protected void Page_Load(object sender, EventArgs e)
{
string SamplePath = WebConfigurationManager.ConnectionStrings["SampleData"].ConnectionString;
if (Directory.Exists(SamplePath) && ddlSamples.Items.Count == 0)
{
string[] Files = Directory.GetFiles(SamplePath);
foreach (string samplefile in Files)
{
string FileName = samplefile.Substring(samplefile.LastIndexOf("\\") + 1);
this.ddlSamples.Items.Add(FileName);
}
}
if (!Page.IsPostBack)
{
ddlModules.Items.Clear();
try
{
COPaWS.COPaWS ws = new COPaWS.COPaWS();
NetworkCredential objCredential = new NetworkCredential(ConfigurationSettings.AppSettings["COPaWSUser"], ConfigurationSettings.AppSettings["COPaWSPassword"]);
ws.Credentials = objCredential;
string[] Mods = ws.GetLibraryModules();
foreach (string mod in Mods)
{
this.ddlModules.Items.Add(mod);
}
this.ddlModules.Text = this.ddlModules.Items[0].Text;
}
catch (Exception ex)
{
this.lbMessage.Text = "Open the COPa Web Service with error. Please contact with COPa Library!";
}
}
}
protected void Page_Load(object sender, EventArgs e)
{
string queryShow = Request.QueryString["showInfo"];
NoInfo = queryShow;
if (NoInfo == "T")
{ // collapse
informationShow.Text = @"<div id='helpNote' class='collapse'>";
informationBtn.Text = @"<button type='button' class='close button collapsed' data-toggle='collapse' data-target='#helpNote'>×</button>";
}
else
{
informationShow.Text = @"<div id='helpNote' class='collapse in'>";
informationBtn.Text = @"<button type='button' class='close button' data-toggle='collapse' data-target='#helpNote'>×</button>";
}
cbUseDefault.Attributes.Add("onclick", "return cbevent();");
cbUseURL.Attributes.Add("onclick", "return UsingURL();");
cbHighResolution.Attributes.Add("onclick", "return HighResolution();");
string strMsg = Request.QueryString["MSG"];
if (strMsg != "" && strMsg != null)
{
this.lbReportError.Text = "No report availabe for your query!";
}
//task ID was not found in the task_seq table
if (cbUseURL.Checked)
{
this.InputFile1.Style.Remove("display");
this.InputFile1.Style.Add("display", "none");
this.URLPanel.Style.Remove("display");
this.URLPanel.Style.Add("display", "block");
//call url panel and disable file browse panel
}
if (!cbUseDefault.Checked)
{
this.ParameterPanel.Style.Remove("display");
this.ParameterPanel.Style.Add("display", "block");
//call in detailed settings panel
}
//btUpload.Attributes.Add("onclick", "return ShowProgress();");
if (!Page.IsPostBack)
{
this.ddlModels.Items.Clear();
COPaWS.COPaWS ws = new COPaWS.COPaWS();
NetworkCredential objCredential = new NetworkCredential(ConfigurationSettings.AppSettings["COPaWSUser"], ConfigurationSettings.AppSettings["COPaWSPassword"]);
ws.Credentials = objCredential;
string[] Mods = ws.GetLibraryModules();
foreach (string mod in Mods)
{
this.ddlModels.Items.Add(mod);
}
this.ddlModels.Text = this.ddlModels.Items[0].Text;
//initializing dropdown menu
}
}
void UpdateSearchingCondition(string TaskID, float confidence, int distinctPeptides, int UniquePeptides)
{
string scLocation = WebConfigurationManager.ConnectionStrings["UploadPath"].ConnectionString + TaskID + "\\searchcondition.xml";
FileInfo fi = new FileInfo(scLocation);
if (fi.Exists)
{
SearchingParameters mySP = new SearchingParameters(scLocation);
mySP.ConfidenceLevel = confidence;
mySP.DistinctPeptide = distinctPeptides;
mySP.UniquePeptide = UniquePeptides;
mySP.SaveSettings();
}
COPaWS.COPaWS copaws = new COPaWS.COPaWS();
if (copaws.ChangeCreteria(TaskID, this.lbmzML.Text.Replace("<br/>", "\r\n")))
{
Response.Redirect(string.Format("Report.aspx?TaskID={0}", TaskID));
}
}
protected void Page_Load(object sender, EventArgs e)
{
string queryShow = Request.QueryString["showInfo"];
NoInfo = queryShow;
if (NoInfo == "T")
{ // collapse
informationShow.Text = @"<div id='helpNote' class='collapse'>";
informationBtn.Text = @"<button type='button' class='close button collapsed' data-toggle='collapse' data-target='#helpNote'>×</button>";
}
else
{
informationShow.Text = @"<div id='helpNote' class='collapse in'>";
informationBtn.Text = @"<button type='button' class='close button' data-toggle='collapse' data-target='#helpNote'>×</button>";
}
cbUseDefault.Attributes.Add("onclick", "return cbevent();");
cbUseURL.Attributes.Add("onclick", "return UsingURL();");
cbHighResolution.Attributes.Add("onclick", "return HighResolution();");
string strMsg = Request.QueryString["MSG"];
if (strMsg != "" && strMsg != null)
this.lbReportError.Text = "No report availabe for your query!";
//task ID was not found in the task_seq table
if (cbUseURL.Checked)
{
this.InputFile1.Style.Remove("display");
this.InputFile1.Style.Add("display", "none");
this.URLPanel.Style.Remove("display");
this.URLPanel.Style.Add("display", "block");
//call url panel and disable file browse panel
}
if (!cbUseDefault.Checked)
{
this.ParameterPanel.Style.Remove("display");
this.ParameterPanel.Style.Add("display", "block");
//call in detailed settings panel
}
//btUpload.Attributes.Add("onclick", "return ShowProgress();");
if (!Page.IsPostBack)
{
this.ddlModels.Items.Clear();
COPaWS.COPaWS ws = new COPaWS.COPaWS();
NetworkCredential objCredential = new NetworkCredential(ConfigurationSettings.AppSettings["COPaWSUser"], ConfigurationSettings.AppSettings["COPaWSPassword"]);
ws.Credentials = objCredential;
string[] Mods = ws.GetLibraryModules();
foreach (string mod in Mods)
{
this.ddlModels.Items.Add(mod);
}
this.ddlModels.Text = this.ddlModels.Items[0].Text;
//initializing dropdown menu
}
}
private void DoSearchInWS()
{
double[] NormalizedSpectrum = new double[4096];
string[] lines = this.tbDTA.Text.Split(new string[] { "\r\n" }, StringSplitOptions.RemoveEmptyEntries);
if (lines.Length == 0)
{
lbMessage.Text = "Please input spectrum data.";
return;
}
SessionID = DateTime.Now.ToString("yyyy_MM_dd");
IndexID = DateTime.Now.ToString("fffffff");
// string UploadPath = WebConfigurationManager.ConnectionStrings["UploadPath"].ConnectionString + string.Format ("WebDta\\{0}\\",SessionID );
// DirectoryInfo newpath = new DirectoryInfo(UploadPath);
// if (!newpath.Exists)
// {
// newpath.Create();
// }
//string dtaFilename = UploadPath + IndexID + ".dta";
//try
//{
// using (StreamWriter sw = new StreamWriter(dtaFilename, true))
// {
foreach (string line in lines)
{
//sw.WriteLine(line);
if (char.IsDigit(line, 0))
{
String [] sperator = line.Split(new string [] { " ", "\t" }, StringSplitOptions.RemoveEmptyEntries);
string mz = sperator[0];
string intensity = sperator[1];
MZintensitiy mzi = new MZintensitiy();
try
{
mzi.mz = float.Parse(mz);
mzi.intensity = float.Parse(intensity);
if (mzi.mz > NoiseCutoff)
{
double dintensity = (double)mzi.intensity;
NormalizedSpectrum[(int)mzi.mz] += dintensity;
}
//SpectrumData.Add(mzi);
}
catch (Exception ex)
{ }
}
}
// sw.Close();
// }
//}
// catch (Exception ex)
//{
// this.lbMessage.Text = string.Format ("Write dta file to server with error:{0} ",ex.ToString ());
// }
//double TotalNormal = 0;
//foreach (double mz in NormalizedSpectrum)
//{
// TotalNormal += mz * mz;
//}
//TotalNormal = Math.Sqrt(TotalNormal);
//for (int i = 0; i < 4096; i++)
//{
// NormalizedSpectrum[i] = NormalizedSpectrum[i] / TotalNormal;
//}
int slideSize = int.Parse(this.ddlChargeState.Text);
float PrecursorMZ = float.Parse(this.tbPrecursorMZ.Text);
float mzTolerance = float.Parse(this.tbTolerance.Text);
COPaWS.COPaWS webserv = new COPaWS.COPaWS();
NetworkCredential objCredential = new NetworkCredential(ConfigurationSettings.AppSettings["COPaWSUser"], ConfigurationSettings.AppSettings["COPaWSPassword"]);
webserv.Credentials = objCredential;
COPaWS.SearchingCondition sc = new COPaWS.SearchingCondition();
sc.bUseNoiseLibrary = true;
sc.bStatisticSearching = false;
sc.bHighResolution = false;
sc.fPrecursorWindow = mzTolerance;
sc.iSlideSize = slideSize;
COPaWS.SearchingThreadPair tp = new COPaWS.SearchingThreadPair();
tp.DetaDecoyScore = float.Parse(this.tbCutoff.Text);
tp.MatchScore = 0.0F;
sc.NormalSearchCondition = new COPaWS.SearchingThreadPair[] { tp };
COPaWS.Module lib_mod = COPaWS.Module.human_heart_mitochondria;
if (this.ddlModules.Text != null)
{
try
{
lib_mod = (COPaWS.Module)(int.Parse(this.ddlModules.Text.Substring(0, this.ddlModules.Text.IndexOf("."))) - 1);
}
catch (Exception ex)
{
lbMessage.Text = "Could not recognize the library module!";
return;
}
}
COPaWS.Module[] Modules = new COPaWS.Module[] { lib_mod };
COPaWS.MSPepetideInfo[] results = webserv.SearchDTA2("WebDta", SessionID, IndexID, NormalizedSpectrum, PrecursorMZ, Modules, sc, 2);
if (results != null)
{
string PepIDURL = "<a href='PeptideInfo.aspx?QType=Peptide+ID&QValue={0}' Target='_blank'>{1}</a>";
string SpectrumURL = "<a href='MatchSpectrum.aspx?QValue={0}&SessionID={1}&ST=RUN&TaskID={2}&File={3}' Target='_blank'><img src='_image/mirror_spectrum.gif' /></a>";
foreach (COPaWS.MSPepetideInfo mspep in results)
{
TableRow trResult = new TableRow();
TableCell tcPepID = new TableCell();
string strPepID = mspep.COPaPeptideID;
tcPepID.Text = string.Format(PepIDURL, strPepID, strPepID);
tcPepID.HorizontalAlign = HorizontalAlign.Left;
trResult.Cells.Add(tcPepID);
TableCell tcPepSeq = new TableCell();
string PeptideSeq = mspep.PeptideSequence;
//string ModifiedType = mspep.ModifiedType;
tcPepSeq.Text = PeptideSeq;
tcPepSeq.HorizontalAlign = HorizontalAlign.Left;
trResult.Cells.Add(tcPepSeq);
TableCell tcModifiedType = new TableCell();
tcModifiedType.Text = "";// ModifiedType;
tcModifiedType.HorizontalAlign = HorizontalAlign.Left;
trResult.Cells.Add(tcModifiedType);
//TableCell tcDotProduct = new TableCell();
//double dp = double.Parse(foundRows[i].ItemArray[1].ToString());
//tcDotProduct.Text = string.Format("{0:F3}", dp);
//tcDotProduct.HorizontalAlign = HorizontalAlign.Right;
//trResult.Cells.Add(tcDotProduct);
//TableCell tcDotBias = new TableCell();
//double db = double.Parse(foundRows[i].ItemArray[2].ToString());
//tcDotBias.Text = string.Format("{0:F3}", db);
//tcDotBias.HorizontalAlign = HorizontalAlign.Right;
//trResult.Cells.Add(tcDotBias);
TableCell tcFscore = new TableCell();
double finalScore = double.Parse(mspep.DecoyScore);// double.Parse(foundRows[i].ItemArray[3].ToString());
tcFscore.Text = string.Format("{0:F3}", finalScore);
tcFscore.HorizontalAlign = HorizontalAlign.Right;
trResult.Cells.Add(tcFscore);
TableCell tcSpectrum = new TableCell();
tcSpectrum.Text = string.Format(SpectrumURL, mspep.LibSpectraSeq, IndexID, "WebDta", SessionID);
tcSpectrum.HorizontalAlign = HorizontalAlign.Center;
trResult.Cells.Add(tcSpectrum);
tbPeptides.Rows.Add(trResult);
}
if (results.Length > 0)
{
lbMessage.Text = string.Format(" {0} spectra matched!", results.Length);
}
else
{
lbMessage.Text = string.Format("no spectrum was matched under your criteria, you could adjust the cutoff and try again!");
}
}
else
{
lbMessage.Text = string.Format("no spectrum was matched under your criteria, you could adjust the cutoff and try again!");
}
}
private void ResetReport()
{
//string strOldFile = WebConfigurationManager.ConnectionStrings["UploadPath"].ConnectionString + TaskID + "\\Result.txt";
//string strBackFile = WebConfigurationManager.ConnectionStrings["UploadPath"].ConnectionString + TaskID + "\\Result.txt.back";
//string strFreqFile = WebConfigurationManager.ConnectionStrings["UploadPath"].ConnectionString + TaskID + "\\Result.txt.Freq";
float threshold = 0.0f;
try
{
threshold = float.Parse(this.tbThreshold.Text);
if (threshold < 0 || threshold > 100)
{
lbMessage.Text = "Please input a number between 1 and 100.";
return;
}
}
catch (Exception ex)
{
lbMessage.Text = "Please input a number.";
return;
}
COPaWS.COPaWS copaws = new COPaWS.COPaWS();
if (copaws.ChangeThreshold(TaskID, this.lbmzML.Text.Replace("<br/>", "\r\n"), threshold))
{
Response.Redirect(string.Format("Report.aspx?TaskID={0}", TaskID));
}
// string strNewFreqFile = strFreqFile + ".back";
// FileInfo fi = new FileInfo(strFreqFile);
// fi.CopyTo(strNewFreqFile,true );
// StreamWriter freqsw = new StreamWriter(strFreqFile, false);
// StreamReader freqsr = new StreamReader(strNewFreqFile);
// string line = "";
// float newPossiblity = 0.0f;
// try
// {
// while ((line = freqsr.ReadLine()) != null)
// {
// if (line.StartsWith("H"))
// {
// string[] tokens = line.Split(new string[] { "|" }, StringSplitOptions.RemoveEmptyEntries);
// string miu1 = tokens[1];
// string sigma1 = tokens[2];
// string miu2 = tokens[3];
// string sigma2 = tokens[4];
// string strthreshold = threshold.ToString();
// string falseNumber = tokens[6];
// string trueNumber = tokens[7];
// freqsw.WriteLine(string.Format("H|{0}|{1}|{2}|{3}|{4}|{5}|{6}|", miu1, sigma1, miu2, sigma2, strthreshold, falseNumber, trueNumber));
// newPossiblity = getPossiblity(miu1, sigma1, miu2, sigma2, falseNumber, trueNumber, threshold);
// }
// else
// freqsw.WriteLine(line);
// }
// }
// catch (Exception ex)
// {
// DBInterface.LogEvent(ex.ToString(),System.Diagnostics.EventLogEntryType.Error );
// }
// finally
// {
// freqsw.Close();
// freqsr.Close();
// }
// //delte the old file
// StreamWriter sw = new StreamWriter(strOldFile, false);
// StreamReader sr = new StreamReader(strBackFile);
// try
// {
// while ((line = sr.ReadLine()) != null)
// {
// string[] tokens = line.Split(new string[] { "|" }, StringSplitOptions.None);
// if (tokens[0] == "Y")
// {
// string peptideID = tokens[2];
// if ((float.Parse(tokens[10])) * 100 >= threshold)
// {
// sw.WriteLine(line);
// }
// }
// }
// }
// catch (Exception ex)
// {
// DBInterface.LogEvent(ex.ToString(), System.Diagnostics.EventLogEntryType.Error);
// }
// finally
// {
// sr.Close();
// sw.Close();
// }
//string sc = string.Format("Threshold set at {0}, peptide accuracy rate is {1}%.", threshold/100,newPossiblity );
//int NeedDistinctPeptide = UpdateSearchConditionFile(TaskID, newPossiblity);
//CreateReport(TaskID, this.lbmzML.Text.Replace("<br/>", "\r\n"), this.lbSearchingModule.Text, sc,NeedDistinctPeptide );
}
void UpdateSearchingCondition(string TaskID, float confidence, int distinctPeptides, int UniquePeptides)
{
string scLocation = WebConfigurationManager.ConnectionStrings["UploadPath"].ConnectionString + TaskID + "\\searchcondition.xml";
FileInfo fi = new FileInfo(scLocation);
if (fi.Exists)
{
SearchingParameters mySP = new SearchingParameters(scLocation);
mySP.ConfidenceLevel = confidence;
mySP.DistinctPeptide = distinctPeptides;
mySP.UniquePeptide = UniquePeptides;
mySP.SaveSettings();
}
COPaWS.COPaWS copaws = new COPaWS.COPaWS();
if (copaws.ChangeCreteria(TaskID, this.lbmzML.Text.Replace("<br/>", "\r\n")))
Response.Redirect(string.Format("Report.aspx?TaskID={0}", TaskID));
}
private void ResetReport()
{
//string strOldFile = WebConfigurationManager.ConnectionStrings["UploadPath"].ConnectionString + TaskID + "\\Result.txt";
//string strBackFile = WebConfigurationManager.ConnectionStrings["UploadPath"].ConnectionString + TaskID + "\\Result.txt.back";
//string strFreqFile = WebConfigurationManager.ConnectionStrings["UploadPath"].ConnectionString + TaskID + "\\Result.txt.Freq";
float threshold = 0.0f;
try
{
threshold = float.Parse(this.tbThreshold.Text);
if (threshold < 0 || threshold > 100)
{
lbMessage.Text = "Please input a number between 1 and 100.";
return;
}
}
catch (Exception ex)
{
lbMessage.Text = "Please input a number.";
return;
}
COPaWS.COPaWS copaws = new COPaWS.COPaWS();
if (copaws.ChangeThreshold(TaskID, this.lbmzML.Text.Replace("<br/>", "\r\n"), threshold))
Response.Redirect(string.Format("Report.aspx?TaskID={0}", TaskID));
// string strNewFreqFile = strFreqFile + ".back";
// FileInfo fi = new FileInfo(strFreqFile);
// fi.CopyTo(strNewFreqFile,true );
// StreamWriter freqsw = new StreamWriter(strFreqFile, false);
// StreamReader freqsr = new StreamReader(strNewFreqFile);
// string line = "";
// float newPossiblity = 0.0f;
// try
// {
// while ((line = freqsr.ReadLine()) != null)
// {
// if (line.StartsWith("H"))
// {
// string[] tokens = line.Split(new string[] { "|" }, StringSplitOptions.RemoveEmptyEntries);
// string miu1 = tokens[1];
// string sigma1 = tokens[2];
// string miu2 = tokens[3];
// string sigma2 = tokens[4];
// string strthreshold = threshold.ToString();
// string falseNumber = tokens[6];
// string trueNumber = tokens[7];
// freqsw.WriteLine(string.Format("H|{0}|{1}|{2}|{3}|{4}|{5}|{6}|", miu1, sigma1, miu2, sigma2, strthreshold, falseNumber, trueNumber));
// newPossiblity = getPossiblity(miu1, sigma1, miu2, sigma2, falseNumber, trueNumber, threshold);
// }
// else
// freqsw.WriteLine(line);
// }
// }
// catch (Exception ex)
// {
// DBInterface.LogEvent(ex.ToString(),System.Diagnostics.EventLogEntryType.Error );
// }
// finally
// {
// freqsw.Close();
// freqsr.Close();
// }
// //delte the old file
// StreamWriter sw = new StreamWriter(strOldFile, false);
// StreamReader sr = new StreamReader(strBackFile);
// try
// {
// while ((line = sr.ReadLine()) != null)
// {
// string[] tokens = line.Split(new string[] { "|" }, StringSplitOptions.None);
// if (tokens[0] == "Y")
// {
// string peptideID = tokens[2];
// if ((float.Parse(tokens[10])) * 100 >= threshold)
// {
// sw.WriteLine(line);
// }
// }
// }
// }
// catch (Exception ex)
// {
// DBInterface.LogEvent(ex.ToString(), System.Diagnostics.EventLogEntryType.Error);
// }
// finally
// {
// sr.Close();
// sw.Close();
// }
//string sc = string.Format("Threshold set at {0}, peptide accuracy rate is {1}%.", threshold/100,newPossiblity );
//int NeedDistinctPeptide = UpdateSearchConditionFile(TaskID, newPossiblity);
//CreateReport(TaskID, this.lbmzML.Text.Replace("<br/>", "\r\n"), this.lbSearchingModule.Text, sc,NeedDistinctPeptide );
}
private void DoSearchInWS()
{
double[] NormalizedSpectrum = new double[4096];
string[] lines = this.tbDTA.Text.Split(new string[] { "\r\n" }, StringSplitOptions.RemoveEmptyEntries);
if (lines.Length == 0)
{
lbMessage.Text = "Please input spectrum data.";
return;
}
SessionID = DateTime.Now.ToString("yyyy_MM_dd") ;
IndexID = DateTime.Now.ToString("fffffff");
// string UploadPath = WebConfigurationManager.ConnectionStrings["UploadPath"].ConnectionString + string.Format ("WebDta\\{0}\\",SessionID );
// DirectoryInfo newpath = new DirectoryInfo(UploadPath);
// if (!newpath.Exists)
// {
// newpath.Create();
// }
//string dtaFilename = UploadPath + IndexID + ".dta";
//try
//{
// using (StreamWriter sw = new StreamWriter(dtaFilename, true))
// {
foreach (string line in lines)
{
//sw.WriteLine(line);
if (char.IsDigit(line, 0))
{
String [] sperator = line.Split (new string []{" ","\t"},StringSplitOptions.RemoveEmptyEntries );
string mz = sperator[0];
string intensity = sperator[1];
MZintensitiy mzi = new MZintensitiy();
try
{
mzi.mz = float.Parse(mz);
mzi.intensity = float.Parse(intensity);
if (mzi.mz > NoiseCutoff)
{
double dintensity = (double)mzi.intensity;
NormalizedSpectrum[(int)mzi.mz] += dintensity;
}
//SpectrumData.Add(mzi);
}
catch (Exception ex)
{ }
}
}
// sw.Close();
// }
//}
// catch (Exception ex)
//{
// this.lbMessage.Text = string.Format ("Write dta file to server with error:{0} ",ex.ToString ());
// }
//double TotalNormal = 0;
//foreach (double mz in NormalizedSpectrum)
//{
// TotalNormal += mz * mz;
//}
//TotalNormal = Math.Sqrt(TotalNormal);
//for (int i = 0; i < 4096; i++)
//{
// NormalizedSpectrum[i] = NormalizedSpectrum[i] / TotalNormal;
//}
int slideSize = int.Parse(this.ddlChargeState.Text);
float PrecursorMZ = float.Parse(this.tbPrecursorMZ.Text);
float mzTolerance = float.Parse(this.tbTolerance.Text);
COPaWS.COPaWS webserv = new COPaWS.COPaWS();
NetworkCredential objCredential = new NetworkCredential(ConfigurationSettings.AppSettings["COPaWSUser"], ConfigurationSettings.AppSettings["COPaWSPassword"]);
webserv.Credentials = objCredential;
COPaWS.SearchingCondition sc = new COPaWS.SearchingCondition ();
sc.bUseNoiseLibrary = true ;
sc.bStatisticSearching = false ;
sc.bHighResolution = false;
sc.fPrecursorWindow = mzTolerance ;
sc.iSlideSize = slideSize ;
COPaWS.SearchingThreadPair tp = new COPaWS.SearchingThreadPair() ;
tp.DetaDecoyScore = float.Parse (this.tbCutoff.Text );
tp.MatchScore = 0.0F;
sc.NormalSearchCondition = new COPaWS.SearchingThreadPair[] {tp} ;
COPaWS.Module lib_mod = COPaWS.Module.human_heart_mitochondria ;
if (this.ddlModules.Text != null)
{
try
{
lib_mod = (COPaWS.Module)(int.Parse(this.ddlModules.Text.Substring(0, this.ddlModules.Text.IndexOf("."))) - 1);
}
catch (Exception ex)
{
lbMessage.Text = "Could not recognize the library module!";
return;
}
}
COPaWS.Module[] Modules = new COPaWS.Module[] { lib_mod };
COPaWS.MSPepetideInfo[] results = webserv.SearchDTA2("WebDta", SessionID, IndexID, NormalizedSpectrum, PrecursorMZ, Modules, sc, 2);
if (results != null)
{
string PepIDURL = "<a href='PeptideInfo.aspx?QType=Peptide+ID&QValue={0}' Target='_blank'>{1}</a>";
string SpectrumURL = "<a href='MatchSpectrum.aspx?QValue={0}&SessionID={1}&ST=RUN&TaskID={2}&File={3}' Target='_blank'><img src='_image/mirror_spectrum.gif' /></a>";
foreach (COPaWS.MSPepetideInfo mspep in results)
{
TableRow trResult = new TableRow();
TableCell tcPepID = new TableCell();
string strPepID = mspep.COPaPeptideID;
tcPepID.Text = string.Format(PepIDURL, strPepID, strPepID);
tcPepID.HorizontalAlign = HorizontalAlign.Left;
trResult.Cells.Add(tcPepID);
TableCell tcPepSeq = new TableCell();
string PeptideSeq = mspep.PeptideSequence;
//string ModifiedType = mspep.ModifiedType;
tcPepSeq.Text = PeptideSeq;
tcPepSeq.HorizontalAlign = HorizontalAlign.Left;
trResult.Cells.Add(tcPepSeq);
TableCell tcModifiedType = new TableCell();
tcModifiedType.Text = "";// ModifiedType;
tcModifiedType.HorizontalAlign = HorizontalAlign.Left;
trResult.Cells.Add(tcModifiedType);
//TableCell tcDotProduct = new TableCell();
//double dp = double.Parse(foundRows[i].ItemArray[1].ToString());
//tcDotProduct.Text = string.Format("{0:F3}", dp);
//tcDotProduct.HorizontalAlign = HorizontalAlign.Right;
//trResult.Cells.Add(tcDotProduct);
//TableCell tcDotBias = new TableCell();
//double db = double.Parse(foundRows[i].ItemArray[2].ToString());
//tcDotBias.Text = string.Format("{0:F3}", db);
//tcDotBias.HorizontalAlign = HorizontalAlign.Right;
//trResult.Cells.Add(tcDotBias);
TableCell tcFscore = new TableCell();
double finalScore = double.Parse(mspep.DecoyScore);// double.Parse(foundRows[i].ItemArray[3].ToString());
tcFscore.Text = string.Format("{0:F3}", finalScore);
tcFscore.HorizontalAlign = HorizontalAlign.Right;
trResult.Cells.Add(tcFscore);
TableCell tcSpectrum = new TableCell();
tcSpectrum.Text = string.Format(SpectrumURL, mspep.LibSpectraSeq, IndexID, "WebDta", SessionID);
tcSpectrum.HorizontalAlign = HorizontalAlign.Center;
trResult.Cells.Add(tcSpectrum);
tbPeptides.Rows.Add(trResult);
}
if (results.Length > 0)
lbMessage.Text = string.Format(" {0} spectra matched!", results.Length);
else
lbMessage.Text = string.Format("no spectrum was matched under your criteria, you could adjust the cutoff and try again!");
}
else
lbMessage.Text = string.Format("no spectrum was matched under your criteria, you could adjust the cutoff and try again!");
}
protected void Page_Load(object sender, EventArgs e)
{
string SamplePath = WebConfigurationManager.ConnectionStrings["SampleData"].ConnectionString;
if (Directory.Exists(SamplePath) && ddlSamples.Items.Count ==0)
{
string[] Files = Directory.GetFiles(SamplePath);
foreach (string samplefile in Files)
{
string FileName = samplefile.Substring(samplefile.LastIndexOf("\\")+1);
this.ddlSamples.Items.Add(FileName);
}
}
if (!Page.IsPostBack)
{
ddlModules.Items.Clear();
try
{
COPaWS.COPaWS ws = new COPaWS.COPaWS();
NetworkCredential objCredential = new NetworkCredential(ConfigurationSettings.AppSettings["COPaWSUser"], ConfigurationSettings.AppSettings["COPaWSPassword"]);
ws.Credentials = objCredential;
string[] Mods = ws.GetLibraryModules();
foreach (string mod in Mods)
{
this.ddlModules.Items.Add(mod);
}
this.ddlModules.Text = this.ddlModules.Items[0].Text;
}
catch (Exception ex)
{
this.lbMessage.Text = "Open the COPa Web Service with error. Please contact with COPa Library!";
}
}
}
