public GeneFragment CreateGeneFragment()
{
GeneFragment geneFragment = new GeneFragment()
{
OligoSequence = "TTT",
OverlappingLength = 1,
};
return(GetOrCreate(() => geneFragment));
}
public void UpdateGeneFragment(GeneFragmentItemViewModel geneFragmentItemViewModel)
{
Gene gene = unitOfWork.GetById <Gene>(geneFragmentItemViewModel.GeneId);
GeneFragment geneFragment = unitOfWork.GetById <GeneFragment>(geneFragmentItemViewModel.Id);
geneFragment.OligoSequence = geneFragmentItemViewModel.OligoSequence;
geneFragment.OligoLength = geneFragmentItemViewModel.OligoSequence.Length;
geneFragment.OverlappingLength = geneFragmentItemViewModel.OverlappingLength;
geneFragment.Tm = CalculateTm(geneFragment.OligoSequence, gene.KPlusConcentration, gene.DMSO, geneFragment.OverlappingLength);
geneFragment.Gene = gene;
unitOfWork.InsertOrUpdate(geneFragment);
unitOfWork.Commit();
}
private GeneFragment CreateGeneFragment(string geneId, int fragmentNumber, string geneFragmentSequence,
int overlappingLength, float kPlusConcentration, float dmso)
{
var geneFragment = new GeneFragment()
{
FragmentNumber = fragmentNumber,
OligoSequence = geneFragmentSequence,
OligoLength = geneFragmentSequence.Length,
OverlappingLength = overlappingLength,
GeneId = geneId,
CreatedAt = DateTimeOffset.UtcNow
//Gene = gene,
};
geneFragment.Tm = CalculateTm(geneFragmentSequence, kPlusConcentration, dmso, overlappingLength);
return(geneFragment);
}
