private void AnalyseSequence(cImage SeqToAnalyse)
{
// //FormForThumbnails WindThumbnail = new FormForThumbnails();
// //for (int k = 0; k < ListFormForControl.Count; k++)
// //{
// // FormForControl Obj = ListFormForControl.ElementAt(ListFormForControl.Count - k-1);
// // Obj.DisplayThumbnail(WindThumbnail);
// //}
// //WindThumbnail.Show();
// //return;
#region initialization
if (SeqToAnalyse == null) return;
// create World
if (Current3DWorld == null)
{
Current3DWorld = new c3DNewWorld(new cPoint3D(SeqToAnalyse.Width, SeqToAnalyse.Height, SeqToAnalyse.Depth),
new cPoint3D(SeqToAnalyse.Resolution));
}
else
{
// // Current3DWorld.ren1.
// Current3DWorld.Terminate();
Current3DWorld = null;
Current3DWorld = new c3DNewWorld(new cPoint3D(SeqToAnalyse.Width, SeqToAnalyse.Height, SeqToAnalyse.Depth),
new cPoint3D(SeqToAnalyse.Resolution));
}
for (int k = 0; k < ListFormForControl.Count; k++)
{
FormForControl Obj = ListFormForControl.ElementAt(ListFormForControl.Count - k-1);
if (Obj.numericUpDownChannel.Value >= SeqToAnalyse.GetNumChannels())
{
MessageBox.Show("Wrong number of channels", "Error", MessageBoxButtons.OK, MessageBoxIcon.Error);
return;
}
}
// // Current3DWorld.ListObjControl = ListFormForControl;
// // dataGridViewForClassif.DataSource = CurrentTable;
// if (DataGrid == null)
// {
// DataGrid = new FormForDataGridView();
// DataGrid.Show();
// }
// Current3DWorld.SetLinkToDataGridView(DataGrid.dataGridViewForResults);
Current3DWorld.ListMetaObjectList = new List<cMetaBiologicalObjectList>();
cMetaBiologicalObjectList ListMetacells = new cMetaBiologicalObjectList("List Meta Objects");
Current3DWorld.ListMetaObjectList.Add(ListMetacells);
#endregion
#region Detection
for (int k = 0; k < ListFormForControl.Count; k++)
{
FormForControl Obj = ListFormForControl.ElementAt(ListFormForControl.Count - k -1);
if (Obj.radioButtonIsVolume.Checked) // we have to detect a volume !
{
cVolumeDetection Detection = new cVolumeDetection(SeqToAnalyse, (int)Obj.numericUpDownChannel.Value, Obj.WindowForVolumeDetection.checkBoxIsBorderKill.Checked);
Detection.SetShift(new cPoint3D((float)Obj.WindowForPreProcessing.numericUpDownShiftX.Value, (float)Obj.WindowForPreProcessing.numericUpDownShiftY.Value, (float)Obj.WindowForPreProcessing.numericUpDownShiftZ.Value));
if ((Obj.checkBoxPreProcessings.Checked == false) || (Obj.WindowForPreProcessing.checkBoxMedianIsDisabled.Checked))
{
Detection.SetMedian(-1);
Detection.SetShift(new cPoint3D(0, 0, 0));
}
else
{
Detection.SetMedian((int)Obj.WindowForPreProcessing.numericUpDownMedianKernel.Value);
Detection.SetShift(new cPoint3D((float)Obj.WindowForPreProcessing.numericUpDownShiftX.Value, (float)Obj.WindowForPreProcessing.numericUpDownShiftY.Value, (float)Obj.WindowForPreProcessing.numericUpDownShiftZ.Value));
}
if (Obj.WindowForVolumeDetection.checkBoxVolumeSmooth.Checked)
Detection.MeshSmoother = new cMeshSmoother((int)Obj.WindowForVolumeDetection.numericUpDownSmoothIterations.Value);
if ((Obj.comboBoxContainer.SelectedItem !=null) && (Obj.comboBoxContainer.SelectedItem.ToString() != ""))
{
Detection.SetContainers(Current3DWorld.GetListBiologicalObjectsOfType(Obj.comboBoxContainer.Items[0].ToString()));
if (Obj.InOrOut.radioButtonIn.Checked) Detection.SetContainersMode(0);
else Detection.SetContainersMode(1);
}
else
Detection.SetContainers(null);
if (Obj.WindowForVolumeDetection.checkBoxIsRegionGrowing.Checked == false) // no region growing direct detection
{
Obj.AssociatedBiologicalObjectList = Detection.IntensityThreshold((float)Obj.WindowForVolumeDetection.numericUpDownIntensityThreshold.Value,
(float)Obj.WindowForVolumeDetection.numericUpDownVolumeMinVol.Value,
(float)Obj.WindowForVolumeDetection.numericUpDownVolumeMaxVol.Value);
}
else // region growing
{
if (Obj.WindowForVolumeDetection.radioButtonRegionsBased.Checked) // start from a segmentation
{
if (Obj.WindowForVolumeDetection.radioButtonVolumeDetectionNew.Checked) // a new segmentation has to be performed
{
// // Image3D CurrentPatch = new Image3D(2 * Radius, 2 * Radius, 1, CurrentImageToProcess.NumBands);
cImage CurrentBinary = new cImage(SeqToAnalyse.Width, SeqToAnalyse.Height, SeqToAnalyse.Depth, 1);
for (int i = 0; i < CurrentBinary.ImageSize; i++)
{
if (SeqToAnalyse.SingleChannelImage[(int)Obj.numericUpDownChannel.Value].Data[i] >= (float)Obj.WindowForVolumeDetection.numericUpDownIntensityThreshold.Value)
CurrentBinary.SingleChannelImage[0].Data[i] = 1;
}
eConnectivity CurrentConnectivity = eConnectivity.TWOD_4;
if (CurrentBinary.Depth > 1)
CurrentConnectivity = eConnectivity.THREED_6;
ConnectedComponentSet OFNucleus = new ConnectedComponentSet(CurrentBinary, null, null, 0, CurrentConnectivity, (int)Obj.WindowForVolumeDetection.numericUpDownRegionBasedMinArea.Value, (int)Obj.WindowForVolumeDetection.numericUpDownRegionBasedMaxArea.Value);
// // RegionGrowing = new cRegionGrowing(OFNucleus, NewSeq[0], (int)numericUpDownChannelForRegionGrowing.Value, (float)numericUpDownIntensityForRegionGrowing.Value, (int)numericUpDownMaxNucleusArea.Value);
int NumIterations = -1;
if (Obj.WindowForVolumeDetection.checkBoxIsConvergence.Checked == false) NumIterations = (int)Obj.WindowForVolumeDetection.numericUpDownIterationNumber.Value;
float MergingRatio = (float)((Obj.WindowForVolumeDetection.trackBarMergingStrength.Maximum - Obj.WindowForVolumeDetection.trackBarMergingStrength.Value) / 100.0);
Obj.AssociatedBiologicalObjectList = Detection.RegionGrowing(OFNucleus,
(float)Obj.WindowForVolumeDetection.numericUpDownIntensityForRegionGrowing.Value,
(float)Obj.WindowForVolumeDetection.numericUpDownVolumeMinVol.Value,
(float)Obj.WindowForVolumeDetection.numericUpDownVolumeMaxVol.Value, NumIterations, MergingRatio);
}
// else
// {
// throw new System.ArgumentException("Not implemented", "Error");
// }
// }
// else // start from seeds points
// {
// // first gather the seeds
// if (Obj.WindowForVolumeDetection.comboBoxExistingSpotsDetected.Items.Count == 0) break;
// List<cBiological3DObject> ListSeeds = Current3DWorld.GetListBiologicalObjectsOfType(Obj.WindowForVolumeDetection.comboBoxExistingSpotsDetected.Items[0].ToString());
// int NumIterations = -1;
// if (Obj.WindowForVolumeDetection.checkBoxIsConvergence.Checked == false) NumIterations = (int)Obj.WindowForVolumeDetection.numericUpDownIterationNumber.Value;
// float MergingRatio = (float)((Obj.WindowForVolumeDetection.trackBarMergingStrength.Maximum - Obj.WindowForVolumeDetection.trackBarMergingStrength.Value) / 100.0);
// Obj.AssociatedBiologicalObjectList = Detection.RegionGrowing(ListSeeds,
// (float)Obj.WindowForVolumeDetection.numericUpDownIntensityForRegionGrowing.Value,
// (float)Obj.WindowForVolumeDetection.numericUpDownVolumeMinVol.Value,
// (float)Obj.WindowForVolumeDetection.numericUpDownVolumeMaxVol.Value, NumIterations, MergingRatio);
}
}
if (Obj.AssociatedBiologicalObjectList != null)
{
Obj.richTextBoxInfo.AppendText(Obj.AssociatedBiologicalObjectList.Count + " objects detected.");
for (int i = 0; i < Obj.AssociatedBiologicalObjectList.Count; i++)
{
cBiological3DVolume TmpVol = (cBiological3DVolume)Obj.AssociatedBiologicalObjectList[i];
Obj.AssociatedBiologicalObjectList[i].SetType(Obj.textBoxName.Text);
// TmpVol.ThumbnailnewImage = imageListForThumbnail.Images[Obj.AssociatedBiologicalObjectList[i].GetType() + ".jpg"];
Obj.AssociatedBiologicalObjectList[i].Name = Obj.textBoxName.Text + " " + i;
// Obj.AssociatedBiologicalObjectList[i].IsMaster = Obj.IsMasterObject();
// List<double> Res = TmpVol.Information.GetInformation();
if (Obj.checkBoxIsDisplayName.Checked)
{
cGeometric3DObject AssociatedText = TmpVol.AttachText(TmpVol.Name, 10, Color.White);
AssociatedText.IsStayInFrontOfCamera = true;
Current3DWorld.AddGeometric3DObject(AssociatedText);
// TmpVol.AddText(TmpVol.Name, Current3DWorld, (double)Obj.ForText.numericUpDownArrowScale.Value, Obj.ForText.buttonChangeColorPositive.BackColor);
}
if (Obj.checkIsBoxPointingArrow.Checked)
{
cGeometric3DObject AssociatedArrow = TmpVol.AttachPointingArrow((double)Obj.ForArrow.numericUpDownArrowScale.Value,
Obj.ForArrow.buttonChangeColorPositive.BackColor);
Current3DWorld.AddGeometric3DObject(AssociatedArrow);
}
Obj.AssociatedBiologicalObjectList[i].SetOpacity((double)Obj.ColorOpacity.numericUpDownValue.Value);
// if the object it the Master then create a meta-object and put it inside
if (Obj.IsMasterObject())
{
cMetaBiologicalObject Cell = new cMetaBiologicalObject(Obj.WindowForMaster.textBoxName.Text + i, Current3DWorld.ListMetaObjectList[0], Obj.AssociatedBiologicalObjectList[i]);
// // Cell.AddObject(Obj.AssociatedBiologicalObjectList[i]);
Current3DWorld.ListMetaObjectList[0].Add(Cell);
}
}
if (Obj.radioButtonColorSingle.Checked)
Current3DWorld.AddBiological3DObjects(Obj.AssociatedBiologicalObjectList, Obj.buttonChangeColorPositive.BackColor, eColorMode.Regular);
else if (Obj.radioButtonColorRandom.Checked)
Current3DWorld.AddBiological3DObjects(Obj.AssociatedBiologicalObjectList, Obj.buttonChangeColorPositive.BackColor, eColorMode.Random);
else if (Obj.radioButtonColorIndexed.Checked)
Current3DWorld.AddBiological3DObjects(Obj.AssociatedBiologicalObjectList, Obj.buttonChangeColorPositive.BackColor, eColorMode.Indexed);
}
}
else if (Obj.RadioButtonIsSpot.Checked)
{
cSpotDetection Detection = new cSpotDetection(SeqToAnalyse, (int)Obj.numericUpDownChannel.Value);
if ((Obj.checkBoxPreProcessings.Checked == false) || (Obj.WindowForPreProcessing.checkBoxMedianIsDisabled.Checked))
{
Detection.SetMedian(-1);
Detection.SetShift(new cPoint3D(0, 0, 0));
}
else
{
Detection.SetMedian((int)Obj.WindowForPreProcessing.numericUpDownMedianKernel.Value);
Detection.SetShift(new cPoint3D((float)Obj.WindowForPreProcessing.numericUpDownShiftX.Value, (float)Obj.WindowForPreProcessing.numericUpDownShiftY.Value, (float)Obj.WindowForPreProcessing.numericUpDownShiftZ.Value));
}
if ((Obj.comboBoxContainer.Items.Count > 0) && (Obj.comboBoxContainer.SelectedItem != null))
{
Detection.SetContainers(Current3DWorld.GetListBiologicalObjectsOfType(Obj.comboBoxContainer.SelectedItem.ToString()));
if (Obj.InOrOut.radioButtonIn.Checked) Detection.SetContainersMode(0);
else Detection.SetContainersMode(1);
}
Obj.AssociatedBiologicalObjectList = Detection.HessianDetection((float)Obj.WindowForSpotDetection.numericUpDownSpotRadius.Value,
(float)Obj.WindowForSpotDetection.numericUpDownIntensityThreshold.Value,
(int)Obj.WindowForSpotDetection.numericUpDownSpotLocality.Value,
(double)Obj.WindowForSpotDetection.numericUpDownSphereDisplayRadius.Value);
Obj.richTextBoxInfo.AppendText(Obj.AssociatedBiologicalObjectList.Count + " objects detected.");
for (int i = 0; i < Obj.AssociatedBiologicalObjectList.Count; i++)
{
cBiologicalSpot TmpSpot = (cBiologicalSpot)Obj.AssociatedBiologicalObjectList[i];
TmpSpot.Name = Obj.textBoxName.Text + " " + i;
TmpSpot.SetType(Obj.textBoxName.Text);
// TmpSpot.ThumbnailnewImage = imageListForThumbnail.Images[Obj.AssociatedBiologicalObjectList[i].GetType() + ".jpg"];
// if (Obj.checkBoxIsDisplayName.Checked)
// TmpSpot.AddText(TmpSpot.Name, Current3DWorld, (double)Obj.ForText.numericUpDownArrowScale.Value, Obj.ForText.buttonChangeColorPositive.BackColor);
if (Obj.checkIsBoxPointingArrow.Checked)
{
cGeometric3DObject AssociatedArrow = TmpSpot.AttachPointingArrow((double)Obj.ForArrow.numericUpDownArrowScale.Value, Obj.ForArrow.buttonChangeColorPositive.BackColor);
Current3DWorld.AddGeometric3DObject(AssociatedArrow);
}
// TmpSpot.SetOpacity((double)Obj.ColorOpacity.numericUpDownValue.Value);
// // if the object it the Master then create a meta-object and put it inside
if (Obj.IsMasterObject())
{
cMetaBiologicalObject Cell = new cMetaBiologicalObject("Cell " + i, Current3DWorld.ListMetaObjectList[0], Obj.AssociatedBiologicalObjectList[i]);
// // Cell.AddObject(Obj.AssociatedBiologicalObjectList[i]);
Current3DWorld.ListMetaObjectList[0].Add(Cell);
}
// /*cMetaBiologicalObject Cell = */
// //Current3DWorld.ListMetaObjectList[0].AssociateWith(Current3DWorld.ListMetaObjectList[0].FindTheClosestVolumeFrom(TmpSpot.GetCentroid(), 20), TmpSpot);
}
// if (Obj.radioButtonColorSingle.Checked)
// Current3DWorld.AddBiological3DObjects(Obj.AssociatedBiologicalObjectList, Obj.buttonChangeColorPositive.BackColor, eColorMode.Regular);
// else if (Obj.radioButtonColorRandom.Checked)
// Current3DWorld.AddBiological3DObjects(Obj.AssociatedBiologicalObjectList, Obj.buttonChangeColorPositive.BackColor, eColorMode.Random);
// else if (Obj.radioButtonColorIndexed.Checked)
// Current3DWorld.AddBiological3DObjects(Obj.AssociatedBiologicalObjectList, Obj.buttonChangeColorPositive.BackColor, eColorMode.Indexed);
}
else if (Obj.radioButtonIsVolumeRendering.Checked)
{
Current3DWorld.AddVolume3D(new cVolumeRendering3D(this.AssociatedImage.SingleChannelImage[(int)Obj.numericUpDownChannel.Value],
new cPoint3D((float)Obj.WindowForPreProcessing.numericUpDownShiftX.Value, (float)Obj.WindowForPreProcessing.numericUpDownShiftY.Value, (float)Obj.WindowForPreProcessing.numericUpDownShiftZ.Value),
null, Current3DWorld));
// cVolume3D Volume = new cVolume3D(SeqToAnalyse, (int)Obj.numericUpDownChannel.Value, new cPoint3D((float)Obj.WindowForPreProcessing.numericUpDownShiftX.Value, (float)Obj.WindowForPreProcessing.numericUpDownShiftY.Value, (float)Obj.WindowForPreProcessing.numericUpDownShiftZ.Value));
// Current3DWorld.AddVolume3D(Volume, Color.Black, Obj.buttonChangeColorPositive.BackColor);
}
// Thread oThread = new Thread(new ThreadStart(Obj.DisplayDone));
}
#endregion
#region Objects Association
cMetaBiologicalObjectList MetaCellList = Current3DWorld.ListMetaObjectList[0];
// let's associate the objects
for (int k = 0; k < ListFormForControl.Count; k++)
{
FormForControl Obj = ListFormForControl.ElementAt(ListFormForControl.Count - k -1);
// that's a volume rendering ... no connection
if (Obj.radioButtonIsVolumeRendering.Checked) continue;
// the object is the master
if (Obj.IsMasterObject()) continue;
// this object has not connections ... is not associated to the master
if (Obj.comboBoxLinkToTheMaster.SelectedIndex == 0) continue;
cPoint3D ClosestPt = new cPoint3D(0, 0, 0);
foreach (cBiological3DVolume CurrentSubObj in Obj.AssociatedBiologicalObjectList)
{
cBiological3DVolume ObjectToIdentify = null;
switch (Obj.comboBoxLinkToTheMaster.SelectedIndex)
{
case 1: // association by distance to the master centroid
ObjectToIdentify = MetaCellList.FindTheClosestVolumeCentroidFrom(CurrentSubObj.GetCentroid(), (double)Obj.WindowDistanceToMaster.numericUpDownDistanceMaxToMaster.Value);
break;
case 2:
// //cBiological3DVolume CurrentVolume
ObjectToIdentify = MetaCellList.FindTheClosestVolumeFrom(CurrentSubObj, (double)Obj.WindowDistanceToMaster.numericUpDownDistanceMaxToMaster.Value, out ClosestPt);
break;
default:
break;
}
if (ObjectToIdentify == null) continue;
cMetaBiologicalObject Cell = MetaCellList.AssociateWith(ObjectToIdentify, CurrentSubObj);
// // -------------- draw information and links between the objects ---------------------
if (Obj.WindowDistanceToMaster.checkBoxDrawLinkToMasterCenter.Checked)
{
c3DLine CurrLine = new c3DLine(CurrentSubObj.GetCentroid(), ObjectToIdentify.GetCentroid());
Current3DWorld.AddGeometric3DObject(CurrLine);
//if (Obj.WindowDistanceToMaster.checkBoxDisplayBranchToCenterDistance.Checked)
// CurrLine.DisplayLenght(Current3DWorld, 0.4);
}
// // -------------- draw information and links between the objects ---------------------
if (Obj.WindowDistanceToMaster.checkBoxDrawLinkToMasterEdges.Checked)
{
c3DLine CurrLine1 = new c3DLine(CurrentSubObj.GetCentroid(), ClosestPt);
Current3DWorld.AddGeometric3DObject(CurrLine1);
//if(Obj.WindowDistanceToMaster.checkBoxDisplayBranchToEdgesDistance.Checked)
// CurrLine1.DisplayLenght(Current3DWorld, 0.4);
}
}
}
#endregion
#region Post Processings
for (int k = 0; k < ListFormForControl.Count; k++)
{
FormForControl Obj = ListFormForControl.ElementAt(ListFormForControl.Count - k - 1);
// the object is the master
// // if (!Obj.IsMasterObject()) continue;
if (!Obj.WindowForMaster.checkBoxDrawAssociatedDelaunay.Checked) continue;
foreach (cMetaBiologicalObject CurrentMeta in MetaCellList)
{
Current3DWorld.AddGeometric3DObject(CurrentMeta.GenerateDelaunay(2.0f,true));
}
}
if (PostProcessWindow.checkBoxRemoveUnAssociatedObjects.Checked)
{
int RemovedObjects = Current3DWorld.RemoveNonAssociatedObjects();
Console.WriteLine(RemovedObjects + " objects removed.");
}
// if (PostProcessWindow.checkBoxExportMetaObjectSignatures.Checked)
// {
// if (CurrentTable == null)
// {
// CurrentTable = new DataTable();
// if (CurrentExperiment == null)
// CurrentTable.Columns.Add(new DataColumn("Image Idx", typeof(int)));
// else
// {
// CurrentTable.Columns.Add(new DataColumn("Image Col.", typeof(int)));
// CurrentTable.Columns.Add(new DataColumn("Image Row", typeof(int)));
// }
// CurrentTable.Columns.Add(new DataColumn("Meta Object Name", typeof(string)));
// foreach (string DescName in MetaCellList[0].GetSignatureNames())
// CurrentTable.Columns.Add(new DataColumn(DescName, typeof(double)));
// CurrentTable.Columns.Add(new DataColumn("Class", typeof(double)));
// }
// foreach (cMetaBiologicalObject CurrentMeta in MetaCellList)
// {
// List<double> CurrentSignature = CurrentMeta.GetSignature();
// CurrentTable.Rows.Add();
// if (CurrentExperiment == null)
// {
// CurrentTable.Rows[CurrentTable.Rows.Count - 1][0] = IdxImageProcessed;
// CurrentTable.Rows[CurrentTable.Rows.Count - 1][1] = CurrentMeta.Name;
// for (int Idx = 0; Idx < CurrentSignature.Count; Idx++)
// CurrentTable.Rows[CurrentTable.Rows.Count - 1][Idx + 2] = CurrentSignature[Idx];
// }
// else
// {
// CurrentTable.Rows[CurrentTable.Rows.Count - 1][0] = CurrentExperiment.Column;
// CurrentTable.Rows[CurrentTable.Rows.Count - 1][1] = CurrentExperiment.Row;
// CurrentTable.Rows[CurrentTable.Rows.Count - 1][2] = CurrentMeta.Name;
// for (int Idx = 0; Idx < CurrentSignature.Count; Idx++)
// CurrentTable.Rows[CurrentTable.Rows.Count - 1][Idx + 3] = CurrentSignature[Idx];
// }
// // Current3DWorld.CopyMetaObjectSignatureToTable(CurrentMeta, 0);
// }
// if (DataGrid != null)
// {
// DataGrid.dataGridViewForResults.DataSource = CurrentTable;
// DataGrid.Update();
// IdxImageProcessed++;
// }
// }
#endregion
// //CurrentTable = new DataTable();
// if (CurrentExperiment == null)
// {
// if (PostProcessWindow.checkBoxDisplayBottomPlate.Checked) Current3DWorld.DisplayBottom(Color.FromArgb(255, 255, 255));
// Current3DWorld.SetBackgroundColor(PostProcessWindow.buttonChangeColorPositive.BackColor);
// Current3DWorld.Render();
// }
// // Current3DWorld.SetLinkToDataGridView(dataGridViewForClassif);
cViewer3D V3D = new cViewer3D();
V3D.SetInputData(Current3DWorld);
V3D.Run();
cDisplayToWindow DTW = new cDisplayToWindow();
DTW.SetInputData(V3D.GetOutPut());
DTW.Run();
DTW.Display();
}
public void CopyMetaObjectSignatureToTable(cMetaBiologicalObject MetaObject, int ObjectClass)
{
List<string> Descriptors = MetaObject.Information.GetGlobalDescriptorNames();
cInteractive3DObject MasterObj = MetaObject.GetMasterObject();
List<double> ListDescMaster = null;
List<string> ListProfilName = null;
cObject3D Master3d = (cObject3D)(MasterObj);
if (Master3d.GetType().ToString().IndexOf("cBiological3DVolume") != -1)
{
cBiological3DVolume TmpVol = (cBiological3DVolume)Master3d;
ListProfilName = TmpVol.Information.GetDescriptorNames();
ListDescMaster = TmpVol.Information.GetInformation();
}
else if (Master3d.GetType().ToString().IndexOf("cBiologicalSpot") != -1)
{
cBiologicalSpot TmpSpot = (cBiologicalSpot)Master3d;
ListProfilName = TmpSpot.Information.GetDescriptorNames();
ListDescMaster = TmpSpot.Information.GetInformation();
}
if ((CurrentTable == null) || (CurrentTable.Columns.Count == 0) || (CurrentTable.Rows.Count == 0))
{
CurrentTable = new DataTable();
//cBiological3DObject MasterObj = MetaObject.GetMasterObject();
CurrentTable.Columns.Add(new DataColumn("Meta Object Name", typeof(string)));
foreach (string DescName in ListProfilName)
CurrentTable.Columns.Add(new DataColumn(DescName, typeof(double)));
for (int iDesc = 0; iDesc < Descriptors.Count; iDesc++)
CurrentTable.Columns.Add(new DataColumn(Descriptors[iDesc], typeof(double)));
//for (int iDesc = 0; iDesc < Descriptors.Count; iDesc++)
// CurrentTable.Columns.Add(new DataColumn(Descriptors[iDesc], typeof(double)));
CurrentTable.Columns.Add(new DataColumn("Class", typeof(double)));
if (pDataGridView == null) Console.WriteLine("c3Dworld does not contains the link to the datagridview control");
}
List<double> Res = MetaObject.Information.GetInformation(Descriptors);
List<object> objectNumbers = new List<object>();
if (Res == null) return;
foreach (double number in Res)
objectNumbers.Add((object)number);
objectNumbers.Add(ObjectClass);
CurrentTable.Rows.Add();
CurrentTable.Rows[CurrentTable.Rows.Count - 1][0] = MetaObject.Name;
for (int Idx = 0; Idx < ListDescMaster.Count; Idx++)
CurrentTable.Rows[CurrentTable.Rows.Count - 1][Idx + 1] = ListDescMaster[Idx];
for (int Idx = 0; Idx <= Res.Count; Idx++)
CurrentTable.Rows[CurrentTable.Rows.Count - 1][Idx + ListDescMaster.Count + 1] = objectNumbers[Idx];
if (pDataGridView != null)
{
pDataGridView.DataSource = CurrentTable;
pDataGridView.Update();
}
}
internal void DefineMetaContainer(cMetaBiologicalObject cMetaBiologicalObjectContainer)
{
this.MetaObjectContainer = cMetaBiologicalObjectContainer;
}
public void DisplayXYZ()
{
if (CompleteScreening == null) return;
int DescX = this.comboBoxDescriptorX.SelectedIndex;
int DescY = this.comboBoxDescriptorY.SelectedIndex;
int DescZ = this.comboBoxDescriptorZ.SelectedIndex;
if (DescX < 0) DescX = 0;
if (DescY < 0) DescY = 0;
if (DescZ < 0) DescZ = 0;
int[] Pos = new int[2];
Pos[0] = 0;
Pos[1] = 0;
if (CurrentWorld == null)
{
CurrentWorld = new c3DWorld(new cPoint3D(1000, 1000, 1000), new cPoint3D(ListScales[DescX], ListScales[DescY], ListScales[DescZ]), this.renderWindowControl1, Pos, CompleteScreening);
}
CurrentWorld.SetBackgroundColor(Color.Black);
CurrentWorld.ren1.RemoveAllViewProps();
// if (widget != null) widget.SetEnabled(0);
Series CurrentSeries = new Series("ScatterPoints");
double MinX = double.MaxValue;
double MinY = double.MaxValue;
double MinZ = double.MaxValue;
double MaxZ = double.MinValue;
double MaxX = double.MinValue;
double MaxY = double.MinValue;
double TempX, TempY, TempZ;
int Idx = 0;
cExtendPlateList ListPlate = new cExtendPlateList();
cMetaBiologicalObjectList ListMeta = new cMetaBiologicalObjectList("Test");
cBiologicalSpot CurrentSpot1 = new cBiologicalSpot(Color.White, new cPoint3D(0, 0, 0), 1, 4);
cMetaBiologicalObject Plate3D = new cMetaBiologicalObject("Data", ListMeta, CurrentSpot1);
if (!IsFullScreen)
ListPlate.Add(CompleteScreening.GetCurrentDisplayPlate());
else
ListPlate = CompleteScreening.ListPlatesActive;
vtkUnsignedCharArray colors = vtkUnsignedCharArray.New();
colors.SetName("colors");
colors.SetNumberOfComponents(3);
vtkPoints Allpoints = vtkPoints.New();
cExtendedList ListPtX = new cExtendedList();
cExtendedList ListPtY = new cExtendedList();
cExtendedList ListPtZ = new cExtendedList();
for (int i = 0; i < ListPlate.Count; i++)
{
cPlate CurrentPlate = ListPlate[i];
for (int IdxValue = 0; IdxValue < CompleteScreening.Columns; IdxValue++)
for (int IdxValue0 = 0; IdxValue0 < CompleteScreening.Rows; IdxValue0++)
{
cWell TmpWell = CurrentPlate.GetWell(IdxValue, IdxValue0, true);
if (TmpWell != null)
{
TempX = TmpWell.ListDescriptors[DescX].GetValue();
if (TempX < MinX) MinX = TempX;
if (TempX > MaxX) MaxX = TempX;
TempY = TmpWell.ListDescriptors[DescY].GetValue();
if (TempY < MinY) MinY = TempY;
if (TempY > MaxY) MaxY = TempY;
TempZ = TmpWell.ListDescriptors[DescZ].GetValue();
if (TempZ < MinZ) MinZ = TempZ;
if (TempZ > MaxZ) MaxZ = TempZ;
// cBiologicalSpot CurrentSpot = new cBiologicalSpot(TmpWell.GetColor(), new cPoint3D(TempX, TempY, TempZ), 1, 4);
List<char> Col = new List<char>();
Col.Add((char)(TmpWell.GetColor().R));
Col.Add((char)(TmpWell.GetColor().G));
Col.Add((char)(TmpWell.GetColor().B));
// IntPtr unmanagedPointer = Marshal.UnsafeAddrOfPinnedArrayElement(Col.ToArray(), 0);
//colors.InsertNextTupleValue(unmanagedPointer);
colors.InsertNextTuple3(Col[0], Col[1], Col[2]);
ListPtX.Add(TempX);
ListPtY.Add(TempY);
ListPtZ.Add(TempZ);
// CurrentSpot.Name = TmpWell.AssociatedPlate.Name + " - " + TmpWell.GetPosX() + "x" + TmpWell.GetPosY() + " :" + TmpWell.Name;
// CurrentSpot.ObjectType = TmpWell.AssociatedPlate.Name + " - " + TmpWell.GetPosX() + "x" + TmpWell.GetPosY() + " :" + TmpWell.Name;
// Plate3D.AddObject(CurrentSpot);
// CurrentWorld.AddBiological3DObject(CurrentSpot);
//CurrentSeries.Points.Add(TempX, TempY);
// if (IsFullScreen)
// CurrentSeries.Points[Idx].ToolTip = TmpWell.AssociatedPlate.Name + "\n" + TmpWell.GetPosX() + "x" + TmpWell.GetPosY() + " :" + TmpWell.Name;
// else
// CurrentSeries.Points[Idx].ToolTip = TmpWell.GetPosX() + "x" + TmpWell.GetPosY() + " :" + TmpWell.Name;
Idx++;
}
}
}
double MinValueX = ListPtX.Min();
double MaxValueX = ListPtX.Max();
cExtendedList NormX = ListPtX.Normalize(MinValueX, MaxValueX);
double MinValueY = ListPtY.Min();
double MaxValueY = ListPtY.Max();
cExtendedList NormY = ListPtY.Normalize(MinValueY, MaxValueY);
double MinValueZ = ListPtZ.Min();
double MaxValueZ = ListPtZ.Max();
cExtendedList NormZ = ListPtZ.Normalize(MinValueZ, MaxValueZ);
for (int IdxPt = 0; IdxPt < ListPtX.Count; IdxPt++)
Allpoints.InsertNextPoint(NormX[IdxPt], NormY[IdxPt], NormZ[IdxPt]);
vtkPolyData polydata = vtkPolyData.New();
polydata.SetPoints(Allpoints);
polydata.GetPointData().SetScalars(colors);
vtkSphereSource SphereSource = vtkSphereSource.New();
SphereSource.SetRadius(RadiusSphere);
vtkGlyph3D glyph3D = vtkGlyph3D.New();
glyph3D.SetColorModeToColorByScalar();
glyph3D.SetSourceConnection(SphereSource.GetOutputPort());
glyph3D.SetInput(polydata);
glyph3D.ScalingOff();
glyph3D.Update();
vtkPolyDataMapper mapper = vtkPolyDataMapper.New();
mapper.SetInputConnection(glyph3D.GetOutputPort());
vtkActor actor = vtkActor.New();
actor.SetMapper(mapper);
CurrentWorld.ren1.AddActor(actor);
if ((CompleteScreening.GetCurrentDisplayPlate().ListDRCRegions != null) && (CompleteScreening.GlobalInfo.OptionsWindow.checkBoxConnectDRCPts.Checked))
{
foreach (cDRC_Region TmpRegion in CompleteScreening.GetCurrentDisplayPlate().ListDRCRegions)
{
int cpt = 0;
cWell[][] ListWells = TmpRegion.GetListWells();
foreach (cWell[] item in ListWells)
{
for (int IdxWell = 0; IdxWell < item.Length - 1; IdxWell++)
{
//cWell TmpWell0 = CompleteScreening.GetCurrentDisplayPlate().GetWell(item[IdxWell], IdxValue0, true);
if ((item[IdxWell] != null) && (item[IdxWell + 1] != null) && (item[IdxWell].GetClass() >= -1))
{
double StartX = (item[IdxWell].ListDescriptors[DescX].GetValue() - MinValueX) / (MaxValueX - MinValueX);
double StartY = (item[IdxWell].ListDescriptors[DescY].GetValue() - MinValueY) / (MaxValueY - MinValueY);
double StartZ = (item[IdxWell].ListDescriptors[DescZ].GetValue() - MinValueZ) / (MaxValueZ - MinValueZ);
double EndX = (item[IdxWell + 1].ListDescriptors[DescX].GetValue() - MinValueX) / (MaxValueX - MinValueX);
double EndY = (item[IdxWell + 1].ListDescriptors[DescY].GetValue() - MinValueY) / (MaxValueY - MinValueY);
double EndZ = (item[IdxWell + 1].ListDescriptors[DescZ].GetValue() - MinValueZ) / (MaxValueZ - MinValueZ);
cPoint3D StartPt = new cPoint3D(StartX, StartY, StartZ);
cPoint3D EndPt = new cPoint3D(EndX, EndY, EndZ);
c3DLine NewLine = new c3DLine(StartPt, EndPt);
CurrentWorld.AddGeometric3DObject(NewLine);
}
}
}
/*List<cDRC> ListDRC = new List<cDRC>();
for (int i = 0; i < CompleteScreening.ListDescriptors.Count; i++)
{
if (CompleteScreening.ListDescriptors[i].IsActive())
{
cDRC CurrentDRC = new cDRC(TmpRegion, CompleteScreening.ListDescriptors[i]);
ListDRC.Add(CurrentDRC);
cpt++;
}
}
*/
//cDRCDisplay DRCDisplay = new cDRCDisplay(ListDRC, GlobalInfo);
//if (DRCDisplay.CurrentChart.Series.Count == 0) continue;
//DRCDisplay.CurrentChart.Location = new Point((DRCDisplay.CurrentChart.Width + 50) * 0, (DRCDisplay.CurrentChart.Height + 10 + DRCDisplay.CurrentRichTextBox.Height) * h++);
//DRCDisplay.CurrentRichTextBox.Location = new Point(DRCDisplay.CurrentChart.Location.X, DRCDisplay.CurrentChart.Location.Y + DRCDisplay.CurrentChart.Height + 5);
//WindowforDRCsDisplay.LChart.Add(DRCDisplay.CurrentChart);
//WindowforDRCsDisplay.LRichTextBox.Add(DRCDisplay.CurrentRichTextBox);
}
}
// vtkAxesActor axis = vtkAxesActor.New();
vtkAxisActor axisX = vtkAxisActor.New();
axisX.SetPoint1(0, 0, 0);
axisX.SetPoint2(1, 0, 0);
axisX.SetTickLocationToBoth();
axisX.SetDeltaMajor(0.1);
axisX.SetMajorTickSize(0);
axisX.MinorTicksVisibleOff();
//axisX.Maj
CurrentWorld.ren1.AddActor(axisX);
vtkAxisActor axisY = vtkAxisActor.New();
axisY.SetPoint1(0, 0, 0);
axisY.SetPoint2(0, 1, 0);
axisY.SetTickLocationToBoth();
axisY.SetDeltaMajor(0.1);
axisY.SetMajorTickSize(0.05);
axisY.MinorTicksVisibleOff();
CurrentWorld.ren1.AddActor(axisY);
vtkAxisActor axisZ = vtkAxisActor.New();
axisZ.SetPoint1(0, 0, 0);
axisZ.SetPoint2(0, 0, 1);
axisZ.SetTickLocationToBoth();
axisZ.SetDeltaMajor(0.1);
axisZ.SetMajorTickSize(0.05);
axisZ.MinorTicksVisibleOff();
CurrentWorld.ren1.AddActor(axisZ);
if (widget == null)
{
widget = vtkOrientationMarkerWidget.New();
axes = vtkAxesActor.New();
widget.SetOutlineColor(0.9300, 0.5700, 0.1300);
widget.SetInteractor(CurrentWorld.iren);
widget.SetViewport(0.0, 0.0, 0.4, 0.4);
widget.SetEnabled(0);
// widget.InteractiveOn();
if (this.comboBoxDescriptorX.SelectedItem == null)
axes.SetXAxisLabelText(this.comboBoxDescriptorX.Items[0].ToString());
else
axes.SetXAxisLabelText(this.comboBoxDescriptorX.SelectedItem.ToString());
if (this.comboBoxDescriptorY.SelectedItem == null)
axes.SetYAxisLabelText(this.comboBoxDescriptorY.Items[0].ToString());
else
axes.SetYAxisLabelText(this.comboBoxDescriptorY.SelectedItem.ToString());
if (this.comboBoxDescriptorZ.SelectedItem == null)
axes.SetZAxisLabelText(this.comboBoxDescriptorZ.Items[0].ToString());
else
axes.SetZAxisLabelText(this.comboBoxDescriptorZ.SelectedItem.ToString());
widget.SetOrientationMarker(axes);
}
else
{
if(this.comboBoxDescriptorX.SelectedItem!=null)
axes.SetXAxisLabelText(this.comboBoxDescriptorX.SelectedItem.ToString());
if (this.comboBoxDescriptorY.SelectedItem != null)
axes.SetYAxisLabelText(this.comboBoxDescriptorY.SelectedItem.ToString());
if (this.comboBoxDescriptorZ.SelectedItem != null)
axes.SetZAxisLabelText(this.comboBoxDescriptorZ.SelectedItem.ToString());
widget.SetOrientationMarker(axes);
}
//
//vtkCameraWidget Wid = vtkCameraWidget.New();
//Wid.SetInteractor(CurrentWorld.iren);
//Wid.SetEnabled(1);
// Wid.InteractiveOn();
//vtkDistanceWidget distanceWidget = vtkDistanceWidget.New();
//distanceWidget.SetInteractor(CurrentWorld.iren);
//distanceWidget.SetEnabled(1);
//distanceWidget.CreateDefaultRepresentation();
//((vtkDistanceRepresentation)distanceWidget.GetRepresentation()).SetLabelFormat("%-#6.3g mm");
/*static_cast<vtkDistanceRepresentation*>(distanceWidget->GetRepresentation())
->SetLabelFormat("%-#6.3g mm");
*/
// Plate3D.GenerateAndDisplayBoundingBox(1, Color.White, false, CurrentWorld);
//c3DText CaptionX = new c3DText(CurrentWorld, CompleteScreening.ListDescriptors[DescX].GetName(), new cPoint3D(MaxX, MinY, MinZ), Color.DarkRed, this.FontSize);
//c3DLine LineX = new c3DLine(new cPoint3D(MinX, MinY, MinZ), new cPoint3D(MaxX, MinY, MinZ), Color.DarkRed);
//CurrentWorld.AddGeometric3DObject(LineX);
//c3DText CaptionY = new c3DText(CurrentWorld, CompleteScreening.ListDescriptors[DescY].GetName(), new cPoint3D(MinX, MaxY, MinZ), Color.DarkGreen, this.FontSize);
//c3DLine LineY = new c3DLine(new cPoint3D(MinX, MinY, MinZ), new cPoint3D(MinX, MaxY, MinZ), Color.DarkGreen);
//CurrentWorld.AddGeometric3DObject(LineY);
//c3DText CaptionZ = new c3DText(CurrentWorld, CompleteScreening.ListDescriptors[DescZ].GetName(), new cPoint3D(MinX, MinY, MaxZ), Color.DarkBlue, this.FontSize);
//c3DLine LineZ = new c3DLine(new cPoint3D(MinX, MinY, MinZ), new cPoint3D(MinX, MinY, MaxZ), Color.DarkBlue);
//CurrentWorld.AddGeometric3DObject(LineZ);
CurrentWorld.SimpleRender();// Render();
}
public void Display3Dplate(int IdxDescriptor, cPoint3D MinimumPosition)
{
ParentScreening._3DWorldForPlateDisplay.ListMetaObjectList = new List<cMetaBiologicalObjectList>();
cMetaBiologicalObjectList ListMetacells = new cMetaBiologicalObjectList("List Meta Objects");
ParentScreening._3DWorldForPlateDisplay.ListMetaObjectList.Add(ListMetacells);
c3DWell NewPlate3D = new c3DWell(new cPoint3D(0, 0, 0), new cPoint3D(ParentScreening.Columns, ParentScreening.Rows, 1), Color.Black, null);
NewPlate3D.SetOpacity(0.0);
cMetaBiologicalObject Plate3D = new cMetaBiologicalObject(this.Name, ListMetacells, NewPlate3D);
#region display the well list
foreach (cWell TmpWell in this.ListWell)
{
if ((TmpWell == null) || (TmpWell.GetCurrentClassIdx() == -1)) continue;
double PosZ = 8 - ((TmpWell.ListSignatures[IdxDescriptor].GetValue() - this.ListMinMax[IdxDescriptor][0]) * 8) / (this.ListMinMax[IdxDescriptor][1] - this.ListMinMax[IdxDescriptor][0]);
double WellSize = (double)cGlobalInfo.OptionsWindow.numericUpDownWellSize.Value;
double WellBorder = (1 - WellSize) / 2.0;
cPoint3D CurrentPos = new cPoint3D(TmpWell.GetPosX() - WellBorder + MinimumPosition.X, TmpWell.GetPosY() + WellBorder + MinimumPosition.Y - WellSize / 2, PosZ + MinimumPosition.Z - WellBorder);
Color WellColor = Color.Black;
if (cGlobalInfo.IsDisplayClassOnly)
WellColor = TmpWell.GetClassColor();
else
{
int ConvertedValue;
byte[][] LUT = cGlobalInfo.CurrentPlateLUT;
if (this.ListMinMax[IdxDescriptor][0] == this.ListMinMax[IdxDescriptor][1])
ConvertedValue = 0;
else
ConvertedValue = (int)(((TmpWell.ListSignatures[IdxDescriptor].GetValue() - this.ListMinMax[IdxDescriptor][0]) * (LUT[0].Length - 1)) / (this.ListMinMax[IdxDescriptor][1] - this.ListMinMax[IdxDescriptor][0]));
if ((ConvertedValue >= 0) && (ConvertedValue < LUT[0].Length))
WellColor = Color.FromArgb(LUT[0][ConvertedValue], LUT[1][ConvertedValue], LUT[2][ConvertedValue]);
}
c3DWell New3DWell = new c3DWell(CurrentPos, new cPoint3D(CurrentPos.X + WellSize, CurrentPos.Y + WellSize, CurrentPos.Z + WellSize / 2.0), WellColor, TmpWell);
New3DWell.SetType("[" + TmpWell.GetPosX() + " x " + TmpWell.GetPosY() + "]");
cPropertyType WellPropertyType = null;
foreach (object item in cGlobalInfo.WindowHCSAnalyzer.toolStripDropDownButtonDisplayMode.DropDownItems)
{
if (item.GetType() == typeof(ToolStripMenuItem))
{
ToolStripMenuItem TmpMenuItem = ((ToolStripMenuItem)item);
if (TmpMenuItem.Checked && (TmpMenuItem.Tag != null) && (TmpMenuItem.Tag.GetType() == typeof(cPropertyType)))
{
WellPropertyType = ((cPropertyType)(TmpMenuItem).Tag);
break;
}
}
}
if ((WellPropertyType != null) && (cGlobalInfo.ViewMode != eViewMode.PIE))
{
Title MainLegend = new Title();
string ToDisp = "";
if (WellPropertyType != null)
{
object CurrentValue = TmpWell.ListProperties.FindValueByName(WellPropertyType.Name);
if (CurrentValue != null) ToDisp = CurrentValue.ToString();//((double)CurrentValue).ToString("e4");
else
ToDisp = "n.a.";
}
New3DWell.AddText(ToDisp, ParentScreening._3DWorldForPlateDisplay, 0.1);
}
New3DWell.SetOpacity((double)cGlobalInfo.OptionsWindow.numericUpDownWellOpacity.Value);
ParentScreening._3DWorldForPlateDisplay.AddBiological3DObject(New3DWell);
Plate3D.AddObject(New3DWell);
}
#endregion
#region Well numbering
if (cGlobalInfo.OptionsWindow.checkBox3DPlateInformation.Checked)
{
for (int i = 1; i <= ParentScreening.Columns; i++)
{
c3DText CurrentText = new c3DText(ParentScreening._3DWorldForPlateDisplay, i.ToString(), new cPoint3D(i - 1 + MinimumPosition.X, -0.5 + MinimumPosition.Y, 0 + MinimumPosition.Z), Color.White, 0.35);
ParentScreening._3DWorldForPlateDisplay.AddGeometric3DObject(CurrentText);
}
for (int j = 1; j <= ParentScreening.Rows; j++)
{
c3DText CurrentText1;
if (cGlobalInfo.OptionsWindow.radioButtonWellPosModeDouble.Checked)
CurrentText1 = new c3DText(ParentScreening._3DWorldForPlateDisplay, j.ToString(), new cPoint3D(-1 + MinimumPosition.X, j - 0.5 + MinimumPosition.Y, 0 + MinimumPosition.Z), Color.White, 0.35);
else
CurrentText1 = new c3DText(ParentScreening._3DWorldForPlateDisplay, cGlobalInfo.ConvertIntPosToStringPos(j), new cPoint3D(-1 + MinimumPosition.X, j - 0.5 + MinimumPosition.Y, 0 + MinimumPosition.Z), Color.White, 0.35);
ParentScreening._3DWorldForPlateDisplay.AddGeometric3DObject(CurrentText1);
}
}
#endregion
if (this.ListDRCRegions != null)
{
foreach (cDRC_Region Region in this.ListDRCRegions)
{
if (cGlobalInfo.OptionsWindow.checkBox1.Checked)
{
cDRC CurrentDRC = Region.GetDRC(this.ParentScreening.ListDescriptors[IdxDescriptor]);
if ((CurrentDRC == null) || (CurrentDRC.ResultFit == null) || (CurrentDRC.ResultFit.Y_Estimated.Count <= 1)) continue;
c3DDRC New3DDRC = new c3DDRC(CurrentDRC, Region, Color.White, this.ListMinMax[IdxDescriptor][0], this.ListMinMax[IdxDescriptor][1]);
New3DDRC.SetOpacity((double)cGlobalInfo.OptionsWindow.numericUpDownDRCOpacity.Value);
//New3DDRC.SetType("[" + TmpWell.GetPosX() + " x " + TmpWell.GetPosY() + "]");
ParentScreening._3DWorldForPlateDisplay.AddGeometric3DObject(New3DDRC);
//Plate3D.AddObject(New3DDRC);
}
if (cGlobalInfo.OptionsWindow.checkBox3DComputeThinPlate.Checked)
{
c3DThinPlate NewThinPlate = new c3DThinPlate(Region, (double)cGlobalInfo.OptionsWindow.numericUpDown3DThinPlateRegularization.Value);
if (cGlobalInfo.OptionsWindow.checkBox3DDisplayThinPlate.Checked)
{
NewThinPlate.SetOpacity(0.5);
NewThinPlate.SetToWireFrame();
ParentScreening._3DWorldForPlateDisplay.AddGeometric3DObject(NewThinPlate);
}
if (cGlobalInfo.OptionsWindow.checkBox3DDisplayIsoboles.Checked)
{
for (double PosZContour = 0; PosZContour <= 10.0; PosZContour += 1.5)
{
c3DIsoContours NewContour = new c3DIsoContours(NewThinPlate, Region, Color.Red, PosZContour, true);
ParentScreening._3DWorldForPlateDisplay.AddBiological3DObject(NewContour);
}
}
if (cGlobalInfo.OptionsWindow.checkBox3DDisplayIsoRatioCurves.Checked)
{
// for (double PosZContour = 0; PosZContour <= 15.0; PosZContour += 3)
{
c3DIsoContours NewContour = new c3DIsoContours(NewThinPlate, Region, Color.Blue, 0/*PosZContour*/, false);
ParentScreening._3DWorldForPlateDisplay.AddBiological3DObject(NewContour);
}
}
}
}
}
}
