public OtsuSegmentation(Panel mainPanel, ImageAnalyser IA)
{
this.IA = IA;
//Core panel
panel.Dock = DockStyle.Top;
panel.BackColor = IA.FileBrowser.BackGround2Color1;
panel.ForeColor = IA.FileBrowser.ShriftColor1;
panel.Visible = false;
panel.Height = 150;
mainPanel.Controls.Add(panel);
panel.BringToFront();
#region Options
GroupBox optionGB = new GroupBox();
optionGB.Text = "Options:";
optionGB.BackColor = IA.FileBrowser.BackGround2Color1;
optionGB.ForeColor = IA.FileBrowser.ShriftColor1;
optionGB.Dock = DockStyle.Top;
optionGB.Height = 85;
panel.Controls.Add(optionGB);
optionGB.BringToFront();
Label Name = new Label();
Name.Text = "Thresholds:";
Name.Width = TextRenderer.MeasureText(Name.Text, Name.Font).Width;
Name.Location = new Point(5, 18);
optionGB.Controls.Add(Name);
Name.BringToFront();
ComboBox cb = thresholdsNumCB;
cb.Text = "0";
cb.Items.AddRange(new string[] { "0", "1", "2", "3", "4" });
cb.Width = 40;
cb.Location = new Point(80, 15);
optionGB.Controls.Add(cb);
cb.BringToFront();
cb.DropDownStyle = ComboBoxStyle.DropDownList;
cb.SelectedIndex = 0;
cb.AutoSize = false;
cb.SelectedIndexChanged += new EventHandler(delegate(object o, EventArgs e)
{
TifFileInfo fi = IA.TabPages.TabCollections[IA.TabPages.SelectedIndex].tifFI;
fi.thresholdsCBoxIndex[fi.cValue] = cb.SelectedIndex;
IA.ReloadImages();
});
CheckBox checkB = sumHistogramsCheckBox;
checkB.Text = "Use SUM histogram";
checkB.Tag = "Use SUM histogram:\nCalculates histograms for all images\nand merge them into one";
checkB.Width = 150;
checkB.Checked = true;
checkB.MouseHover += Control_MouseOver;
checkB.Location = new Point(7, 35);
optionGB.Controls.Add(checkB);
checkB.BringToFront();
checkB.CheckedChanged += new EventHandler(delegate(object o, EventArgs e)
{
TifFileInfo fi = IA.TabPages.TabCollections[IA.TabPages.SelectedIndex].tifFI;
fi.sumHistogramChecked[fi.cValue] = checkB.Checked;
if (((CheckBox)o).Focused == true)
{
IA.ReloadImages();
}
});
{
Button btn = ProcessBtn;
btn.Width = 115;
btn.FlatStyle = FlatStyle.Standard;
btn.BackColor = SystemColors.ButtonFace;
btn.ForeColor = Color.Black;
btn.Text = "Process";
btn.Location = new Point(5, 58);
optionGB.Controls.Add(btn);
btn.BringToFront();
btn.Click += new EventHandler(delegate(object o, EventArgs a)
{
int MLEVEL = thresholdsNumCB.SelectedIndex + 1;
if (IA.Segmentation.SegmentationCBox.SelectedIndex == 2)
{
IA.Segmentation.Kmeans.Start(MLEVEL);
return;
}
if (IA.Segmentation.SegmentationCBox.SelectedIndex != 1)
{
return;
}
TifFileInfo fi = IA.TabPages.TabCollections[IA.TabPages.SelectedIndex].tifFI;
if (fi.available == false)
{
MessageBox.Show("Image is not ready yet! \nTry again later.");
}
else
{
//background worker
var bgw = new BackgroundWorker();
bgw.WorkerReportsProgress = true;
bgw.DoWork += new DoWorkEventHandler(delegate(Object o1, DoWorkEventArgs a1)
{
try
{
//Segmentation event
run(fi, MLEVEL);
}
catch { }
//report progress
((BackgroundWorker)o1).ReportProgress(0);
});
bgw.ProgressChanged += new ProgressChangedEventHandler(delegate(Object o1, ProgressChangedEventArgs a1)
{
fi.available = true;
IA.FileBrowser.StatusLabel.Text = "Ready";
IA.MarkAsNotSaved();
IA.ReloadImages();
});
//Apply status
IA.FileBrowser.StatusLabel.Text = "Segmentation...";
fi.available = false;
//start bgw
bgw.RunWorkerAsync();
}
});
}
#endregion Options
#region Thresholds
GroupBox threshGB = new GroupBox();
threshGB.Text = "Thresholds:";
threshGB.BackColor = IA.FileBrowser.BackGround2Color1;
threshGB.ForeColor = IA.FileBrowser.ShriftColor1;
threshGB.Dock = DockStyle.Fill;
threshGB.Height = 50;
panel.Controls.Add(threshGB);
threshGB.BringToFront();
//Color btns
Panel colorPanel = new Panel();
colorPanel.Dock = DockStyle.Left;
colorPanel.Width = 25;
threshGB.Controls.Add(colorPanel);
Panel UpPanel = new Panel();
UpPanel.Dock = DockStyle.Top;
UpPanel.Height = 15;
threshGB.Controls.Add(UpPanel);
UpPanel.BringToFront();
for (int i = 0; i < colorBtns.Length; i++)
{
Button btn = new Button();
btn.FlatStyle = FlatStyle.Flat;
btn.FlatAppearance.BorderSize = 0;
btn.ForeColor = Color.Black;
btn.Text = "";
btn.Tag = i;
btn.Dock = DockStyle.Top;
btn.Height = 25;
colorPanel.Controls.Add(btn);
colorBtns[i] = btn;
btn.BringToFront();
btn.Visible = false;
btn.MouseDown += new MouseEventHandler(ColorBtn_Click);
btn.MouseHover += new EventHandler(delegate(object o, EventArgs a)
{
if (btn.Text == "")
{
TurnOnToolTip.SetToolTip(btn, "Color " + ((int)btn.Tag).ToString()
+ ":\nLeft click to Disable\nRight click to change color");
}
else
{
TurnOnToolTip.SetToolTip(btn, "Color " + ((int)btn.Tag).ToString()
+ " - Disabled\nLeft click to Enable");
}
});
}
//threshold track bars
for (int i = 0; i < threshTrackBars.Length; i++)
{
CTTrackBar tb = new CTTrackBar();
tb.Initialize();
tb.Panel.Dock = DockStyle.Top;
tb.BackColor(IA.FileBrowser.BackGround2Color1);
tb.ForeColor(IA.FileBrowser.ShriftColor1);
tb.Panel.Visible = false;
tb.Refresh(0, 0, 10);
tb.Name.Text = "T" + (i + 1).ToString();
tb.NamePanel.Width = 30;
threshGB.Controls.Add(tb.Panel);
threshTrackBars[i] = tb;
tb.Panel.BringToFront();
tb.Value.Changed += new ChangedValueEventHandler(delegate(Object o, ChangeValueEventArgs a)
{
TrackBar_ValueChange(a, tb);
});
}
#endregion Thresholds
}
public SpotDetector(Panel mainPanel, ImageAnalyser IA)
{
this.IA = IA;
Panel p1 = new Panel();
p1.Height = 5;
p1.Dock = DockStyle.Top;
mainPanel.Controls.Add(p1);
p1.BringToFront();
#region Threshold
{
CTTrackBar tb = new CTTrackBar();
tb.Initialize();
tb.Panel.Dock = DockStyle.Top;
tb.BackColor(IA.FileBrowser.BackGround2Color1);
tb.ForeColor(IA.FileBrowser.ShriftColor1);
tb.Panel.Visible = true;
tb.Refresh(0, 0, 10);
tb.Name.Text = "Threshold:";
tb.NamePanel.Width = 62;
mainPanel.Controls.Add(tb.Panel);
thresh = tb;
tb.Panel.BringToFront();
tb.Value.Changed += new ChangedValueEventHandler(delegate(Object o, ChangeValueEventArgs a)
{
thresh_valueChange(int.Parse(a.Value));
});
}
#endregion Threshold
#region Options
{
CTTrackBar tb = new CTTrackBar();
tb.Initialize();
tb.Panel.Dock = DockStyle.Top;
tb.BackColor(IA.FileBrowser.BackGround2Color1);
tb.ForeColor(IA.FileBrowser.ShriftColor1);
tb.Panel.Visible = true;
tb.Refresh(100, 1, 100);
tb.Name.Text = "Sensitivity:";
tb.NamePanel.Width = 62;
mainPanel.Controls.Add(tb.Panel);
sensitivity = tb;
tb.Panel.BringToFront();
tb.Value.Changed += new ChangedValueEventHandler(delegate(Object o, ChangeValueEventArgs a)
{
sensitivity_valueChange(int.Parse(a.Value));
});
}
Panel p = new Panel();
p.Height = 30;
p.Dock = DockStyle.Top;
mainPanel.Controls.Add(p);
p.BringToFront();
//Thresh list box
selectT.Tag = "Select Threshold";
selectT.Width = 60;
selectT.Location = new Point(53, 1);
selectT.Items.Add("None");
selectT.DropDownStyle = ComboBoxStyle.DropDownList;
selectT.SelectedIndex = 0;
selectT.AutoSize = false;
p.Controls.Add(selectT);
selectT.BringToFront();
selectT.MouseHover += Control_MouseOver;
selectT.SelectedIndexChanged += selectT_IndexChange;
//ThreshType box
tType.Tag = "Set threshold type";
tType.Width = 60;
tType.Location = new Point(120, 1);
tType.Items.AddRange(new string[] { "Pixels", "% Max" });
tType.DropDownStyle = ComboBoxStyle.DropDownList;
tType.SelectedIndex = 0;
tType.AutoSize = false;
p.Controls.Add(tType);
tType.BringToFront();
tType.MouseHover += Control_MouseOver;
tType.SelectedIndexChanged += TType_IndexChange;
//Color btn
{
Button btn = new Button();
ColBtn = btn;
btn.FlatStyle = FlatStyle.Flat;
btn.FlatAppearance.BorderSize = 0;
btn.BackColor = Color.Red;
btn.ForeColor = Color.Black;
btn.Location = new Point(3, 1);
btn.Text = "";
btn.Tag = "Select color for the spots";
btn.Height = 22;
btn.Width = 25;
p.Controls.Add(btn);
btn.BringToFront();
btn.Visible = true;
btn.MouseHover += Control_MouseOver;
btn.MouseDown += ColBtn_Click;
}
//<>btn
{
Button btn = new Button();
TailType = btn;
btn.FlatStyle = FlatStyle.Flat;
btn.FlatAppearance.BorderSize = 0;
btn.ForeColor = Color.White;
btn.Location = new Point(28, 1);
btn.Text = ">";
btn.Tag = "Set spot intensity diapason";
btn.Height = 22;
btn.Width = 25;
p.Controls.Add(btn);
btn.BringToFront();
btn.Visible = true;
btn.MouseHover += Control_MouseOver;
btn.Click += TailType_Click;
}
#endregion Options
}
private void TrackBar_ValueChange(ChangeValueEventArgs e, CTTrackBar tb)
{
//variables
TifFileInfo fi = IA.TabPages.TabCollections[IA.TabPages.SelectedIndex].tifFI;
int[] vals = fi.thresholdValues[fi.cValue];
int val = int.Parse(e.Value);
int valRef = val;
//find trackbar index
int index = 0;
for (int i = 0; i < threshTrackBars.Length; i++)
{
if (threshTrackBars[i] == tb)
{
index = i; break;
}
}
//Check the avaliable min
if (index > 0)
{
if (val <= vals[index])
{
val = vals[index] + 1;
}
}
//Check the avaliable max
if (index < fi.thresholds[fi.cValue] - 1)
{
if (val >= vals[index + 2])
{
val = vals[index + 2] - 1;
}
}
//refresh if value is wrong
if (val != valRef)
{
tb.Refresh(val, tb.TrackBar1.Minimum, tb.TrackBar1.Maximum);
}
//apply changes
if (val != vals[index + 1])
{
if (tb.TrackBar1.Focused == true | tb.TextBox1.Focused == true |
tb.ApplyBtn.Focused == true)
{
#region apply to history
fi.delHist = true;
IA.delHist = true;
IA.UnDoBtn.Enabled = true;
IA.DeleteFromHistory();
fi.History.Add("segmentation.SetThreshOld("
+ fi.cValue + "," + (index + 1).ToString() + ","
+ vals[index + 1].ToString() + ")");
fi.History.Add("segmentation.SetThreshOld("
+ fi.cValue + "," + (index + 1).ToString() + ","
+ val.ToString() + ")");
IA.UpdateUndoBtns();
IA.MarkAsNotSaved();
#endregion apply to history
}
vals[index + 1] = val;
IA.ReloadImages();
}
}
public void ReloadImage(TifFileInfo fi)
{
#region selectT load
selectT.Items.Clear();
selectT.Items.Add("None");
selectT.Items.Add("Min");
if (IA.Segmentation.SegmentationCBox.SelectedIndex == 1)
{
for (int i = 0; i < fi.thresholds[fi.cValue]; i++)
{
selectT.Items.Add("T" + (i + 1).ToString());
}
}
selectT.Items.Add("Max");
if (fi.SelectedSpotThresh[fi.cValue] < selectT.Items.Count)
{
selectT.SelectedIndex = fi.SelectedSpotThresh[fi.cValue];
}
else
{
selectT.SelectedIndex = 0;
}
#endregion region selectT load
#region type thresh
tType.SelectedIndex = fi.typeSpotThresh[fi.cValue];
switch (tType.SelectedIndex)
{
case 0:
thresh.Refresh(fi.SpotThresh[fi.cValue], 0, Convert.ToInt32(fi.sizeX * fi.sizeY * 0.1));
break;
case 1:
if (fi.SpotThresh[fi.cValue] <= 100)
{
thresh.Refresh(fi.SpotThresh[fi.cValue], 0, 100);
}
else
{
fi.SpotThresh[fi.cValue] = 0;
thresh.Refresh(0, 0, 100);
}
break;
}
#endregion type thresh
Color col = fi.SpotColor[fi.cValue];
if (col != Color.Transparent)
{
ColBtn.BackColor = col;
ColBtn.Text = "";
}
else
{
ColBtn.BackColor = Color.White;
ColBtn.Text = "NaN";
}
TailType.Text = fi.SpotTailType[fi.cValue];
sensitivity.Refresh(fi.spotSensitivity[fi.cValue], 1, 100);
}