private void waveformPlotData_dataAcquired(object sender)
{
EventPlotData pd = (EventPlotData)sender;
if (spkWfmGraph.Visible && !checkBox_freeze.Checked)
{
int maxWaveforms = pd.MaxWaveforms;
List <PlotSpikeWaveform> wfms = pd.read();
for (int i = 0; i < wfms.Count; ++i)
{
// What channel are we on
int channel = wfms[i].channel;
// Correct channel mappings
if (Properties.Settings.Default.ChannelMapping == "invitro")
{
channel = (MEAChannelMappings.ch2rcPreMapped[channel, 0] - 1) * 8 + MEAChannelMappings.ch2rcPreMapped[channel, 1] - 1;
}
// Plot the spikes
if (wfms[i].unit == null)
{
spkWfmGraph.plotY(wfms[i].waveform, pd.horizontalOffset(channel), 1, NRSnipBrainbow, channel,
numSpkWfms[channel]++ + channel * maxWaveforms);
}
else
{
spkWfmGraph.plotY(wfms[i].waveform, pd.horizontalOffset(channel), 1, NRUnitBrainbow[(int)wfms[i].unit], channel,
numSpkWfms[channel]++ + channel * maxWaveforms);
}
// Tally number of waveforms being displayed for each channel
numSpkWfms[channel] %= maxWaveforms;
}
spkWfmGraph.Invalidate();
}
else
{
pd.skipRead();
}
}
// Method to set up the recording side of neurorighter
private bool NRAcquisitionSetup()
{
lock (this)
{
if (!taskRunning)
{
try
{
this.Cursor = Cursors.WaitCursor;
if (switch_record.Value)
{
// Create file name
if (filenameBase == null) //user hasn't specified a file
button_BrowseOutputFile_Click(null, null); //call file selection routine
if (filenameBase == null) //this happens if the user pressed cancel for the dialog
{
MessageBox.Show("An output file must be selected before recording."); //display an error message
this.Cursor = Cursors.Default;
return true;
}
// If the user is just doing repeated recordings
if (checkbox_repeatRecord.Checked || Properties.Settings.Default.useFidTimeStamp)
{
DateTime nowDate = DateTime.Now;//Get current time (local to computer);
string datePrefix = nowDate.ToString("'-'yyyy'-'MM'-'dd'-'HH'-'mm'-'ss");
filenameBase = originalNameBase + datePrefix;
}
// Look for old files with same name
string[] matchFiles;
try
{
matchFiles = Directory.GetFiles(currentSaveDir, currentSaveFile + "*");
}
catch
{
matchFiles = new string[0];
}
if (matchFiles.Length > 0)
{
DialogResult dr = MessageBox.Show("File " + filenameBase + " exists. Overwrite?",
"NeuroRighter Warning", MessageBoxButtons.YesNoCancel, MessageBoxIcon.Warning);
if (dr == DialogResult.No)
button_BrowseOutputFile_Click(null, null); //call file selection routine
else if (dr == DialogResult.Cancel)
{
this.Cursor = Cursors.Default;
return true;
}
}
// Set file base name + number of channels
recordingSettings.SetFID(filenameBase);
recordingSettings.SetNumElectrodes(numChannels);
}
// Find out how many devs and channels/dev we are going to need
int numDevices = (numChannels > 32 ? Properties.Settings.Default.AnalogInDevice.Count : 1);
numChannelsPerDev = (numChannels < 32 ? numChannels : 32);
// Set spike buffer lengths
spikeBufferLength = Convert.ToInt32(Properties.Settings.Default.ADCPollingPeriodSec * Properties.Settings.Default.RawSampleFrequency);
lfpBufferLength = Convert.ToInt32(Properties.Settings.Default.ADCPollingPeriodSec * Properties.Settings.Default.LFPSampleFrequency);
// Create spike aquisition task list
spikeTask = new List<Task>(numDevices);
Properties.Settings.Default.numSpikeTasks = numDevices;
NRAIChannelCollection spikeAqSet = new NRAIChannelCollection(numDevices, numChannelsPerDev);
spikeAqSet.SetupSpikeCollection(ref spikeTask);
// Check audio and video properties
if (Properties.Settings.Default.UseSingleChannelPlayback)
spikeOutTask = new Task("spikeOutTask"); //For audio output
if (checkBox_video.Checked) //NB: This can't be checked unless video is enabled (no need to check properties)
triggerTask = new Task("triggerTask");
// Set MUA sample rate
double muaSamplingRate = spikeSamplingRate / MUA_DOWNSAMPLE_FACTOR;
//Add LFP channels, if configured
if (Properties.Settings.Default.SeparateLFPBoard && Properties.Settings.Default.UseLFPs)
{
lfpTask = new Task("lfpTask");
for (int i = 0; i < Properties.Settings.Default.NumChannels; ++i)
lfpTask.AIChannels.CreateVoltageChannel(Properties.Settings.Default.LFPDevice + "/ai" + i.ToString(), "",
AITerminalConfiguration.Nrse, -10.0, 10.0, AIVoltageUnits.Volts);
setGain(lfpTask, Properties.Settings.Default.LFPgain);
lfpTask.Control(TaskAction.Verify);
}
//Add EEG channels, if configured
if (Properties.Settings.Default.UseEEG)
{
eegTask = new Task("eegTask");
for (int i = 0; i < Properties.Settings.Default.EEGNumChannels; ++i)
eegTask.AIChannels.CreateVoltageChannel(Properties.Settings.Default.EEGDevice + "/ai" +
(i).ToString(), "", AITerminalConfiguration.Nrse, -10.0, 10.0, AIVoltageUnits.Volts);
setGain(eegTask, (double)Properties.Settings.Default.EEGGain);
eegTask.Control(TaskAction.Verify);
eegSamplingRate = Properties.Settings.Default.EEGSamplingRate;
}
//Add channel to control Cineplex, if configured
if (checkBox_video.Checked)
triggerTask.DOChannels.CreateChannel(Properties.Settings.Default.CineplexDevice + "/Port0/line0:7", "",
ChannelLineGrouping.OneChannelForAllLines);
//Change gain based on comboBox values (1-100)
for (int i = 0; i < spikeTask.Count; ++i)
setGain(spikeTask[i], Properties.Settings.Default.A2Dgain);
//Verify the Tasks
for (int i = 0; i < spikeTask.Count; ++i)
spikeTask[i].Control(TaskAction.Verify);
//if (Properties.Settings.Default.UseSingleChannelPlayback)
// spikeOutTask.Control(TaskAction.Verify);
//Get sampling rates, set to private variables
spikeSamplingRate = Properties.Settings.Default.RawSampleFrequency;
lfpSamplingRate = Properties.Settings.Default.LFPSampleFrequency;
//Version with videoTask as master clock
if (Properties.Settings.Default.UseCineplex)
{
for (int i = 0; i < spikeTask.Count; ++i)
{
spikeTask[i].Timing.ReferenceClockSource = videoTask.Timing.ReferenceClockSource;
spikeTask[i].Timing.ReferenceClockRate = videoTask.Timing.ReferenceClockRate;
}
}
else
{
string masterclock = "/" + Properties.Settings.Default.AnalogInDevice[0].ToString() + "/10MhzRefClock";//"OnboardClock";//
if (!Properties.Settings.Default.UseStimulator)
{
//Deal with non M-series devices (these can't use "ReferenceClockSource"
Device analogInDevice = DaqSystem.Local.LoadDevice(Properties.Settings.Default.AnalogInDevice[0]);
if (analogInDevice.ProductCategory == ProductCategory.MSeriesDaq || analogInDevice.ProductCategory == ProductCategory.XSeriesDaq)
spikeTask[0].Timing.ReferenceClockSource = masterclock; //This will be the master clock
}
else
{
spikeTask[0].Timing.ReferenceClockSource = masterclock;//stimPulseTask.Timing.ReferenceClockSource;
spikeTask[0].Timing.ReferenceClockRate = 10000000.0; //stimPulseTask.Timing.ReferenceClockRate;
}
for (int i = 1; i < spikeTask.Count; ++i) //Set other analog in tasks to master clock
{
spikeTask[i].Timing.ReferenceClockSource = spikeTask[0].Timing.ReferenceClockSource;
spikeTask[i].Timing.ReferenceClockRate = spikeTask[0].Timing.ReferenceClockRate;
}
}
spikeTask[0].Timing.ConfigureSampleClock("", spikeSamplingRate,
SampleClockActiveEdge.Rising, SampleQuantityMode.ContinuousSamples, Convert.ToInt32(Properties.Settings.Default.RawSampleFrequency / 2));
for (int i = 1; i < spikeTask.Count; ++i)
{
//Pipe ai dev0's sample clock to slave devices
spikeTask[i].Timing.ConfigureSampleClock("/" + Properties.Settings.Default.AnalogInDevice[0] + "/ai/SampleClock", spikeSamplingRate,
SampleClockActiveEdge.Rising, SampleQuantityMode.ContinuousSamples, Convert.ToInt32(Properties.Settings.Default.RawSampleFrequency / 2));
//Trigger off of ai dev0's trigger
spikeTask[i].Triggers.StartTrigger.ConfigureDigitalEdgeTrigger("/" + Properties.Settings.Default.AnalogInDevice[0] +
"/ai/StartTrigger", DigitalEdgeStartTriggerEdge.Rising);
// Manually allocate buffer memory
//spikeTask[i].Stream.Buffer.InputBufferSize = DAQ_BUFFER_SIZE_SAMPLES;
}
if (Properties.Settings.Default.SeparateLFPBoard && Properties.Settings.Default.UseLFPs)
{
lfpTask.Timing.ReferenceClockSource = spikeTask[0].Timing.ReferenceClockSource;
lfpTask.Timing.ReferenceClockRate = spikeTask[0].Timing.ReferenceClockRate;
lfpTask.Timing.ConfigureSampleClock("", lfpSamplingRate,
SampleClockActiveEdge.Rising, SampleQuantityMode.ContinuousSamples, Convert.ToInt32(Properties.Settings.Default.LFPSampleFrequency / 2));
// Manually allocate buffer memory
//lfpTask.Stream.Buffer.InputBufferSize = DAQ_BUFFER_SIZE_SAMPLES;
}
else
{
Properties.Settings.Default.numLFPTasks = Properties.Settings.Default.numSpikeTasks;
}
if (Properties.Settings.Default.UseEEG)
{
eegTask.Timing.ReferenceClockSource = spikeTask[0].Timing.ReferenceClockSource;
eegTask.Timing.ReferenceClockRate = spikeTask[0].Timing.ReferenceClockRate;
eegTask.Timing.ConfigureSampleClock("", eegSamplingRate,
SampleClockActiveEdge.Rising, SampleQuantityMode.ContinuousSamples, Convert.ToInt32(Convert.ToDouble(Properties.Settings.Default.EEGSamplingRate) / 2));
// Manually allocate buffer memory
//eegTask.Stream.Buffer.InputBufferSize = DAQ_BUFFER_SIZE_SAMPLES;
}
if (Properties.Settings.Default.UseCineplex)
{
if (checkBox_video.Checked)
{
triggerTask.Timing.ConfigureSampleClock("/" + Properties.Settings.Default.AnalogInDevice[0] + "/ai/SampleClock",
spikeSamplingRate, SampleClockActiveEdge.Rising, SampleQuantityMode.FiniteSamples,
3);
}
if (Properties.Settings.Default.SeparateLFPBoard && Properties.Settings.Default.UseLFPs)
{
lfpTask.Triggers.StartTrigger.ConfigureDigitalEdgeTrigger("/" +
Properties.Settings.Default.AnalogInDevice[0] + "/ai/StartTrigger",
DigitalEdgeStartTriggerEdge.Rising);
}
if (Properties.Settings.Default.UseEEG)
{
eegTask.Triggers.StartTrigger.ConfigureDigitalEdgeTrigger("/" +
Properties.Settings.Default.AnalogInDevice[0] + "/ai/StartTrigger",
DigitalEdgeStartTriggerEdge.Rising);
}
}
if (Properties.Settings.Default.UseStimulator && Properties.Settings.Default.RecordStimTimes)
{
try
{
numStimReads = new List<int>(numDevices);
for (int i = 0; i < spikeTask.Count; ++i)
numStimReads.Add(0);
stimTimeTask = new Task("stimTimeTask");
stimTimeTask.AIChannels.CreateVoltageChannel(Properties.Settings.Default.StimInfoDevice + "/ai16", "",
AITerminalConfiguration.Nrse, -10.0, 10.0, AIVoltageUnits.Volts);
stimTimeTask.AIChannels.CreateVoltageChannel(Properties.Settings.Default.StimInfoDevice + "/ai0", "", AITerminalConfiguration.Nrse,
-10.0, 10.0, AIVoltageUnits.Volts); //For triggers
// Pipe the spikeTasks sample clock to PFI14 on the stim board
DaqSystem.Local.ConnectTerminals(spikeTask[0].Timing.ReferenceClockSource,
"/" + Properties.Settings.Default.StimulatorDevice.ToString() + "/PFI0");
if (isNormalRecording)
stimTimeTask.Timing.ReferenceClockSource = "/" + Properties.Settings.Default.StimulatorDevice.ToString() + "/PFI0";
else
stimTimeTask.Timing.ReferenceClockSource = spikeTask[0].Timing.ReferenceClockSource;
stimTimeTask.Timing.ReferenceClockRate = spikeTask[0].Timing.ReferenceClockRate;
stimTimeTask.Timing.ConfigureSampleClock("", spikeSamplingRate,
SampleClockActiveEdge.Rising, SampleQuantityMode.ContinuousSamples, Convert.ToInt32(Properties.Settings.Default.RawSampleFrequency / 2));
stimTimeTask.Triggers.StartTrigger.ConfigureDigitalEdgeTrigger(
"/" + Properties.Settings.Default.AnalogInDevice[0] + "/ai/StartTrigger", DigitalEdgeStartTriggerEdge.Rising);
stimTimeTask.Control(TaskAction.Verify);
// stim Timing Channel settings object
StringCollection stimTimePhysChan = new StringCollection();
for (int i = 0; i < stimTimeTask.AIChannels.Count; ++i)
{
stimTimePhysChan.Add(stimTimeTask.AIChannels[i].PhysicalName);
}
// Write down the indicies corresponding to the portion of this task that will
// actually record stimulus infromation instead of aux analog input
stimTimeChanSet = new NRAIChannelCollection(stimTimePhysChan);
int[] stimTimeChannels = new int[] { 0, 1 };
stimTimeChanSet.SetupNumericalChannelOnly(stimTimeChannels);
// Manually allocate buffer memory
//stimTimeTask.Stream.Buffer.InputBufferSize = DAQ_BUFFER_SIZE_SAMPLES;
Console.WriteLine("NRAcquisitionSetup complete");
}
catch (Exception e)
{
MessageBox.Show(e.Message);
}
}
//Setup scaling coefficients (to convert digital values to voltages)
scalingCoeffsSpikes = new List<double[]>(spikeTask.Count);
for (int i = 0; i < spikeTask.Count; ++i)
scalingCoeffsSpikes.Add(spikeTask[0].AIChannels[0].DeviceScalingCoefficients);
if (Properties.Settings.Default.SeparateLFPBoard)
scalingCoeffsLFPs = lfpTask.AIChannels[0].DeviceScalingCoefficients;
if (Properties.Settings.Default.UseEEG)
scalingCoeffsEEG = eegTask.AIChannels[0].DeviceScalingCoefficients;
// Setup auxiliary recording tasks
if (Properties.Settings.Default.useAuxAnalogInput)
{
// Set up the aux channel set
auxChanSet = new NRAIChannelCollection(Properties.Settings.Default.auxAnalogInChan);
if (Properties.Settings.Default.auxAnalogInDev == Properties.Settings.Default.StimInfoDevice
&& Properties.Settings.Default.RecordStimTimes)
{
// In this case we are recording both stimulus times and aux analog input times on the same
// DAQ, so we need to just make the auxAnInTask reference the stimulus timing task
twoAITasksOnSingleBoard = true;
auxAnInTask = stimTimeTask;
auxChanSet.SetupAuxCollection(ref auxAnInTask);
}
else
{
// In this case there is no conflict for AI, so we can create a dedicated task for aux analog input
twoAITasksOnSingleBoard = false;
auxAnInTask = new Task("AuxiliaryAnalogInput");
auxChanSet.SetupAuxCollection(ref auxAnInTask);
auxAnInTask.Timing.ReferenceClockSource = spikeTask[0].Timing.ReferenceClockSource;
auxAnInTask.Timing.ReferenceClockRate = spikeTask[0].Timing.ReferenceClockRate;
//Pipe ai dev0's sample clock to slave devices
auxAnInTask.Timing.ConfigureSampleClock("", spikeSamplingRate,
SampleClockActiveEdge.Rising, SampleQuantityMode.ContinuousSamples, Convert.ToInt32(Properties.Settings.Default.RawSampleFrequency / 2));
auxAnInTask.Triggers.StartTrigger.ConfigureDigitalEdgeTrigger("/Dev1/ai/StartTrigger", DigitalEdgeStartTriggerEdge.Rising);
// Manually allocate buffer memory
// auxAnInTask.Stream.Buffer.InputBufferSize = DAQ_BUFFER_SIZE_SAMPLES;
// Create space for the buffer
auxAnData = new double[auxChanSet.numericalChannels.Length, spikeBufferLength];
}
}
if (Properties.Settings.Default.useAuxDigitalInput)
{
auxDigInTask = new Task("AuxiliaryDigitalInput");
auxDigInTask.DIChannels.CreateChannel(Properties.Settings.Default.auxDigitalInPort,
"Auxiliary Digitial In", ChannelLineGrouping.OneChannelForAllLines);
auxDigInTask.Timing.ConfigureSampleClock("", spikeSamplingRate,
SampleClockActiveEdge.Rising, SampleQuantityMode.ContinuousSamples, Convert.ToInt32(Properties.Settings.Default.RawSampleFrequency / 2));
auxDigInTask.Timing.SampleClockSource = spikeTask[0].Timing.SampleClockTerminal;
// Manually allocate buffer memory
// auxDigInTask.Stream.Buffer.InputBufferSize = DAQ_BUFFER_SIZE_SAMPLES;
}
#region Setup_Plotting
numSnipsDisplayed = (int)numericUpDown_NumSnipsDisplayed.Value;
#region PlotData_Buffers
//***********************
//Make PlotData buffers
//***********************
int downsample, numRows, numCols;
const double spikeplotlength = 0.25; //in seconds
switch (Properties.Settings.Default.NumChannels)
{
case 16:
numRows = numCols = 4;
downsample = 10;
break;
case 32:
numRows = numCols = 6;
downsample = 15;
break;
case 64:
numRows = numCols = 8;
downsample = 20; //if this gets really small, LFP data won't plot
break;
default:
numRows = numCols = 4;
downsample = 5;
break;
}
//Create plot colormap
NRBrainbow = (64).GenerateBrainbow();
NRSnipBrainbow = (64).GenerateSnipBrainbow();
NRUnitBrainbow = (64).GenerateUnitBrainbow();
//Initialize graphs
if (spikeGraph != null) { spikeGraph.Dispose(); spikeGraph = null; }
spikeGraph = new GridGraph();
int samplesPerPlot = (int)(Math.Ceiling(Properties.Settings.Default.ADCPollingPeriodSec * spikeSamplingRate / downsample) * (spikeplotlength / Properties.Settings.Default.ADCPollingPeriodSec));
spikeGraph.setup(numRows, numCols, samplesPerPlot, false, 1 / 4.0, spikeTask[0].AIChannels.All.RangeHigh * 2.0);
spikeGraph.setMinMax(0, (float)(samplesPerPlot * numCols) - 1,
(float)(spikeTask[0].AIChannels.All.RangeLow * (numRows * 2 - 1)), (float)(spikeTask[0].AIChannels.All.RangeHigh));
spikeGraph.Dock = DockStyle.Fill;
spikeGraph.Parent = tabPage_spikes;
if (Properties.Settings.Default.UseLFPs)
{
if (lfpGraph != null) { lfpGraph.Dispose(); lfpGraph = null; }
lfpGraph = new RowGraph();
lfpGraph.setup(numChannels, (int)((Math.Ceiling(Properties.Settings.Default.ADCPollingPeriodSec * lfpSamplingRate / downsample) * (5 / Properties.Settings.Default.ADCPollingPeriodSec))),
5.0, spikeTask[0].AIChannels.All.RangeHigh * 2.0);
if (Properties.Settings.Default.SeparateLFPBoard)
lfpGraph.setMinMax(0, 5 * (int)(Math.Ceiling(Properties.Settings.Default.ADCPollingPeriodSec * lfpSamplingRate / downsample) / Properties.Settings.Default.ADCPollingPeriodSec) - 1,
(float)(lfpTask.AIChannels.All.RangeLow * (numChannels * 2 - 1)), (float)(lfpTask.AIChannels.All.RangeHigh));
else
lfpGraph.setMinMax(0, 5 * (int)(Math.Ceiling(Properties.Settings.Default.ADCPollingPeriodSec * lfpSamplingRate / downsample) / Properties.Settings.Default.ADCPollingPeriodSec) - 1,
(float)(spikeTask[0].AIChannels.All.RangeLow * (numChannels * 2 - 1)), (float)(spikeTask[0].AIChannels.All.RangeHigh));
lfpGraph.Dock = DockStyle.Fill;
lfpGraph.Parent = tabPage_LFPs;
}
if (Properties.Settings.Default.ProcessMUA)
{
if (muaGraph != null) { muaGraph.Dispose(); muaGraph = null; }
muaGraph = new RowGraph();
muaGraph.setup(numChannels, (int)((Math.Ceiling(Properties.Settings.Default.ADCPollingPeriodSec * muaSamplingRate / downsample) * (5 / Properties.Settings.Default.ADCPollingPeriodSec))),
5.0, spikeTask[0].AIChannels.All.RangeHigh * 2.0);
muaGraph.setMinMax(0, 5 * (int)(Math.Ceiling(Properties.Settings.Default.ADCPollingPeriodSec * muaSamplingRate / downsample) / Properties.Settings.Default.ADCPollingPeriodSec) - 1,
(float)(spikeTask[0].AIChannels.All.RangeLow * (numChannels * 2 - 1)), (float)(spikeTask[0].AIChannels.All.RangeHigh));
muaGraph.Dock = DockStyle.Fill;
muaGraph.Parent = tabPage_MUA;
muaPlotData = new PlotDataRows(numChannels, downsample, (int)(muaSamplingRate * 5), muaSamplingRate,
(float)spikeTask[0].AIChannels.All.RangeHigh * 2F, 0.5, 5, Properties.Settings.Default.ADCPollingPeriodSec);
//muaPlotData.setGain(Properties.Settings.Default.LFPDisplayGain);
//muaGraph.setDisplayGain(Properties.Settings.Default.LFPDisplayGain);
muaPlotData.dataAcquired += new PlotData.dataAcquiredHandler(muaPlotData_dataAcquired);
}
if (Properties.Settings.Default.UseEEG)
{
if (eegGraph != null) { eegGraph.Dispose(); eegGraph = null; }
eegGraph = new RowGraph();
eegGraph.setup(Properties.Settings.Default.EEGNumChannels, (int)((Math.Ceiling(Properties.Settings.Default.ADCPollingPeriodSec * eegSamplingRate / downsample) * (5 / Properties.Settings.Default.ADCPollingPeriodSec))),
5.0, eegTask.AIChannels.All.RangeHigh * 2.0);
eegGraph.setMinMax(0, 5 * (int)(Math.Ceiling(Properties.Settings.Default.ADCPollingPeriodSec * eegSamplingRate / downsample) / Properties.Settings.Default.ADCPollingPeriodSec) - 1,
(float)(eegTask.AIChannels.All.RangeLow * (Properties.Settings.Default.EEGNumChannels * 2 - 1)), (float)(eegTask.AIChannels.All.RangeHigh));
eegGraph.Dock = DockStyle.Fill;
eegGraph.Parent = tabPage_EEG;
}
resetSpkWfm(); //Take care of spike waveform graph
double ampdec = (1 / Properties.Settings.Default.PreAmpGain);
spikePlotData = new PlotDataGrid(numChannels, downsample, (int)(spikeSamplingRate), spikeSamplingRate,
(float)(spikeTask[0].AIChannels.All.RangeHigh * 2.0), numRows, numCols, spikeplotlength,
Properties.Settings.Default.ChannelMapping, Properties.Settings.Default.ADCPollingPeriodSec);
spikePlotData.dataAcquired += new PlotData.dataAcquiredHandler(spikePlotData_dataAcquired);
spikePlotData.setGain(Properties.Settings.Default.SpikeDisplayGain);
spikeGraph.setDisplayGain(Properties.Settings.Default.SpikeDisplayGain);
if (Properties.Settings.Default.UseLFPs)
{
if (Properties.Settings.Default.SeparateLFPBoard)
lfpPlotData = new PlotDataRows(numChannels, downsample, (int)(lfpSamplingRate * 5), lfpSamplingRate,
(float)lfpTask.AIChannels.All.RangeHigh * 2F, 0.5, 5, Properties.Settings.Default.ADCPollingPeriodSec);
else lfpPlotData = new PlotDataRows(numChannels, downsample, (int)(lfpSamplingRate * 5), lfpSamplingRate,
(float)spikeTask[0].AIChannels.All.RangeHigh * 2F, 0.5, 5, Properties.Settings.Default.ADCPollingPeriodSec);
lfpPlotData.setGain(Properties.Settings.Default.LFPDisplayGain);
lfpGraph.setDisplayGain(Properties.Settings.Default.LFPDisplayGain);
lfpPlotData.dataAcquired += new PlotData.dataAcquiredHandler(lfpPlotData_dataAcquired);
}
waveformPlotData = new EventPlotData(numChannels, spikeDet.NumPre + spikeDet.NumPost + 1, (float)(spikeTask[0].AIChannels.All.RangeHigh * 2F),
numRows, numCols, numSnipsDisplayed, Properties.Settings.Default.ChannelMapping);
waveformPlotData.setGain(Properties.Settings.Default.SpkWfmDisplayGain);
spkWfmGraph.setDisplayGain(Properties.Settings.Default.SpkWfmDisplayGain);
waveformPlotData.dataAcquired += new EventPlotData.dataAcquiredHandler(waveformPlotData_dataAcquired);
waveformPlotData.start();
#endregion
if (Properties.Settings.Default.UseEEG)
{
eegPlotData = new PlotDataRows(Properties.Settings.Default.EEGNumChannels, downsample, (int)(eegSamplingRate * 5), eegSamplingRate,
(float)eegTask.AIChannels.All.RangeHigh * 2F, 0.5, 5, Properties.Settings.Default.ADCPollingPeriodSec);
eegPlotData.setGain(Properties.Settings.Default.EEGDisplayGain);
eegGraph.setDisplayGain(Properties.Settings.Default.EEGDisplayGain);
eegPlotData.dataAcquired += new PlotData.dataAcquiredHandler(eegPlotData_dataAcquired);
}
if (Properties.Settings.Default.useAuxAnalogInput)
{
// Remove existing plots
for (int i = scatterGraph_AuxAnalogData.Plots.Count-1; i > 0; --i)
{
scatterGraph_AuxAnalogData.Plots.RemoveAt(i);
}
// Initialize the aux data scatter graph with a plot for each aux Analog channel
for (int i = 0; i < Properties.Settings.Default.auxAnalogInChan.Count-1; ++i)
{
ScatterPlot p = new ScatterPlot();
scatterGraph_AuxAnalogData.Plots.Add(p);
}
// Initialize the controller
auxInputGraphController = new ScatterGraphController(ref scatterGraph_AuxAnalogData);
// Make history selector reflect current limits on input
//slide_AnalogDispMaxVoltage.Range = new Range(0.05, 10);
//slide_AnalogDispWidth.Range = new Range(2*Properties.Settings.Default.ADCPollingPeriodSec, Properties.Settings.Default.datSrvBufferSizeSec);
}
#endregion
#region Setup_Filters
//Setup filters, based on user's input
resetSpikeFilter();
if (Properties.Settings.Default.UseLFPs) resetLFPFilter();
resetEEGFilter();
muaFilter = new Filters.MUAFilter(
numChannels, spikeSamplingRate, spikeBufferLength,
Properties.Settings.Default.MUAHighCutHz,
Properties.Settings.Default.MUAFilterOrder,
MUA_DOWNSAMPLE_FACTOR,
Properties.Settings.Default.ADCPollingPeriodSec);
#endregion
#region Setup_DataStorage
//Initialize data storing matrices
// numChannels = Properties.Settings.Default.NumChannels;
numSpikeReads = new int[spikeTask.Count];
filtSpikeData = new rawType[numChannels][];
if (Properties.Settings.Default.UseLFPs)
{
filtLFPData = new rawType[numChannels][];
finalLFPData = new rawType[numChannels][];
for (int i = 0; i < filtSpikeData.GetLength(0); ++i)
{
if (Properties.Settings.Default.SeparateLFPBoard)
filtLFPData[i] = new rawType[lfpBufferLength];
else
filtLFPData[i] = new rawType[spikeBufferLength];
}
}
if (Properties.Settings.Default.ProcessMUA)
{
muaData = new double[numChannels][];
for (int c = 0; c < numChannels; ++c)
muaData[c] = new double[spikeBufferLength / MUA_DOWNSAMPLE_FACTOR];
}
if (Properties.Settings.Default.UseEEG)
{
filtEEGData = new double[Properties.Settings.Default.EEGNumChannels][];
for (int i = 0; i < filtEEGData.GetLength(0); ++i)
{
filtEEGData[i] = new double[eegBufferLength];
}
}
for (int i = 0; i < filtSpikeData.GetLength(0); ++i)
{
filtSpikeData[i] = new rawType[spikeBufferLength];
if (Properties.Settings.Default.UseLFPs)
finalLFPData[i] = new rawType[lfpBufferLength];
}
if (Properties.Settings.Default.UseStimulator)
{
stimDataBuffer = new double[STIM_BUFFER_LENGTH];
stimJump = (double)spikeSamplingRate * 0.0001; //num. indices in 100 us of data
}
stimIndices = new List<StimTick>(5);
//if devices refresh rate is reset, need to reset SALPA
if (checkBox_SALPA.Checked)
resetSALPA();
if (spikeDet != null && isNormalRecording)
setSpikeDetector();
if (spikeDet.spikeDetector == null)
setSpikeDetector();
#endregion
#region Verify Tasks
if (Properties.Settings.Default.UseStimulator && Properties.Settings.Default.RecordStimTimes)
stimTimeTask.Control(TaskAction.Verify);
if (Properties.Settings.Default.UseEEG)
eegTask.Control(TaskAction.Verify);
if (Properties.Settings.Default.SeparateLFPBoard && Properties.Settings.Default.UseLFPs)
lfpTask.Control(TaskAction.Verify);
if (checkBox_video.Checked)
triggerTask.Control(TaskAction.Verify);
for (int i = 0; i < spikeTask.Count; ++i)
spikeTask[i].Control(TaskAction.Verify);
if (Properties.Settings.Default.useAuxAnalogInput)
auxAnInTask.Control(TaskAction.Verify);
if (Properties.Settings.Default.useAuxDigitalInput)
auxDigInTask.Control(TaskAction.Verify);
#endregion
SetupFileWriting();
//Set callbacks for data acq.
spikeReader = new List<AnalogMultiChannelReader>(spikeTask.Count);
for (int i = 0; i < spikeTask.Count; ++i)
{
spikeReader.Add(new AnalogMultiChannelReader(spikeTask[i].Stream));
spikeReader[i].SynchronizeCallbacks = true;
}
//if (Properties.Settings.Default.UseSingleChannelPlayback)
// spikeOutWriter = new AnalogSingleChannelWriter(spikeOutTask.Stream);
if (checkBox_video.Checked)
triggerWriter = new DigitalSingleChannelWriter(triggerTask.Stream);
//if (Properties.Settings.Default.UseSingleChannelPlayback)
// spikeOutWriter.SynchronizeCallbacks = false; //These don't use UI, so they don't need to be synched
spikeCallback = new AsyncCallback(AnalogInCallback_spikes);
if (Properties.Settings.Default.UseStimulator && Properties.Settings.Default.RecordStimTimes)
{
stimTimeReader = new AnalogMultiChannelReader(stimTimeTask.Stream);
}
if (Properties.Settings.Default.SeparateLFPBoard && Properties.Settings.Default.UseLFPs)
{
lfpReader = new AnalogUnscaledReader(lfpTask.Stream);
lfpReader.SynchronizeCallbacks = true;
lfpCallback = new AsyncCallback(AnalogInCallback_LFPs);
}
if (Properties.Settings.Default.UseEEG)
{
eegReader = new AnalogUnscaledReader(eegTask.Stream);
eegReader.SynchronizeCallbacks = true;
eegCallback = new AsyncCallback(AnalogInCallback_EEG);
}
if (Properties.Settings.Default.useAuxAnalogInput)
{
auxAnReader = new AnalogMultiChannelReader(auxAnInTask.Stream);
auxAnReader.SynchronizeCallbacks = true;
auxAnCallback = new AsyncCallback(AnalogInCallback_AuxAn);
}
if (Properties.Settings.Default.useAuxDigitalInput)
{
auxDigReader = new DigitalSingleChannelReader(auxDigInTask.Stream);
auxDigReader.SynchronizeCallbacks = true;
auxDigCallback = new AsyncCallback(AnalogInCallback_AuxDig);
}
//Setup background workers for data processing
bwSpikes = new List<BackgroundWorker>(spikeTask.Count);
bwIsRunning = new bool[spikeTask.Count];
for (int i = 0; i < spikeTask.Count; ++i)
{
bwSpikes.Add(new BackgroundWorker());
bwSpikes[i].DoWork += new DoWorkEventHandler(bwSpikes_DoWork);
bwSpikes[i].RunWorkerCompleted += new RunWorkerCompletedEventHandler(bwSpikes_RunWorkerCompleted);
bwSpikes[i].WorkerSupportsCancellation = true;
}
//Make persistent buffers for spikeData
spikeData = new List<AnalogWaveform<double>[]>(spikeReader.Count);
for (int i = 0; i < spikeReader.Count; ++i)
{
spikeData.Add(new AnalogWaveform<double>[numChannelsPerDev]);
for (int j = 0; j < numChannelsPerDev; ++j)
spikeData[i][j] = new AnalogWaveform<double>(spikeBufferLength);
}
//Make channel playback task
if (Properties.Settings.Default.UseSingleChannelPlayback)
BNCOutput = new ChannelOutput(spikeSamplingRate, 0.1, Properties.Settings.Default.ADCPollingPeriodSec, spikeTask[0],
Properties.Settings.Default.SingleChannelPlaybackDevice, 0);
}
catch (Exception exception)
{
//Display Errors
this.Cursor = Cursors.Default;
MessageBox.Show(exception.Message);
reset();
}
// Set up the DataSrv object. This is an object that publishes a nice large data history
// for use in closed loop control and other things
if (datSrv != null)
datSrv = null;
datSrv = new DataSrv(
Properties.Settings.Default.datSrvBufferSizeSec,
checkBox_SALPA.Checked,
SALPA_WIDTH,
checkBox_spikesFilter.Checked,
spikeDet.spikeDetectionLag
);
// Set the number of units if appropriate
if (spikeDet.spikeSorter != null)
datSrv.SetNumberOfUnits(spikeDet.spikeSorter.totalNumberOfUnits, spikeDet.spikeSorter.unit2Channel);
Debugger = new Logger();
Debugger.GrabTimer(spikeTask[0]);
//Send debug output to the user's application data folder
Debugger.SetPath(Path.Combine(Properties.Settings.Default.neurorighterAppDataPath, "neurorighter-log.txt"));
//Tell neuroRighter that the tasks now exist
taskRunning = true;
}
else
{
Console.WriteLine("NRAcquisitionSetup was called while a task was running, and therefore setup did not execute. Perhaps this should have thrown an error");
}
}
//update gui at the end
// Modify the UI, so user doesn't try running multiple instances of tasks
spikeDet.numPreSamples.Enabled = false;
spikeDet.numPostSamples.Enabled = false;
settingsToolStripMenuItem.Enabled = false;
button_Train.Enabled = false;
button_SetRecordingStreams.Enabled = false;
switch_record.Enabled = false;
//processingSettingsToolStripMenuItem.Enabled = false;
button_startStimFromFile.Enabled = false;
button_startClosedLoopStim.Enabled = false;
checkBox_SALPA.Enabled = false;
//numericUpDown_NumSnipsDisplayed.Enabled = false;
button_startClosedLoopStim.Enabled = false;
button_scaleUp.Enabled = true;
button_scaleDown.Enabled = true;
button_scaleReset.Enabled = true;
// Disable spike detector saving while running
spikeDet.DisableFileMenu();
Console.WriteLine("NRAcquisitionSetup successfully executed");
this.Cursor = Cursors.Default;
return false;
}