private string checkPCRResult(PCRColumnData data, string testItemName, bool isSingle, Tubetype tubeType)
{
string rox = null;
string hex = null;
string fam = null;
if (data is ABIPCRColumnData)
{
ABIPCRColumnData aiData = (ABIPCRColumnData)data;
if ("ROX" == aiData.Detector)
{
rox = aiData.Ct;
}
else if ("FAM" == aiData.Detector)
{
fam = aiData.Ct;
}
else if ("HEX" == aiData.Detector)
{
hex = aiData.Ct;
}
if (aiData.DataList != null)
{
foreach (ABIPCRColumnData subAiData in aiData.DataList)
{
if ("ROX" == subAiData.Detector)
{
rox = subAiData.Ct;
}
else if ("FAM" == subAiData.Detector)
{
fam = subAiData.Ct;
}
else if ("HEX" == subAiData.Detector)
{
hex = subAiData.Ct;
}
}
}
}
else if (data is BIORADPCRColumnData)
{
BIORADPCRColumnData bioData = (BIORADPCRColumnData)data;
if ("FAM" == bioData.Fluor)
{
fam = bioData.Cq;
}
else if ("HEX" == bioData.Fluor)
{
hex = bioData.Cq;
}
else if ("ROX" == bioData.Fluor)
{
rox = bioData.Cq;
}
if (bioData.DataList != null)
{
foreach (BIORADPCRColumnData subBioData in bioData.DataList)
{
if ("FAM" == subBioData.Fluor)
{
fam = subBioData.Cq;
}
else if ("HEX" == subBioData.Fluor)
{
hex = subBioData.Cq;
}
else if ("ROX" == subBioData.Fluor)
{
rox = subBioData.Cq;
}
}
}
}
else if (data is StratagenePCRColumnData)
{
StratagenePCRColumnData sData = (StratagenePCRColumnData)data;
if ("FAM" == sData.Dye)
{
fam = sData.Ct;
}
else if ("HEX" == sData.Dye)
{
hex = sData.Ct;
}
else if ("ROX" == sData.Dye)
{
rox = sData.Ct;
}
if (sData.DataList != null)
{
foreach (StratagenePCRColumnData subSata in sData.DataList)
{
if ("FAM" == subSata.Dye)
{
fam = subSata.Ct;
}
else if ("HEX" == subSata.Dye)
{
hex = subSata.Ct;
}
else if ("ROX" == subSata.Dye)
{
rox = subSata.Ct;
}
}
}
}
if (!string.IsNullOrEmpty(fam) && (fam == "Undetermined" || fam == "No Ct" || fam == "N/A"))
{
fam = "45";
}
if (!string.IsNullOrEmpty(hex) && (hex == "Undetermined" || hex == "No Ct" || hex == "N/A"))
{
hex = "45";
}
if (!string.IsNullOrEmpty(rox) && (rox == "Undetermined" || rox == "No Ct" || rox == "N/A"))
{
rox = "45";
}
float outNumber = 0;
//阴性对照
if (tubeType == Tubetype.NegativeControl)
{
if (testItemName == "HBV")
{
if (!string.IsNullOrEmpty(hex) && float.TryParse(hex, out outNumber))
{
float hexNumber = float.Parse(hex);
if (hexNumber > 37 && !string.IsNullOrEmpty(rox) && float.TryParse(rox, out outNumber) && float.Parse(rox) < 36)
{
return(PCRTest.NegativeResult);
}
}
return(PCRTest.InvalidResult);
}
else if (testItemName == "HCV")
{
if (!string.IsNullOrEmpty(fam) && float.TryParse(fam, out outNumber))
{
float famNumber = float.Parse(fam);
if (famNumber > 42 && !string.IsNullOrEmpty(rox) && float.TryParse(rox, out outNumber) && float.Parse(rox) < 36)
{
return(PCRTest.NegativeResult);
}
}
return(PCRTest.InvalidResult);
}
else if (testItemName == "HIV")
{
if (!string.IsNullOrEmpty(fam) && float.TryParse(fam, out outNumber))
{
float famNumber = float.Parse(fam);
if (famNumber > 42 && !string.IsNullOrEmpty(rox) && float.TryParse(rox, out outNumber) && float.Parse(rox) < 36)
{
return(PCRTest.NegativeResult);
}
}
return(PCRTest.InvalidResult);
}
else
{
return(PCRTest.NoResult);
}
}
else if (tubeType == Tubetype.PositiveControl)
{
if (testItemName == "HBV")
{
if (!string.IsNullOrEmpty(hex) && float.TryParse(hex, out outNumber))
{
float hexNumber = float.Parse(hex);
if (hexNumber <= 33 && !string.IsNullOrEmpty(rox) && float.TryParse(rox, out outNumber) && float.Parse(rox) < 36)
{
return(PCRTest.PositiveResult);
}
}
return(PCRTest.InvalidResult);
}
else if (testItemName == "HCV")
{
if (!string.IsNullOrEmpty(fam) && float.TryParse(fam, out outNumber))
{
float famNumber = float.Parse(fam);
if (famNumber <= 37 && !string.IsNullOrEmpty(rox) && float.TryParse(rox, out outNumber) && float.Parse(rox) < 36)
{
return(PCRTest.PositiveResult);
}
}
return(PCRTest.InvalidResult);
}
else if (testItemName == "HIV")
{
if (!string.IsNullOrEmpty(fam) && float.TryParse(fam, out outNumber))
{
float famNumber = float.Parse(fam);
if (famNumber <= 37 && !string.IsNullOrEmpty(rox) && float.TryParse(rox, out outNumber) && float.Parse(rox) < 36)
{
return(PCRTest.PositiveResult);
}
}
return(PCRTest.InvalidResult);
}
else
{
return(PCRTest.NoResult);
}
}
float HBVValue = 0;
float HCVValue = 0;
float HIVValue = 0;
if (isSingle)
{
HBVValue = 35;
HCVValue = 42;
HIVValue = 40;
}
else
{
HBVValue = 37;
HCVValue = 42;
HIVValue = 42;
}
if (testItemName == "HBV")
{
if (!string.IsNullOrEmpty(hex) && float.TryParse(hex, out outNumber))
{
float hexNumber = float.Parse(hex);
if (hexNumber > HBVValue && !string.IsNullOrEmpty(rox) && float.TryParse(rox, out outNumber) && float.Parse(rox) < 36)
{
return(PCRTest.NegativeResult);
}
else if (hexNumber <= HBVValue)
{
return(PCRTest.PositiveResult);
}
else if (hexNumber > HBVValue && !string.IsNullOrEmpty(rox) && float.TryParse(rox, out outNumber) && float.Parse(rox) >= 36)
{
return(PCRTest.InvalidResult);
}
else
{
return(PCRTest.NoResult);
}
}
else
{
return(PCRTest.NoResult);
}
}
else if (testItemName == "HCV")
{
if (!string.IsNullOrEmpty(fam) && float.TryParse(fam, out outNumber))
{
float famNumber = float.Parse(fam);
if (famNumber > HCVValue && !string.IsNullOrEmpty(rox) && float.TryParse(rox, out outNumber) && float.Parse(rox) < 36)
{
return(PCRTest.NegativeResult);
}
else if (famNumber <= HCVValue)
{
return(PCRTest.PositiveResult);
}
else if (famNumber > HCVValue && !string.IsNullOrEmpty(rox) && float.TryParse(rox, out outNumber) && float.Parse(rox) >= 36)
{
return(PCRTest.InvalidResult);
}
else
{
return(PCRTest.NoResult);
}
}
else
{
return(PCRTest.NoResult);
}
}
else if (testItemName == "HIV")
{
if (!string.IsNullOrEmpty(fam) && float.TryParse(fam, out outNumber))
{
float famNumber = float.Parse(fam);
if (famNumber > HIVValue && !string.IsNullOrEmpty(rox) && float.TryParse(rox, out outNumber) && float.Parse(rox) < 36)
{
return(PCRTest.NegativeResult);
}
else if (famNumber <= HIVValue)
{
return(PCRTest.PositiveResult);
}
else if (famNumber > HIVValue && !string.IsNullOrEmpty(rox) && float.TryParse(rox, out outNumber) && float.Parse(rox) >= 36)
{
return(PCRTest.InvalidResult);
}
else
{
return(PCRTest.NoResult);
}
}
else
{
return(PCRTest.NoResult);
}
}
else
{
return(PCRTest.NoResult);
}
}
private bool processStratageneFile(string fileName, Guid rotationId, Plate plate, out bool isRightPositionNumber)
{
isRightPositionNumber = true;
OleDbConnectionStringBuilder connectionStringBuilder = new OleDbConnectionStringBuilder();
connectionStringBuilder.Provider = "Microsoft.Jet.OLEDB.4.0";
connectionStringBuilder.DataSource = fileName;
connectionStringBuilder.Add("Mode", "Read");
connectionStringBuilder.Add("Extended Properties", "Excel 8.0;IMEX=1;TypeGuessRows=0;ImportMixedTypes=Text");
bool isRightFormat = false;
bool hasWellColumn = false;
bool hasDyeColumn = false;
bool hasCtColumn = false;
using (OleDbConnection con = new OleDbConnection(connectionStringBuilder.ToString()))
{
con.Open();
DataTable dtSchema = con.GetOleDbSchemaTable(OleDbSchemaGuid.Tables, new object[] { null, null, null, "TABLE" });
string Sheet1 = dtSchema.Rows[0].Field <string>("TABLE_NAME");
DataTable sheetColumns = con.GetOleDbSchemaTable(OleDbSchemaGuid.Columns, new object[] { null, null, Sheet1, null });
foreach (DataRow row in sheetColumns.Rows)
{
string strColumnName = row["COLUMN_NAME"].ToString();
if (strColumnName == "Well")
{
hasWellColumn = true;
}
else if (strColumnName == "Dye")
{
hasDyeColumn = true;
}
if (strColumnName == "Ct (dR)")
{
hasCtColumn = true;
}
}
if (hasWellColumn && hasDyeColumn && hasCtColumn)
{
isRightFormat = true;
}
if (!isRightFormat)
{
return(isRightFormat);
}
OleDbDataAdapter da = new OleDbDataAdapter("select [Well],[Dye],[Ct (dR)] from [" + Sheet1 + "]", con);
DataTable dt = new DataTable();
da.Fill(dt);
Dictionary <int, DataRow> dc = controller.GetPCRPositionsByPlateID(plate.PlateID, currentExperimentId);
System.Collections.Generic.Dictionary <string, StratagenePCRColumnData> dataList = new System.Collections.Generic.Dictionary <string, StratagenePCRColumnData>();
foreach (DataRow row in dt.Rows)
{
StratagenePCRColumnData sData = new StratagenePCRColumnData()
{
Well = row[0].ToString(), Dye = row[1].ToString(), Ct = row[2].ToString()
};
sData.XmlContent = "<Well>" + sData.Well + "</Well><Dye>" + sData.Dye + "</Dye><Ct>" + sData.Ct + "</Ct>";
if (!dataList.ContainsKey(sData.Well))
{
dataList.Add(sData.Well, sData);
}
else
{
StratagenePCRColumnData _sData = (StratagenePCRColumnData)dataList[sData.Well];
if (_sData.DataList == null)
{
_sData.DataList = new List <PCRColumnData>();
_sData.DataList.Add(sData);
}
else
{
_sData.DataList.Add(sData);
}
}
}
if (dc.Count != dataList.Count)
{
isRightPositionNumber = false;
if (!isRightPositionNumber)
{
MessageBoxResult selectResult = MessageBox.Show("PCR仪检测结果和混样数不一致，请检查操作是否正确？“是”将按现有规则导入", "系统提示", MessageBoxButton.YesNo);
if (selectResult == MessageBoxResult.No)
{
return(isRightFormat);
}
else
{
isRightPositionNumber = true;
}
}
}
foreach (StratagenePCRColumnData sData in dataList.Values)
{
PCRTestResult info = new PCRTestResult();
info.ItemID = WanTaiObjectService.NewSequentialGuid();
info.RotationID = rotationId;
info.Position = controller.ChangeCharacterToPositionNumber(sData.Well);
info.CreateTime = DateTime.Now;
//calculate
if (dc.ContainsKey(info.Position))
{
DataRow dcrow = dc[info.Position];
bool isSingle = (int)dcrow["TubeNumber"] == 1 ? true : false;
Tubetype tubeType = (Tubetype)((short)dcrow["TubeType"]);
info.Result = checkPCRResult(sData, dcrow["TestName"].ToString(), isSingle, tubeType);
}
info.ExperimentID = currentExperimentId;
if (plate.BarCode != null)
{
info.BarCode = plate.BarCode;
}
info.PlateID = plate.PlateID;
string xmlContent = sData.XmlContent;
if (sData.DataList != null && sData.DataList.Count > 0)
{
foreach (StratagenePCRColumnData data in sData.DataList)
{
xmlContent = xmlContent + data.XmlContent;
}
}
info.PCRContent = "<PCRContent>" + xmlContent + "</PCRContent>";
pcrResultList.Add(info);
}
}
return(isRightFormat);
}
