public Mapper(SequenceAlignment alignment, string connectionString, string databaseName)
{
DatabaseName = databaseName;
ConnectionString = connectionString;
MappedAlignment = alignment;
MappedSuccessfully = false;
}
static void Main(string[] args)
{
List <string> alphabet = new List <string>(new[] { "H", "A", "P", "L", "E", "-" });
string[] X = { "", "A", "P", "P", "L", "E" };
string[] Y = { "", "H", "A", "P", "E" };
SequenceAlignment seqAlign = new SequenceAlignment(X, Y);
seqAlign.ScoreMatrix(alphabet, 1, -1, -1);
Dictionary <string, Dictionary <string, int> > scores = seqAlign.scores;
foreach (string k in alphabet)
{
Console.Write(k + " ");
foreach (KeyValuePair <string, int> p in scores[k])
{
Console.Write(p.Key + " " + p.Value + " ");
}
Console.WriteLine();
}
Console.WriteLine();
seqAlign.AlignScore();
Dictionary <string, string> ans = seqAlign.AlignSequence();
Console.WriteLine(ans["X"]);
Console.WriteLine(ans["Y"]);
Console.ReadLine();
}
public void ValidateSequenceAlignmentToString()
{
ISequenceAligner aligner = SequenceAligners.NeedlemanWunsch;
IAlphabet alphabet = Alphabets.Protein;
string origSequence1 = "KRIPKSQNLRSIHSIFPFLEDKLSHLN";
string origSequence2 = "LNIPSLITLNKSIYVFSKRKKRLSGFLHN";
// Create input sequences
var inputSequences = new List <ISequence>();
inputSequences.Add(new Sequence(alphabet, origSequence1));
inputSequences.Add(new Sequence(alphabet, origSequence2));
// Get aligned sequences
IList <ISequenceAlignment> alignments = aligner.Align(inputSequences);
ISequenceAlignment alignment = new SequenceAlignment();
for (int ialigned = 0; ialigned < alignments[0].AlignedSequences.Count; ialigned++)
{
alignment.AlignedSequences.Add(alignments[0].AlignedSequences[ialigned]);
}
foreach (string key in alignments[0].Metadata.Keys)
{
alignment.Metadata.Add(key, alignments[0].Metadata[key]);
}
string actualSequenceAlignmentString = alignment.ToString();
string ExpectedSequenceAlignmentString = this.utilityObj.xmlUtil.GetTextValue(Constants.ToStringNodeName,
Constants
.SequenceAlignmentExpectedNode);
Assert.AreEqual(ExpectedSequenceAlignmentString.Replace("\\r\\n", ""),
actualSequenceAlignmentString.Replace(System.Environment.NewLine, ""));
}
public void TestSequenceAlignmentToString()
{
ISequenceAligner aligner = SequenceAligners.NeedlemanWunsch;
IAlphabet alphabet = Alphabets.Protein;
const string origSequence1 = "KRIPKSQNLRSIHSIFPFLEDKLSHLN";
const string origSequence2 = "LNIPSLITLNKSIYVFSKRKKRLSGFLHN";
// Create input sequences
var inputSequences = new List <ISequence>
{
new Sequence(alphabet, origSequence1),
new Sequence(alphabet, origSequence2)
};
// Get aligned sequences
IList <ISequenceAlignment> alignments = aligner.Align(inputSequences);
ISequenceAlignment alignment = new SequenceAlignment();
foreach (var alignedSequence in alignments[0].AlignedSequences)
{
alignment.AlignedSequences.Add(alignedSequence);
}
const string expected = "XXIPXXXXLXXXXXXFXXXXXXLSXXLHN\r\n" +
"KRIPKSQNLRSIHSIFPFLEDKLSHL--N\r\n" +
"LNIPSLITLNKSIYVFSKRKKRLSGFLHN\r\n\r\n";
Assert.AreEqual(expected.Replace("\r\n", Environment.NewLine), alignment.ToString());
}
public void TestSequenceAlignmentToString()
{
ISequenceAligner aligner = SequenceAligners.NeedlemanWunsch;
IAlphabet alphabet = Alphabets.Protein;
string origSequence1 = "KRIPKSQNLRSIHSIFPFLEDKLSHLN";
string origSequence2 = "LNIPSLITLNKSIYVFSKRKKRLSGFLHN";
// Create input sequences
List <ISequence> inputSequences = new List <ISequence>();
inputSequences.Add(new Sequence(alphabet, origSequence1));
inputSequences.Add(new Sequence(alphabet, origSequence2));
// Get aligned sequences
IList <ISequenceAlignment> alignments = aligner.Align(inputSequences);
ISequenceAlignment alignment = new SequenceAlignment();
for (int ialigned = 0; ialigned < alignments[0].AlignedSequences.Count; ialigned++)
{
alignment.AlignedSequences.Add(alignments[0].AlignedSequences[ialigned]);
}
foreach (string key in alignments[0].Metadata.Keys)
{
alignment.Metadata.Add(key, alignments[0].Metadata[key]);
}
string actualSequenceAlignmentString = alignment.ToString();
string ExpectedSequenceAlignmentString = "XXIPXXXXLXXXXXXFXXXXXXLSGFXXN\r\nKRIPKSQNLRSIHSIFPFLEDKLS--HLN\r\nLNIPSLITLNKSIYVFSKRKKRLSGFLHN\r\n\r\n";
Assert.AreEqual(ExpectedSequenceAlignmentString, actualSequenceAlignmentString);
}
public void Write(IEnumerable <Read> reads, Stream targetStream)
{
if (reads == null)
{
reads = new Read[] {}
}
;
var _samFormatter = samOutput ? (IFormatter <ISequenceAlignment>) new SAMFormatter() : new BAMFormatter();
var _samAlignedSequences = reads.Select(SamReadsConverter.Convert).ToList();
var _sa = new SequenceAlignment();
foreach (var _samAlignedSequence in _samAlignedSequences)
{
_sa.AlignedSequences.Add(_samAlignedSequence);
}
var _refSequenceName = "1";
if (_samAlignedSequences.Count != 0)
{
_refSequenceName = _samAlignedSequences[0].RName;
}
_sa.Metadata.Add("SAMAlignmentHeader",
new SAMAlignmentHeader
{
ReferenceSequences =
{
new ReferenceSequenceInfo
{
Name = _refSequenceName
}
}
});
_samFormatter.Format(targetStream, _sa);
}
}
public AlignmentViewModel(SequenceAlignment alignment)
{
_alignment = alignment;
_sequences = new MTObservableCollection <SequenceViewModel>();
MapToRCADCommand = new DelegatingCommand <rCADConnection>(MapAlignmentToRCAD, (a) => (!MappingToRCAD && !IsMappedToRCAD && a != null));
//MapToRCADCommand = new DelegatingCommand<string>(MapAlignmentToRCAD, (a) => (!MappingToRCAD && !IsMappedToRCAD && a != null));
LoadToRCADCommand = new DelegatingCommand <string>(LoadAlignmentToRCAD, (a) => (!MappingToRCAD && IsMappedToRCAD && _alignmentToRCADMapping != null && !LoadingToRCADFailed && !LoadingToRCAD && !IsLoadedToRCAD));
//LoadToRCADCommand = new DelegatingCommand(LoadAlignmentToRCAD, () => (!MappingToRCAD && IsMappedToRCAD && _alignmentToRCADMapping != null && !LoadingToRCADFailed && !LoadingToRCAD && !IsLoadedToRCAD));
Initialize();
}
public void ValidateSequenceAlignmentGetObjectData()
{
SerializationInfo info =
new SerializationInfo(typeof(Sequence),
new FormatterConverter());
StreamingContext context =
new StreamingContext(StreamingContextStates.All);
SequenceAlignment seqAlignmentObj = new SequenceAlignment();
seqAlignmentObj.GetObjectData(info, context);
Assert.AreEqual(4, info.MemberCount);
}
private void LoadAlignmentWorkerCompleted(object sender, RunWorkerCompletedEventArgs e)
{
if (e.Result == null)
{
LoadingAlignmentStatusMessage = LOADING_ALIGNMENT_FAILED_MESSAGE;
_loadingAlignment = false;
CommandManager.InvalidateRequerySuggested();
return;
}
SequenceAlignment aln = e.Result as SequenceAlignment;
_loadingAlignment = false;
CommandManager.InvalidateRequerySuggested();
LoadingAlignmentStatusMessage = FINISHED_LOADING_ALIGNMENT_MESSAGE;
Alignment = new AlignmentViewModel(aln);
}
/// <summary>
/// Validate sequence alignment instance using different aligners
/// </summary>
/// <param name="nodeName">xml node name</param>
/// <param name="aligner">sw/nw/pw aligners</param>
private void ValidateSequenceAlignmentCtor(string nodeName, ISequenceAligner aligner)
{
IAlphabet alphabet = Utility.GetAlphabet(this.utilityObj.xmlUtil.GetTextValue(nodeName,
Constants.AlphabetNameNode));
string origSequence1 = this.utilityObj.xmlUtil.GetTextValue(nodeName, Constants.SequenceNode1);
string origSequence2 = this.utilityObj.xmlUtil.GetTextValue(nodeName, Constants.SequenceNode2);
// Create input sequences
var inputSequences = new List <ISequence>();
inputSequences.Add(new Sequence(alphabet, origSequence1));
inputSequences.Add(new Sequence(alphabet, origSequence2));
// Get aligned sequences
IList <ISequenceAlignment> alignments = aligner.Align(inputSequences);
ISequenceAlignment alignment = new SequenceAlignment();
for (int ialigned = 0; ialigned < alignments[0].AlignedSequences.Count; ialigned++)
{
alignment.AlignedSequences.Add(alignments[0].AlignedSequences[ialigned]);
}
foreach (string key in alignments[0].Metadata.Keys)
{
alignment.Metadata.Add(key, alignments[0].Metadata[key]);
}
// Validate the properties
for (int ialigned = 0; ialigned < alignments[0].AlignedSequences.Count; ialigned++)
{
Assert.AreEqual(alignments[0].AlignedSequences[ialigned].Sequences[0].ToString(),
alignment.AlignedSequences[ialigned].Sequences[0].ToString());
}
foreach (string key in alignments[0].Metadata.Keys)
{
Assert.AreEqual(alignments[0].Metadata[key], alignment.Metadata[key]);
}
ApplicationLog.WriteLine(@"Alignment BVT : Validation of sequence alignment ctor completed successfully");
}
private void LoadAlignmentWorker(object sender, DoWorkEventArgs e)
{
if (e.Argument == null)
{
return;
}
SequenceAlignmentLoaderArgs args = e.Argument as SequenceAlignmentLoaderArgs;
if (args != null)
{
if (args.AlignmentFile == null)
{
return;
}
if (args.AlignmentType == AlignmentType.CRW)
{
ISequenceAlignmentLoader loader = new CRWSequenceAlignmentLoader();
SequenceAlignment aln = loader.Load(args.AlignmentFile);
e.Result = aln;
}
}
}
/// <summary>
/// Parses a single Nexus text from a reader into a sequence.
/// </summary>
/// <param name="bioReader">A reader for a biological sequence text.</param>
/// <param name="isReadOnly">
/// Flag to indicate whether the resulting sequence alignment should be in readonly mode or not.
/// If this flag is set to true then the resulting sequence's isReadOnly property
/// will be set to true, otherwise it will be set to false.</param>
/// <returns>A new Sequence instance containing parsed data.</returns>
protected ISequenceAlignment ParseOneWithSpecificFormat(BioTextReader bioReader, bool isReadOnly)
{
if (bioReader == null)
{
throw new ArgumentNullException("bioReader");
}
ParseHeader(bioReader);
string message = string.Empty;
ISequenceAlignment sequenceAlignment = new SequenceAlignment();
sequenceAlignment.AlignedSequences.Add(new AlignedSequence());
IList <string> ids = null;
bool isInBlock = true;
if (bioReader.Line.StartsWith("begin", StringComparison.OrdinalIgnoreCase))
{
while (bioReader.HasLines && isInBlock)
{
if (string.IsNullOrEmpty(bioReader.Line.Trim()))
{
bioReader.GoToNextLine();
continue;
}
string blockName = GetTokens(bioReader.Line)[1];
switch (blockName.ToUpper(CultureInfo.InvariantCulture))
{
case "TAXA":
case "TAXA;":
// This block contains the count of sequence & title of each sequence
ids = (IList <string>)ParseTaxaBlock(bioReader);
break;
case "CHARACTERS":
case "CHARACTERS;":
// Block contains sequences
Dictionary <string, string> dataSet = ParseCharacterBlock(bioReader, ids);
IAlphabet alignmentAlphabet = null;
string data = string.Empty;
foreach (string ID in ids)
{
IAlphabet alphabet = Alphabet;
Sequence sequence = null;
data = dataSet[ID];
if (null == alphabet)
{
alphabet = _basicParser.IdentifyAlphabet(alphabet, data);
if (null == alphabet)
{
message = string.Format(
CultureInfo.InvariantCulture,
Resource.InvalidSymbolInString,
data);
throw new InvalidDataException(message);
}
else
{
if (null == alignmentAlphabet)
{
alignmentAlphabet = alphabet;
}
else
{
if (alignmentAlphabet != alphabet)
{
message = string.Format(
CultureInfo.InvariantCulture,
Properties.Resource.SequenceAlphabetMismatch);
throw new InvalidDataException(message);
}
}
}
}
if (Encoding == null)
{
sequence = new Sequence(alphabet, data);
}
else
{
sequence = new Sequence(alphabet, Encoding, data);
}
sequence.IsReadOnly = isReadOnly;
sequence.ID = ID;
sequenceAlignment.AlignedSequences[0].Sequences.Add(sequence);
}
break;
case "END":
case "END;":
// Have reached the end of block
isInBlock = false;
break;
default:
// skip this block
while (bioReader.HasLines)
{
bioReader.GoToNextLine();
if (0 == string.Compare(bioReader.Line, "end;", StringComparison.OrdinalIgnoreCase))
{
break;
}
}
break;
}
bioReader.GoToNextLine();
}
}
return(sequenceAlignment);
}
/// <summary>
/// Parses a single biological sequence alignment text from a stream.
/// </summary>
/// <param name="reader">Reader</param>
/// <returns>Sequence</returns>
private ISequenceAlignment ParseOne(StreamReader reader)
{
// no empty files allowed
if (line == null)
{
ReadNextLine(reader);
}
if (line == null)
{
throw new InvalidDataException(Properties.Resource.IONoTextToParse);
}
if (!line.StartsWith("CLUSTAL", StringComparison.OrdinalIgnoreCase))
{
throw new InvalidDataException(
string.Format(CultureInfo.CurrentCulture, Properties.Resource.INVALID_INPUT_FILE, this.Name));
}
ReadNextLine(reader); // Skip blank lines until we get to the first block.
// Now that we're at the first block, one or more blank lines are the block separators, which we'll need.
skipBlankLines = false;
var mapIdToSequence = new Dictionary <string, Tuple <ISequence, List <byte> > >();
IAlphabet alignmentAlphabet = null;
bool isFirstBlock = true;
bool inBlock = false;
var endOfBlockSymbols = new HashSet <char> {
'*', ' ', '.', '+', ':'
};
while (reader.Peek() != -1)
{
// Blank line or consensus line signals end of block.
if (String.IsNullOrEmpty(line) || line.ToCharArray().All(endOfBlockSymbols.Contains))
{
if (inBlock)
{
// Blank line signifies end of block
inBlock = false;
isFirstBlock = false;
}
}
else // It's not a blank or consensus line.
{
// It's a data line in a block.
// Lines begin with sequence id, then the sequence segment, and optionally a number, which we will ignore
string[] tokens = line.Split((char[])null, StringSplitOptions.RemoveEmptyEntries); // (char[])null uses whitespace delimiters
string id = tokens[0];
string data = tokens[1].ToUpperInvariant();
byte[] byteData = Encoding.UTF8.GetBytes(data);
Tuple <ISequence, List <byte> > sequenceTuple;
IAlphabet alphabet = Alphabet;
inBlock = true;
if (isFirstBlock)
{
if (null == alphabet)
{
alphabet = Alphabets.AutoDetectAlphabet(byteData, 0, byteData.Length, alphabet);
if (null == alphabet)
{
throw new InvalidDataException(string.Format(
CultureInfo.InvariantCulture,
Properties.Resource.InvalidSymbolInString,
data));
}
if (null == alignmentAlphabet)
{
alignmentAlphabet = alphabet;
}
else
{
if (alignmentAlphabet != alphabet)
{
throw new InvalidDataException(string.Format(
CultureInfo.CurrentCulture,
Properties.Resource.SequenceAlphabetMismatch));
}
}
}
sequenceTuple = new Tuple <ISequence, List <byte> >(
new Sequence(alphabet, "")
{
ID = id
},
new List <byte>());
sequenceTuple.Item2.AddRange(byteData);
mapIdToSequence.Add(id, sequenceTuple);
}
else
{
if (!mapIdToSequence.ContainsKey(id))
{
throw new InvalidDataException(string.Format(CultureInfo.CurrentCulture, Properties.Resource.ClustalUnknownSequence, id));
}
sequenceTuple = mapIdToSequence[id];
sequenceTuple.Item2.AddRange(byteData);
}
}
ReadNextLine(reader);
}
var sequenceAlignment = new SequenceAlignment();
var alignedSequence = new AlignedSequence();
sequenceAlignment.AlignedSequences.Add(alignedSequence);
foreach (var alignmentSequenceTuple in mapIdToSequence.Values)
{
alignedSequence.Sequences.Add(
new Sequence(alignmentSequenceTuple.Item1.Alphabet, alignmentSequenceTuple.Item2.ToArray())
{
ID = alignmentSequenceTuple.Item1.ID
});
}
return(sequenceAlignment);
}
/// <summary>
/// Parses a single biological sequence alignment text from a reader.
/// </summary>
/// <param name="reader">A reader for a biological sequence alignment text.</param>
/// <returns>The parsed ISequenceAlignment object.</returns>
public ISequenceAlignment ParseOne(TextReader reader)
{
string message = string.Empty;
if (reader == null)
{
throw new ArgumentNullException("reader");
}
if (line == null)
{
ReadNextLine(reader);
}
// no empty files allowed
if (line == null)
{
throw new InvalidDataException(Properties.Resource.IONoTextToParse);
}
// Parse first line
IList <string> tokens = line.Split((char[])null, StringSplitOptions.RemoveEmptyEntries);
if (2 != tokens.Count)
{
message = string.Format(CultureInfo.CurrentCulture, Properties.Resource.INVALID_INPUT_FILE, this.Name);
throw new InvalidDataException(message);
}
bool isFirstBlock = true;
int sequenceCount = 0;
int sequenceLength = 0;
IList <Tuple <Sequence, List <byte> > > data = new List <Tuple <Sequence, List <byte> > >();
string id = string.Empty;
string sequenceString = string.Empty;
Tuple <Sequence, List <byte> > sequence = null;
IAlphabet alignmentAlphabet = null;
sequenceCount = Int32.Parse(tokens[0], CultureInfo.InvariantCulture);
sequenceLength = Int32.Parse(tokens[1], CultureInfo.InvariantCulture);
ReadNextLine(reader); // Skip blank lines until we get to the first block.
// Now that we're at the first block, one or more blank lines are the block separators, which we'll need.
skipBlankLines = false;
while (reader.Peek() != -1)
{
if (string.IsNullOrWhiteSpace(line))
{
ReadNextLine(reader);
continue;
}
for (int index = 0; index < sequenceCount; index++)
{
if (isFirstBlock)
{
// First 10 characters are sequence ID, remaining is the first block of sequence
// Note that both may contain whitespace, and there may be no whitespace between them.
if (line.Length <= 10)
{
message = string.Format(CultureInfo.CurrentCulture, Properties.Resource.INVALID_INPUT_FILE, this.Name);
throw new Exception(message);
}
id = line.Substring(0, 10).Trim();
sequenceString = line.Substring(10).Replace(" ", "");
byte[] sequenceBytes = System.Text.ASCIIEncoding.ASCII.GetBytes(sequenceString);
IAlphabet alphabet = Alphabet;
if (null == alphabet)
{
alphabet = Alphabets.AutoDetectAlphabet(sequenceBytes, 0, sequenceBytes.Length, alphabet);
if (null == alphabet)
{
message = string.Format(
CultureInfo.InvariantCulture,
Properties.Resource.InvalidSymbolInString,
sequenceString);
throw new InvalidDataException(message);
}
else
{
if (null == alignmentAlphabet)
{
alignmentAlphabet = alphabet;
}
else
{
if (alignmentAlphabet != alphabet)
{
throw new InvalidDataException(Properties.Resource.SequenceAlphabetMismatch);
}
}
}
}
Tuple <Sequence, List <byte> > sequenceStore = new Tuple <Sequence, List <byte> >(
new Sequence(alphabet, string.Empty)
{
ID = id
},
new List <byte>());
sequenceStore.Item2.AddRange(sequenceBytes);
data.Add(sequenceStore);
}
else
{
sequence = data[index];
byte[] sequenceBytes = System.Text.ASCIIEncoding.ASCII.GetBytes(line.Replace(" ", ""));
sequence.Item2.AddRange(sequenceBytes);
}
ReadNextLine(reader);
}
// Reset the first block flag
isFirstBlock = false;
}
// Validate for the count of sequence
if (sequenceCount != data.Count)
{
throw new InvalidDataException(Properties.Resource.SequenceCountMismatch);
}
SequenceAlignment sequenceAlignment = new SequenceAlignment();
sequenceAlignment.AlignedSequences.Add(new AlignedSequence());
foreach (var dataSequence in data)
{
// Validate for the count of sequence
if (sequenceLength != dataSequence.Item2.Count)
{
throw new InvalidDataException(Properties.Resource.SequenceLengthMismatch);
}
sequenceAlignment.AlignedSequences[0].Sequences.Add(
new Sequence(dataSequence.Item1.Alphabet, dataSequence.Item2.ToArray())
{
ID = dataSequence.Item1.ID
});
}
return(sequenceAlignment);
}
/// <summary>
/// Parses a single Phylip text from a reader into a sequence.
/// 1. First link has Count of Taxa and length of each sequence
/// 2. Sequences
/// a. First ten character are ID
/// b. Sequence itself
/// </summary>
/// <param name="bioReader">A reader for a biological sequence text.</param>
/// <param name="isReadOnly">
/// Flag to indicate whether the resulting sequence alignment should be in readonly mode or not.
/// If this flag is set to true then the resulting sequence's isReadOnly property
/// will be set to true, otherwise it will be set to false.</param>
/// <returns>A new Sequence Alignment instance containing parsed data.</returns>
protected ISequenceAlignment ParseOneWithSpecificFormat(BioTextReader bioReader, bool isReadOnly)
{
if (bioReader == null)
{
throw new ArgumentNullException("bioReader");
}
string message = string.Empty;
// Parse first line
IList <string> tokens = GetTokens(bioReader.Line);
if (2 != tokens.Count)
{
message = string.Format(CultureInfo.CurrentCulture, Resource.INVAILD_INPUT_FILE, this.Name);
throw new InvalidDataException(message);
}
bool isFirstBlock = true;
int sequenceCount = 0;
int sequenceLength = 0;
IList <Sequence> data = new List <Sequence>();
string id = string.Empty;
string sequenceString = string.Empty;
Sequence sequence = null;
IAlphabet alignmentAlphabet = null;
sequenceCount = Int32.Parse(tokens[0], CultureInfo.InvariantCulture);
sequenceLength = Int32.Parse(tokens[1], CultureInfo.InvariantCulture);
bioReader.GoToNextLine(); // Skip blank lines until we get to the first block.
// Now that we're at the first block, one or more blank lines are the block separators, which we'll need.
bioReader.SkipBlankLines = false;
while (bioReader.HasLines)
{
if (string.IsNullOrEmpty(bioReader.Line.Trim()))
{
bioReader.GoToNextLine();
continue;
}
for (int index = 0; index < sequenceCount; index++)
{
if (isFirstBlock)
{
tokens = GetTokens(bioReader.Line);
if (1 == tokens.Count)
{
id = tokens[0].Substring(0, 10);
sequenceString = tokens[0].Substring(10);
}
else
{
id = tokens[0];
sequenceString = tokens[1];
}
IAlphabet alphabet = Alphabet;
if (null == alphabet)
{
alphabet = _basicParser.IdentifyAlphabet(alphabet, sequenceString);
if (null == alphabet)
{
message = string.Format(
CultureInfo.InvariantCulture,
Resource.InvalidSymbolInString,
sequenceString);
throw new InvalidDataException(message);
}
else
{
if (null == alignmentAlphabet)
{
alignmentAlphabet = alphabet;
}
else
{
if (alignmentAlphabet != alphabet)
{
message = Properties.Resource.SequenceAlphabetMismatch;
throw new InvalidDataException(message);
}
}
}
}
if (Encoding == null)
{
sequence = new Sequence(alphabet, sequenceString);
}
else
{
sequence = new Sequence(alphabet, Encoding, sequenceString);
}
sequence.ID = id;
sequence.IsReadOnly = false;
data.Add(sequence);
}
else
{
sequence = data[index];
sequence.InsertRange(sequence.Count, bioReader.Line.Trim());
}
bioReader.GoToNextLine();
}
// Reset the first block flag
isFirstBlock = false;
}
// Validate for the count of sequence
if (sequenceCount != data.Count)
{
throw new InvalidDataException(Properties.Resource.SequenceCountMismatch);
}
SequenceAlignment sequenceAlignment = new SequenceAlignment();
sequenceAlignment.AlignedSequences.Add(new AlignedSequence());
foreach (Sequence dataSequence in data)
{
dataSequence.IsReadOnly = isReadOnly;
// Validate for the count of sequence
if (sequenceLength != dataSequence.Count)
{
throw new InvalidDataException(Properties.Resource.SequenceLengthMismatch);
}
sequenceAlignment.AlignedSequences[0].Sequences.Add(dataSequence);
}
return(sequenceAlignment);
}
/// <summary>
/// Parses a single ClustalW text from a reader into a sequence.
/// </summary>
/// <param name="bioReader">A reader for a biological sequence text.</param>
/// <param name="isReadOnly">
/// Flag to indicate whether the resulting sequence alignment should be in readonly mode or not.
/// If this flag is set to true then the resulting sequence's isReadOnly property
/// will be set to true, otherwise it will be set to false.</param>
/// <returns>A new Sequence Alignment instance containing parsed data.</returns>
protected ISequenceAlignment ParseOneWithSpecificFormat(BioTextReader bioReader, bool isReadOnly)
{
if (bioReader == null)
{
throw new ArgumentNullException("bioReader");
}
string message = string.Empty;
if (!bioReader.Line.StartsWith("CLUSTAL", StringComparison.OrdinalIgnoreCase))
{
message = string.Format(CultureInfo.CurrentCulture, Resource.INVAILD_INPUT_FILE, this.Name);
throw new InvalidDataException(message);
}
bioReader.GoToNextLine(); // Skip blank lines until we get to the first block.
// Now that we're at the first block, one or more blank lines are the block separators, which we'll need.
bioReader.SkipBlankLines = false;
Dictionary <string, ISequence> mapIdToSequence = new Dictionary <string, ISequence>();
IAlphabet alignmentAlphabet = null;
bool isFirstBlock = true;
bool inBlock = false;
while (bioReader.HasLines)
{
// Blank line or consensus line signals end of block.
if (String.IsNullOrEmpty(bioReader.Line) ||
Helper.ContainsOnly(bioReader.Line, '*', ' ', '.', '+', ':'))
{
if (inBlock)
{
// Blank line signifies end of block
inBlock = false;
isFirstBlock = false;
}
}
else // It's not a blank or consensus line.
{
// It's a data line in a block.
// Lines begin with sequence id, then the sequence segment, and optionally a number, which we will ignore
string[] tokens = bioReader.Line.Split((char[])null, StringSplitOptions.RemoveEmptyEntries); // (char[])null uses whitespace delimiters
string id = tokens[0];
string data = tokens[1].ToUpper(CultureInfo.InvariantCulture);
Sequence sequence = null;
IAlphabet alphabet = Alphabet;
inBlock = true;
if (isFirstBlock)
{
if (null == alphabet)
{
alphabet = _basicParser.IdentifyAlphabet(alphabet, data);
if (null == alphabet)
{
message = string.Format(
CultureInfo.InvariantCulture,
Resource.InvalidSymbolInString,
data);
throw new InvalidDataException(message);
}
else
{
if (null == alignmentAlphabet)
{
alignmentAlphabet = alphabet;
}
else
{
if (alignmentAlphabet != alphabet)
{
message = string.Format(
CultureInfo.CurrentCulture,
Properties.Resource.SequenceAlphabetMismatch);
throw new InvalidDataException(message);
}
}
}
}
if (Encoding == null)
{
sequence = new Sequence(alphabet, data);
}
else
{
sequence = new Sequence(alphabet, Encoding, data);
}
sequence.ID = id;
sequence.IsReadOnly = false;
mapIdToSequence.Add(id, sequence);
}
else
{
if (!mapIdToSequence.ContainsKey(id))
{
message = string.Format(CultureInfo.CurrentCulture, Properties.Resource.ClustalUnknownSequence, id);
throw new InvalidDataException(message);
}
sequence = (Sequence)mapIdToSequence[id];
sequence.InsertRange(sequence.Count, data);
}
}
bioReader.GoToNextLine();
}
SequenceAlignment sequenceAlignment = new SequenceAlignment();
sequenceAlignment.AlignedSequences.Add(new AlignedSequence());
foreach (Sequence alignmentSequence in mapIdToSequence.Values)
{
alignmentSequence.IsReadOnly = isReadOnly;
sequenceAlignment.AlignedSequences[0].Sequences.Add(alignmentSequence);
}
return(sequenceAlignment);
}
/// <summary>
/// Parses a single biological sequence alignment text from a reader.
/// </summary>
/// <param name="reader">A reader for a biological sequence alignment text.</param>
/// <returns>The parsed ISequenceAlignment object.</returns>
ISequenceAlignment ParseOne(TextReader reader)
{
ReadNextLine(reader);
if (line == null)
{
throw new Exception(Properties.Resource.INVALID_INPUT_FILE);
}
this.ParseHeader(reader);
var alignedSequence = new AlignedSequence();
IList <string> ids = null;
bool isInBlock = true;
if (this.line.StartsWith("begin", StringComparison.OrdinalIgnoreCase))
{
while (this.line != null && isInBlock)
{
if (string.IsNullOrEmpty(this.line.Trim()))
{
this.ReadNextLine(reader);
continue;
}
string blockName = GetTokens(this.line)[1];
switch (blockName.ToUpperInvariant())
{
case "TAXA":
case "TAXA;":
// This block contains the count of sequence & title of each sequence
ids = this.ParseTaxaBlock(reader);
break;
case "CHARACTERS":
case "CHARACTERS;":
// Block contains sequences
Dictionary <string, string> dataSet = this.ParseCharacterBlock(reader, ids);
IAlphabet alignmentAlphabet = null;
foreach (string id in ids)
{
IAlphabet alphabet = this.Alphabet;
string data = dataSet[id];
if (null == alphabet)
{
byte[] dataArray = data.ToByteArray();
alphabet = Alphabets.AutoDetectAlphabet(dataArray, 0, dataArray.Length, null);
if (null == alphabet)
{
throw new InvalidDataException(string.Format(
CultureInfo.InvariantCulture,
Properties.Resource.InvalidSymbolInString,
data));
}
if (null == alignmentAlphabet)
{
alignmentAlphabet = alphabet;
}
else
{
if (alignmentAlphabet != alphabet)
{
throw new InvalidDataException(string.Format(
CultureInfo.InvariantCulture,
Properties.Resource.SequenceAlphabetMismatch));
}
}
}
alignedSequence.Sequences.Add(new Sequence(alphabet, data)
{
ID = id
});
}
break;
case "END":
case "END;":
// Have reached the end of block
isInBlock = false;
break;
default:
// skip this block
while (this.line != null)
{
this.ReadNextLine(reader);
if (0 == string.Compare(this.line, "end;", StringComparison.OrdinalIgnoreCase))
{
break;
}
}
break;
}
this.ReadNextLine(reader);
}
}
ISequenceAlignment sequenceAlignment = new SequenceAlignment();
sequenceAlignment.AlignedSequences.Add(alignedSequence);
return(sequenceAlignment);
}
/// <summary>
/// Parses a single Phylip text from a reader into a sequence.
/// 1. First link has Count of Taxa and length of each sequence
/// 2. Sequences
/// a. First ten character are ID
/// b. Sequence itself
/// </summary>
/// <param name="mbfReader">A reader for a biological sequence text.</param>
/// <param name="isReadOnly">
/// Flag to indicate whether the resulting sequence alignment should be in readonly mode or not.
/// If this flag is set to true then the resulting sequence's isReadOnly property
/// will be set to true, otherwise it will be set to false.</param>
/// <returns>A new Sequence Alignment instance containing parsed data.</returns>
protected ISequenceAlignment ParseOneWithSpecificFormat(MBFTextReader mbfReader, bool isReadOnly)
{
if (mbfReader == null)
{
throw new ArgumentNullException("mbfReader");
}
string message = string.Empty;
// Parse first line
IList <string> tokens = mbfReader.Line.Split((char[])null, StringSplitOptions.RemoveEmptyEntries);
if (2 != tokens.Count)
{
message = string.Format(CultureInfo.CurrentCulture, Resource.INVALID_INPUT_FILE, this.Name);
throw new InvalidDataException(message);
}
bool isFirstBlock = true;
int sequenceCount = 0;
int sequenceLength = 0;
IList <Sequence> data = new List <Sequence>();
string id = string.Empty;
string sequenceString = string.Empty;
Sequence sequence = null;
IAlphabet alignmentAlphabet = null;
sequenceCount = Int32.Parse(tokens[0], CultureInfo.InvariantCulture);
sequenceLength = Int32.Parse(tokens[1], CultureInfo.InvariantCulture);
mbfReader.GoToNextLine(); // Skip blank lines until we get to the first block.
// Now that we're at the first block, one or more blank lines are the block separators, which we'll need.
mbfReader.SkipBlankLines = false;
while (mbfReader.HasLines)
{
if (string.IsNullOrEmpty(mbfReader.Line.Trim()))
{
mbfReader.GoToNextLine();
continue;
}
for (int index = 0; index < sequenceCount; index++)
{
if (isFirstBlock)
{
// First 10 characters are sequence ID, remaining is the first block of sequence
// Note that both may contain whitespace, and there may be no whitespace between them.
if (mbfReader.Line.Length <= 10)
{
message = string.Format(CultureInfo.CurrentCulture, Resource.INVALID_INPUT_FILE, this.Name);
throw new Exception(message);
}
id = mbfReader.Line.Substring(0, 10).Trim();
sequenceString = Util.Helper.StringRemoveWhitespace(mbfReader.Line.Substring(10));
IAlphabet alphabet = Alphabet;
if (null == alphabet)
{
alphabet = _basicParser.IdentifyAlphabet(alphabet, sequenceString);
if (null == alphabet)
{
message = string.Format(
CultureInfo.InvariantCulture,
Resource.InvalidSymbolInString,
sequenceString);
throw new InvalidDataException(message);
}
else
{
if (null == alignmentAlphabet)
{
alignmentAlphabet = alphabet;
}
else
{
if (alignmentAlphabet != alphabet)
{
message = Properties.Resource.SequenceAlphabetMismatch;
throw new InvalidDataException(message);
}
}
}
}
if (Encoding == null)
{
sequence = new Sequence(alphabet, sequenceString);
}
else
{
sequence = new Sequence(alphabet, Encoding, sequenceString);
}
sequence.ID = id;
sequence.IsReadOnly = false;
data.Add(sequence);
}
else
{
sequence = data[index];
sequence.InsertRange(sequence.Count, Util.Helper.StringRemoveWhitespace(mbfReader.Line));
}
mbfReader.GoToNextLine();
}
// Reset the first block flag
isFirstBlock = false;
}
// Validate for the count of sequence
if (sequenceCount != data.Count)
{
throw new InvalidDataException(Properties.Resource.SequenceCountMismatch);
}
SequenceAlignment sequenceAlignment = new SequenceAlignment();
sequenceAlignment.AlignedSequences.Add(new AlignedSequence());
foreach (Sequence dataSequence in data)
{
dataSequence.IsReadOnly = isReadOnly;
// Validate for the count of sequence
if (sequenceLength != dataSequence.Count)
{
throw new InvalidDataException(Properties.Resource.SequenceLengthMismatch);
}
sequenceAlignment.AlignedSequences[0].Sequences.Add(dataSequence);
}
return(sequenceAlignment);
}
/// <summary>
/// Parses a single biological sequence alignment text from a reader.
/// </summary>
/// <param name="reader">A reader for a biological sequence alignment text.</param>
/// <returns>The parsed ISequenceAlignment object.</returns>
public ISequenceAlignment ParseOne(TextReader reader)
{
if (reader == null)
{
throw new ArgumentNullException("reader");
}
ReadNextLine(reader);
if (line == null)
{
string message = Properties.Resource.INVALID_INPUT_FILE;
Trace.Report(message);
throw new FileFormatException(message);
}
else
{
ParseHeader(reader);
string message = string.Empty;
ISequenceAlignment sequenceAlignment = new SequenceAlignment();
sequenceAlignment.AlignedSequences.Add(new AlignedSequence());
IList <string> ids = null;
bool isInBlock = true;
if (line.StartsWith("begin", StringComparison.OrdinalIgnoreCase))
{
while (line != null && isInBlock)
{
if (string.IsNullOrEmpty(line.Trim()))
{
ReadNextLine(reader);
continue;
}
string blockName = GetTokens(line)[1];
switch (blockName.ToUpper(CultureInfo.InvariantCulture))
{
case "TAXA":
case "TAXA;":
// This block contains the count of sequence & title of each sequence
ids = (IList <string>)ParseTaxaBlock(reader);
break;
case "CHARACTERS":
case "CHARACTERS;":
// Block contains sequences
Dictionary <string, string> dataSet = ParseCharacterBlock(reader, ids);
IAlphabet alignmentAlphabet = null;
string data = string.Empty;
foreach (string ID in ids)
{
IAlphabet alphabet = Alphabet;
Sequence sequence = null;
data = dataSet[ID];
if (null == alphabet)
{
byte[] dataArray = data.Select(a => (byte)a).ToArray();
alphabet = Alphabets.AutoDetectAlphabet(dataArray, 0, dataArray.Length, null);
if (null == alphabet)
{
message = string.Format(
CultureInfo.InvariantCulture,
Properties.Resource.InvalidSymbolInString,
data);
throw new InvalidDataException(message);
}
else
{
if (null == alignmentAlphabet)
{
alignmentAlphabet = alphabet;
}
else
{
if (alignmentAlphabet != alphabet)
{
message = string.Format(
CultureInfo.InvariantCulture,
Properties.Resource.SequenceAlphabetMismatch);
throw new InvalidDataException(message);
}
}
}
}
sequence = new Sequence(alphabet, data);
sequence.ID = ID;
sequenceAlignment.AlignedSequences[0].Sequences.Add(sequence);
}
break;
case "END":
case "END;":
// Have reached the end of block
isInBlock = false;
break;
default:
// skip this block
while (line != null)
{
ReadNextLine(reader);
if (0 == string.Compare(line, "end;", StringComparison.OrdinalIgnoreCase))
{
break;
}
}
break;
}
ReadNextLine(reader);
}
}
return(sequenceAlignment);
}
}