public void ParseDataset(string datasetDirectory, Dictionary<string, BreastCancerSampleItem> sampleMap)
{
var files = GeoUtils.GetGsmNameFileMap(datasetDirectory);
var dirname = Path.GetFileName(datasetDirectory);
//The status of ER, PR is on the sample title
var samples = new GseSeriesMatrixReader().ReadFromDirectory(datasetDirectory);
foreach (var a in samples)
{
var filename = a.Key.ToLower();
if (files.ContainsKey(filename.ToLower()))
{
var title = a.Value[GsmConsts.SampleTitle];
var m = r.Match(title.First());
var er = m.Groups[1].Value.Equals("p") ? "pos" : "neg";
var pr = m.Groups[2].Value.Equals("p") ? "pos" : "neg";
var ts = m.Groups[3].Value;
var n = m.Groups[4].Value;
var grade = m.Groups[5].Value;
var key = filename.ToUpper();
if (!sampleMap.ContainsKey(key))
{
sampleMap[key] = new BreastCancerSampleItem(dirname, filename.ToUpper());
}
BreastCancerSampleItem item = sampleMap[key];
item.ER = er;
item.PR = pr;
item.TumorStatus = ts;
item.Grade = grade;
}
}
}
public override IEnumerable <string> Process()
{
var gses = File.ReadAllLines(options.GseListFile).Where(m => !string.IsNullOrWhiteSpace(m)).ToList().ConvertAll(m => m.Split(new[] { '\t', ' ' })[0]).ToArray();
var gseInfoMap = new Dictionary <string, Dictionary <string, Dictionary <string, List <string> > > >();
for (int gseIndex = 0; gseIndex < gses.Length; gseIndex++)
{
var gse = gses[gseIndex];
Func <GsmRecord, bool> accept;
if (!options.AcceptMap.TryGetValue(gse, out accept))
{
accept = m => true;
}
Func <Dictionary <string, List <string> >, bool> acceptDescription;
if (!options.AcceptDescriptionMap.TryGetValue(gse, out acceptDescription))
{
acceptDescription = null;
}
var sql = string.Format(@"select gse.gse, gsm.supplementary_file, gsm.title, gsm.gpl, gsm.source_name_ch1
from gse
JOIN gse_gsm ON gse.gse=gse_gsm.gse
JOIN gsm ON gse_gsm.gsm=gsm.gsm
where
gse.gse == '{0}'
and (gsm.supplementary_file like '%CEL.gz%' or gsm.supplementary_file like '%cel.gz%')
group by gse.gse, gsm.supplementary_file
", gse);
SQLiteDBHelper sqlite = new SQLiteDBHelper(options.GeoMetaDatabase);
Progress.SetMessage("{0}/{1}: querying files ...", gseIndex + 1, gses.Length);
var data = sqlite.ExecuteDataTable(sql, null);
var records = new List <GsmRecord>();
for (int row = 0; row < data.Rows.Count; row++)
{
var rec = new GsmRecord()
{
GSE = data.Rows[row].ItemArray[0].ToString(),
Url = data.Rows[row].ItemArray[1].ToString(),
Title = data.Rows[row].ItemArray[2].ToString(),
GPL = data.Rows[row].ItemArray[3].ToString(),
SourceName = data.Rows[row].ItemArray[4].ToString()
};
records.Add(rec);
}
var totalValids = records.Where(m => accept(m)).Count();
int currentValid = 0;
foreach (var rec in records)
{
var file = rec.Url.Split(';').Where(m => m.Trim().ToLower().EndsWith("cel.gz")).First();
var dataDir = builder.DataDir + "/";
var dir = dataDir + rec.GSE;
var gzipped = dir + "/" + Path.GetFileName(file);
var tmp = gzipped + "tmp";
if (!accept(rec))
{
DeleteGzippedFile(gzipped);
continue;
}
currentValid++;
var prefix = string.Format("{0}/{1} ~ {2} : {3}/{4}", gseIndex + 1, gses.Length, rec.GSE, currentValid, totalValids);
Progress.SetMessage(prefix + " ~ " + file);
if (!Directory.Exists(dataDir))
{
Directory.CreateDirectory(dataDir);
}
if (!Directory.Exists(dir))
{
Directory.CreateDirectory(dir);
}
if (acceptDescription != null)
{
Dictionary <string, Dictionary <string, List <string> > > curInfoMap;
if (!gseInfoMap.TryGetValue(rec.GSE, out curInfoMap))
{
if (!GseSeriesMatrixReader.HasMatrixFiles(dir))
{
new GseMatrixDownloader(new GseMatrixDownloaderOptions()
{
InputDirectory = dir
}).Process();
}
if (!GseSeriesMatrixReader.HasMatrixFiles(dir))
{
throw new Exception("Failed to download matrix file for " + gse);
}
curInfoMap = new GseSeriesMatrixReader().ReadDescriptionFromDirectory(dir);
gseInfoMap[rec.GSE] = curInfoMap;
}
var gsmName = Path.GetFileName(file).StringBefore(".cel.gz").StringBefore(".CEL.gz");
var sampleInfoMap = curInfoMap[gsmName];
if (!acceptDescription(sampleInfoMap))
{
DeleteGzippedFile(gzipped);
continue;
}
}
if (File.Exists(gzipped) && new FileInfo(gzipped).Length == 0)
{
File.Delete(gzipped);
}
if (!File.Exists(gzipped))
{
Progress.SetMessage(prefix + " ~ downloading " + file + " ...");
if (!WebUtils.DownloadFile(file, tmp))
{
Console.Error.WriteLine("Download {0} failed.", file);
break;
}
File.Move(tmp, gzipped);
}
}
}
var nocels = (from gse in gses
let dir = builder.DataDir + "/" + gse
where !Directory.Exists(dir)
select gse).Merge("\n");
if (!string.IsNullOrEmpty(nocels))
{
throw new Exception("No cel file found for\n" + nocels);
}
return(new[] { options.RootDirectory });
}
public override IEnumerable<string> Process()
{
var gses = File.ReadAllLines(options.GseListFile).Where(m => !string.IsNullOrWhiteSpace(m)).ToList().ConvertAll(m => m.Split(new[] { '\t', ' ' })[0]).ToArray();
var gseInfoMap = new Dictionary<string, Dictionary<string, Dictionary<string, List<string>>>>();
for (int gseIndex = 0; gseIndex < gses.Length; gseIndex++)
{
var gse = gses[gseIndex];
Func<GsmRecord, bool> accept;
if (!options.AcceptMap.TryGetValue(gse, out accept))
{
accept = m => true;
}
Func<Dictionary<string, List<string>>, bool> acceptDescription;
if (!options.AcceptDescriptionMap.TryGetValue(gse, out acceptDescription))
{
acceptDescription = null;
}
var sql = string.Format(@"select gse.gse, gsm.supplementary_file, gsm.title, gsm.gpl, gsm.source_name_ch1
from gse
JOIN gse_gsm ON gse.gse=gse_gsm.gse
JOIN gsm ON gse_gsm.gsm=gsm.gsm
where
gse.gse == '{0}'
and (gsm.supplementary_file like '%CEL.gz%' or gsm.supplementary_file like '%cel.gz%')
group by gse.gse, gsm.supplementary_file
", gse);
SQLiteDBHelper sqlite = new SQLiteDBHelper(options.GeoMetaDatabase);
Progress.SetMessage("{0}/{1}: querying files ...", gseIndex + 1, gses.Length);
var data = sqlite.ExecuteDataTable(sql, null);
var records = new List<GsmRecord>();
for (int row = 0; row < data.Rows.Count; row++)
{
var rec = new GsmRecord()
{
GSE = data.Rows[row].ItemArray[0].ToString(),
Url = data.Rows[row].ItemArray[1].ToString(),
Title = data.Rows[row].ItemArray[2].ToString(),
GPL = data.Rows[row].ItemArray[3].ToString(),
SourceName = data.Rows[row].ItemArray[4].ToString()
};
records.Add(rec);
}
var totalValids = records.Where(m => accept(m)).Count();
int currentValid = 0;
foreach (var rec in records)
{
var file = rec.Url.Split(';').Where(m => m.Trim().ToLower().EndsWith("cel.gz")).First();
var dataDir = builder.DataDir + "/";
var dir = dataDir + rec.GSE;
var gzipped = dir + "/" + Path.GetFileName(file);
var tmp = gzipped + "tmp";
if (!accept(rec))
{
DeleteGzippedFile(gzipped);
continue;
}
currentValid++;
var prefix = string.Format("{0}/{1} ~ {2} : {3}/{4}", gseIndex + 1, gses.Length, rec.GSE, currentValid, totalValids);
Progress.SetMessage(prefix + " ~ " + file);
if (!Directory.Exists(dataDir))
{
Directory.CreateDirectory(dataDir);
}
if (!Directory.Exists(dir))
{
Directory.CreateDirectory(dir);
}
if (acceptDescription != null)
{
Dictionary<string, Dictionary<string, List<string>>> curInfoMap;
if (!gseInfoMap.TryGetValue(rec.GSE, out curInfoMap))
{
if (!GseSeriesMatrixReader.HasMatrixFiles(dir))
{
new GseMatrixDownloader(new GseMatrixDownloaderOptions()
{
InputDirectory = dir
}).Process();
}
if (!GseSeriesMatrixReader.HasMatrixFiles(dir))
{
throw new Exception("Failed to download matrix file for " + gse);
}
curInfoMap = new GseSeriesMatrixReader().ReadDescriptionFromDirectory(dir);
gseInfoMap[rec.GSE] = curInfoMap;
}
var gsmName = Path.GetFileName(file).StringBefore(".cel.gz").StringBefore(".CEL.gz");
var sampleInfoMap = curInfoMap[gsmName];
if (!acceptDescription(sampleInfoMap))
{
DeleteGzippedFile(gzipped);
continue;
}
}
if (File.Exists(gzipped) && new FileInfo(gzipped).Length == 0)
{
File.Delete(gzipped);
}
if (!File.Exists(gzipped))
{
Progress.SetMessage(prefix + " ~ downloading " + file + " ...");
if (!WebUtils.DownloadFile(file, tmp))
{
Console.Error.WriteLine("Download {0} failed.", file);
break;
}
File.Move(tmp, gzipped);
}
}
}
var nocels = (from gse in gses
let dir = builder.DataDir + "/" + gse
where !Directory.Exists(dir)
select gse).Merge("\n");
if (!string.IsNullOrEmpty(nocels))
{
throw new Exception("No cel file found for\n" + nocels);
}
return new[] { options.RootDirectory };
}
