protected override void DoTest()
{
OpenDocument("BrukerDIATest.sky");
#region Repicate
//just test the mass error graph with some normal data
SelectNode(SrmDocument.Level.Molecules, 0);
RunUI(SkylineWindow.ShowMassErrorReplicateComparison);
WaitForGraphs();
RunDlg <MassErrorChartPropertyDlg>(SkylineWindow.ShowMassErrorPropertyDlg, dlg => dlg.OkDialog());
RunUI(() =>
{
var pane = GetReplicateGraphPane();
Assert.AreEqual(3, pane.GetTransitionCount());
Assert.AreEqual(9, pane.GetTotalBars());
Assert.AreEqual(3.6, pane.GetMin(), 0.1);
Assert.AreEqual(6.8, pane.GetMax(), 0.1);
});
//test the mass error graph with negative data
SelectNode(SrmDocument.Level.Molecules, 10);
WaitForGraphs();
RunUI(() =>
{
var pane = GetReplicateGraphPane();
Assert.AreEqual(9, pane.GetTransitionCount());
Assert.AreEqual(27, pane.GetTotalBars());
Assert.AreEqual(-22, pane.GetMin(), 0.1);
Assert.AreEqual(23.3, pane.GetMax(), 0.1);
});
//switch to precursors and make sure graph changes
RunUI(() => SkylineWindow.SetDisplayTypeChrom(DisplayTypeChrom.precursors));
WaitForGraphs();
RunUI(() =>
{
var pane = GetReplicateGraphPane();
Assert.AreEqual(3, pane.GetTransitionCount());
});
//switch to products and make sure graph changes
RunUI(() => SkylineWindow.SetDisplayTypeChrom(DisplayTypeChrom.products));
WaitForGraphs();
RunUI(() =>
{
var pane = GetReplicateGraphPane();
Assert.AreEqual(6, pane.GetTransitionCount());
});
//select a protein to test unavailible mass errors
SelectNode(SrmDocument.Level.MoleculeGroups, 0);
WaitForGraphs();
RunUI(() =>
{
var pane = GetReplicateGraphPane();
Assert.AreEqual(0, pane.GetTransitionCount());
Assert.AreEqual(pane.Title.Text, Resources.MassErrorReplicateGraphPane_UpdateGraph_Select_a_peptide_to_see_the_mass_error_graph);
});
//select a peptide with 2 charge states
SelectNode(SrmDocument.Level.Molecules, 44);
WaitForGraphs();
RunUI(() =>
{
var pane = GetReplicateGraphPane();
Assert.AreEqual(2, pane.GetTransitionCount());
Assert.AreEqual(6, pane.GetTotalBars());
});
//select an ion
SelectNode(SrmDocument.Level.Transitions, 0);
WaitForGraphs();
RunUI(() =>
{
var pane = GetReplicateGraphPane();
Assert.AreEqual(3, pane.GetTransitionCount());
Assert.AreEqual(9, pane.GetTotalBars());
});
// test switching to singel transition
RunUI(() => SkylineWindow.SetDisplayTypeChrom(DisplayTypeChrom.single));
WaitForGraphs();
RunUI(() =>
{
var pane = GetReplicateGraphPane();
Assert.AreEqual(1, pane.GetTransitionCount());
});
// test diabeling the legend
RunUI(() => SkylineWindow.ShowMassErrorLegend(false));
WaitForGraphs();
RunUI(() =>
{
var pane = GetReplicateGraphPane();
Assert.IsFalse(pane.Legend.IsVisible);
});
#endregion
#region Peptide
//switch to peptide comparison
RunUI(SkylineWindow.ShowMassErrorPeptideGraph);
WaitForGraphs();
RunUI(() =>
{
var pane = GetPeptideGraphPane();
Assert.AreEqual(1575, pane.GetTotalBars());
Assert.AreEqual(21.86, pane.GetMax(), 0.1);
Assert.AreEqual(-21.17, pane.GetMin(), 0.1);
});
//change scope to protein
RunUI(() => SkylineWindow.AreaScopeTo(AreaScope.protein));
WaitForGraphs();
RunUI(() =>
{
var pane = GetPeptideGraphPane();
Assert.AreEqual(90, pane.GetTotalBars());
});
// test switching to singel transition
RunUI(() => SkylineWindow.SetDisplayTypeChrom(DisplayTypeChrom.total));
WaitForGraphs();
RunUI(() =>
{
var pane = GetPeptideGraphPane();
Assert.AreEqual(1, pane.GetTransitionCount());
});
// test order by mass error
RunUI(() => SkylineWindow.ShowPeptideOrder(SummaryPeptideOrder.mass_error));
WaitForGraphs();
RunUI(() =>
{
var pane = GetPeptideGraphPane();
Assert.AreEqual(pane.GetMin(), pane.CurveList[0][0].Y);
Assert.AreEqual(pane.GetMax(), pane.CurveList[0][9].Y);
});
// test switching replicate
RunUI(() => SkylineWindow.ShowSingleReplicate());
WaitForGraphs();
RunUI(() =>
{
var pane = GetPeptideGraphPane();
Assert.AreEqual(pane.GetMin(), 3.7, 0.1);
Assert.AreEqual(pane.GetMax(), 14.5, 0.1);
});
#endregion
#region Histogram
//switch to histogram
RunUI(SkylineWindow.ShowMassErrorHistogramGraph);
WaitForGraphs();
RunUI(() =>
{
var pane = GetHistogramGraphPane();
Assert.AreEqual(46, pane.GetTotalBars());
Assert.AreEqual(7, pane.GetMax());
Assert.AreEqual(1, pane.GetMin());
});
//switch from singel to all
RunUI(() => SkylineWindow.ShowAverageReplicates());
WaitForGraphs();
RunUI(() =>
{
var pane = GetHistogramGraphPane();
Assert.AreEqual(66, pane.GetTotalBars());
Assert.AreEqual(pane.GetMin(), 1);
Assert.AreEqual(pane.GetMax(), 15);
});
//switch from targets to decoys
RunUI(() => SkylineWindow.ShowPointsTypeMassError(PointsTypeMassError.decoys));
WaitForGraphs();
RunUI(() =>
{
var pane = GetHistogramGraphPane();
Assert.AreEqual(83, pane.GetTotalBars());
Assert.AreEqual(pane.GetMin(), 1);
Assert.AreEqual(pane.GetMax(), 10);
});
//alter the bin size from 0.5 to 2.0
RunUI(() => SkylineWindow.UpdateBinSize(2));
WaitForGraphs();
RunUI(() =>
{
var pane = GetHistogramGraphPane();
Assert.AreEqual(24, pane.GetTotalBars());
Assert.AreEqual(pane.GetMin(), 1);
Assert.AreEqual(pane.GetMax(), 23);
});
// switch display type
RunUI(() => SkylineWindow.ChangeMassErrorDisplayType(DisplayTypeMassError.precursors));
WaitForGraphs();
RunUI(() =>
{
var pane = GetHistogramGraphPane();
Assert.AreEqual(23, pane.GetTotalBars());
Assert.AreEqual(pane.GetMin(), 2);
Assert.AreEqual(pane.GetMax(), 20);
});
// switch transition
RunUI(() => SkylineWindow.ChangeMassErrorTransition(TransitionMassError.all));
WaitForGraphs();
RunUI(() =>
{
var pane = GetHistogramGraphPane();
Assert.AreEqual(26, pane.GetTotalBars());
Assert.AreEqual(pane.GetMin(), 1);
Assert.AreEqual(pane.GetMax(), 55);
});
#endregion
#region Histogram 2D
//switch to histogram 2D
RunUI(SkylineWindow.ShowMassErrorHistogramGraph2D);
WaitForGraphs();
RunUI(() =>
{
var pane = GetHistogram2DGraphPane();
Assert.AreEqual(458, pane.GetPoints());
Assert.AreEqual(24.8, pane.GetMax(), 0.1);
Assert.AreEqual(-23.2, pane.GetMin(), 0.1);
});
//alter the bin size from 2.0 to 0.5
RunUI(() => SkylineWindow.UpdateBinSize(0.5));
WaitForGraphs();
RunUI(() =>
{
var pane = GetHistogram2DGraphPane();
Assert.AreEqual(570, pane.GetPoints());
});
//switch from singel to all
RunUI(() => SkylineWindow.ShowSingleReplicate());
WaitForGraphs();
RunUI(() =>
{
var pane = GetHistogram2DGraphPane();
Assert.AreEqual(212, pane.GetPoints());
});
//switch from targets to decoys
RunUI(() => SkylineWindow.ShowPointsTypeMassError(PointsTypeMassError.targets_1FDR));
WaitForGraphs();
RunUI(() =>
{
var pane = GetHistogram2DGraphPane();
Assert.AreEqual(144, pane.GetPoints());
});
//switch to log scale
RunUI(() => SkylineWindow.SwitchLogScale());
WaitForGraphs();
RunUI(() =>
{
var pane = GetHistogram2DGraphPane();
Assert.AreEqual(144, pane.GetPoints());
});
//change x-axis display
RunUI(() => SkylineWindow.UpdateXAxis(Histogram2DXAxis.mass_to_charge));
WaitForGraphs();
RunUI(() =>
{
var pane = GetHistogram2DGraphPane();
Assert.AreEqual(129, pane.GetPoints());
Assert.AreEqual(12.45, pane.GetMax(), 0.1);
Assert.AreEqual(-0.05, pane.GetMin(), 0.1);
});
#endregion
}