Exemplo n.º 1
0
        public void Histogram()
        {
            int[] tmp = new int[] {
                6, 34, 1, 7, 2, 10, 20, 25, 30, 35, 40, 9, 19, 24, 29, 34, 39, 40, 2, 14, 21, 27, 32, 36, 40
                , 7, 0, 90, 4, 1, 0, 0, 0, 0, 0, -70, 22, 4, 0, -81, -49, 1, 0, -68, 16, 0, 0, 78, -86, 42, 0, 0, 0, 0, 0, 0, 0, 0, 0
                , 7, 0, 90, 4, 2, 0, 0, 0, 0, 0, 75, -81, 3, 0, -96, -81, 0, 0, 76, 4, 0, 0, 112, 62, 44, 0, 0, 0, 0, 0, 0, 0, 0, 0
                , 7, 0, 90, 4, 3, 0, 0, 0, 0, 0, -80, 60, 3, 0, -74, -46, 0, 0, -85, 1, 0, 0, -106, -112, 44, 0, 0, 0, 0, 0, 0, 0, 0, 0
            };
            byte[] expected_binary_data = new byte[tmp.Length];
            for (int i = 0; i < expected_binary_data.Length; i++)
            {
                expected_binary_data[i] = (byte)tmp[i];
            }
            run_metrics run = new run_metrics();

            c_csharp_comm.read_interop_from_buffer(expected_binary_data, (uint)expected_binary_data.Length, run.q_metric_set());

            filter_options   options = new filter_options(tile_naming_method.FourDigit);
            read_info_vector reads   = new read_info_vector();

            reads.Add(new read_info(1, 1, 26));
            reads.Add(new read_info(2, 27, 76));
            run.run_info(new info(
                             new flowcell_layout(8, 2, 2, 36, 1, 1, new string_vector(), tile_naming_method.FourDigit),
                             reads
                             ));
            run.legacy_channel_update(instrument_type.HiSeq);
            run.finalize_after_load();

            bar_plot_data data = new bar_plot_data();

            c_csharp_plot.plot_qscore_histogram(run, options, data);
            Assert.AreEqual(data.size(), 1);
        }
Exemplo n.º 2
0
		public void Histogram()
		{

			int[] tmp = new int[]{
                6,34,1,7,2,10,20,25,30,35,40,9,19,24,29,34,39,40,2,14,21,27,32,36,40
                ,7,0,90,4,1,0,0,0,0,0,-70,22,4,0,-81,-49,1,0,-68,16,0,0,78,-86,42,0,0,0,0,0,0,0,0,0
                ,7,0,90,4,2,0,0,0,0,0,75,-81,3,0,-96,-81,0,0,76,4,0,0,112,62,44,0,0,0,0,0,0,0,0,0
                ,7,0,90,4,3,0,0,0,0,0,-80,60,3,0,-74,-46,0,0,-85,1,0,0,-106,-112,44,0,0,0,0,0,0,0,0,0
			};
            byte[] expected_binary_data = new byte[tmp.Length];
            for(int i=0;i<expected_binary_data.Length;i++) expected_binary_data[i] = (byte)tmp[i];
            run_metrics run = new run_metrics();
            c_csharp_comm.read_interop_from_buffer(expected_binary_data, (uint)expected_binary_data.Length, run.q_metric_set());

            filter_options options = new filter_options(tile_naming_method.FourDigit);
            read_info_vector reads = new read_info_vector();
            reads.Add(new read_info(1, 1, 26));
            reads.Add(new read_info(2, 27, 76));
            run.run_info(new info(
                    "",
                    "",
                    1,
                    new flowcell_layout(8, 2, 2, 36, 1, 1, new string_vector(), tile_naming_method.FourDigit),
                    new string_vector(),
                    new image_dimensions(),
                    reads
            ));
            run.legacy_channel_update(instrument_type.HiSeq);
            run.finalize_after_load();

            bar_plot_data data = new bar_plot_data();
            c_csharp_plot.plot_qscore_histogram(run, options, data);
            Assert.AreEqual(data.size(), 1);

		}
Exemplo n.º 3
0
        public void ReadsIdentified()
        {
            int[] tmp1 = new int[] { 1
                                     , 1, 0, 74, 47, 3, 0
                                     , 17, 0, 65, 84, 67, 65, 67, 71, 65, 67, 45, 65, 65, 71, 71, 84, 84, 67, 65, 218, 17, 0, 0, 1, 0, 49, 11, 0, 84, 83, 67, 65, 73, 110
                                     , 100, 101, 120, 101, 115
                                     , 2, 0, 74, 47, 3, 0 // changed to lane 2
                                     , 17, 0, 65, 67, 65, 71, 84, 71, 71, 84, 45, 65, 65, 71, 71, 84, 84, 67, 65, 125, 17, 0, 0, 1, 0, 50, 11, 0, 84, 83, 67, 65, 73, 110
                                     , 100, 101, 120, 101, 115
                                     , 3, 0, 74, 47, 3, 0 // changed to lane 3
                                     , 17, 0, 67, 65, 71, 65, 84, 67, 67, 65, 45, 65, 65, 71, 71, 84, 84, 67, 65, 226, 17, 0, 0, 1, 0, 51, 11, 0, 84, 83, 67, 65, 73, 110
                                     , 100, 101, 120, 101, 115 };
            int[] tmp2 = new int[] { 2, 10
                                     , 7, 0, 90, 4, 100, 0, -67, -66, 15, 74
                                     , 7, 0, 90, 4, 102, 0, 74, 122, -59, 74
                                     , 7, 0, 90, 4, 101, 0, 12, 94, -115, 73
                                     , 7, 0, 90, 4, 103, 0, 16, 54, 66, 74
                                     , 7, 0, 90, 4, -56, 0, 82, -11, 80, 58
                                     , 7, 0, 90, 4, -55, 0, -62, 42, -99, 58
                                     , 7, 0, 90, 4, 44, 1, -102, 113, 39, 64
                                     , 7, 0, -66, 4, 100, 0, -67, -66, 15, 74
                                     , 7, 0, -66, 4, 102, 0, 74, 122, -59, 74
                                     , 7, 0, -66, 4, 101, 0, 46, 103, -113, 73
                                     , 7, 0, -66, 4, 103, 0, 0, 2, 69, 74
                                     , 7, 0, -66, 4, -56, 0, 21, 111, -87, 58
                                     , 7, 0, -66, 4, -55, 0, -86, 29, -79, 58
                                     , 7, 0, -66, 4, 44, 1, 6, -42, 39, 64
                                     , 7, 0, 66, 8, 100, 0, -67, -66, 15, 74
                                     , 7, 0, 66, 8, 102, 0, 74, 122, -59, 74
                                     , 7, 0, 66, 8, 101, 0, 67, -26, -109, 73
                                     , 7, 0, 66, 8, 103, 0, 92, 47, 75, 74
                                     , 7, 0, 66, 8, -56, 0, 123, 22, -100, 58
                                     , 7, 0, 66, 8, -55, 0, 85, 6, 115, 58
                                     , 7, 0, 66, 8, 44, 1, 57, 97, 31, 64 };
            byte[] expected_binary_data1 = new byte[tmp1.Length];
            for (int i = 0; i < expected_binary_data1.Length; i++)
            {
                expected_binary_data1[i] = (byte)tmp1[i];
            }
            byte[] expected_binary_data2 = new byte[tmp2.Length];
            for (int i = 0; i < expected_binary_data2.Length; i++)
            {
                expected_binary_data2[i] = (byte)tmp2[i];
            }
            run_metrics run = new run_metrics();

            c_csharp_comm.read_interop_from_buffer(expected_binary_data1, (uint)expected_binary_data1.Length, run.index_metric_set());
            c_csharp_comm.read_interop_from_buffer(expected_binary_data2, (uint)expected_binary_data2.Length, run.tile_metric_set());

            read_info_vector reads = new read_info_vector();

            reads.Add(new read_info(1, 1, 26));
            reads.Add(new read_info(2, 27, 76));
            run.run_info(new info(
                             new flowcell_layout(2, 2, 2, 16),
                             reads
                             ));
            run.legacy_channel_update(instrument_type.HiSeq);

            bar_plot_data data = new bar_plot_data();

            c_csharp_plot.plot_sample_qc(run, 1, data);
            Assert.AreEqual(data.size(), 1);
        }
Exemplo n.º 4
0
		public void ReadsIdentified()
		{

			int[] tmp1 = new int[]{1
                          ,1,0,74,47,3,0
                          ,17,0,65,84,67,65,67,71,65,67,45,65,65,71,71,84,84,67,65,218,17,0,0,1,0,49,11,0,84,83,67,65,73,110
                          ,100,101,120,101,115
                          ,2,0,74,47,3,0 // changed to lane 2
                          ,17,0,65,67,65,71,84,71,71,84,45,65,65,71,71,84,84,67,65,125,17,0,0,1,0,50,11,0,84,83,67,65,73,110
                          ,100,101,120,101,115
                          ,3,0,74,47,3,0 // changed to lane 3
                          ,17,0,67,65,71,65,84,67,67,65,45,65,65,71,71,84,84,67,65,226,17,0,0,1,0,51,11,0,84,83,67,65,73,110
                          ,100,101,120,101,115
            			};
            int[] tmp2 = new int[]{2,10
                        ,7,0,90,4,100,0,-67,-66,15,74
                        ,7,0,90,4,102,0,74,122,-59,74
                        ,7,0,90,4,101,0,12,94,-115,73
                        ,7,0,90,4,103,0,16,54,66,74
                        ,7,0,90,4,-56,0,82,-11,80,58
                        ,7,0,90,4,-55,0,-62,42,-99,58
                        ,7,0,90,4,44,1,-102,113,39,64
                        ,7,0,-66,4,100,0,-67,-66,15,74
                        ,7,0,-66,4,102,0,74,122,-59,74
                        ,7,0,-66,4,101,0,46,103,-113,73
                        ,7,0,-66,4,103,0,0,2,69,74
                        ,7,0,-66,4,-56,0,21,111,-87,58
                        ,7,0,-66,4,-55,0,-86,29,-79,58
                        ,7,0,-66,4,44,1,6,-42,39,64
                        ,7,0,66,8,100,0,-67,-66,15,74
                        ,7,0,66,8,102,0,74,122,-59,74
                        ,7,0,66,8,101,0,67,-26,-109,73
                        ,7,0,66,8,103,0,92,47,75,74
                        ,7,0,66,8,-56,0,123,22,-100,58
                        ,7,0,66,8,-55,0,85,6,115,58
                        ,7,0,66,8,44,1,57,97,31,64
                        };
            byte[] expected_binary_data1 = new byte[tmp1.Length];
            for(int i=0;i<expected_binary_data1.Length;i++) expected_binary_data1[i] = (byte)tmp1[i];
            byte[] expected_binary_data2 = new byte[tmp2.Length];
            for(int i=0;i<expected_binary_data2.Length;i++) expected_binary_data2[i] = (byte)tmp2[i];
            run_metrics run = new run_metrics();
            c_csharp_comm.read_interop_from_buffer(expected_binary_data1, (uint)expected_binary_data1.Length, run.index_metric_set());
            c_csharp_comm.read_interop_from_buffer(expected_binary_data2, (uint)expected_binary_data2.Length, run.tile_metric_set());

            read_info_vector reads = new read_info_vector();
            reads.Add(new read_info(1, 1, 26));
            reads.Add(new read_info(2, 27, 76));
            run.run_info(new info(
                    "",
                    "",
                    1,
                    new flowcell_layout(2, 2, 2, 16),
                    new string_vector(),
                    new image_dimensions(),
                    reads
            ));
            run.legacy_channel_update(instrument_type.HiSeq);

            bar_plot_data data = new bar_plot_data();
            c_csharp_plot.plot_sample_qc(run, 1, data);
            Assert.AreEqual(data.size(), 1);

		}