private void bgWorkerGenerateImages_DoWork(object sender, DoWorkEventArgs e)
{
foreach (string resultFile in ResultFiles)
{
currentFile = resultFile;
if (resultFile.Contains("_FullList.csv"))
{
currentFile = resultFile.Remove(resultFile.IndexOf("_FullList.csv")) + ".csv";
}
bgWorkerGenerateImages.ReportProgress(0);
string FullListFile = currentFile.Replace(".csv", "_FullList.csv");
string QuantFile = currentFile.Replace(".csv", "_Quant.csv");
//Get individual image
if (isIndividualImg && File.Exists(FullListFile))
{
GenerateImages.GenGlycanLcImg(
FullListFile,
ExportFilePath + "\\" + Path.GetFileNameWithoutExtension(currentFile),
out lstImgErrors);
}
//Get Quant Image
if (isQuantImg && File.Exists(QuantFile))
{
GenerateImages.GenQuantImg(
QuantFile,
LabelingMethod,
ExportFilePath + "\\" + Path.GetFileNameWithoutExtension(currentFile));
}
CompletedCount = CompletedCount + 1;
bgWorkerGenerateImages.ReportProgress(0);
}
}
private void btnMergeTest_Click(object sender, EventArgs e)
{
GenerateImages.GenQuantImg(@"E:\!MultiGlycanbatch\1\1_Quant.csv", enumGlycanLabelingMethod.MultiplexPermethylated, @"E:\!MultiGlycanbatch\1");
//StreamReader sr = new StreamReader(@"D:\Dropbox\for_Yunli_Hu\b1_19_1_07142012-121002 1349_FullList.csv");
//string tmp = sr.ReadLine();
//List<ClusteredPeak> clu = new List<ClusteredPeak>();
//do
//{
// tmp = sr.ReadLine();
// string[] tmpArray = tmp.Split(',');
// ClusteredPeak tnpCluPeak = new ClusteredPeak(Convert.ToInt32(tmpArray[1]));
// tnpCluPeak.StartTime = Convert.ToDouble(tmpArray[0]);
// tnpCluPeak.EndTime = Convert.ToDouble(tmpArray[0]);
// tnpCluPeak.EndScan = Convert.ToInt32(tmpArray[1]);
// tnpCluPeak.Intensity = Convert.ToSingle(tmpArray[2]);
// tnpCluPeak.GlycanComposition = new COL.GlycoLib.GlycanCompound(
// Convert.ToInt32(tmpArray[8]),
// Convert.ToInt32(tmpArray[9]),
// Convert.ToInt32(tmpArray[10]),
// Convert.ToInt32(tmpArray[11]));
// tnpCluPeak.Charge = Convert.ToInt32(Math.Ceiling( Convert.ToSingle(tmpArray[12]) / Convert.ToSingle(tmpArray[3])));
// clu.Add(tnpCluPeak);
//} while (!sr.EndOfStream);
//sr.Close();
////MultiNGlycanESI.MergeCluster(clu, 8.0);
}
private void bgWorker_Process_RunWorkerCompleted(object sender, RunWorkerCompletedEventArgs e)
{
try
{
// _MultiNGlycan.ExportParametersToExcel();
if (DoLog)
{
Logger.WriteLog("Start merge peaks");
}
lblStatus.Text = "Mergeing Peaks";
if (_MultiNGlycan.LabelingMethod == enumGlycanLabelingMethod.MultiplexPermethylated)
{
SecondPassedForMultiplexPermethylated.Processing(ref _MultiNGlycan);
}
//if (_MultiNGlycan.TwoPassedID)
//{
// // _MultiNGlycan.Merge2PassedCluster();
// _MultiNGlycan.MergeSingleScanResultToPeak();
//}
//else
//{
// _MultiNGlycan.MergeCluster();
_MultiNGlycan.MergeSingleScanResultToPeak();
_MultiNGlycan.SolveDuplicateAssignment();
_MultiNGlycan.MergeSingleScanResultToPeak();
//}
if (DoLog)
{
Logger.WriteLog("End merge peaks");
}
if (_MultiNGlycan.GlycanLCorderExist)
{
_MultiNGlycan.ApplyLCordrer();
}
if (!Directory.Exists(_MultiNGlycan.ExportFilePath + "\\Pic") && (_MultiNGlycan.IndividualImgs || _MultiNGlycan.QuantificationImgs))
{
Directory.CreateDirectory(_MultiNGlycan.ExportFilePath + "\\Pic");
}
if (_MultiNGlycan.LabelingMethod == GlycoLib.enumGlycanLabelingMethod.MultiplexPermethylated)
{
_MultiNGlycan.EstimatePurity();
foreach (GlycoLib.enumLabelingTag tag in _MultiNGlycan.LabelingRatio.Keys)
{
if (tag == enumLabelingTag.MP_CH3 || !_MultiNGlycan.HasEstimatePurity((tag)))
{
continue;
}
_MultiNGlycan.GetPurityEstimateImage(tag, _MultiNGlycan.ExportFilePath + "\\Pic\\EstimatePurity_" + tag.ToString() + ".png");
}
//Correct Intensity;
//_MultiNGlycan.CorrectIntensityByIsotope();
}
if (DoLog)
{
;
Logger.WriteLog("Start export");
}
lblStatus.Text = "Exporting";
_MultiNGlycan.ExportToCSV();
//Get individual image
if (_MultiNGlycan.IndividualImgs &&
File.Exists(_MultiNGlycan.ExportFilePath + "\\" + Path.GetFileName(_MultiNGlycan.ExportFilePath) +
"_FullList.csv"))
{
//GenerateImages.GenGlycanLcImg(
// _MultiNGlycan.ExportFilePath + "\\" + Path.GetFileName(_MultiNGlycan.ExportFilePath) +"_FullList.csv",
// _MultiNGlycan.ExportFilePath);
GenerateImages.GenGlycanLcImg(_MultiNGlycan);
}
//Get Quant Image
if (_MultiNGlycan.LabelingMethod != enumGlycanLabelingMethod.None && _MultiNGlycan.QuantificationImgs &&
File.Exists(_MultiNGlycan.ExportFilePath + "\\" + Path.GetFileName(_MultiNGlycan.ExportFilePath) +
"_Quant.csv"))
{
GenerateImages.GenQuantImg(
_MultiNGlycan.ExportFilePath + "\\" + Path.GetFileName(_MultiNGlycan.ExportFilePath) + "_Quant.csv",
_MultiNGlycan.LabelingMethod,
_MultiNGlycan.ExportFilePath);
}
//_MultiNGlycan.ExportToExcel();
if (DoLog)
{
Logger.WriteLog("End export");
}
TimeSpan TDiff = DateTime.Now.Subtract(Start);
lblStatus.Text = "Finish in " + TDiff.TotalMinutes.ToString("0.00") + " mins";
lblNumberOfMerge.Text = _MultiNGlycan.MergedResultList.Count.ToString();
progressBar1.Value = 100;
lblPercentage.Text = "100%";
FlashWindow.Flash(this);
this.Text = "Done";
if (DoLog)
{
Logger.WriteLog("End process each scan");
}
}
catch (Exception ex)
{
throw ex;
}
}
