By default, the load balancer routes requests to registered targets using the protocol and port number for the target group. Alternatively, you can override the port for a target when you register it.
The target must be in the virtual private cloud (VPC) that you specified for the target group. If the target is an EC2 instance, it can't be in the stopped
or running
state when you register it.
To remove a target from a target group, use DeregisterTargets.