public static void RecalculateOriginalROI(ROI original, TifFileInfo newFI, Point[] locs = null)
{
ROI roiOut = original.Duplicate();
int max = roiOut.Type == 0 ? 1 : newFI.imageCount;
for (int i = 0; i < max; i += newFI.sizeC)
{
var points = roiOut.GetLocation(i + newFI.cValue);
Point newP = locs == null? new Point(newFI.xCompensation, newFI.yCompensation):locs[i];
Point p;
for (int ind = 0; ind < points.Length; ind++)
{
p = points[ind];
p.X = p.X - newP.X;
p.Y = p.Y - newP.Y;
points[ind] = p;
}
roiOut.SetLocation(i, points);
}
newFI.roiList = new List <ROI> [newFI.sizeC];
for (int c = 0; c < newFI.sizeC; c++)
{
newFI.roiList[c] = new List <ROI>();
}
newFI.roiList[0].Add(roiOut);
if (newFI.sizeC > 1)
{
for (int c = 1; c < newFI.sizeC; c++)
{
ROI newROI = roiOut.Duplicate();
for (int i = 0; i < newFI.imageCount; i += newFI.sizeC)
{
var points = roiOut.GetLocation(i);
newROI.SetLocation(i + c, points);
}
newFI.roiList[c].Add(newROI);
}
}
}
public static void RecalculateOriginalROI(ROI original, ROI modified, TifFileInfo newFI)
{
if (modified != null)
{
for (int i = 0; i < newFI.imageCount; i += newFI.sizeC)
{
var points = original.GetLocation(i);
Point newP = modified.GetLocation(i)[0];
Point p;
for (int ind = 0; ind < points.Length; ind++)
{
p = points[ind];
p.X = p.X - newP.X;
p.Y = p.Y - newP.Y;
points[ind] = p;
}
original.SetLocation(i, points);
}
}
newFI.roiList = new List <ROI> [newFI.sizeC];
for (int c = 0; c < newFI.sizeC; c++)
{
newFI.roiList[c] = new List <ROI>();
}
newFI.roiList[0].Add(original);
if (newFI.sizeC > 1)
{
for (int c = 1; c < newFI.sizeC; c++)
{
ROI newROI = original.Duplicate();
for (int i = 0; i < newFI.imageCount; i += newFI.sizeC)
{
var points = original.GetLocation(i);
newROI.SetLocation(i + c, points);
}
newFI.roiList[c].Add(newROI);
}
}
}
public static List <Point> GetROIPoints(int frame, ROI roi, TifFileInfo fi)
{
switch (roi.Shape)
{
case 0:
return(GetPointsInRectangleTracking(roi, fi, frame));
case 1:
return(GetPointsInOvalTracking(roi, fi, frame));
default:
return(GetPolygonPoints(roi.GetLocation(frame), fi));
}
}
private static List <Point> GetPointsInOvalTracking(ROI roi, TifFileInfo fi, int frame)
{
//FillEllipse(roi, fi, 0);
//get the location of the first value
Point p = roi.GetLocation(frame)[0];
int X = p.X;
int Y = p.Y;
int W = roi.Width;
int H = roi.Height;
//create shablon for preventing retacking the same value
bool[,] shablon = new bool[fi.sizeY, fi.sizeX];
return(CalculateEllipse(shablon, X, Y, W, H));
}
private static List <Point> GetPointsInRectangleTracking(ROI roi, TifFileInfo fi, int frame)
{
//get the location of the first value
Point p = roi.GetLocation(frame)[0];
int X = p.X;
int Y = p.Y;
int W = roi.Width;
int H = roi.Height;
//create shablon for preventing retacking the same value
bool[,] shablon = new bool[fi.sizeY, fi.sizeX];
X -= roi.D * roi.Stack;
Y -= roi.D * roi.Stack;
W += (roi.D + roi.D) * roi.Stack;
H += (roi.D + roi.D) * roi.Stack;
return(CalculateRectangle(shablon, X, Y, W, H));
}
private void CropToolStripMenuItem_click(ROI roi, string dir)
{
if (checkBox_Tracking.Checked && roi.Type == 1)
{
TrackCropToolStripMenuItem_click(roi, dir);
return;
}
fi.available = false;
TifFileInfo newFI = null;
Rectangle rect = Rectangle.Empty;
//find rectangles
switch (roi.Type)
{
case 0:
if (roi.Shape == 1 | roi.Shape == 0)
{
Point p = roi.GetLocation(fi.cValue)[0];
Size size = new Size(roi.Width, roi.Height);
rect = new Rectangle(p, size);
}
else if (roi.Shape == 2 || roi.Shape == 3 || roi.Shape == 4 || roi.Shape == 5)
{
Point[] pList = roi.GetLocation(fi.cValue);
int X = int.MaxValue;
int Y = int.MaxValue;
int W = int.MinValue;
int H = int.MinValue;
foreach (Point p1 in pList)
{
if (p1.X < X)
{
X = p1.X;
}
if (p1.Y < Y)
{
Y = p1.Y;
}
if (p1.X > W)
{
W = p1.X;
}
if (p1.Y > H)
{
H = p1.Y;
}
}
Point p = new Point(X, Y);
Size size = new Size(W - X, H - Y);
rect = new Rectangle(p, size);
}
break;
case 1:
if (roi.Shape == 1 | roi.Shape == 0)
{
Point[] pList = roi.GetLocationAll()[0];
int X = int.MaxValue;
int Y = int.MaxValue;
int W = int.MinValue;
int H = int.MinValue;
for (int i = fi.cValue; i < fi.imageCount; i += fi.sizeC)
{
Point p1 = pList[i];
if (p1 != null)
{
if (p1.X < X)
{
X = p1.X;
}
if (p1.Y < Y)
{
Y = p1.Y;
}
if (p1.X > W)
{
W = p1.X;
}
if (p1.Y > H)
{
H = p1.Y;
}
}
}
Point p = new Point(X, Y);
Size size = new Size(W - X + roi.Width, H - Y + roi.Height);
rect = new Rectangle(p, size);
}
else if (roi.Shape == 2 || roi.Shape == 3 || roi.Shape == 4 || roi.Shape == 5)
{
int X = int.MaxValue;
int Y = int.MaxValue;
int W = int.MinValue;
int H = int.MinValue;
Point[] pList;
for (int i = fi.cValue; i < fi.imageCount; i += fi.sizeC)
{
pList = roi.GetLocation(i);
foreach (Point p1 in pList)
{
if (p1.X < X)
{
X = p1.X;
}
if (p1.Y < Y)
{
Y = p1.Y;
}
if (p1.X > W)
{
W = p1.X;
}
if (p1.Y > H)
{
H = p1.Y;
}
}
}
Point p = new Point(X, Y);
Size size = new Size(W - X, H - Y);
rect = new Rectangle(p, size);
}
break;
}
//crop the rectangle
newFI = DuplicateFI(fi);
newFI.Dir = newFI.Dir.Substring(0, newFI.Dir.LastIndexOf(".")) + "_ROI"
+ (fi.roiList[fi.cValue].IndexOf(roi) + 1).ToString() + ".tif";
newFI.sizeX = rect.Width;
newFI.sizeY = rect.Height;
newFI.xCompensation = rect.X;
newFI.yCompensation = rect.Y;
newFI.imageCount = fi.imageCount;
newFI.openedImages = newFI.imageCount;
AddEmptyArraysToFI(newFI);
switch (fi.bitsPerPixel)
{
case 8:
byte[][][] image8bit = new byte[fi.imageCount][][];
Parallel.For(0, fi.imageCount, frame =>
{
image8bit[frame] = new byte[rect.Height][];
for (int y = rect.Y, yNew = 0; y < rect.Y + rect.Height; y++, yNew++)
{
image8bit[frame][yNew] = new byte[rect.Width];
for (int x = rect.X, xNew = 0; x < rect.X + rect.Width; x++, xNew++)
{
if (x >= 0 && y >= 0 && x < fi.sizeX && y < fi.sizeY)
{
image8bit[frame][yNew][xNew] = fi.image8bit[frame][y][x];
}
}
}
});
newFI.image8bit = image8bit;
newFI.image8bitFilter = newFI.image8bit;
break;
case 16:
ushort[][][] image16bit = new ushort[fi.imageCount][][];
Parallel.For(0, fi.imageCount, frame =>
{
image16bit[frame] = new ushort[rect.Height][];
for (int y = rect.Y, yNew = 0; y < rect.Y + rect.Height; y++, yNew++)
{
image16bit[frame][yNew] = new ushort[rect.Width];
for (int x = rect.X, xNew = 0; x < rect.X + rect.Width; x++, xNew++)
{
if (x >= 0 && y >= 0 && x < fi.sizeX && y < fi.sizeY)
{
image16bit[frame][yNew][xNew] = fi.image16bit[frame][y][x];
}
}
}
});
newFI.image16bit = image16bit;
newFI.image16bitFilter = newFI.image16bit;
break;
}
newFI.loaded = true;
newFI.original = false;
if (CropRoi)
{
ROI roi1 = roi.Duplicate();
RecalculateOriginalROI(roi1, newFI);
}
fi.available = true;
FileEncoder.SaveTif(newFI, dir);
}