private void FI_reduseC(CellToolDK.TifFileInfo fi)
{
List <Color> l = new List <Color>();
for (int i = 0; i < fi.sizeC; i++)
{
if (i < fi.LutList.Count)
{
l.Add(fi.LutList[i]);
}
else
{
l.Add(System.Drawing.Color.White);
}
}
fi.LutList = l;
fi.cValue = 0;
#region Segmentation variables
fi.histogramArray = null;
fi.adjustedLUT = null;
fi.MaxBrightness = null;
fi.MinBrightness = null;
fi.SegmentationCBoxIndex = new int[fi.sizeC];
fi.SegmentationProtocol = new int[fi.sizeC];
fi.thresholdsCBoxIndex = new int[fi.sizeC];
fi.sumHistogramChecked = new bool[fi.sizeC];
fi.thresholdValues = new int[fi.sizeC][];
fi.thresholdColors = new Color[fi.sizeC][];
fi.RefThresholdColors = new Color[fi.sizeC][];
fi.thresholds = new int[fi.sizeC];
fi.SpotColor = new Color[fi.sizeC];
fi.RefSpotColor = new Color[fi.sizeC];
fi.SelectedSpotThresh = new int[fi.sizeC];
fi.SpotThresh = new int[fi.sizeC];
fi.typeSpotThresh = new int[fi.sizeC];
fi.SpotTailType = new string[fi.sizeC];
fi.spotSensitivity = new int[fi.sizeC];
fi.roiList = new List <CellToolDK.ROI> [fi.sizeC];
fi.tracking_MaxSize = new int[fi.sizeC];
fi.tracking_MinSize = new int[fi.sizeC];
fi.tracking_Speed = new int[fi.sizeC];
for (int i = 0; i < fi.sizeC; i++)
{
fi.sumHistogramChecked[i] = false;
fi.thresholdValues[i] = new int[5];
fi.thresholdColors[i] = new Color[]
{ Color.Transparent, Color.Transparent, Color.Transparent, Color.Transparent, Color.Transparent };
fi.RefThresholdColors[i] = new Color[]
{ Color.Black, Color.Orange, Color.Green, Color.Blue, Color.Magenta };
fi.SpotColor[i] = Color.Red;
fi.RefSpotColor[i] = Color.Red;
fi.SpotTailType[i] = "<";
fi.spotSensitivity[i] = 100;
fi.tracking_MaxSize[i] = 10000;
fi.tracking_MinSize[i] = 5;
fi.tracking_Speed[i] = 5;
}
#endregion Segmentation variables
}
private void CellToolDKtoFI(CellToolDK.TifFileInfo fi, TifFileInfo oldFI)
{
if (fi == null)
{
oldFI = null;
return;
}
TifFileInfo newFI = oldFI;
newFI.zoom = fi.zoom;
newFI.Xposition = fi.Xposition;
newFI.Yposition = fi.Yposition;
//Chart
newFI.xAxisTB = fi.xAxisTB;
newFI.yAxisTB = fi.yAxisTB;
//Tracking
newFI.tracking_MaxSize = fi.tracking_MaxSize;
newFI.tracking_MinSize = fi.tracking_MinSize;
newFI.tracking_Speed = fi.tracking_Speed;
/// <summary>
/// Segmentation
/// </summary>
//filter history
newFI.FilterHistory = fi.FilterHistory;
//controls
newFI.DataSourceInd = fi.DataSourceInd;
newFI.SegmentationProtocol = fi.SegmentationProtocol;
newFI.SegmentationCBoxIndex = fi.SegmentationCBoxIndex;
newFI.thresholdsCBoxIndex = fi.thresholdsCBoxIndex;
newFI.sumHistogramChecked = fi.sumHistogramChecked;
//spotdetector controls
newFI.SpotThresh = fi.SpotThresh;
newFI.SpotColor = fi.SpotColor;
newFI.RefSpotColor = fi.RefSpotColor;
newFI.SelectedSpotThresh = fi.SelectedSpotThresh;
newFI.typeSpotThresh = fi.typeSpotThresh;
newFI.SpotTailType = fi.SpotTailType;
newFI.spotSensitivity = fi.spotSensitivity;
//specific values
newFI.thresholds = fi.thresholds;
newFI.thresholdValues = fi.thresholdValues;
newFI.thresholdColors = fi.thresholdColors;
newFI.RefThresholdColors = fi.RefThresholdColors;
//BandC
newFI.autoDetectBandC = fi.autoDetectBandC;
newFI.applyToAllBandC = fi.applyToAllBandC;
//
newFI.selectedPictureBoxColumn = fi.selectedPictureBoxColumn;
//History
newFI.History = fi.History;
newFI.HistoryPlace = fi.HistoryPlace;
newFI.undo = fi.undo;
newFI.redo = fi.redo;
newFI.delHist = fi.delHist;
//info var
newFI.frame = fi.frame;
newFI.zValue = fi.zValue;
newFI.cValue = fi.cValue;
newFI.original = fi.original;
//Not available
newFI.loaded = fi.loaded;
newFI.available = fi.available;
newFI.selected = fi.selected;
newFI.openedImages = fi.openedImages;
//tif image
newFI.image8bit = fi.image8bit;
newFI.image16bit = fi.image16bit;
newFI.image8bitFilter = fi.image8bitFilter;
newFI.image16bitFilter = fi.image16bitFilter;
//tif tags
newFI.Dir = fi.Dir;
newFI.seriesCount = fi.seriesCount;
newFI.imageCount = fi.imageCount;
newFI.sizeX = fi.sizeX;
newFI.sizeY = fi.sizeY;
newFI.sizeZ = fi.sizeZ;
newFI.umZ = fi.umZ;
newFI.umXY = fi.umXY;
newFI.sizeC = fi.sizeC;
newFI.sizeT = fi.sizeT;
newFI.bitsPerPixel = fi.bitsPerPixel;
newFI.dimensionOrder = fi.dimensionOrder;
newFI.pixelType = fi.pixelType;
newFI.FalseColored = fi.FalseColored;
newFI.isIndexed = fi.isIndexed;
newFI.MetadataComplete = fi.MetadataComplete;
newFI.DatasetStructureDescription = fi.DatasetStructureDescription;
newFI.LutList = fi.LutList;
newFI.TimeSteps = fi.TimeSteps;
newFI.Micropoint = fi.Micropoint;
//Metadata protocol info
newFI.FileDescription = fi.FileDescription;
newFI.xCompensation = fi.xCompensation;
newFI.yCompensation = fi.yCompensation;
//properties
newFI.histogramArray = fi.histogramArray;
newFI.MinBrightness = fi.MinBrightness;
newFI.MaxBrightness = fi.MaxBrightness;
newFI.adjustedLUT = fi.adjustedLUT;
//RoiManager
newFI.roiList = new List <ROI> [fi.roiList.Length];
for (int c = 0; c < fi.roiList.Length; c++)
{
if (fi.roiList[c] != null)
{
newFI.roiList[c] = new List <ROI>();
foreach (CellToolDK.ROI roi in fi.roiList[c])
{
ROI newROI = CellToolDKtoROI(roi);
newFI.roiList[c].Add(newROI);
RoiMeasure.Measure(newROI, newFI, c, IA);
}
}
}
newFI.ROICounter = fi.ROICounter;
}
private void PlugIn_Load(string path)
{
TifFileInfo oldFI = null;
try
{
oldFI = IA.TabPages.TabCollections[IA.TabPages.SelectedIndex].tifFI;
}
catch { }
CellToolDK.TifFileInfo fi = FItoCellToolDK(oldFI);
var DLL = Assembly.LoadFile(path);
foreach (Type type in DLL.GetExportedTypes())
{
try
{
Transmiter e = new Transmiter();
e.Changed += new TransmiterEventHandler(delegate(object o, TransmiterEventArgs a)
{
try
{
if (fi != null && oldFI != null)
{
if (fi.sizeC != oldFI.sizeC)
{
oldFI.sizeC = fi.sizeC;
FI_reduseC(fi);
FI_reduseC(oldFI);
}
CellToolDKtoFI(fi, oldFI);
if (oldFI.sizeZ > 1)
{
IA.TabPages.zTrackBar.Refresh(oldFI.zValue + 1, 1, oldFI.sizeZ);
IA.TabPages.zTrackBar.Panel.Visible = true;
}
else
{
IA.TabPages.zTrackBar.Panel.Visible = false;
}
if (oldFI.sizeT > 1)
{
IA.TabPages.tTrackBar.Refresh(oldFI.frame + 1, 1, oldFI.sizeT);
IA.TabPages.tTrackBar.Panel.Visible = true;
}
else
{
IA.TabPages.tTrackBar.Panel.Visible = false;
}
}
}
catch { MessageBox.Show("Error with reporting back!"); }
IA.ReloadImages();
});
var c = Activator.CreateInstance(type);
try
{
type.InvokeMember("Input", BindingFlags.InvokeMethod, null, c, new object[] { fi, e });
}
catch { MessageBox.Show("Input void is not avaliable!"); }
break;
}
catch { }
}
}