public static void Main(string[] args)
{
RunProteinSignificanceClassifier proteinBasedSignificance = new RunProteinSignificanceClassifier();
int numberSamples = 0;
// Parse the ExperimentalDesign File to get info of samples and conditions they belong to
Dictionary <string, List <string> > samplefileConditionRelation = proteinBasedSignificance.ExpermientalDesignParser("C:/Users/Anay/Desktop/UW Madison/Smith Lab/Spectra Data/ExperimentalDesign.tsv"
, ref numberSamples);
// get all conditions and pair them up for Significance classification
List <string> allConditions = new List <string>(samplefileConditionRelation.Keys);
List <List <string> > allTwoConditionCombinations = proteinBasedSignificance.GenerateAllCombinationsOfTwoConditions(allConditions);
foreach (List <string> conditionPair in allTwoConditionCombinations)
{
string firstCondition = conditionPair[0];
string secondCondition = conditionPair[1];
double sOValue = 0.1;
double meanFraction = 0.1;
int maxSignificantCount = 0;
while (meanFraction < 1)
{
while (sOValue < 1)
{
for (int k = 0; k < numberSamples; k++)
{
proteinBasedSignificance = new RunProteinSignificanceClassifier();
//Declaring variables which will be generated after parsing QuantifiedPeptides file
List <ProteinRowInfo> allProteinInfo = new List <ProteinRowInfo>();
List <string> samplesFileNames = new List <string>();
int maxInvalidIntensityValues = k;
proteinBasedSignificance.ProteinDataParser(allProteinInfo, maxInvalidIntensityValues,
samplesFileNames, "C:/Users/Anay/Desktop/UW Madison/Smith Lab/Spectra Data/FlashLFQ_2020-04-26-17-39-35/QuantifiedProteins.tsv");
// imputes missing intensity values for each protein
ImputationProcess imputationProcess = new ImputationProcess();
imputationProcess.RunImputationProcess(allProteinInfo, samplesFileNames, meanFraction);
// Declaring variables which will be generated after T-Tests and Permutation Tests
List <double> observedNValues = new List <double>(); // will store observed N values
List <double> permutedNValues = new List <double>(); // will store permuted N values
StatisticalTests statisticalTests = new StatisticalTests();
// contains proteins and their observed N value, P Value and Log Fold Change
Dictionary <string, List <double> > allProteinObservedStatistics = new Dictionary <string, List <double> >();
// Creating threads for Parallelizing code
ThreadPool.GetMaxThreads(out int workerThreadsCount, out int ioThreadsCount);
int[] threads = Enumerable.Range(0, workerThreadsCount).ToArray();
Parallel.ForEach(threads, (i) =>
{
// Compute observed and permuted N Values for each protein using T Tests and Permutation Testing
for (; i < allProteinInfo.Count; i += workerThreadsCount)
{
ProteinRowInfo proteinRowInfo = allProteinInfo[i];
Dictionary <string, double> samplesintensityData = proteinRowInfo.SamplesIntensityData;
List <string> firstConditionAssociatedSamples = samplefileConditionRelation.GetValueOrDefault(firstCondition);
List <string> secondConditionAssociatedSamples = samplefileConditionRelation.GetValueOrDefault(secondCondition);
List <double> proteinFirstConditionIntensityValues = new List <double>();
List <double> proteinSecondConditionIntensityValues = new List <double>();
// get the protein's intensity values corresponding to the chosen pair of conditions
foreach (string sampleFileName in samplesFileNames)
{
if (firstConditionAssociatedSamples.Contains(sampleFileName))
{
proteinFirstConditionIntensityValues.Add(samplesintensityData[sampleFileName]);
}
if (secondConditionAssociatedSamples.Contains(sampleFileName))
{
proteinSecondConditionIntensityValues.Add(samplesintensityData[sampleFileName]);
}
}
// Compute observed N Values with the chosen pair of conditions using T-Tests and
// store in observedNValues array
List <double> proteinStatistics = statisticalTests.GetNValueUsingTTest(proteinFirstConditionIntensityValues, proteinSecondConditionIntensityValues,
sOValue, false);
// Compute permuted N Values with the chosen pair of conditions using T-Tests and
// store in permutedNValues array
List <double> proteinPermutedNavlues = statisticalTests.GetNValueUsingPermutationtests(proteinFirstConditionIntensityValues,
proteinSecondConditionIntensityValues, sOValue);
// add computed original and permuted statistics for the protein
lock (allProteinObservedStatistics)
{
// add protein and its observed N value, P Value and Log Fold Change in that order
allProteinObservedStatistics.Add(proteinRowInfo.ProteinID, new List <double>()
{
proteinStatistics[0],
proteinStatistics[1], proteinStatistics[2]
});
observedNValues.Add(proteinStatistics[0]);
foreach (double permutedNValue in proteinPermutedNavlues)
{
permutedNValues.Add(permutedNValue);
}
}
}
});
// makes the permuted N values list and the observed N Values list of the same size
proteinBasedSignificance.ResizePermutedArray(permutedNValues, permutedNValues.Count() - observedNValues.Count());
// get the threshold at which we will filter out the significant proteins
double nValueThreshold = statisticalTests.calculateNvaluethreshold(observedNValues, permutedNValues, 0.05);
// determine number of signifcant proteins detected
int newSignificantCount = observedNValues.Count(x => x >= nValueThreshold);
if (newSignificantCount > maxSignificantCount)
{
maxSignificantCount = newSignificantCount;
proteinBasedSignificance.PrintSignificantProtein(allProteinInfo, nValueThreshold, allProteinObservedStatistics,
"C:/Users/Anay/Desktop/UW Madison/Smith Lab/Project 1/ConsoleApp1/ProteinBasedSignificanceModified.csv");
Console.WriteLine("Sig Count - " + maxSignificantCount + "meanFraction - " + meanFraction + "sOValue - "
+ sOValue + "k - " + k);
}
}
sOValue = sOValue + 0.1;
}
sOValue = 0.1;
meanFraction = meanFraction + 0.3;
}
}
}
public static void Main(string[] args)
{
Program proteinBasedSignificance = new Program();
// Parse the ExperimentalDesign File to get info of samples and conditions they belong to
Dictionary <string, List <string> > samplefileConditionRelation = proteinBasedSignificance.ExpermientalDesignParser("C:/Users/Anay/Desktop/UW Madison/Smith Lab/Spectra Data/ExperimentalDesign.tsv");
// get all conditions and pair them up for Significance classification
List <string> allConditions = new List <string>(samplefileConditionRelation.Keys);
List <List <string> > allTwoConditionCombinations = proteinBasedSignificance.GenerateAllCombinationsOfTwoConditions(allConditions);
foreach (List <string> conditionPair in allTwoConditionCombinations)
{
string firstCondition = conditionPair[0];
string secondCondition = conditionPair[1];
double sOValue = 0.1;
double meanFraction = 0.1;
int maxSignificantCount = 0;
while (meanFraction < 1)
{
while (sOValue < 1)
{
for (int k = 1; k < 9; k++)
{
proteinBasedSignificance = new Program();
//Declaring variables which will be generated after parsing QuantifiedPeptides file
List <ProteinRowInfo> allProteinInfo = new List <ProteinRowInfo>();
List <string> samplesFileNames = new List <string>();
int maxInvalidIntensityValues = k;
proteinBasedSignificance.ProteinDataParser(allProteinInfo, maxInvalidIntensityValues,
samplesFileNames, "C:/Users/Anay/Desktop/UW Madison/Smith Lab/Spectra Data/FlashLFQ_2020-04-26-17-39-35/QuantifiedProteins.tsv");
// imputes missing intensity values for each protein
ImputationProcess imputationProcess = new ImputationProcess();
imputationProcess.RunImputationProcess(allProteinInfo, samplesFileNames, meanFraction);
// Declaring variables which will be generated after T-Tests and Permutation Tests
List <double> actualNValues = new List <double>(); // will store actual(real) N values
List <double> actualPValues = new List <double>(); // will store actual(real) P values
List <double> actualLogFoldChange = new List <double>(); // will store actual(real) Log Fold Change values
List <double> permutedNValues = new List <double>(); // will store permuted(fake) N values
StatisticalTests statisticalTests = new StatisticalTests();
// Compute actual and permuted N Values for each protein using T Tests and Permutation Testing
for (int i = 0; i < allProteinInfo.Count; i++)
{
ProteinRowInfo proteinRowInfo = allProteinInfo[i];
Dictionary <string, double> samplesintensityData = proteinRowInfo.SamplesIntensityData;
List <string> firstConditionAssociatedSamples = samplefileConditionRelation.GetValueOrDefault(firstCondition);
List <string> secondConditionAssociatedSamples = samplefileConditionRelation.GetValueOrDefault(secondCondition);
List <double> proteinFirstConditionIntensityValues = new List <double>();
List <double> proteinSecondConditionIntensityValues = new List <double>();
// get the protein's intensity values corresponding to the chosen pair of conditions
foreach (string sampleFileName in samplesFileNames)
{
if (firstConditionAssociatedSamples.Contains(sampleFileName))
{
proteinFirstConditionIntensityValues.Add(samplesintensityData[sampleFileName]);
}
if (secondConditionAssociatedSamples.Contains(sampleFileName))
{
proteinSecondConditionIntensityValues.Add(samplesintensityData[sampleFileName]);
}
}
// Compute actual(real) N Values with the chosen pair of conditions using T-Tests and
// store in actualNValues array
statisticalTests.GetNValueUsingTTest(proteinFirstConditionIntensityValues, proteinSecondConditionIntensityValues,
actualNValues, actualPValues, actualLogFoldChange, sOValue);
// Compute permuted(fake) N Values with the chosen pair of conditions using T-Tests and
// store in permutedNValues array
statisticalTests.GetNValueUsingPermutationtests(proteinFirstConditionIntensityValues, proteinSecondConditionIntensityValues,
permutedNValues, sOValue);
}
// makes the permuted N values list and the actual N Values list of the same size
proteinBasedSignificance.ResizePermutedArray(permutedNValues, permutedNValues.Count() - actualNValues.Count());
// Copy of the actual N values which will be used when determind the N Value threshold for target FDR
List <double> actualNValuesCopy = new List <double>();
for (int i = 0; i < actualNValues.Count; i++)
{
actualNValuesCopy.Add(actualNValues[i]);
}
// get the threshold at which we will filter out the significant proteins
double nValueThreshold = statisticalTests.calculateNvaluethreshold(actualNValuesCopy, permutedNValues, 0.05);
// determine number of signifcant proteins detected
int newSignificantCount = actualNValues.Count(x => x >= nValueThreshold);
if (newSignificantCount > maxSignificantCount)
{
maxSignificantCount = newSignificantCount;
proteinBasedSignificance.PrintSignificantProtein(allProteinInfo, actualNValues, nValueThreshold, actualPValues,
actualLogFoldChange, "C:/Users/Anay/Desktop/UW Madison/Smith Lab/Project 1/ConsoleApp1/ProteinBaseedSignificance.csv");
}
}
sOValue = sOValue + 0.1;
}
sOValue = 0.1;
meanFraction = meanFraction + 0.3;
}
}
}
public void TestImputeData()
{
ProteinRowInfo expectedSampleprotein = new ProteinRowInfo();
expectedSampleprotein.ProteinID = "A0A087WTI9";
expectedSampleprotein.SamplesIntensityData["Intensity_B02_06_161103_A1_HCD_OT_4ul"] = 24.607;
expectedSampleprotein.SamplesIntensityData["Intensity_B02_07_161103_A2_HCD_OT_4ul"] = 23.763;
expectedSampleprotein.SamplesIntensityData["Intensity_B02_16_161103_A3_HCD_OT_4ul"] = 20.783;
expectedSampleprotein.SamplesIntensityData["Intensity_B02_17_161103_A4_HCD_OT_4ul"] = 20.165;
expectedSampleprotein.SamplesIntensityData["Intensity_B02_24_161103_C1_HCD_OT_4ul"] = 20.639;
expectedSampleprotein.SamplesIntensityData["Intensity_B02_09_161103_C2_HCD_OT_4ul"] = 17.223;
expectedSampleprotein.SamplesIntensityData["Intensity_B02_14_161103_C3_HCD_OT_4ul"] = 21.564;
expectedSampleprotein.SamplesIntensityData["Intensity_B02_19_161103_C4_HCD_OT_4ul"] = 20.469;
List <string> sampleFileNames = new List <string>();
sampleFileNames.Add("Intensity_B02_06_161103_A1_HCD_OT_4ul");
sampleFileNames.Add("Intensity_B02_07_161103_A2_HCD_OT_4ul");
sampleFileNames.Add("Intensity_B02_16_161103_A3_HCD_OT_4ul");
sampleFileNames.Add("Intensity_B02_17_161103_A4_HCD_OT_4ul");
sampleFileNames.Add("Intensity_B02_24_161103_C1_HCD_OT_4ul");
sampleFileNames.Add("Intensity_B02_09_161103_C2_HCD_OT_4ul");
sampleFileNames.Add("Intensity_B02_14_161103_C3_HCD_OT_4ul");
sampleFileNames.Add("Intensity_B02_19_161103_C4_HCD_OT_4ul");
double[] sampleMissingCount = new double[8];
sampleMissingCount[0] = 20;
sampleMissingCount[1] = 7;
sampleMissingCount[2] = 11;
sampleMissingCount[3] = 0;
sampleMissingCount[4] = 32;
sampleMissingCount[5] = 2;
sampleMissingCount[6] = 0;
sampleMissingCount[7] = 5;
int[] numberOfValidIntensityValuesInSample = new int[8];
numberOfValidIntensityValuesInSample[0] = 120;
numberOfValidIntensityValuesInSample[1] = 135;
numberOfValidIntensityValuesInSample[2] = 100;
numberOfValidIntensityValuesInSample[3] = 87;
numberOfValidIntensityValuesInSample[4] = 189;
numberOfValidIntensityValuesInSample[5] = 50;
numberOfValidIntensityValuesInSample[6] = 79;
numberOfValidIntensityValuesInSample[7] = 30;
double[] samplesMeanIntensityValue = new double[8];
samplesMeanIntensityValue[0] = 24.4;
samplesMeanIntensityValue[1] = 23.5;
samplesMeanIntensityValue[2] = 22;
samplesMeanIntensityValue[3] = 19.7;
samplesMeanIntensityValue[4] = 21.75;
samplesMeanIntensityValue[5] = 18.2;
samplesMeanIntensityValue[6] = 23.21;
samplesMeanIntensityValue[7] = 21.09;
double[] samplesStandardDeviation = new double[8];
samplesStandardDeviation[0] = 0.2;
samplesStandardDeviation[1] = 1.1;
samplesStandardDeviation[2] = 0.02;
samplesStandardDeviation[3] = 0.7;
samplesStandardDeviation[4] = 1.7;
samplesStandardDeviation[5] = 0.8;
samplesStandardDeviation[6] = 2.4;
samplesStandardDeviation[7] = 0.15;
ProteinRowInfo outputSampleprotein = new ProteinRowInfo();
outputSampleprotein.ProteinID = "A0A087WTI9";
outputSampleprotein.SamplesIntensityData["Intensity_B02_06_161103_A1_HCD_OT_4ul"] = 0;
outputSampleprotein.SamplesIntensityData["Intensity_B02_07_161103_A2_HCD_OT_4ul"] = 23.763;
outputSampleprotein.SamplesIntensityData["Intensity_B02_16_161103_A3_HCD_OT_4ul"] = 20.783;
outputSampleprotein.SamplesIntensityData["Intensity_B02_17_161103_A4_HCD_OT_4ul"] = 20.165;
outputSampleprotein.SamplesIntensityData["Intensity_B02_24_161103_C1_HCD_OT_4ul"] = 0;
outputSampleprotein.SamplesIntensityData["Intensity_B02_09_161103_C2_HCD_OT_4ul"] = 0;
outputSampleprotein.SamplesIntensityData["Intensity_B02_14_161103_C3_HCD_OT_4ul"] = 21.564;
outputSampleprotein.SamplesIntensityData["Intensity_B02_19_161103_C4_HCD_OT_4ul"] = 20.469;
double meanFraction = 0.2;
ImputationProcess imputationProcess = new ImputationProcess();
imputationProcess.ImputeData(outputSampleprotein, samplesMeanIntensityValue, samplesStandardDeviation,
sampleFileNames, sampleMissingCount, numberOfValidIntensityValuesInSample, meanFraction, true);
Assert.That(expectedSampleprotein.SamplesIntensityData,
Is.EqualTo(outputSampleprotein.SamplesIntensityData).Within(0.001));
}
public void TestCalculateNumberOfIntensityValuesOfSample()
{
int[] expectedSampleIntensityValuesCount = new int[8];
expectedSampleIntensityValuesCount[0] = 0;
expectedSampleIntensityValuesCount[1] = 1;
expectedSampleIntensityValuesCount[2] = 1;
expectedSampleIntensityValuesCount[3] = 1;
expectedSampleIntensityValuesCount[4] = 0;
expectedSampleIntensityValuesCount[5] = 1;
expectedSampleIntensityValuesCount[6] = 1;
expectedSampleIntensityValuesCount[7] = 0;
List <string> sampleFileNames = new List <string>();
sampleFileNames.Add("Intensity_B02_06_161103_A1_HCD_OT_4ul");
sampleFileNames.Add("Intensity_B02_07_161103_A2_HCD_OT_4ul");
sampleFileNames.Add("Intensity_B02_16_161103_A3_HCD_OT_4ul");
sampleFileNames.Add("Intensity_B02_17_161103_A4_HCD_OT_4ul");
sampleFileNames.Add("Intensity_B02_24_161103_C1_HCD_OT_4ul");
sampleFileNames.Add("Intensity_B02_09_161103_C2_HCD_OT_4ul");
sampleFileNames.Add("Intensity_B02_14_161103_C3_HCD_OT_4ul");
sampleFileNames.Add("Intensity_B02_19_161103_C4_HCD_OT_4ul");
ProteinRowInfo sampleprotein1 = new ProteinRowInfo();
sampleprotein1.ProteinID = "A0A023KES1";
sampleprotein1.SamplesIntensityData["Intensity_B02_06_161103_A1_HCD_OT_4ul"] = 0;
sampleprotein1.SamplesIntensityData["Intensity_B02_07_161103_A2_HCD_OT_4ul"] = 22.729;
sampleprotein1.SamplesIntensityData["Intensity_B02_16_161103_A3_HCD_OT_4ul"] = 22.347;
sampleprotein1.SamplesIntensityData["Intensity_B02_17_161103_A4_HCD_OT_4ul"] = 22.397;
sampleprotein1.SamplesIntensityData["Intensity_B02_24_161103_C1_HCD_OT_4ul"] = 0;
sampleprotein1.SamplesIntensityData["Intensity_B02_09_161103_C2_HCD_OT_4ul"] = 23.180;
sampleprotein1.SamplesIntensityData["Intensity_B02_14_161103_C3_HCD_OT_4ul"] = 23.293;
sampleprotein1.SamplesIntensityData["Intensity_B02_19_161103_C4_HCD_OT_4ul"] = 0;
ProteinRowInfo sampleprotein2 = new ProteinRowInfo();
sampleprotein2.ProteinID = "A0A024R4E5";
sampleprotein2.SamplesIntensityData["Intensity_B02_06_161103_A1_HCD_OT_4ul"] = 0;
sampleprotein2.SamplesIntensityData["Intensity_B02_07_161103_A2_HCD_OT_4ul"] = 25.535;
sampleprotein2.SamplesIntensityData["Intensity_B02_16_161103_A3_HCD_OT_4ul"] = 0;
sampleprotein2.SamplesIntensityData["Intensity_B02_17_161103_A4_HCD_OT_4ul"] = 0;
sampleprotein2.SamplesIntensityData["Intensity_B02_24_161103_C1_HCD_OT_4ul"] = 25.370;
sampleprotein2.SamplesIntensityData["Intensity_B02_09_161103_C2_HCD_OT_4ul"] = 24;
sampleprotein2.SamplesIntensityData["Intensity_B02_14_161103_C3_HCD_OT_4ul"] = 25.359;
sampleprotein2.SamplesIntensityData["Intensity_B02_19_161103_C4_HCD_OT_4ul"] = 0;
int[] outputSampleIntensityValuesCount = new int[8];
double[] outputSampleAllIntensityValuesSum = new double[8];
ImputationProcess imputationProcess = new ImputationProcess();
imputationProcess.CalculateNumberAndSumOfIntensityValuesOfSample(sampleprotein1,
outputSampleAllIntensityValuesSum, sampleFileNames, outputSampleIntensityValuesCount);
CollectionAssert.AreEqual(expectedSampleIntensityValuesCount, outputSampleIntensityValuesCount);
expectedSampleIntensityValuesCount[0] = 0;
expectedSampleIntensityValuesCount[1] = 2;
expectedSampleIntensityValuesCount[2] = 1;
expectedSampleIntensityValuesCount[3] = 1;
expectedSampleIntensityValuesCount[4] = 1;
expectedSampleIntensityValuesCount[5] = 2;
expectedSampleIntensityValuesCount[6] = 2;
expectedSampleIntensityValuesCount[7] = 0;
imputationProcess.CalculateNumberAndSumOfIntensityValuesOfSample(sampleprotein2,
outputSampleAllIntensityValuesSum, sampleFileNames, outputSampleIntensityValuesCount);
CollectionAssert.AreEqual(expectedSampleIntensityValuesCount, outputSampleIntensityValuesCount);
}
public void TestCalculateSampleStandardDeviationNumerator()
{
List <string> sampleFileNames = new List <string>();
sampleFileNames.Add("Intensity_B02_06_161103_A1_HCD_OT_4ul");
sampleFileNames.Add("Intensity_B02_07_161103_A2_HCD_OT_4ul");
sampleFileNames.Add("Intensity_B02_16_161103_A3_HCD_OT_4ul");
sampleFileNames.Add("Intensity_B02_17_161103_A4_HCD_OT_4ul");
sampleFileNames.Add("Intensity_B02_24_161103_C1_HCD_OT_4ul");
sampleFileNames.Add("Intensity_B02_09_161103_C2_HCD_OT_4ul");
sampleFileNames.Add("Intensity_B02_14_161103_C3_HCD_OT_4ul");
sampleFileNames.Add("Intensity_B02_19_161103_C4_HCD_OT_4ul");
ProteinRowInfo sampleprotein1 = new ProteinRowInfo();
sampleprotein1.ProteinID = "A0A023KES1";
sampleprotein1.SamplesIntensityData["Intensity_B02_06_161103_A1_HCD_OT_4ul"] = 0;
sampleprotein1.SamplesIntensityData["Intensity_B02_07_161103_A2_HCD_OT_4ul"] = 22.729;
sampleprotein1.SamplesIntensityData["Intensity_B02_16_161103_A3_HCD_OT_4ul"] = 22.347;
sampleprotein1.SamplesIntensityData["Intensity_B02_17_161103_A4_HCD_OT_4ul"] = 22.397;
sampleprotein1.SamplesIntensityData["Intensity_B02_24_161103_C1_HCD_OT_4ul"] = 0;
sampleprotein1.SamplesIntensityData["Intensity_B02_09_161103_C2_HCD_OT_4ul"] = 23.180;
sampleprotein1.SamplesIntensityData["Intensity_B02_14_161103_C3_HCD_OT_4ul"] = 23.293;
sampleprotein1.SamplesIntensityData["Intensity_B02_19_161103_C4_HCD_OT_4ul"] = 0;
ProteinRowInfo sampleprotein2 = new ProteinRowInfo();
sampleprotein2.ProteinID = "A0A024R4E5";
sampleprotein2.SamplesIntensityData["Intensity_B02_06_161103_A1_HCD_OT_4ul"] = 0;
sampleprotein2.SamplesIntensityData["Intensity_B02_07_161103_A2_HCD_OT_4ul"] = 25.535;
sampleprotein2.SamplesIntensityData["Intensity_B02_16_161103_A3_HCD_OT_4ul"] = 0;
sampleprotein2.SamplesIntensityData["Intensity_B02_17_161103_A4_HCD_OT_4ul"] = 0;
sampleprotein2.SamplesIntensityData["Intensity_B02_24_161103_C1_HCD_OT_4ul"] = 25.370;
sampleprotein2.SamplesIntensityData["Intensity_B02_09_161103_C2_HCD_OT_4ul"] = 24;
sampleprotein2.SamplesIntensityData["Intensity_B02_14_161103_C3_HCD_OT_4ul"] = 25.359;
sampleprotein2.SamplesIntensityData["Intensity_B02_19_161103_C4_HCD_OT_4ul"] = 0;
double[] samplesMeanIntensityValue = new double[8];
samplesMeanIntensityValue[0] = 0;
samplesMeanIntensityValue[1] = (22.729 + 25.535) / 2;
samplesMeanIntensityValue[2] = 22.347 / 2;
samplesMeanIntensityValue[3] = 22.397 / 2;
samplesMeanIntensityValue[4] = 25.370 / 2;
samplesMeanIntensityValue[5] = (23.180 + 24) / 2;
samplesMeanIntensityValue[6] = (23.293 + 25.359) / 2;
samplesMeanIntensityValue[7] = 0;
double[] expectedSamplesStandardDeviationNumerators = new double[8];
expectedSamplesStandardDeviationNumerators[0] = 0;
expectedSamplesStandardDeviationNumerators[1] = Math.Pow(22.729 - samplesMeanIntensityValue[1], 2);
expectedSamplesStandardDeviationNumerators[2] = Math.Pow(22.347 - samplesMeanIntensityValue[2], 2);
expectedSamplesStandardDeviationNumerators[3] = Math.Pow(22.397 - samplesMeanIntensityValue[3], 2);
expectedSamplesStandardDeviationNumerators[4] = 0;
expectedSamplesStandardDeviationNumerators[5] = Math.Pow(23.180 - samplesMeanIntensityValue[5], 2);
expectedSamplesStandardDeviationNumerators[6] = Math.Pow(23.293 - samplesMeanIntensityValue[6], 2);
expectedSamplesStandardDeviationNumerators[7] = 0;
double[] outputSamplesStandardDeviationNumerators = new double[8];
ImputationProcess imputationProcess = new ImputationProcess();
imputationProcess.CalculateSampleStandardDeviationNumerator(sampleprotein1, outputSamplesStandardDeviationNumerators,
sampleFileNames, samplesMeanIntensityValue);
CollectionAssert.AreEqual(expectedSamplesStandardDeviationNumerators, outputSamplesStandardDeviationNumerators);
expectedSamplesStandardDeviationNumerators[0] = 0;
expectedSamplesStandardDeviationNumerators[1] = Math.Pow(22.729 - samplesMeanIntensityValue[1], 2)
+ Math.Pow(25.535 - samplesMeanIntensityValue[1], 2);
expectedSamplesStandardDeviationNumerators[2] = Math.Pow(22.347 - samplesMeanIntensityValue[2], 2);
expectedSamplesStandardDeviationNumerators[3] = Math.Pow(22.397 - samplesMeanIntensityValue[3], 2);
expectedSamplesStandardDeviationNumerators[4] = Math.Pow(25.370 - samplesMeanIntensityValue[4], 2);
expectedSamplesStandardDeviationNumerators[5] = Math.Pow(23.180 - samplesMeanIntensityValue[5], 2)
+ Math.Pow(24 - samplesMeanIntensityValue[5], 2);
expectedSamplesStandardDeviationNumerators[6] = Math.Pow(23.293 - samplesMeanIntensityValue[6], 2)
+ Math.Pow(25.359 - samplesMeanIntensityValue[6], 2);
expectedSamplesStandardDeviationNumerators[7] = 0;
imputationProcess.CalculateSampleStandardDeviationNumerator(sampleprotein2, outputSamplesStandardDeviationNumerators,
sampleFileNames, samplesMeanIntensityValue);
CollectionAssert.AreEqual(expectedSamplesStandardDeviationNumerators, outputSamplesStandardDeviationNumerators);
}