// Write all the header sections that come before the features section.
private void WriteHeaders(ISequence sequence, TextWriter writer)
{
GenBankMetadata metadata = (GenBankMetadata)sequence.Metadata[Helper.GenBankMetadataKey];
if (metadata != null)
{
WriteLocus(sequence, writer);
WriteHeaderSection("DEFINITION", metadata.Definition, writer);
if (metadata.Accession != null)
{
WriteHeaderSection("ACCESSION", Helper.GetGenBankAccession(metadata.Accession), writer);
string version = "";
if (metadata.Version != null)
{
version = metadata.Accession.Primary + "." + metadata.Version.Version;
if (!string.IsNullOrEmpty(metadata.Version.GINumber))
{
version += " GI:" + metadata.Version.GINumber;
}
if (version.Length > 0)
{
WriteHeaderSection("VERSION", version, writer);
}
}
}
if (metadata.Project != null)
{
WriteHeaderSection("PROJECT", Helper.GetProjectIdentifier(metadata.Project), writer);
}
if (metadata.DBLink != null)
{
WriteHeaderSection("DBLINK", Helper.GetCrossReferenceLink(metadata.DBLink), writer);
}
WriteHeaderSection("DBSOURCE", metadata.DBSource, writer);
WriteHeaderSection("KEYWORDS", metadata.Keywords, writer);
if (metadata.Segment != null)
{
WriteHeaderSection("SEGMENT", Helper.GetSequenceSegment(metadata.Segment), writer);
}
WriteSource(metadata, writer);
WriteReferences(metadata, writer);
WriteComments(metadata, writer);
WriteHeaderSection("PRIMARY", metadata.Primary, writer);
}
}
// Handle optional BASE COUNT, then ORIGIN and sequence data.
private void ParseSequence(MBFTextReader mbfReader, ref Sequence sequence)
{
string message = string.Empty;
GenBankMetadata metadata = (GenBankMetadata)sequence.Metadata[Helper.GenBankMetadataKey];
// set data indent for sequence headers
mbfReader.DataIndent = _dataIndent;
while (mbfReader.HasLines)
{
if (mbfReader.Line.StartsWith("//", StringComparison.Ordinal))
{
mbfReader.GoToNextLine();
break; // end of sequence record
}
switch (mbfReader.LineHeader)
{
case "BASE COUNT":
// The BASE COUNT linetype is obsolete and was removed
// from the GenBank flatfile format in October 2003. But if it is
// present, we will use it. We get the untrimmed version since it
// starts with a right justified column.
metadata.BaseCount = mbfReader.Line.Substring(_dataIndent);
mbfReader.GoToNextLine();
break;
case "ORIGIN":
// Change Note: The original implementation would validate the alphabet every line
// which would greatly impact performance on large sequences. This updates the method
// to improve performance by validating the alphabet after parsing the sequence.
ParseOrigin(mbfReader, metadata, ref sequence);
break;
case "CONTIG":
metadata.Contig = ParseMultiLineData(mbfReader, Environment.NewLine);
// don't go to next line; current line still needs to be processed
break;
default:
message = String.Format(
CultureInfo.CurrentCulture,
Properties.Resource.ParserUnexpectedLineInSequence,
mbfReader.Line);
Trace.Report(message);
throw new InvalidDataException(message);
}
}
}
private void WriteFeatures(ISequence sequence, TextWriter writer)
{
ILocationBuilder locBuilder = LocationBuilder;
if (locBuilder == null)
{
throw new InvalidOperationException(Resource.NullLocationBuild);
}
GenBankMetadata metadata = (GenBankMetadata)sequence.Metadata[Helper.GenBankMetadataKey];
if (metadata != null && metadata.Features != null)
{
WriteFeatureSection("FEATURES", "Location/Qualifiers", writer);
// write the features in the order they were put in the list
foreach (FeatureItem feature in metadata.Features.All)
{
WriteFeatureSection(_featureHeaderIndentString + feature.Key, locBuilder.GetLocationString(feature.Location), writer);
// The sub-items of a feature are referred to as qualifiers. These do not have
// unique keys, so they are stored as lists in the SubItems dictionary.
foreach (KeyValuePair <string, List <string> > qualifierList in feature.Qualifiers)
{
foreach (string qualifierValue in qualifierList.Value)
{
string data = "/" + qualifierList.Key;
if (qualifierValue != string.Empty)
{
data += "=" + qualifierValue;
}
// use a blank header; the qualifier key is part of the data
WriteFeatureSection(string.Empty, data, writer);
}
}
}
}
}
private void WriteReferences(GenBankMetadata metadata, TextWriter writer)
{
if (metadata.References != null)
{
foreach (CitationReference reference in metadata.References)
{
// format the data for the first line
string data = reference.Number.ToString();
if (!string.IsNullOrEmpty(reference.Location))
{
data = data.PadRight(3) + "(" + reference.Location + ")";
}
WriteHeaderSection("REFERENCE", data, writer);
WriteHeaderSection(" AUTHORS", reference.Authors, writer);
WriteHeaderSection(" CONSRTM", reference.Consortiums, writer);
WriteHeaderSection(" TITLE", reference.Title, writer);
WriteHeaderSection(" JOURNAL", reference.Journal, writer);
WriteHeaderSection(" MEDLINE", reference.Medline, writer);
WriteHeaderSection(" PUBMED", reference.PubMed, writer);
WriteHeaderSection(" REMARK", reference.Remarks, writer);
}
}
}
private static void ParseSource(MBFTextReader mbfReader, ref Sequence sequence)
{
string source = string.Empty;
string organism = string.Empty;
string classLevels = string.Empty;
while (mbfReader.HasLines)
{
if (mbfReader.LineHeader == "SOURCE")
{
// data can be multiline. spec says last line must end with period
// (note: this doesn't apply unless multiline)
bool lastDotted = true;
source = mbfReader.LineData;
mbfReader.GoToNextLine();
while (mbfReader.HasLines && !mbfReader.LineHasHeader)
{
source += " " + mbfReader.LineData;
lastDotted = (source.EndsWith(".", StringComparison.Ordinal));
mbfReader.GoToNextLine();
}
if (!lastDotted && Trace.Want(Trace.SeqWarnings))
{
Trace.Report("GenBank.ParseSource", Properties.Resource.OutOfSpec, source);
}
// don't go to next line; current line still needs to be processed
}
else if (mbfReader.Line[0] == ' ')
{
if (mbfReader.LineHeader != "ORGANISM")
{
string message = String.Format(
CultureInfo.CurrentCulture,
Properties.Resource.ParserInvalidSourceField,
mbfReader.LineHeader);
Trace.Report(message);
throw new InvalidDataException(message);
}
// this also can be multiline
organism = mbfReader.LineData;
mbfReader.GoToNextLine();
while (mbfReader.HasLines && !mbfReader.LineHasHeader)
{
if (mbfReader.Line.EndsWith(";", StringComparison.Ordinal) || mbfReader.Line.EndsWith(".", StringComparison.Ordinal))
{
if (!String.IsNullOrEmpty(classLevels))
{
classLevels += " ";
}
classLevels += mbfReader.LineData;
}
else
{
organism += " " + mbfReader.LineData;
}
mbfReader.GoToNextLine();
}
// don't go to next line; current line still needs to be processed
}
else
{
// don't go to next line; current line still needs to be processed
break;
}
}
GenBankMetadata metadata = (GenBankMetadata)sequence.Metadata[Helper.GenBankMetadataKey];
metadata.Source = new SequenceSource();
metadata.Source.CommonName = source;
if (!string.IsNullOrEmpty(organism))
{
int index = organism.IndexOf(" ", StringComparison.Ordinal);
if (index > 0)
{
metadata.Source.Organism.Genus = organism.Substring(0, index);
if (organism.Length > index)
{
index++;
metadata.Source.Organism.Species = organism.Substring(index, organism.Length - index);
}
}
else
{
metadata.Source.Organism.Genus = organism;
}
}
metadata.Source.Organism.ClassLevels = classLevels;
}
private static void ParseReferences(MBFTextReader mbfReader, ref Sequence sequence)
{
GenBankMetadata metadata = (GenBankMetadata)sequence.Metadata[Helper.GenBankMetadataKey];
IList <CitationReference> referenceList = metadata.References;
CitationReference reference = null;
//List<MetadataListItem<string>> referenceList = new List<MetadataListItem<string>>();
//MetadataListItem<string> reference = null;
while (mbfReader.HasLines)
{
if (mbfReader.LineHeader == "REFERENCE")
{
// add previous reference
if (reference != null)
{
referenceList.Add(reference);
}
// check for start/end e.g. (bases 1 to 118), or prose notes
Match m = Regex.Match(mbfReader.LineData,
@"^(?<number>\d+)(\s+\((?<location>.*)\))?");
if (!m.Success)
{
string message = String.Format(
CultureInfo.CurrentCulture,
Properties.Resource.ParserReferenceError,
mbfReader.LineData);
Trace.Report(message);
throw new InvalidDataException(message);
}
// create new reference
string number = m.Groups["number"].Value;
string location = m.Groups["location"].Value;
reference = new CitationReference();
int outValue;
if (!int.TryParse(number, out outValue))
{
throw new InvalidOperationException();
}
reference.Number = outValue;
reference.Location = location;
mbfReader.GoToNextLine();
}
else if (mbfReader.Line.StartsWith(" ", StringComparison.Ordinal))
{
switch (mbfReader.LineHeader)
{
// all the following are extracted the same way - possibly multiline
case "AUTHORS":
reference.Authors = ParseMultiLineData(mbfReader, " ");
break;
case "CONSRTM":
reference.Consortiums = ParseMultiLineData(mbfReader, " ");
break;
case "TITLE":
reference.Title = ParseMultiLineData(mbfReader, " ");
break;
case "JOURNAL":
reference.Journal = ParseMultiLineData(mbfReader, " ");
break;
case "REMARK":
reference.Remarks = ParseMultiLineData(mbfReader, " ");
break;
case "MEDLINE":
reference.Medline = ParseMultiLineData(mbfReader, " ");
break;
case "PUBMED":
reference.PubMed = ParseMultiLineData(mbfReader, " ");
break;
default:
string message = String.Format(
CultureInfo.CurrentCulture,
Properties.Resource.ParserInvalidReferenceField,
mbfReader.LineHeader);
Trace.Report(message);
throw new InvalidDataException(message);
}
}
else
{
// add last reference
if (reference != null)
{
referenceList.Add(reference);
}
// don't go to next line; current line still needs to be processed
break;
}
}
}
// parses everything before the features section
private void ParseHeaders(MBFTextReader mbfReader, ref Sequence sequence)
{
GenBankMetadata metadata = (GenBankMetadata)sequence.Metadata[Helper.GenBankMetadataKey];
string data = string.Empty;
string[] tokens = null;
// set data indent for headers
mbfReader.DataIndent = _dataIndent;
// only allow one locus line
bool haveParsedLocus = false;
// parse until we hit the features or sequence section
bool haveFinishedHeaders = false;
while (mbfReader.HasLines && !haveFinishedHeaders)
{
switch (mbfReader.LineHeader)
{
case "LOCUS":
if (haveParsedLocus)
{
string message = String.Format(
CultureInfo.CurrentCulture,
Properties.Resource.ParserSecondLocus,
mbfReader.LocationString);
Trace.Report(message);
throw new InvalidDataException(message);
}
ParseLocusByTokens(mbfReader, ref sequence);
metadata = (GenBankMetadata)sequence.Metadata[Helper.GenBankMetadataKey];
haveParsedLocus = true;
// don't go to next line; current line still needs to be processed
break;
case "VERSION":
tokens = mbfReader.LineData.Split(new char[] { ' ' },
StringSplitOptions.RemoveEmptyEntries);
// first token contains accession and version
Match m = Regex.Match(tokens[0], @"^(?<accession>\w+)\.(?<version>\d+)$");
metadata.Version = new GenBankVersion();
if (m.Success)
{
metadata.Version.Version = m.Groups["version"].Value;
// The first token in the data from the accession line is referred to as
// the primary accession number, and should be the one used here in the
// version line.
string versionLineAccession = m.Groups["accession"].Value;
if (metadata.Accession == null)
{
ApplicationLog.WriteLine("WARN: VERSION processed before ACCESSION");
}
else
{
if (!versionLineAccession.Equals(metadata.Accession.Primary))
{
ApplicationLog.WriteLine("WARN: VERSION tag doesn't match ACCESSION");
}
else
{
metadata.Version.Accession = metadata.Accession.Primary;
}
}
}
// second token contains primary ID
m = Regex.Match(tokens[1], @"^GI:(?<primaryID>.*)");
if (m.Success)
{
metadata.Version.GINumber = m.Groups["primaryID"].Value;
}
mbfReader.GoToNextLine();
break;
case "PROJECT":
tokens = mbfReader.LineData.Split(':');
if (tokens.Length == 2)
{
metadata.Project = new ProjectIdentifier();
metadata.Project.Name = tokens[0];
tokens = tokens[1].Split(',');
for (int i = 0; i < tokens.Length; i++)
{
metadata.Project.Numbers.Add(tokens[i]);
}
}
else
{
ApplicationLog.WriteLine("WARN: unexpected PROJECT header: " + mbfReader.Line);
}
mbfReader.GoToNextLine();
break;
case "SOURCE":
ParseSource(mbfReader, ref sequence);
metadata = (GenBankMetadata)sequence.Metadata[Helper.GenBankMetadataKey];
// don't go to next line; current line still needs to be processed
break;
case "REFERENCE":
ParseReferences(mbfReader, ref sequence); // can encounter more than one
metadata = (GenBankMetadata)sequence.Metadata[Helper.GenBankMetadataKey];
// don't go to next line; current line still needs to be processed
break;
case "COMMENT":
ParseComments(mbfReader, ref sequence); // can encounter more than one
metadata = (GenBankMetadata)sequence.Metadata[Helper.GenBankMetadataKey];
// don't go to next line; current line still needs to be processed
break;
case "PRIMARY":
// This header is followed by sequence info in a table format that could be
// stored in a custom object. The first line contains column headers.
// For now, just validate the presence of the headers, and save the data
// as a string.
tokens = mbfReader.LineData.Split("\t ".ToCharArray(), StringSplitOptions.RemoveEmptyEntries);
// Validating for minimum two headers.
if (tokens.Length != 4)
{
string message = String.Format(
CultureInfo.CurrentCulture,
Properties.Resource.ParserPrimaryLineError,
mbfReader.Line);
Trace.Report(message);
throw new InvalidDataException(message);
}
string primaryData = ParseMultiLineData(mbfReader, Environment.NewLine);
metadata.Primary = primaryData;
// don't go to next line; current line still needs to be processed
break;
// all the following are extracted the same way - possibly multiline
case "DEFINITION":
metadata.Definition = ParseMultiLineData(mbfReader, " ");
break;
case "ACCESSION":
data = ParseMultiLineData(mbfReader, " ");
metadata.Accession = new GenBankAccession();
string[] accessions = data.Split(' ');
metadata.Accession.Primary = accessions[0];
for (int i = 1; i < accessions.Length; i++)
{
metadata.Accession.Secondary.Add(accessions[i]);
}
break;
case "DBLINK":
tokens = mbfReader.LineData.Split(':');
if (tokens.Length == 2)
{
metadata.DBLink = new CrossReferenceLink();
if (string.Compare(tokens[0],
CrossReferenceType.Project.ToString(),
StringComparison.OrdinalIgnoreCase) == 0)
{
metadata.DBLink.Type = CrossReferenceType.Project;
}
else
{
metadata.DBLink.Type = CrossReferenceType.TraceAssemblyArchive;
}
tokens = tokens[1].Split(',');
for (int i = 0; i < tokens.Length; i++)
{
metadata.DBLink.Numbers.Add(tokens[i]);
}
}
else
{
ApplicationLog.WriteLine("WARN: unexpected DBLINK header: " + mbfReader.Line);
}
mbfReader.GoToNextLine();
break;
case "DBSOURCE":
metadata.DBSource = ParseMultiLineData(mbfReader, " ");
break;
case "KEYWORDS":
metadata.Keywords = ParseMultiLineData(mbfReader, " ");
break;
case "SEGMENT":
data = ParseMultiLineData(mbfReader, " ");
string delimeter = "of";
tokens = data.Split(delimeter.ToCharArray(), StringSplitOptions.RemoveEmptyEntries);
int outvalue;
if (tokens.Length == 2)
{
metadata.Segment = new SequenceSegment();
if (int.TryParse(tokens[0].Trim(), out outvalue))
{
metadata.Segment.Current = outvalue;
}
else
{
ApplicationLog.WriteLine("WARN: unexpected SEGMENT header: " + mbfReader.Line);
}
if (int.TryParse(tokens[1].Trim(), out outvalue))
{
metadata.Segment.Count = outvalue;
}
else
{
ApplicationLog.WriteLine("WARN: unexpected SEGMENT header: " + mbfReader.Line);
}
}
else
{
ApplicationLog.WriteLine("WARN: unexpected SEGMENT header: " + mbfReader.Line);
}
break;
// all the following indicate sections beyond the headers parsed by this method
case "FEATURES":
case "BASE COUNT":
case "ORIGIN":
case "CONTIG":
haveFinishedHeaders = true;
break;
default:
ApplicationLog.WriteLine(ToString() + "WARN: unknown {0} -> {1}", mbfReader.LineHeader, mbfReader.LineData);
string errMessage = String.Format(
CultureInfo.CurrentCulture,
Properties.Resource.ParseHeaderError,
mbfReader.LineHeader);
Trace.Report(errMessage);
throw new InvalidDataException(errMessage);
}
}
// check for required features
if (!haveParsedLocus)
{
string message = string.Format(CultureInfo.CurrentCulture, Resource.INVALID_INPUT_FILE, this.Name);
Trace.Report(message);
throw new InvalidDataException(message);
}
}
private void WriteLocus(ISequence sequence, TextWriter writer)
{
// determine molecule and seqiemce type
GenBankMetadata metadata = (GenBankMetadata)sequence.Metadata[Helper.GenBankMetadataKey];
GenBankLocusInfo locusInfo = null;
if (metadata != null)
{
locusInfo = metadata.Locus;
}
string molType = sequence.MoleculeType.ToString();
string seqType;
if (sequence.MoleculeType != MoleculeType.Invalid)
{
if (molType == MoleculeType.Protein.ToString())
{
seqType = "aa";
molType = string.Empty; // protein files don't use molecule type
}
else
{
seqType = "bp";
}
}
else
{
if (sequence.Alphabet == Alphabets.Protein)
{
seqType = "aa";
molType = string.Empty; // protein files don't use molecule type
}
else
{
seqType = "bp";
if (sequence.Alphabet == Alphabets.DNA)
{
molType = MoleculeType.DNA.ToString();
}
else
{
molType = MoleculeType.RNA.ToString();
}
}
}
// retrieve metadata fields
string strandType = string.Empty;
string strandTopology = string.Empty;
string division = string.Empty;
DateTime date = DateTime.Now;
if (locusInfo != null)
{
strandType = Helper.GetStrandType(locusInfo.Strand);
strandTopology = Helper.GetStrandTopology(locusInfo.StrandTopology);
if (locusInfo.DivisionCode != SequenceDivisionCode.None)
{
division = locusInfo.DivisionCode.ToString();
}
date = locusInfo.Date;
}
writer.WriteLine("{0,-12}{1,-16} {2,11} {3} {4,3}{5,-6} {6,-8} {7,3} {8}",
"LOCUS",
sequence.ID,
sequence.Count,
seqType,
strandType,
molType,
strandTopology,
division,
date.ToString("dd-MMM-yyyy").ToUpper());
}