private void defineClassesToolStripMenuItem_Click(object sender, EventArgs e)
{
cGUI_2ClassesSelection GUI_ListClasses = new cGUI_2ClassesSelection();
GUI_ListClasses.ClassType = eClassType.WELL;
if (GUI_ListClasses.Run(this.GlobalInfo).IsSucceed == false) return;
cExtendedTable ListClassSelected = GUI_ListClasses.GetOutPut();
for (int i = 0; i < ListClassSelected[0].Count; i++)
if (ListClassSelected[0][i] > 0) cGlobalInfo.CtrlNeg = cGlobalInfo.ListWellClasses[i];
for (int i = 0; i < ListClassSelected[1].Count; i++)
if (ListClassSelected[1][i] > 0) cGlobalInfo.CtrlPos = cGlobalInfo.ListWellClasses[i];
cGlobalInfo.WindowHCSAnalyzer.UpdateQCDisplay();
}
private void mannWithneyTestToolStripMenuItem_Click(object sender, EventArgs e)
{
cGUI_2ClassesSelection GUI_ListClasses = new cGUI_2ClassesSelection();
if (GUI_ListClasses.Run(this.GlobalInfo).IsSucceed == false) return;
cExtendedTable ListClassSelected = GUI_ListClasses.GetOutPut();
int IdxClassNeg = -1;
int IdxClassPos = -1;
for (int IdxC = 0; IdxC < ListClassSelected[0].Count; IdxC++)
{
if (ListClassSelected[0][IdxC] == 1) IdxClassNeg = IdxC;
if (ListClassSelected[1][IdxC] == 1) IdxClassPos = IdxC;
}
string SubTitle = "Mann-Withney (both tails)";
#region single plate and plate by plate
cDesignerTab DT = new cDesignerTab();
if ((ProcessModeCurrentPlateOnlyToolStripMenuItem.Checked) || (ProcessModeplateByPlateToolStripMenuItem.Checked)/*||(ProcessModeEntireScreeningToolStripMenuItem.Checked)*/)
{
List<cPlate> ListPlatesToProcess = new List<cPlate>();
if ((ProcessModeplateByPlateToolStripMenuItem.Checked)/*||(ProcessModeEntireScreeningToolStripMenuItem.Checked)*/)
{
foreach (cPlate TmpPlate in cGlobalInfo.CurrentScreening.ListPlatesActive)
ListPlatesToProcess.Add(TmpPlate);
}
else
ListPlatesToProcess.Add(cGlobalInfo.CurrentScreening.GetCurrentDisplayPlate());
foreach (cPlate TmpPlate in ListPlatesToProcess)
{
cListWells ListWellsToProcess1 = new cListWells(null);
cListWells ListWellsToProcess2 = new cListWells(null);
foreach (cWell item in TmpPlate.ListActiveWells)
{
if (item.GetCurrentClassIdx() != -1)
{
if (ListClassSelected[0][item.GetCurrentClassIdx()] == 1)
ListWellsToProcess1.Add(item);
if (ListClassSelected[1][item.GetCurrentClassIdx()] == 1)
ListWellsToProcess2.Add(item);
}
}
cExtendedTable NewTable1 = new cExtendedTable(ListWellsToProcess1, true);
cExtendedTable NewTable2 = new cExtendedTable(ListWellsToProcess2, true);
if ((NewTable1.Count == 0) || (NewTable1[0].Count < 5) || (NewTable2.Count == 0) || (NewTable2[0].Count < 5))
{
if (ProcessModeCurrentPlateOnlyToolStripMenuItem.Checked)
{
MessageBox.Show("Insufficient number of control wells", "Error", MessageBoxButtons.OK, MessageBoxIcon.Error);
return;
}
else
continue;
}
cExtendedList ListZ = new cExtendedList();
List<cDescriptorType> ListDescForZFactor = new List<cDescriptorType>();
List<string> ListNames = new List<string>();
int RealIdx = 0;
for (int IDxDesc = 0; IDxDesc < cGlobalInfo.CurrentScreening.ListDescriptors.Count; IDxDesc++)
{
if (!cGlobalInfo.CurrentScreening.ListDescriptors[IDxDesc].IsActive()) continue;
cExtendedTable TableForZ = new cExtendedTable();
TableForZ.Add(NewTable1[RealIdx]);
TableForZ.Add(NewTable2[RealIdx]);
RealIdx++;
cMannWithneyTest ZF = new cMannWithneyTest();
ZF.SetInputData(TableForZ);
ZF.Run();
ListZ.Add(ZF.GetOutPut()[0][1]);
ListDescForZFactor.Add(cGlobalInfo.CurrentScreening.ListDescriptors[IDxDesc]);
}
cExtendedTable ET = new cExtendedTable(new cExtendedTable(ListZ));
ET[0].ListTags = new List<object>();
ET[0].ListTags.AddRange(ListDescForZFactor);
ET.Name = TmpPlate.GetName() + "\n" + SubTitle + " - " + cGlobalInfo.ListWellClasses[IdxClassNeg].Name + " (" + NewTable1[0].Count + " wells) vs. " + cGlobalInfo.ListWellClasses[IdxClassPos].Name + " (" + NewTable2[0].Count + " wells)";
ET[0].Name = ET.Name;
cSort S = new cSort();
S.SetInputData(ET);
S.ColumnIndexForSorting = 0;
S.Run();
//ZFactorList.Sort(delegate(cSimpleSignature p1, cSimpleSignature p2) { return p1.AverageValue.CompareTo(p2.AverageValue); });
cViewerGraph1D VG1 = new cViewerGraph1D();
VG1.SetInputData(S.GetOutPut());
VG1.Chart.LabelAxisY = SubTitle;
VG1.Chart.LabelAxisX = "Descriptor";
VG1.Chart.IsZoomableX = true;
VG1.Chart.IsBar = true;
VG1.Chart.IsBorder = true;
VG1.Chart.IsDisplayValues = true;
VG1.Chart.IsShadow = true;
VG1.Chart.MarkerSize = 4;
//VG1.Chart.Max
VG1.Title = TmpPlate.GetName();
Classes.Base_Classes.General.cLineHorizontalForGraph VLZ05 = new Classes.Base_Classes.General.cLineHorizontalForGraph(.05);
VLZ05.IsAllowMoving = true;
VG1.Chart.ListHorizontalLines.Add(VLZ05);
VG1.Run();
cDesignerSplitter DS = new cDesignerSplitter();
DS.Orientation = Orientation.Vertical;
DS.SetInputData(VG1.GetOutPut());
cSort S1 = new cSort();
S1.SetInputData(ET);
S1.ColumnIndexForSorting = 0;
S1.IsAscending = false;
S1.Run();
cViewerTable VT = new cViewerTable();
VT.SetInputData(S1.GetOutPut());
VT.DigitNumber = -1;
VT.Run();
DS.SetInputData(VT.GetOutPut());
DS.Title = TmpPlate.GetName();
DS.Run();
DT.SetInputData(DS.GetOutPut());
}
DT.Run();
cDisplayToWindow CDW = new cDisplayToWindow();
CDW.SetInputData(DT.GetOutPut());//VG1.GetOutPut());
if (ProcessModeCurrentPlateOnlyToolStripMenuItem.Checked)
CDW.Title = SubTitle + " - " + ListPlatesToProcess[0].GetName();
else
CDW.Title = SubTitle + " - " + ListPlatesToProcess.Count + " plates";
CDW.Run();
CDW.Display();
}
#endregion
#region entire screening
else if (ProcessModeEntireScreeningToolStripMenuItem.Checked)
{
List<cPlate> ListPlatesToProcess = new List<cPlate>();
foreach (cPlate TmpPlate in cGlobalInfo.CurrentScreening.ListPlatesActive)
ListPlatesToProcess.Add(TmpPlate);
cExtendedList ListZ = new cExtendedList();
List<cPlate> ListPlatesForZFactor = new List<cPlate>();
foreach (cPlate TmpPlate in ListPlatesToProcess)
{
cListWells ListWellsToProcess1 = new cListWells(null);
cListWells ListWellsToProcess2 = new cListWells(null);
foreach (cWell item in TmpPlate.ListActiveWells)
{
if (item.GetCurrentClassIdx() != -1)
{
if (ListClassSelected[0][item.GetCurrentClassIdx()] == 1)
ListWellsToProcess1.Add(item);
if (ListClassSelected[1][item.GetCurrentClassIdx()] == 1)
ListWellsToProcess2.Add(item);
}
}
cExtendedTable NewTable1 = new cExtendedTable(ListWellsToProcess1, cGlobalInfo.CurrentScreening.ListDescriptors.GetDescriptorIndex(cGlobalInfo.CurrentScreening.ListDescriptors.GetActiveDescriptor()));
cExtendedTable NewTable2 = new cExtendedTable(ListWellsToProcess2, cGlobalInfo.CurrentScreening.ListDescriptors.GetDescriptorIndex(cGlobalInfo.CurrentScreening.ListDescriptors.GetActiveDescriptor()));
//cExtendedTable NewTable2 = new cExtendedTable(ListWellsToProcess2, false);
if ((NewTable1.Count == 0) || (NewTable1[0].Count < 5) || (NewTable2.Count == 0) || (NewTable2[0].Count < 5))
{
if (ProcessModeCurrentPlateOnlyToolStripMenuItem.Checked)
{
MessageBox.Show("Insufficient number of control wells", "Error", MessageBoxButtons.OK, MessageBoxIcon.Error);
return;
}
else
continue;
}
cExtendedTable TableForZ = new cExtendedTable();
TableForZ.Add(NewTable1[0]);
TableForZ.Add(NewTable2[0]);
cMannWithneyTest ZF = new cMannWithneyTest();
ZF.SetInputData(TableForZ);
ZF.Run();
double Zfactor = ZF.GetOutPut()[0][1];
ListZ.Add(Zfactor);
// update plate quality
//TmpPlate.ListProperties.UpdateValueByName("Quality", Math.Exp(Zfactor - 1));
//cProperty Prop = TmpPlate.ListProperties.FindByName("Quality");
//Prop.Info = ZF.GetInfo();
ListPlatesForZFactor.Add(TmpPlate);
}
cExtendedTable ET = new cExtendedTable(new cExtendedTable(ListZ));
ET[0].ListTags = new List<object>();
ET[0].ListTags.AddRange(ListPlatesForZFactor);
ET.Name = SubTitle + " - " + cGlobalInfo.CurrentScreening.ListDescriptors[cGlobalInfo.CurrentScreening.ListDescriptors.CurrentSelectedDescriptorIdx].GetName() + " - " + cGlobalInfo.ListWellClasses[IdxClassNeg].Name + /*" (" + NewTable1[0].Count + " wells)*/ " vs. " + cGlobalInfo.ListWellClasses[IdxClassPos].Name;// +" (" + NewTable2[0].Count + " wells)";
ET[0].Name = ET.Name;
cViewerGraph1D VG1 = new cViewerGraph1D();
VG1.SetInputData(ET);
VG1.Chart.LabelAxisY = SubTitle;
VG1.Chart.LabelAxisX = "Plate";
VG1.Chart.IsZoomableX = true;
VG1.Chart.IsBar = true;
VG1.Chart.IsBorder = true;
VG1.Chart.IsDisplayValues = true;
VG1.Chart.IsShadow = true;
VG1.Chart.MarkerSize = 4;
Classes.Base_Classes.General.cLineHorizontalForGraph VLZ05 = new Classes.Base_Classes.General.cLineHorizontalForGraph(.05);
VLZ05.IsAllowMoving = true;
VG1.Chart.ListHorizontalLines.Add(VLZ05);
VG1.Title = cGlobalInfo.CurrentScreening.ListDescriptors[cGlobalInfo.CurrentScreening.ListDescriptors.CurrentSelectedDescriptorIdx].GetName();
VG1.Run();
cDisplayToWindow CDW = new cDisplayToWindow();
CDW.SetInputData(VG1.GetOutPut());
CDW.Title = SubTitle + " - " + ListPlatesToProcess.Count + " plates";
CDW.Run();
CDW.Display();
#endregion
}
}
private void zScoreToolStripMenuItem_Click(object sender, EventArgs e)
{
#region obsolete
//List<double> Pos = new List<double>();
//List<double> Neg = new List<double>();
//List<cSimpleSignature> ZFactorList = new List<cSimpleSignature>();
//int NumDesc = CompleteScreening.ListDescriptors.Count;
//cWell TempWell;
//// loop on all the desciptors
//for (int Desc = 0; Desc < NumDesc; Desc++)
//{
// Pos.Clear();
// Neg.Clear();
// if (CompleteScreening.ListDescriptors[Desc].IsActive() == false) continue;
// for (int row = 0; row < CompleteScreening.Rows; row++)
// for (int col = 0; col < CompleteScreening.Columns; col++)
// {
// TempWell = CompleteScreening.GetCurrentDisplayPlate().GetWell(col, row, true);
// if (TempWell == null) continue;
// else
// {
// if (TempWell.GetClassIdx() == 0)
// Pos.Add(TempWell.ListDescriptors[Desc].GetValue());
// if (TempWell.GetClassIdx() == 1)
// Neg.Add(TempWell.ListDescriptors[Desc].GetValue());
// }
// }
// if (Pos.Count < 3)
// {
// MessageBox.Show("No or not enough positive controls !", "Warning", MessageBoxButtons.OK, MessageBoxIcon.Exclamation);
// return;
// }
// if (Neg.Count < 3)
// {
// MessageBox.Show("No or not enough negative controls !", "Warning", MessageBoxButtons.OK, MessageBoxIcon.Exclamation);
// return;
// }
// double ZScore = 1 - 3 * (std(Pos.ToArray()) + std(Neg.ToArray())) / (Math.Abs(Mean(Pos.ToArray()) - Mean(Neg.ToArray())));
// GlobalInfo.ConsoleWriteLine(CompleteScreening.ListDescriptors[Desc].GetName() + ", Z-Score = " + ZScore);
// cSimpleSignature TmpDesc = new cSimpleSignature(CompleteScreening.ListDescriptors[Desc].GetName(), ZScore);
// ZFactorList.Add(TmpDesc);
//}
//ZFactorList.Sort(delegate(cSimpleSignature p1, cSimpleSignature p2) { return p1.AverageValue.CompareTo(p2.AverageValue); });
//Series CurrentSeries = new Series();
//CurrentSeries.ChartType = SeriesChartType.Column;
//CurrentSeries.ShadowOffset = 1;
//Series SeriesLine = new Series();
//SeriesLine.Name = "SeriesLine";
//SeriesLine.ShadowOffset = 1;
//SeriesLine.ChartType = SeriesChartType.Line;
//int RealIdx = 0;
//for (int IdxValue = 0; IdxValue < ZFactorList.Count; IdxValue++)
//{
// if (double.IsNaN(ZFactorList[IdxValue].AverageValue)) continue;
// if (double.IsInfinity(ZFactorList[IdxValue].AverageValue)) continue;
// CurrentSeries.Points.Add(ZFactorList[IdxValue].AverageValue);
// CurrentSeries.Points[RealIdx].Label = ZFactorList[IdxValue].AverageValue.ToString("N2");
// CurrentSeries.Points[RealIdx].Font = new Font("Arial", 10);
// CurrentSeries.Points[RealIdx].ToolTip = ZFactorList[IdxValue].Name;
// CurrentSeries.Points[RealIdx].AxisLabel = ZFactorList[IdxValue].Name;
// SeriesLine.Points.Add(ZFactorList[IdxValue].AverageValue);
// SeriesLine.Points[RealIdx].BorderColor = Color.Black;
// SeriesLine.Points[RealIdx].MarkerStyle = MarkerStyle.Circle;
// SeriesLine.Points[RealIdx].MarkerSize = 4;
// RealIdx++;
//}
//SimpleForm NewWindow = new SimpleForm(CompleteScreening);
//int thisWidth = 200 * RealIdx;
//if (thisWidth > (int)GlobalInfo.OptionsWindow.numericUpDownMaximumWidth.Value) thisWidth = (int)GlobalInfo.OptionsWindow.numericUpDownMaximumWidth.Value;
//NewWindow.Width = thisWidth;
//NewWindow.Height = 400;
//NewWindow.Text = "Z-factors";
//ChartArea CurrentChartArea = new ChartArea();
//CurrentChartArea.BorderColor = Color.Black;
//CurrentChartArea.AxisX.Interval = 1;
//NewWindow.chartForSimpleForm.Series.Add(CurrentSeries);
//NewWindow.chartForSimpleForm.Series.Add(SeriesLine);
//CurrentChartArea.AxisX.IsLabelAutoFit = true;
//NewWindow.chartForSimpleForm.ChartAreas.Add(CurrentChartArea);
//CurrentChartArea.Axes[1].Maximum = 2;
//CurrentChartArea.Axes[1].IsMarksNextToAxis = true;
//CurrentChartArea.Axes[0].MajorGrid.Enabled = false;
//CurrentChartArea.Axes[1].MajorGrid.Enabled = false;
//NewWindow.chartForSimpleForm.TextAntiAliasingQuality = TextAntiAliasingQuality.High;
//CurrentChartArea.BackGradientStyle = GradientStyle.TopBottom;
//CurrentChartArea.BackColor = CompleteScreening.GlobalInfo.OptionsWindow.panel1.BackColor;
//CurrentChartArea.BackSecondaryColor = Color.White;
//CurrentChartArea.AxisX.ScaleView.Zoomable = true;
//CurrentChartArea.AxisY.ScaleView.Zoomable = true;
//Title CurrentTitle = new Title(CompleteScreening.GetCurrentDisplayPlate().Name + " Z-factors");
//CurrentTitle.Font = new System.Drawing.Font("Arial", 11, FontStyle.Bold);
//NewWindow.chartForSimpleForm.Titles.Add(CurrentTitle);
//NewWindow.Show();
//NewWindow.chartForSimpleForm.Update();
//NewWindow.chartForSimpleForm.Show();
//NewWindow.AutoScroll = true;
//NewWindow.Controls.AddRange(new System.Windows.Forms.Control[] { NewWindow.chartForSimpleForm });
#endregion
cGUI_2ClassesSelection GUI_ListClasses = new cGUI_2ClassesSelection();
if (ProcessModeCurrentPlateOnlyToolStripMenuItem.Checked)
{
GUI_ListClasses.PanelRight_IsCheckBoxes = true;
}
if (GUI_ListClasses.Run(this.GlobalInfo).IsSucceed == false) return;
cExtendedTable ListClassSelected = GUI_ListClasses.GetOutPut();
int IdxClassNeg = -1;
int IdxClassPos = -1;
for (int IdxC = 0; IdxC < ListClassSelected[0].Count; IdxC++)
{
if (ListClassSelected[0][IdxC] == 1) IdxClassNeg = IdxC;
if (ListClassSelected[1][IdxC] == 1) IdxClassPos = IdxC;
}
string SubTitle = "Z-Factor";
if (_QCZRobustItem.Checked)
SubTitle += " (Robust)";
#region Single plate
cDesignerTab DT = new cDesignerTab();
// cDesignerMultiChoices DT = new cDesignerMultiChoices();
if ((ProcessModeCurrentPlateOnlyToolStripMenuItem.Checked)/*||(ProcessModeEntireScreeningToolStripMenuItem.Checked)*/)
{
List<cPlate> ListPlatesToProcess = new List<cPlate>();
cPlate TmpPlate = cGlobalInfo.CurrentScreening.GetCurrentDisplayPlate();
// foreach (cPlate TmpPlate in ListPlatesToProcess)
// GUI_ListClasses.Get
// ListClassSelected
List<int> ListCheckBoxes = new List<int>();
for (int i = 0; i < ListClassSelected[1].Count; i++)
{
if (ListClassSelected[1][i] > 0)
ListCheckBoxes.Add(i);
}
for (int IdxClassSelected = 0; IdxClassSelected < ListCheckBoxes.Count; IdxClassSelected++)
{
cListWells ListWellsToProcess1 = new cListWells(null);
cListWells ListWellsToProcess2 = new cListWells(null);
int CurrentClassTobeProcessed = ListCheckBoxes[IdxClassSelected];
foreach (cWell item in TmpPlate.ListActiveWells)
{
if (item.GetCurrentClassIdx() != -1)
{
if (ListClassSelected[0][item.GetCurrentClassIdx()] == 1)
ListWellsToProcess1.Add(item);
if (item.GetCurrentClassIdx() == CurrentClassTobeProcessed)
// if (ListClassSelected[1][ListCheckBoxes[IdxClassSelected]] == 1)
ListWellsToProcess2.Add(item);
}
}
cExtendedTable NewTable1 = new cExtendedTable(ListWellsToProcess1, true);
cExtendedTable NewTable2 = new cExtendedTable(ListWellsToProcess2, true);
if ((NewTable1.Count == 0) || (NewTable1[0].Count < 2) || (NewTable2.Count == 0) || (NewTable2[0].Count < 2))
{
if (ProcessModeCurrentPlateOnlyToolStripMenuItem.Checked)
{
MessageBox.Show("Insufficient number of control wells", "Error", MessageBoxButtons.OK, MessageBoxIcon.Error);
return;
}
//else
// continue;
}
cExtendedList ListZ = new cExtendedList();
List<cDescriptorType> ListDescForZFactor = new List<cDescriptorType>();
List<string> ListNames = new List<string>();
int RealIdx = 0;
for (int IDxDesc = 0; IDxDesc < cGlobalInfo.CurrentScreening.ListDescriptors.Count; IDxDesc++)
{
if (!cGlobalInfo.CurrentScreening.ListDescriptors[IDxDesc].IsActive()) continue;
cExtendedTable TableForZ = new cExtendedTable();
TableForZ.Add(NewTable1[RealIdx]);
TableForZ.Add(NewTable2[RealIdx]);
RealIdx++;
cZFactor ZF = new cZFactor();
ZF.SetInputData(TableForZ);
ZF.IsRobust = _QCZRobustItem.Checked;
ZF.Run();
ListZ.Add(ZF.GetOutPut()[0][1]);
ListDescForZFactor.Add(cGlobalInfo.CurrentScreening.ListDescriptors[IDxDesc]);
}
cExtendedTable ET = new cExtendedTable(new cExtendedTable(ListZ));
ET[0].ListTags = new List<object>();
ET[0].ListTags.AddRange(ListDescForZFactor);
ET.Name = TmpPlate.GetName() + "\n" + SubTitle + " - " + cGlobalInfo.ListWellClasses[IdxClassNeg].Name + " (" + NewTable1[0].Count + " wells) vs. " + cGlobalInfo.ListWellClasses[CurrentClassTobeProcessed].Name + " (" + NewTable2[0].Count + " wells)";
ET[0].Name = ET.Name;
cSort S = new cSort();
S.SetInputData(ET);
S.ColumnIndexForSorting = 0;
S.Run();
//ZFactorList.Sort(delegate(cSimpleSignature p1, cSimpleSignature p2) { return p1.AverageValue.CompareTo(p2.AverageValue); });
cViewerGraph1D VG1 = new cViewerGraph1D();
VG1.SetInputData(S.GetOutPut());
VG1.Chart.LabelAxisY = SubTitle;
VG1.Chart.LabelAxisX = "Descriptor";
VG1.Chart.IsZoomableX = true;
VG1.Chart.IsBar = true;
VG1.Chart.IsBorder = true;
VG1.Chart.IsDisplayValues = true;
VG1.Chart.IsShadow = true;
VG1.Chart.MarkerSize = 4;
VG1.Chart.DefaultAxisYMax = new cExtendedList();
VG1.Chart.DefaultAxisYMax.Add(1);
if (!cGlobalInfo.OptionsWindow.FFAllOptions.checkBoxZscoreMinValueAutomated.Checked)
{
VG1.Chart.DefaultAxisYMin = new cExtendedList();
VG1.Chart.DefaultAxisYMin.Add((double)cGlobalInfo.OptionsWindow.FFAllOptions.numericUpDownZscoreMinValue.Value);
}
VG1.Title = TmpPlate.GetName();
Classes.Base_Classes.General.cLineHorizontalForGraph VLZ05 = new Classes.Base_Classes.General.cLineHorizontalForGraph(.5);
VLZ05.IsAllowMoving = true;
VG1.Chart.ListHorizontalLines.Add(VLZ05);
VG1.Run();
cDesignerSplitter DS = new cDesignerSplitter();
DS.Orientation = Orientation.Vertical;
DS.SetInputData(VG1.GetOutPut());
cSort S1 = new cSort();
S1.SetInputData(ET);
S1.ColumnIndexForSorting = 0;
S1.IsAscending = false;
S1.Run();
cViewerTable VT = new cViewerTable();
VT.SetInputData(S1.GetOutPut());
VT.Run();
DS.SetInputData(VT.GetOutPut());
DS.Title = cGlobalInfo.ListWellClasses[IdxClassNeg].Name + " vs. " + cGlobalInfo.ListWellClasses[CurrentClassTobeProcessed].Name;
DS.Run();
DT.SetInputData(DS.GetOutPut());
}
DT.Run();
cDisplayToWindow CDW = new cDisplayToWindow();
CDW.SetInputData(DT.GetOutPut());//VG1.GetOutPut());
CDW.Title = SubTitle + " - " + TmpPlate.GetName();
CDW.Run();
CDW.Display();
}
#endregion
#region plate by plate
DT = new cDesignerTab();
// cDesignerMultiChoices DT = new cDesignerMultiChoices();
if ((ProcessModeplateByPlateToolStripMenuItem.Checked)/*||(ProcessModeEntireScreeningToolStripMenuItem.Checked)*/)
{
List<cPlate> ListPlatesToProcess = new List<cPlate>();
if ((ProcessModeplateByPlateToolStripMenuItem.Checked)/*||(ProcessModeEntireScreeningToolStripMenuItem.Checked)*/)
{
foreach (cPlate TmpPlate in cGlobalInfo.CurrentScreening.ListPlatesActive)
ListPlatesToProcess.Add(TmpPlate);
}
foreach (cPlate TmpPlate in ListPlatesToProcess)
{
cListWells ListWellsToProcess1 = new cListWells(null);
cListWells ListWellsToProcess2 = new cListWells(null);
foreach (cWell item in TmpPlate.ListActiveWells)
{
if (item.GetCurrentClassIdx() != -1)
{
if (ListClassSelected[0][item.GetCurrentClassIdx()] == 1)
ListWellsToProcess1.Add(item);
if (ListClassSelected[1][item.GetCurrentClassIdx()] == 1)
ListWellsToProcess2.Add(item);
}
}
cExtendedTable NewTable1 = new cExtendedTable(ListWellsToProcess1, true);
cExtendedTable NewTable2 = new cExtendedTable(ListWellsToProcess2, true);
if ((NewTable1.Count == 0) || (NewTable1[0].Count < 2) || (NewTable2.Count == 0) || (NewTable2[0].Count < 2))
{
if (ProcessModeCurrentPlateOnlyToolStripMenuItem.Checked)
{
MessageBox.Show("Insufficient number of control wells", "Error", MessageBoxButtons.OK, MessageBoxIcon.Error);
return;
}
else
continue;
}
cExtendedList ListZ = new cExtendedList();
List<cDescriptorType> ListDescForZFactor = new List<cDescriptorType>();
List<string> ListNames = new List<string>();
int RealIdx = 0;
for (int IDxDesc = 0; IDxDesc < cGlobalInfo.CurrentScreening.ListDescriptors.Count; IDxDesc++)
{
if (!cGlobalInfo.CurrentScreening.ListDescriptors[IDxDesc].IsActive()) continue;
cExtendedTable TableForZ = new cExtendedTable();
TableForZ.Add(NewTable1[RealIdx]);
TableForZ.Add(NewTable2[RealIdx]);
RealIdx++;
cZFactor ZF = new cZFactor();
ZF.SetInputData(TableForZ);
ZF.IsRobust = _QCZRobustItem.Checked;
ZF.Run();
ListZ.Add(ZF.GetOutPut()[0][1]);
ListDescForZFactor.Add(cGlobalInfo.CurrentScreening.ListDescriptors[IDxDesc]);
}
cExtendedTable ET = new cExtendedTable(new cExtendedTable(ListZ));
ET[0].ListTags = new List<object>();
ET[0].ListTags.AddRange(ListDescForZFactor);
ET.Name = TmpPlate.GetName() + "\n" + SubTitle + " - " + cGlobalInfo.ListWellClasses[IdxClassNeg].Name + " (" + NewTable1[0].Count + " wells) vs. " + cGlobalInfo.ListWellClasses[IdxClassPos].Name + " (" + NewTable2[0].Count + " wells)";
ET[0].Name = ET.Name;
cSort S = new cSort();
S.SetInputData(ET);
S.ColumnIndexForSorting = 0;
S.Run();
//ZFactorList.Sort(delegate(cSimpleSignature p1, cSimpleSignature p2) { return p1.AverageValue.CompareTo(p2.AverageValue); });
cViewerGraph1D VG1 = new cViewerGraph1D();
VG1.SetInputData(S.GetOutPut());
VG1.Chart.LabelAxisY = SubTitle;
VG1.Chart.LabelAxisX = "Descriptor";
VG1.Chart.IsZoomableX = true;
VG1.Chart.IsBar = true;
VG1.Chart.IsBorder = true;
VG1.Chart.IsDisplayValues = true;
VG1.Chart.IsShadow = true;
VG1.Chart.MarkerSize = 4;
VG1.Chart.DefaultAxisYMax = new cExtendedList();
VG1.Chart.DefaultAxisYMax.Add(1);
if (!cGlobalInfo.OptionsWindow.FFAllOptions.checkBoxZscoreMinValueAutomated.Checked)
{
VG1.Chart.DefaultAxisYMin = new cExtendedList();
VG1.Chart.DefaultAxisYMin.Add((double)cGlobalInfo.OptionsWindow.FFAllOptions.numericUpDownZscoreMinValue.Value);
}
VG1.Title = TmpPlate.GetName();
Classes.Base_Classes.General.cLineHorizontalForGraph VLZ05 = new Classes.Base_Classes.General.cLineHorizontalForGraph(.5);
VLZ05.IsAllowMoving = true;
VG1.Chart.ListHorizontalLines.Add(VLZ05);
VG1.Run();
cDesignerSplitter DS = new cDesignerSplitter();
DS.Orientation = Orientation.Vertical;
DS.SetInputData(VG1.GetOutPut());
cSort S1 = new cSort();
S1.SetInputData(ET);
S1.ColumnIndexForSorting = 0;
S1.IsAscending = false;
S1.Run();
cViewerTable VT = new cViewerTable();
VT.SetInputData(S1.GetOutPut());
VT.Run();
DS.SetInputData(VT.GetOutPut());
DS.Title = TmpPlate.GetName();
DS.Run();
DT.SetInputData(DS.GetOutPut());
}
DT.Run();
cDisplayToWindow CDW = new cDisplayToWindow();
CDW.SetInputData(DT.GetOutPut());//VG1.GetOutPut());
CDW.Title = SubTitle + " - " + ListPlatesToProcess.Count + " plates";
CDW.Run();
CDW.Display();
}
#endregion
#region entire screening
else if (ProcessModeEntireScreeningToolStripMenuItem.Checked)
{
List<cPlate> ListPlatesToProcess = new List<cPlate>();
foreach (cPlate TmpPlate in cGlobalInfo.CurrentScreening.ListPlatesActive)
ListPlatesToProcess.Add(TmpPlate);
cExtendedList ListZ = new cExtendedList();
List<cPlate> ListPlatesForZFactor = new List<cPlate>();
foreach (cPlate TmpPlate in ListPlatesToProcess)
{
cListWells ListWellsToProcess1 = new cListWells(null);
cListWells ListWellsToProcess2 = new cListWells(null);
foreach (cWell item in TmpPlate.ListActiveWells)
{
if (item.GetCurrentClassIdx() != -1)
{
if (ListClassSelected[0][item.GetCurrentClassIdx()] == 1)
ListWellsToProcess1.Add(item);
if (ListClassSelected[1][item.GetCurrentClassIdx()] == 1)
ListWellsToProcess2.Add(item);
}
}
cExtendedTable NewTable1 = new cExtendedTable(ListWellsToProcess1, cGlobalInfo.CurrentScreening.ListDescriptors.GetDescriptorIndex(cGlobalInfo.CurrentScreening.ListDescriptors.GetActiveDescriptor()));
cExtendedTable NewTable2 = new cExtendedTable(ListWellsToProcess2, cGlobalInfo.CurrentScreening.ListDescriptors.GetDescriptorIndex(cGlobalInfo.CurrentScreening.ListDescriptors.GetActiveDescriptor()));
//cExtendedTable NewTable2 = new cExtendedTable(ListWellsToProcess2, false);
if ((NewTable1.Count == 0) || (NewTable1[0].Count < 2) || (NewTable2.Count == 0) || (NewTable2[0].Count < 2))
{
if (ProcessModeCurrentPlateOnlyToolStripMenuItem.Checked)
{
MessageBox.Show("Insufficient number of control wells", "Error", MessageBoxButtons.OK, MessageBoxIcon.Error);
return;
}
else
continue;
}
cExtendedTable TableForZ = new cExtendedTable();
TableForZ.Add(NewTable1[0]);
TableForZ.Add(NewTable2[0]);
cZFactor ZF = new cZFactor();
ZF.IsRobust = _QCZRobustItem.Checked;
ZF.SetInputData(TableForZ);
ZF.Run();
double Zfactor = ZF.GetOutPut()[0][1];
ListZ.Add(Zfactor);
// update plate quality
TmpPlate.ListProperties.UpdateValueByName("Quality", Math.Exp(Zfactor - 1));
cProperty Prop = TmpPlate.ListProperties.FindByName("Quality");
Prop.Info = ZF.GetInfo();
ListPlatesForZFactor.Add(TmpPlate);
}
cExtendedTable ET = new cExtendedTable(new cExtendedTable(ListZ));
ET[0].ListTags = new List<object>();
ET[0].ListTags.AddRange(ListPlatesForZFactor);
ET.Name = SubTitle + " - " + cGlobalInfo.CurrentScreening.ListDescriptors[cGlobalInfo.CurrentScreening.ListDescriptors.CurrentSelectedDescriptorIdx].GetName() + " - " + cGlobalInfo.ListWellClasses[IdxClassNeg].Name + /*" (" + NewTable1[0].Count + " wells)*/ " vs. " + cGlobalInfo.ListWellClasses[IdxClassPos].Name;// +" (" + NewTable2[0].Count + " wells)";
ET[0].Name = ET.Name;
cViewerGraph1D VG1 = new cViewerGraph1D();
VG1.SetInputData(ET);
VG1.Chart.LabelAxisY = SubTitle;
VG1.Chart.LabelAxisX = "Plate";
VG1.Chart.IsZoomableX = true;
VG1.Chart.IsBar = true;
VG1.Chart.IsBorder = true;
VG1.Chart.IsDisplayValues = true;
VG1.Chart.IsShadow = true;
VG1.Chart.MarkerSize = 4;
VG1.Chart.DefaultAxisYMax = new cExtendedList();
VG1.Chart.DefaultAxisYMax.Add(1);
if (!cGlobalInfo.OptionsWindow.FFAllOptions.checkBoxZscoreMinValueAutomated.Checked)
{
VG1.Chart.DefaultAxisYMin = new cExtendedList();
VG1.Chart.DefaultAxisYMin.Add((double)cGlobalInfo.OptionsWindow.FFAllOptions.numericUpDownZscoreMinValue.Value);
}
Classes.Base_Classes.General.cLineHorizontalForGraph VLZ05 = new Classes.Base_Classes.General.cLineHorizontalForGraph(.5);
VLZ05.IsAllowMoving = true;
VG1.Chart.ListHorizontalLines.Add(VLZ05);
VG1.Title = cGlobalInfo.CurrentScreening.ListDescriptors[cGlobalInfo.CurrentScreening.ListDescriptors.CurrentSelectedDescriptorIdx].GetName();
VG1.Run();
cDisplayToWindow CDW = new cDisplayToWindow();
CDW.SetInputData(VG1.GetOutPut());
CDW.Title = SubTitle + " - " + ListPlatesToProcess.Count + " plates";
CDW.Run();
CDW.Display();
#endregion
}
}
private void ToolStripMenuItem_ZFactor(object sender, EventArgs e)
{
cGUI_2ClassesSelection GUI_ListClasses = new cGUI_2ClassesSelection();
if (GUI_ListClasses.Run(this.GlobalInfo).IsSucceed == false) return;
cExtendedTable ListClassSelected = GUI_ListClasses.GetOutPut();
int IdxClassNeg = -1;
int IdxClassPos = -1;
for (int IdxC = 0; IdxC < ListClassSelected[0].Count; IdxC++)
{
if (ListClassSelected[0][IdxC] == 1) IdxClassNeg = IdxC;
if (ListClassSelected[1][IdxC] == 1) IdxClassPos = IdxC;
}
string SubTitle = "Z-Factor";
cListWells ListWellsToProcess1 = new cListWells(null);
cListWells ListWellsToProcess2 = new cListWells(null);
foreach (cWell item in cGlobalInfo.ListSelectedWell)
{
if (item.GetCurrentClassIdx() != -1)
{
if (ListClassSelected[0][item.GetCurrentClassIdx()] == 1)
ListWellsToProcess1.Add(item);
if (ListClassSelected[1][item.GetCurrentClassIdx()] == 1)
ListWellsToProcess2.Add(item);
}
}
cExtendedTable NewTable1 = new cExtendedTable(ListWellsToProcess1, true);
cExtendedTable NewTable2 = new cExtendedTable(ListWellsToProcess2, true);
if ((NewTable1.Count == 0) || (NewTable1[0].Count < 2) || (NewTable2.Count == 0) || (NewTable2[0].Count < 2))
{
if (ProcessModeCurrentPlateOnlyToolStripMenuItem.Checked)
{
MessageBox.Show("Insufficient number of control wells", "Error", MessageBoxButtons.OK, MessageBoxIcon.Error);
return;
}
}
cExtendedList ListZ = new cExtendedList();
List<cDescriptorType> ListDescForZFactor = new List<cDescriptorType>();
List<string> ListNames = new List<string>();
int RealIdx = 0;
for (int IDxDesc = 0; IDxDesc < cGlobalInfo.CurrentScreening.ListDescriptors.Count; IDxDesc++)
{
if (!cGlobalInfo.CurrentScreening.ListDescriptors[IDxDesc].IsActive()) continue;
cExtendedTable TableForZ = new cExtendedTable();
TableForZ.Add(NewTable1[RealIdx]);
TableForZ.Add(NewTable2[RealIdx]);
RealIdx++;
cZFactor ZF = new cZFactor();
ZF.SetInputData(TableForZ);
ZF.IsRobust = _QCZRobustItem.Checked;
ZF.Run();
ListZ.Add(ZF.GetOutPut()[0][1]);
ListDescForZFactor.Add(cGlobalInfo.CurrentScreening.ListDescriptors[IDxDesc]);
}
cExtendedTable ET = new cExtendedTable(new cExtendedTable(ListZ));
ET[0].ListTags = new List<object>();
ET[0].ListTags.AddRange(ListDescForZFactor);
ET.Name = SubTitle + " - " + cGlobalInfo.ListWellClasses[IdxClassNeg].Name + " (" + NewTable1[0].Count + " wells) vs. " + cGlobalInfo.ListWellClasses[IdxClassPos].Name + " (" + NewTable2[0].Count + " wells)";
ET[0].Name = ET.Name;
cSort S = new cSort();
S.SetInputData(ET);
S.ColumnIndexForSorting = 0;
S.Run();
cViewerGraph1D VG1 = new cViewerGraph1D();
VG1.SetInputData(S.GetOutPut());
VG1.Chart.LabelAxisY = SubTitle;
VG1.Chart.LabelAxisX = "Descriptor";
VG1.Chart.IsZoomableX = true;
VG1.Chart.IsBar = true;
VG1.Chart.IsBorder = true;
VG1.Chart.IsDisplayValues = true;
VG1.Chart.IsShadow = true;
VG1.Chart.MarkerSize = 4;
VG1.Chart.DefaultAxisYMax = new cExtendedList();
VG1.Chart.DefaultAxisYMax.Add(1);
//VG1.Title = TmpPlate.Name;
Classes.Base_Classes.General.cLineHorizontalForGraph VLZ05 = new Classes.Base_Classes.General.cLineHorizontalForGraph(.5);
VLZ05.IsAllowMoving = true;
VG1.Chart.ListHorizontalLines.Add(VLZ05);
VG1.Run();
cDesignerSplitter DS = new cDesignerSplitter();
DS.Orientation = Orientation.Vertical;
DS.SetInputData(VG1.GetOutPut());
cSort S1 = new cSort();
S1.SetInputData(ET);
S1.ColumnIndexForSorting = 0;
S1.IsAscending = false;
S1.Run();
cViewerTable VT = new cViewerTable();
VT.SetInputData(S1.GetOutPut());
VT.Run();
DS.SetInputData(VT.GetOutPut());
DS.Run();
cDisplayToWindow CDW = new cDisplayToWindow();
CDW.SetInputData(DS.GetOutPut());//VG1.GetOutPut());
CDW.Title = "List Wells - Z Factor";
CDW.Run();
CDW.Display();
}
private void RatioPopDescItem(object sender, EventArgs e)
{
cGUI_2ClassesSelection GUI_ListClasses = new cGUI_2ClassesSelection();
GUI_ListClasses.ClassType = eClassType.PHENOTYPE;
GUI_ListClasses.PanelRight_IsCheckBoxes = true;
GUI_ListClasses.PanelLeft_IsCheckBoxes = true;
if (GUI_ListClasses.Run(this.GlobalInfo).IsSucceed == false) return;
List<cCellularPhenotype> LCP0 = new List<cCellularPhenotype>();
List<cCellularPhenotype> LCP1 = new List<cCellularPhenotype>();
for (int IdxPheno = 0; IdxPheno < GUI_ListClasses.GetOutPut()[0].Count; IdxPheno++)
{
if (GUI_ListClasses.GetOutPut()[0][IdxPheno] == 1)
LCP0.Add(cGlobalInfo.ListCellularPhenotypes[IdxPheno]);
if (GUI_ListClasses.GetOutPut()[1][IdxPheno] == 1)
LCP1.Add(cGlobalInfo.ListCellularPhenotypes[IdxPheno]);
}
//if (LCP.Count == 0) return;
string description = "Ratio of:\n";
foreach (var item in LCP0)
description += item.Name + "\n";
description += "over\n";
foreach (var item in LCP1)
description += item.Name + "\n";
cDescriptorType NewAverageType = new cDescriptorType("Ratio_Pop", true, 1, description);
cGlobalInfo.CurrentScreening.ListDescriptors.AddNew(NewAverageType);
FormForProgress ProgressWindow = new FormForProgress();
ProgressWindow.Show();
int IdxProgress = 0;
int MaxProgress = 0;
foreach (cPlate CurrentPlateToProcess in cGlobalInfo.CurrentScreening.ListPlatesAvailable)
MaxProgress += (int)CurrentPlateToProcess.ListWells.Count;
ProgressWindow.progressBar.Maximum = MaxProgress;
foreach (cPlate TmpPlate in cGlobalInfo.CurrentScreening.ListPlatesAvailable)
{
foreach (cWell Tmpwell in TmpPlate.ListWells)
{
TmpPlate.DBConnection = new cDBConnection(TmpPlate, Tmpwell.SQLTableName);
List<cDescriptorType> LDT = new List<cDescriptorType>();
LDT.Add(cGlobalInfo.CurrentScreening.ListDescriptors[0]);
cExtendedTable CT = TmpPlate.DBConnection.GetWellValues(Tmpwell, LDT, LCP0, 1);
double NumObject0 = CT[0].Count;
cExtendedTable CT1 = TmpPlate.DBConnection.GetWellValues(Tmpwell, LDT, LCP1, 1);
double NumObject1 = CT1[0].Count;
cListSignature LDesc = new cListSignature();
double Value = 0;
if (NumObject1 == 0)
Value = 0;
else
Value = NumObject0 / NumObject1;
cSignature NewDesc = new cSignature(Value, NewAverageType, cGlobalInfo.CurrentScreening);
LDesc.Add(NewDesc);
Tmpwell.AddSignatures(LDesc);
TmpPlate.DBConnection.CloseConnection();
ProgressWindow.richTextBoxForComment.AppendText(TmpPlate.GetName() + " : " + Tmpwell.GetShortInfo().Remove(Tmpwell.GetShortInfo().Length - 2) + "\n");
ProgressWindow.progressBar.Value = IdxProgress++;
ProgressWindow.Refresh();
}
}
ProgressWindow.Close();
cGlobalInfo.CurrentScreening.ListDescriptors.UpDateDisplay();
cGlobalInfo.CurrentScreening.UpDatePlateListWithFullAvailablePlate();
for (int idxP = 0; idxP < cGlobalInfo.CurrentScreening.ListPlatesActive.Count; idxP++)
cGlobalInfo.CurrentScreening.ListPlatesActive[idxP].UpDataMinMax();
}
private void ToolStripMenuItem_SwapPhenotype(object sender, EventArgs e)
{
cGUI_2ClassesSelection GUI_ListClasses = new cGUI_2ClassesSelection();
GUI_ListClasses.ClassType = eClassType.PHENOTYPE;
GUI_ListClasses.PanelLeft_IsCheckBoxes = true;
GUI_ListClasses.PanelRight_IsCheckBoxes = false;
GUI_ListClasses.Title = "Swap Phenotypes";
if (GUI_ListClasses.Run(this.GlobalInfo).IsSucceed == false) return;
int IdxNewPhenotype = 0;
for (int IdxPheno = 0; IdxPheno < GUI_ListClasses.GetOutPut()[0].Count; IdxPheno++)
{
if (GUI_ListClasses.GetOutPut()[1][IdxPheno] == 1)
IdxNewPhenotype = IdxPheno;
}
int IDxPt = 0;
foreach (DataPoint item in this.chartForPoints.Series[0].Points)
{
// if ((item.XValue >= MinX) && (item.XValue <= MaxX) && (item.YValues[0] >= MinY) && (item.YValues[0] <= MaxY))
// {
// if ((item.Tag != null) && (item.Tag.GetType() == typeof(cSingleBiologicalObject)))
{
if (GUI_ListClasses.GetOutPut()[0][(int)MachineLearning.Classes[IDxPt]/*((cSingleBiologicalObject)(item.Tag)).GetAssociatedPhenotype().Idx*/] == 1)
MachineLearning.Classes[IDxPt] = IdxNewPhenotype;
// LDP.Add(item);
}
// }
IDxPt++;
}
this.ReFreshMainGraph();
this.RedrawHistoHorizontal(true);
/*
int Idx = 0;
// int OriginalIdx = WindowSwapClasses.comboBoxOriginalClass.SelectedIndex - 1;
int DestinatonIdx = WindowSwapClasses.comboBoxDestinationClass.SelectedIndex - 1;
// loop on all the plate
for (int PlateIdx = 0; PlateIdx < NumberOfPlates; PlateIdx++)
{
cPlate CurrentPlateToProcess = cGlobalInfo.CurrentScreening.ListPlatesActive.GetPlate(PlateIdx);
for (int IdxValue = 0; IdxValue < cGlobalInfo.CurrentScreening.Columns; IdxValue++)
for (int IdxValue0 = 0; IdxValue0 < cGlobalInfo.CurrentScreening.Rows; IdxValue0++)
{
cWell TmpWell = CurrentPlateToProcess.GetWell(IdxValue, IdxValue0, false);
if (TmpWell == null) continue;
if ((TmpWell.GetCurrentClassIdx() > -1) && (ClassSelectionPanel.GetListSelectedClass()[TmpWell.GetCurrentClassIdx()]))
{
if (DestinatonIdx == -1)
TmpWell.SetAsNoneSelected();
else
TmpWell.SetClass(DestinatonIdx);
Idx++;
}
}
}
cGlobalInfo.CurrentScreening.GetCurrentDisplayPlate().DisplayDistribution(cGlobalInfo.CurrentScreening.ListDescriptors.GetActiveDescriptor(), false);
MessageBox.Show(Idx + " wells have been swapped !", "Process over !", MessageBoxButtons.OK, MessageBoxIcon.Exclamation);
* */
}