public void Configure(ImagingParameters iParams, ProjectContainer project, PlateContainer plate, ExperimentContainer experiment, MethodContainer method, MaskContainer mask, PlateTypeContainer plateType, ObservableCollection <ExperimentIndicatorContainer> indicatorList, ObservableCollection <ExperimentCompoundPlateContainer> compoundPlateList, ObservableCollection <Tuple <int, int> > controlSubtractionWellList, int numFoFrames, ExperimentIndicatorContainer dynamicRatioNumerator, ExperimentIndicatorContainer dynamicRatioDenominator) { m_iParams = iParams; m_project = project; m_experimentPlate = plate; m_experiment = experiment; m_method = method; m_mask = mask; m_plateType = plateType; m_indicatorList = indicatorList; m_compoundPlateList = compoundPlateList; m_controlSubtractionWellList = controlSubtractionWellList; m_numFoFrames = numFoFrames; m_dynamicRatioNumerator = dynamicRatioNumerator; m_dynamicRatioDenominator = dynamicRatioDenominator; m_vworksProtocolFilename = m_method.BravoMethodFile; ChartArray.BuildChartArray(mask.Rows, mask.Cols, m_iParams.HorzBinning, m_iParams.VertBinning, m_indicatorList); Dictionary <int, ImageDisplay> idDictionary = ChartArray.GetImageDisplayDictionary(); m_displayPipeline = m_imager.CreateDisplayPipeline(m_uiTask, idDictionary); m_storagePipeline = m_imager.CreateImageStoragePipeline(GlobalVars.CompressionAlgorithm, m_iParams.imageWidth, m_iParams.imageHeight); int numerID = 0; int denomID = 0; if (m_dynamicRatioNumerator != null) { numerID = m_dynamicRatioNumerator.ExperimentIndicatorID; } if (m_dynamicRatioDenominator != null) { denomID = m_dynamicRatioDenominator.ExperimentIndicatorID; } m_analysisPipeline = m_imager.CreateAnalysisPipeline(ChartArray, m_mask, m_iParams.imageWidth, m_iParams.imageHeight, m_iParams.HorzBinning, m_iParams.VertBinning, m_iParams.ExperimentIndicatorID, m_controlSubtractionWellList, m_numFoFrames, numerID, denomID); if (m_histogram != null) { m_histogramPipeline = m_imager.CreateHistogramPipeline(m_uiTask, m_histogram); } }
public async void StartVideo(int numberOfImagesToTake) { if (m_imager == null) { return; } VM.StatusString = "Imaging"; if (numberOfImagesToTake == 1) { SnapShotPB.Content = "Cancel"; VideoPB.IsEnabled = false; } else { SnapShotPB.IsEnabled = false; VideoPB.Content = "Cancel"; } GainSpin.IsEnabled = false; ExposureSpin.IsEnabled = false; Optimize1PB.IsEnabled = false; //ExcitationFilterCombo.IsEnabled = false; //EmissionFilterCombo.IsEnabled = false; m_uiTask = TaskScheduler.FromCurrentSynchronizationContext(); m_iParams = new ImagingParameters(); m_iParams.NumIndicators = 1; m_iParams.NumImages = numberOfImagesToTake; m_iParams.Exposure[0] = ((float)VM.Exposure) / 1000; m_iParams.CycleTime[0] = VM.Exposure + 250; m_iParams.ExcitationFilter[0] = (byte)VM.ExFilter.PositionNumber; m_iParams.EmissionFilter[0] = (byte)VM.EmFilter.PositionNumber; m_iParams.ExcitationFilterChangeSpeed = 5; m_iParams.EmissionFilterChangeSpeed = 5; m_iParams.Gain[0] = VM.Gain; m_iParams.HorzBinning = VM.HorzBinning; m_iParams.VertBinning = VM.VertBinning; m_iParams.LampShutterIsOpen[0] = true; m_iParams.LightIntensity = 100; m_iParams.SyncExcitationFilterWithImaging = true; m_iParams.Image_StartCol = VM.RoiX; m_iParams.Image_EndCol = VM.RoiX + VM.RoiW - 1; m_iParams.Image_StartRow = VM.RoiY; m_iParams.Image_EndRow = VM.RoiY + VM.RoiH - 1; m_iParams.imageWidth = VM.RoiW; m_iParams.imageHeight = VM.RoiH; m_iParams.maxPixelValue = (2 ^ m_imager.m_camera.BitDepth) - 1; m_iParams.ExperimentIndicatorID[0] = 0; // 0 here indicates that images will not be saved m_iParams.IndicatorName[0] = VM.IndicatorName; m_tokenSource = new CancellationTokenSource(); m_cancelToken = m_tokenSource.Token; Progress <int> progress = new Progress <int>(); progress.ProgressChanged += (sender1, num) => { VM.StatusString = "Completed " + num.ToString() + " images"; }; ImageDisplayControl.SetImageSize(m_iParams.imageWidth, m_iParams.imageHeight, m_iParams.maxPixelValue); m_bitmap = ImageDisplayControl.m_imageBitmap; Task ImagingTask = Task.Factory.StartNew(() => m_imager.StartImaging(m_iParams, null, // no analysis parameters needed here m_cancelToken, m_uiTask, progress, ImageDisplayControl, m_colorModel.m_colorMap, m_histogram), m_cancelToken); try { await ImagingTask; } catch (AggregateException aggEx) { StringBuilder sb = new StringBuilder(); sb.Append("Exception(s) occurred: "); foreach (Exception ex in aggEx.InnerExceptions) { sb.Append(ex.Message); sb.Append(", "); } MessageBoxResult result = MessageBox.Show(sb.ToString(), "Exception(s) Occurred", MessageBoxButton.OK, MessageBoxImage.Error); } catch (OperationCanceledException) { VM.StatusString = "Canceled"; } catch (Exception ex) { MessageBoxResult result = MessageBox.Show(ex.Message, "Unknown Exception Occurred", MessageBoxButton.OK, MessageBoxImage.Error); } finally { ImagingTask.Dispose(); OnImagingStopped(EventArgs.Empty); VideoPB.Content = "Video"; SnapShotPB.Content = "Snap Shot"; VideoPB.IsEnabled = true; SnapShotPB.IsEnabled = true; GainSpin.IsEnabled = true; ExposureSpin.IsEnabled = true; Optimize1PB.IsEnabled = true; //ExcitationFilterCombo.IsEnabled = true; //EmissionFilterCombo.IsEnabled = true; VM.IsImaging = false; VM.StatusString = "Ready"; } }
void LoadSimulationConfiguration() { // m_iParams = iParams; // m_project = project; // m_experimentPlate = plate; // m_method = method; // m_mask = mask; // m_plateType = plateType; // m_indicatorList = indicatorList; // m_compoundPlateList = compoundPlateList; // m_controlSubtractionWellList = controlSubtractionWellList; //m_numFoFrames = numFoFrames; //m_dynamicRatioNumerator = dynamicRatioNumerator; //m_dynamicRatioDenominator = dynamicRatioDenominator; //ChartArray.BuildChartArray(mask.Rows, mask.Cols, m_indicatorList); //////////////////////// // set up project m_project = null; bool success = m_wgDB.GetAllProjects(false); foreach (ProjectContainer project in m_wgDB.m_projectList) { if (project.Description.Equals("Debug", StringComparison.OrdinalIgnoreCase)) { m_project = project; break; } } if (m_project == null) // not found in database, so create it { m_project = new ProjectContainer(); m_project.Description = "Debug"; m_project.TimeStamp = DateTime.Now; m_project.Archived = false; success = m_wgDB.InsertProject(ref m_project); } //////////////////////// // set up plateType m_plateType = null; success = m_wgDB.GetAllPlateTypes(); if (m_wgDB.m_plateTypeList.Count() > 0) { m_plateType = m_wgDB.m_plateTypeList.ElementAt(0); } else { // create a new plateType m_plateType = new PlateTypeContainer(); m_plateType.Cols = 24; m_plateType.Description = "Debug"; m_plateType.IsDefault = false; m_plateType.Rows = 16; success = m_wgDB.InsertPlateType(ref m_plateType); } //////////////////////// // set up experiment plate m_experimentPlate = null; success = m_wgDB.GetAllPlatesForProject(m_project.ProjectID); if (m_wgDB.m_plateList.Count() > 0) { m_experimentPlate = m_wgDB.m_plateList.ElementAt(0); } else { // create a new plate m_experimentPlate = new PlateContainer(); m_experimentPlate.Barcode = "12345678"; m_experimentPlate.Description = "Debug"; m_experimentPlate.IsPublic = true; m_experimentPlate.OwnerID = GlobalVars.UserID; m_experimentPlate.PlateTypeID = m_plateType.PlateTypeID; m_experimentPlate.ProjectID = m_project.ProjectID; success = m_wgDB.InsertPlate(ref m_experimentPlate); } //////////////////////// // set up method m_method = null; success = m_wgDB.GetAllMethodsForUser(GlobalVars.UserID); foreach (MethodContainer method in m_wgDB.m_methodList) { if (method.Description.Equals("Debug", StringComparison.OrdinalIgnoreCase)) { m_method = method; break; } } if (m_method == null) { m_method = new MethodContainer(); m_method.BravoMethodFile = ""; m_method.Description = "Debug"; m_method.IsPublic = true; m_method.OwnerID = GlobalVars.UserID; success = m_wgDB.InsertMethod(ref m_method); } success = m_wgDB.GetAllIndicatorsForMethod(m_method.MethodID); if (m_wgDB.m_indicatorList.Count < 1) { // create indicators for this new method IndicatorContainer ind = new IndicatorContainer(); ind.Description = "Debug"; ind.EmissionsFilterPosition = 6; ind.ExcitationFilterPosition = 4; ind.MethodID = m_method.MethodID; ind.SignalType = SIGNAL_TYPE.UP; success = m_wgDB.InsertIndicator(ref ind); } //////////////////////// // set up experiment m_experiment = null; ObservableCollection <ExperimentContainer> expList; success = m_wgDB.GetAllExperimentsForPlate(m_experimentPlate.PlateID, out expList); if (expList.Count() > 0) { m_experiment = expList.ElementAt(0); } else { m_experiment = new ExperimentContainer(); m_experiment.Description = "Debug"; m_experiment.HorzBinning = 1; m_experiment.MethodID = m_method.MethodID; m_experiment.PlateID = m_experimentPlate.PlateID; m_experiment.ROI_Height = GlobalVars.PixelHeight; m_experiment.ROI_Width = GlobalVars.PixelWidth; m_experiment.ROI_Origin_X = 1; m_experiment.ROI_Origin_Y = 1; m_experiment.TimeStamp = DateTime.Now; m_experiment.VertBinning = 1; success = m_wgDB.InsertExperiment(ref m_experiment); } //////////////////////// // set up mask m_mask = null; success = m_wgDB.GetAllMasksForPlateType(m_experimentPlate.PlateTypeID); if (m_wgDB.m_maskList.Count() > 0) { m_mask = m_wgDB.m_maskList.ElementAt(0); } else { // create a new mask m_mask = new MaskContainer(); m_mask.Angle = 0.0; m_mask.Cols = 24; m_mask.Description = "Debug"; m_mask.IsDefault = false; m_mask.NumEllipseVertices = 24; m_mask.PlateTypeID = m_experimentPlate.PlateTypeID; m_mask.ReferenceImageID = 0; m_mask.Rows = 16; m_mask.Shape = 0; m_mask.XOffset = 28; m_mask.XSize = 28; m_mask.XStep = 41.522; m_mask.YOffset = 190; m_mask.YSize = 28; m_mask.YStep = 41.467; success = m_wgDB.InsertMask(ref m_mask); } //////////////////////////////////// // setup test indicator(s) m_indicatorList = new ObservableCollection <ExperimentIndicatorContainer>(); ObservableCollection <ExperimentIndicatorContainer> expIndList; success = m_wgDB.GetAllExperimentIndicatorsForExperiment(m_experiment.ExperimentID, out expIndList); if (expIndList.Count > 0) { foreach (ExperimentIndicatorContainer ex in expIndList) { m_indicatorList.Add(ex); } } else { success = m_wgDB.GetAllIndicatorsForMethod(m_method.MethodID); foreach (IndicatorContainer ind in m_wgDB.m_indicatorList) { ExperimentIndicatorContainer exInd = new ExperimentIndicatorContainer(); exInd.Description = ind.Description; FilterContainer filter; success = m_wgDB.GetExcitationFilterAtPosition(ind.ExcitationFilterPosition, out filter); exInd.ExcitationFilterDesc = filter.Description; success = m_wgDB.GetEmissionFilterAtPosition(ind.EmissionsFilterPosition, out filter); exInd.EmissionFilterDesc = filter.Description; exInd.EmissionFilterPos = ind.EmissionsFilterPosition; exInd.ExcitationFilterPos = ind.ExcitationFilterPosition; exInd.ExperimentID = m_experiment.ExperimentID; exInd.Exposure = 150; exInd.Gain = 5; exInd.MaskID = m_mask.MaskID; exInd.SignalType = SIGNAL_TYPE.UP; exInd.Verified = true; success = m_wgDB.InsertExperimentIndicator(ref exInd); m_indicatorList.Add(exInd); } } //////////////////////////////////// // compound plates m_compoundPlateList = new ObservableCollection <ExperimentCompoundPlateContainer>(); //////////////////////////////////// // control subtraction well list m_controlSubtractionWellList = new ObservableCollection <Tuple <int, int> >(); // all wells in last row = control wells for (int c = 0; c < m_mask.Cols; c++) { m_controlSubtractionWellList.Add(Tuple.Create(m_mask.Rows - 1, c)); } //////////////////////////////////// //m_numFoFrames = numFoFrames; m_numFoFrames = 5; if (m_indicatorList.Count() > 1) { //////////////////////////////////// //m_dynamicRatioNumerator = dynamicRatioNumerator; m_dynamicRatioNumerator = m_indicatorList.ElementAt(0); //////////////////////////////////// //m_dynamicRatioDenominator = dynamicRatioDenominator; m_dynamicRatioDenominator = m_indicatorList.ElementAt(1); } else { m_dynamicRatioNumerator = new ExperimentIndicatorContainer(); m_dynamicRatioNumerator.ExperimentIndicatorID = 0; m_dynamicRatioDenominator = new ExperimentIndicatorContainer(); m_dynamicRatioDenominator.ExperimentIndicatorID = 0; } if (m_iParams == null) { m_iParams = new ImagingParameters(); } m_iParams.maxPixelValue = GlobalVars.MaxPixelValue; m_iParams.imageWidth = GlobalVars.PixelWidth; m_iParams.imageHeight = GlobalVars.PixelHeight; m_iParams.Image_StartCol = 1; m_iParams.Image_EndCol = GlobalVars.PixelWidth; m_iParams.Image_StartRow = 1; m_iParams.Image_EndRow = GlobalVars.PixelHeight; m_iParams.BravoMethodFilename = ""; m_iParams.CameraTemperature = GlobalVars.CameraTargetTemperature; m_iParams.HorzBinning = 1; m_iParams.VertBinning = 1; m_iParams.EmissionFilterChangeSpeed = GlobalVars.FilterChangeSpeed; m_iParams.ExcitationFilterChangeSpeed = GlobalVars.FilterChangeSpeed; m_iParams.LightIntensity = 100; m_iParams.NumImages = 1000000; // artificial limit on number of images m_iParams.NumIndicators = m_indicatorList.Count; m_iParams.SyncExcitationFilterWithImaging = true; m_iParams.CycleTime = new int[m_indicatorList.Count]; m_iParams.EmissionFilter = new byte[m_indicatorList.Count]; m_iParams.ExcitationFilter = new byte[m_indicatorList.Count]; m_iParams.Exposure = new float[m_indicatorList.Count]; m_iParams.Gain = new int[m_indicatorList.Count]; m_iParams.ExperimentIndicatorID = new int[m_indicatorList.Count]; m_iParams.IndicatorName = new string[m_indicatorList.Count]; m_iParams.LampShutterIsOpen = new bool[m_indicatorList.Count]; int i = 0; foreach (ExperimentIndicatorContainer ind in m_indicatorList) { m_iParams.CycleTime[i] = 1000; m_iParams.EmissionFilter[i] = (byte)ind.EmissionFilterPos; m_iParams.ExcitationFilter[i] = (byte)ind.ExcitationFilterPos; m_iParams.Exposure[i] = (float)ind.Exposure / 1000; m_iParams.Gain[i] = ind.Gain; m_iParams.ExperimentIndicatorID[i] = 0; // created by the RunExperiment object when the experiment is run m_iParams.IndicatorName[i] = ind.Description; m_iParams.LampShutterIsOpen[i] = true; m_iParams.ExperimentIndicatorID[i] = ind.ExperimentIndicatorID; i++; } }
////////////////////////////////////////////////////////////////////////////////////////// ////////////////////////////////////////////////////////////////////////////////////////// ////////////////////////////////////////////////////////////////////////////////////////// public async void StartVideo(ImagingParameters iParams) { if (m_imager == null) { return; } //m_uiTask = TaskScheduler.FromCurrentSynchronizationContext(); m_tokenSource = new CancellationTokenSource(); m_cancelToken = m_tokenSource.Token; Progress <int> progress = new Progress <int>(); progress.ProgressChanged += (sender1, num) => { VM.MessageText = num.ToString() + " images"; }; Dictionary <int, ImageDisplay> imageDisplayDictionary = ChartArray.GetImageDisplayDictionary(); foreach (KeyValuePair <int, ImageDisplay> entry in imageDisplayDictionary) { ImageDisplay imageDisplay = entry.Value; imageDisplay.SetImageSize(iParams.imageWidth, m_iParams.imageHeight, m_iParams.maxPixelValue); } Task ImagingTask = Task.Factory.StartNew(() => m_imager.StartImaging(m_iParams, Tuple.Create(m_mask, m_controlSubtractionWellList, m_numFoFrames, m_dynamicRatioNumerator.ExperimentIndicatorID, m_dynamicRatioDenominator.ExperimentIndicatorID), m_cancelToken, m_uiTask, progress, null, m_colorModel.m_colorMap, m_histogram, ChartArray), m_cancelToken); try { await ImagingTask; } catch (AggregateException aggEx) { StringBuilder sb = new StringBuilder(); sb.Append("Exception(s) occurred: "); foreach (Exception ex in aggEx.InnerExceptions) { sb.Append(ex.Message); sb.Append(", "); } MessageBoxResult result = MessageBox.Show(sb.ToString(), "Exception(s) Occurred", MessageBoxButton.OK, MessageBoxImage.Error); } catch (OperationCanceledException) { } catch (Exception ex) { MessageBoxResult result = MessageBox.Show(ex.Message, "Unknown Exception Occurred", MessageBoxButton.OK, MessageBoxImage.Error); } finally { ImagingTask.Dispose(); } }