArea.Enabled = false;
analyzeArea.X = -1;
analyzeArea.Y = -1;
analyzeArea.R = 0;
analyzeArea.Threshold = 0;
}
}
public int GetZ(AnalyzeAreaSet analyzeAreaSet, int t)
{
int targetZ = 1;
//AnalyzeArea analyzeArea = analyzeAreaSet.GetAnalyzeArea(1, t);
AnalyzeArea analyzeArea = new AnalyzeArea();
for (int z = 1; z <= this._Project.Z; z++)
{
analyzeArea = analyzeAreaSet.GetAnalyzeArea(z, t);
if (analyzeArea.Enabled)
{
targetZ = z;
break;
}
}
return(targetZ);
}
public bool IsValid()
{
if (this.Name == "")
{
return(false);
}
for (int z = 0; z < this.AnalyzeAreas.GetLength(0); z++)
{
for (int t = 0; t < this.AnalyzeAreas.GetLength(1); t++)
{
AnalyzeArea analyzeArea = this.AnalyzeAreas[z, t];
if (analyzeArea.IsValid() == false)
{
return(false);
}
}
}
return(true);
}
public double[] AnalyzeSpot(AnalyzeAreaSet analyzeAreaSet, int t, int threshold)
{
int targetZ = 0;
//AnalyzeArea analyzeArea = analyzeAreaSet.GetAnalyzeArea(1, t);
AnalyzeArea analyzeArea = new AnalyzeArea();
for (int z = 1; z <= this._Project.Z; z++)
{
analyzeArea = analyzeAreaSet.GetAnalyzeArea(z, t);
if (analyzeArea.Enabled)
{
targetZ = z;
break;
}
}
if (targetZ == 0)
{
return(new double[3]);
}
double[] spots = new double[3];
MicroImage microImage473 = this.GetMicroImage(targetZ, t, "473");
MicroImage microImage561 = this.GetMicroImage(targetZ, t, "561");
//spots[0] = analyzeArea.Count473;
//spots[1] = analyzeArea.Count561;
spots[0] = microImage473.AnalyzeSpot(analyzeArea, threshold);
spots[1] = microImage561.AnalyzeSpot(analyzeArea, threshold);
//Integrated Density
spots[2] = microImage473.AnalyzeLuminance(analyzeArea, threshold);
return(spots);
}
public double AnalyzeLuminance(AnalyzeArea analyzeArea, int threshold)
{
if (analyzeArea.Enabled == false)
{
return(0);
}
Bitmap image = this.GetBinarizedImage(threshold);
//Bitmap analyzeAreaImage = analyzeArea.GetImage(image.Width, image.Height);
Tiff tif = this.GetImage16Bit();
int stride = tif.ScanlineSize();
byte[] buffer = new byte[stride];
//for (int i = 0; i < image.Height; i++)
//{
// tif.ReadScanline(buffer, i);
// for (int src = 0, dst = 0; src < buffer.Length; dst++)
// {
// int value16 = buffer[src++];
// value16 = value16 + (buffer[src++] << 8);
// }
//}
double luminance = 0;
int startX = analyzeArea.X - analyzeArea.R - 1;
startX = startX < 0 ? 0 : startX;
int endX = analyzeArea.X + analyzeArea.R + 1;
endX = endX > image.Width ? image.Width : endX;
int startY = analyzeArea.Y - analyzeArea.R - 1;
startY = startY < 0 ? 0 : startY;
int endY = analyzeArea.Y + analyzeArea.R + 1;
endY = endY > image.Height ? image.Height : endY;
//選択部分の取得
Bitmap imageEllipse = new Bitmap(image.Width, image.Height);
Graphics g = Graphics.FromImage(imageEllipse);
g.Clear(Color.Black);
g.FillEllipse(Brushes.White, analyzeArea.X - analyzeArea.R, analyzeArea.Y - analyzeArea.R, analyzeArea.R * 2, analyzeArea.R * 2);
g.Dispose();
for (int y = startY; y < endY; y++)
{
tif.ReadScanline(buffer, y);
for (int x = startX; x < endX; x++)
{
Color analyzeAreaColor = image.GetPixel(x, y);
Color ellipseColor = imageEllipse.GetPixel(x, y);
if (analyzeAreaColor.R == 255 && analyzeAreaColor.G == 255 && analyzeAreaColor.B == 255 && ellipseColor.R == 255 && ellipseColor.G == 255 && ellipseColor.B == 255)
{
int src = x * 2;
int value16 = buffer[src++];
value16 = value16 + (buffer[src++] << 8);
luminance += value16;
}
}
}
return(luminance);
}
public double AnalyzeSpot(AnalyzeArea analyzeArea, int threshold)
{
double return_data = 0;
if (analyzeArea.Enabled == false)
{
return(return_data);
}
Bitmap image = this.GetBinarizedImage(threshold);
Bitmap analyzeAreaImage = analyzeArea.GetImage(image.Width, image.Height);
int startX = analyzeArea.X - analyzeArea.R - 1;
startX = startX < 0 ? 0 : startX;
int endX = analyzeArea.X + analyzeArea.R + 1;
endX = endX > image.Width ? image.Width : endX;
int startY = analyzeArea.Y - analyzeArea.R - 1;
startY = startY < 0 ? 0 : startY;
int endY = analyzeArea.Y + analyzeArea.R + 1;
endY = endY > image.Height ? image.Height : endY;
ConnectedComponents components = ConnectedComponents.Analyze(image);
Dictionary <int, int> labels = new Dictionary <int, int>();
//選択部分の取得
Bitmap imageEllipse = new Bitmap(image.Width, image.Height);
Graphics g = Graphics.FromImage(imageEllipse);
g.Clear(Color.Black);
g.FillEllipse(Brushes.White, analyzeArea.X - analyzeArea.R, analyzeArea.Y - analyzeArea.R, analyzeArea.R * 2, analyzeArea.R * 2);
g.Dispose();
for (int x = startX; x < endX; x++)
{
for (int y = startY; y < endY; y++)
{
int label = components.Labels[x, y];
Color color = imageEllipse.GetPixel(x, y);
if (label <= 0 || (color.R == 0 && color.G == 0 && color.B == 0))
{
continue;
}
if (labels.ContainsKey(label))
{
labels[label]++;
}
else
{
labels[label] = 1;
}
}
}
return(labels.Count);
}
private void _Render()
{
Cursor oldCursor = Cursor.Current;
Cursor.Current = Cursors.WaitCursor;
if (this._Analyzer != null)
{
menuProjectSave.Enabled = true;
menuProjectSaveAs.Enabled = true;
menuTool.Enabled = true;
panel.Enabled = true;
checkDrawArea.Enabled = dataAnalyzeAreaSets.SelectedRows.Count > 0 ? true : false;
checkBinarize.Enabled = dataAnalyzeAreaSets.SelectedRows.Count > 0 ? true : false;
buttonAnalyzer.Enabled = dataAnalyzeAreaSets.SelectedRows.Count > 0 ? true : false;
nudThreshold.Enabled = dataAnalyzeAreaSets.SelectedRows.Count > 0 ? true : false;
this._SetTargetValueZ();
foreach (DataGridViewRow row in dataAnalyzeAreaSets.Rows)
{
if (this._Project.T <= row.Cells.Count)
{
AnalyzeAreaSet analyzeAreaSet = (AnalyzeAreaSet)row.DataBoundItem;
row.HeaderCell.Value = analyzeAreaSet.Name;
for (int t = 1; t <= this._Project.T; t++)
{
int targetZ = 1;
for (int z = 1; z <= this._Project.Z; z++)
{
AnalyzeArea aa = analyzeAreaSet.GetAnalyzeArea(z, t);
if (aa.Enabled)
{
targetZ = z;
break;
}
}
row.Cells[t - 1].Value = analyzeAreaSet.GetAnalyzeArea(targetZ, t).ToString();
}
}
}
dataAnalyzeAreaSets.AutoResizeRowHeadersWidth(DataGridViewRowHeadersWidthSizeMode.AutoSizeToAllHeaders);
MicroImage microImage;
if (radioButtonPic473.Checked)
{
microImage = this._GetCurrentMicroImage("473");
}
else
{
microImage = this._GetCurrentMicroImage("561");
}
Image image;
if (checkBinarize.Checked == true)
{
int threshold = (int)nudThreshold.Value;
image = microImage.GetBinarizedImage(threshold);
}
else
{
image = microImage.GetImage();
}
if (checkDrawArea.Checked == true && dataAnalyzeAreaSets.SelectedRows.Count > 0)
{
AnalyzeArea analyzeArea = this._GetCurrentAnalyzeArea();
if (analyzeArea.Enabled == true)
{
Image tmp = new Bitmap(image.Width, image.Height);
Graphics g = Graphics.FromImage(tmp);
g.DrawImage(image, 0, 0, image.Width, image.Height);
Pen pen = new Pen(Color.FromArgb(191, Color.LightGray));
g.DrawEllipse(
pen,
analyzeArea.X - analyzeArea.R,
analyzeArea.Y - analyzeArea.R,
analyzeArea.R * 2,
analyzeArea.R * 2
);
g.Dispose();
image = tmp;
}
}
pictureMicroImage.Image = image;
}
else
{
menuProjectSave.Enabled = false;
menuProjectSaveAs.Enabled = false;
menuTool.Enabled = false;
chartSpot.Visible = false;
buttonExportAsCSV.Enabled = false;
buttonAnalyzer.Enabled = false;
nudThreshold.Enabled = false;
panel.Enabled = false;
}
Cursor.Current = oldCursor;
labelStatus.Text = this._Flash;
this._Flash = "";
}