/// <summary>
/// Flushes memory of fqprotobin files
/// </summary>
/// <param name="sender"></param>
/// <param name="e"></param>
private void flushMemoryToolStripMenuItem_Click(object sender, EventArgs e)
{
if (FastqController.getInstance().FlushMemoryOfProtobinFiles() == true)
{
UserResponse.InformationResponse("Protobin directory cleaned.");
}
}
/// <summary>
/// Resets the state of a controller for loading a new file
/// </summary>
public void PrimeForNewFile()
{
fqFileMap = null;
if (FlushMemoryOfProtobinFiles() == true)
{
UserResponse.InformationResponse("Protobin directory cleaned.");
}
}
/// <summary>
/// Method allows the cancellation of backgroundworker thread for loading and processing files.
/// </summary>
/// <param name="sender"></param>
/// <param name="e"></param>
public void loadWorker_Completed(object sender, RunWorkerCompletedEventArgs e)
{
if (e.Error != null)
{
UserResponse.ErrorResponse(e.Error.Message, "Load Error");
}
if (e.Cancelled)
{
UserResponse.InformationResponse("Loading cancelled at request");
}
FastqController.CONTROLLER_STATE = FastqController.FastqControllerState.STATE_READY;
Console.WriteLine("Process ended.");
}
/// <summary>
/// Parses file into component chunks through the parseFastq class before handing component details to FastqController
/// for processing.
/// </summary>
/// <param name="sender"></param>
/// <param name="e"></param>
private void loadWorker_DoWork(object sender, DoWorkEventArgs e)
{
BackgroundWorker worker = sender as BackgroundWorker;
InputFq input = (InputFq)e.Argument;
FileStream fileStream = input.fileStream;
String fileName = input.fileName;
ParseFastq parseFq;
if ((worker.CancellationPending == true))
{
e.Cancel = true;
}
else
{
Stopwatch stopwatch = new Stopwatch();
stopwatch.Start();
try
{
worker.ReportProgress(3, "[PARSING FILE]");
parseFq = new ParseFastq(fileStream, fileName);
if (parseFq.getFastqFileCheck() == true && FastqController.CONTROLLER_STATE == FastqController.FastqControllerState.STATE_READY)
{
//Create new fqFileMap in controller and prime state for load
FastqController.CONTROLLER_STATE = FastqController.FastqControllerState.PARSING;
FastqController.getInstance().CreateNewFastqFile(fileName, parseFq.GetFastqFileLength());
FastqController.getInstance().GetFqFileMap().InitializeReadMap();
int fqFileComponentNumber = 1;
ProtocolBuffersSerialization protoBuf = new ProtocolBuffersSerialization();
GenericFastqInputs processInputs = new GenericFastqInputs();
processInputs.TaskAction = Task_LoadTask.statement;
ITaskStrategy task = TaskDiscrimination.getTask(processInputs.TaskAction);
// Uses IEnummerable yield return to parse components back to this class via this foreach loop
foreach (FqFile_Component fqFileComponent in parseFq.ParseComponents())
{
Double progressPercent = (Double)(((fqFileComponentNumber - 1) * FqFileMap.FQ_BLOCK_LIMIT) / (Double)(parseFq.GetLineCount() / 4));
worker.ReportProgress((int)(progressPercent * 100), ParseFastq.REPORT_STATEMENT);
if (fqFileComponent.getFastqArraySize() >= 1)
{
int threadId;
String componentFileName = FastqController.getInstance().GetFqFileMap().FileGUID + "_" + Path.GetFileNameWithoutExtension(fileName) + "_" + fqFileComponentNumber + ProtocolBuffersSerialization.PROTOBUF_FILE_EXTENSION;
fqFileComponent.setFastqFileName(componentFileName);
fqFileComponent.setComponentNumber(fqFileComponentNumber);
fqFileComponent.setFqHashMap(FastqController.getInstance().GetFqFileMap().FqReadMap);
processInputs.FastqFile = fqFileComponent;
processInputs = task.perform(processInputs);
FastqController.getInstance().BuildFqFileMap(processInputs.FastqFile);
ProtocolBuffersSerialization.ProbufSerializeFqFile_AsyncMethodCaller caller
= new ProtocolBuffersSerialization.ProbufSerializeFqFile_AsyncMethodCaller(protoBuf.ProtobufSerializeFqFile);
IAsyncResult result = caller.BeginInvoke((processInputs.FastqFile as FqFile_Component), componentFileName, out threadId, null, null);
Boolean returnValue = caller.EndInvoke(out threadId, result);
if (returnValue == false)
{
UserResponse.ErrorResponse("File serialization methods failed, please check you have hard disk space and restart the application", "File Error");
Console.WriteLine("Serialization failed");
loadWorker.CancelAsync();
}
else
{
FastqController.getInstance().addFqFileComponentDirectory(componentFileName);
}
fqFileComponentNumber++;
}
}
parseFq.CloseReader();
task.confirmTaskEnd();
Console.WriteLine("\n*********\n");
FastqController.getInstance().GetFqFileMap().CalculateGlobalFileScores();
FastqController.getInstance().GetFqFileMap().GlobalDetails.OutputToConsole();
loadWorker.ReportProgress(100, task.getReportStatement());
stopwatch.Stop();
Console.WriteLine("Task: {0} Completed in Time: {1}", task.getStatement(), stopwatch.Elapsed);
FastqController.getInstance().GetFqFileMap().LastTask = processInputs.TaskAction;
FastqController.getInstance().GetFqFileMap().TimeTaken = stopwatch.Elapsed.ToString();
this.UpdateGUIThread(processInputs);
}
else
{
worker.ReportProgress(0, "");
UserResponse.InformationResponse("File does not conform to standard format.", "File Error");
parseFq.CloseReader();
}
}
catch (IOException exception)
{
Console.Write(exception.StackTrace);
UserResponse.ErrorResponse(exception.ToString());
}
catch (InsufficientMemoryException exception)
{
Console.Write(exception.StackTrace);
UserResponse.ErrorResponse(exception.ToString());
}
catch (OutOfMemoryException exception)
{
Console.Write(exception.StackTrace);
UserResponse.ErrorResponse(exception.ToString());
}
catch (ArithmeticException exception)
{
Console.Write(exception.StackTrace);
UserResponse.ErrorResponse(exception.ToString());
}
FastqController.CONTROLLER_STATE = FastqController.FastqControllerState.STATE_READY;
stopwatch.Stop();
}
}