private void DisplayScan()
{
if (this.reader == null)
{
return;
}
try
{
this.scan.ValidateComponent();
this.minPeakIntensity.ValidateComponent();
double rt = retentionTimes[this.scan.Value - 1];
PeakList <Peak> pkl = reader.GetPeakList(rt, this.minPeakIntensity.Value, topPeak.Value);
try
{
ZedGraphicExtension.ClearData(this.zgcScan, false);
var pplLight = new PointPairList();
pkl.ForEach(p => pplLight.Add(p.Mz, p.Intensity));
ZedGraphicExtension.AddIndividualLine(this.zgcScan, MyConvert.Format("Scan {0}", this.scan.Value), pplLight, Color.Blue, false);
if (reader.GetMsLevel(rt) == 2)
{
this.zgcScan.GraphPane.Title.Text = MyConvert.Format("Precursor MZ = {0:0.0000}", pkl.PrecursorMZ);
}
else
{
this.zgcScan.GraphPane.Title.Text = string.Empty;
}
}
finally
{
ZedGraphicExtension.UpdateGraph(this.zgcScan);
}
StringBuilder sb = new StringBuilder();
pkl.ForEach(m => sb.AppendLine(MyConvert.Format("{0:0.0000}\t{1:0.00}", m.Mz, m.Intensity)));
txtPeaks.Text = sb.ToString();
}
catch (Exception ex)
{
MessageBox.Show(ex.Message);
}
}
public void Update(object sender, UpdateQuantificationItemEventArgs e)
{
var summary = e.Item as SilacQuantificationSummaryItem;
if (summary == null)
{
throw new ArgumentNullException("UpdateQuantificationItemEventArgs.Item cannot be null");
}
ZedGraphicExtension.ClearData(zgcGraph, false);
try
{
var envelope = summary.ObservedEnvelopes.Find(m => m.IsSelected);
if (envelope == null)
{
return;
}
double minMz = envelope.Light[0].Mz - 1.0;
double maxMz = envelope.Heavy[envelope.Heavy.Count - 1].Mz + 1.0;
zgcGraph.GraphPane.XAxis.Scale.Min = minMz;
zgcGraph.GraphPane.XAxis.Scale.Max = maxMz;
var pplLight = new PointPairList();
envelope.Light.ForEach(p => pplLight.Add(p.Mz, p.Intensity));
var pplHeavy = new PointPairList();
envelope.Heavy.ForEach(p => pplHeavy.Add(p.Mz, p.Intensity));
if (summary.SampleIsLight)
{
ZedGraphicExtension.AddIndividualLine(zgcGraph, "Sample", pplLight, SilacQuantificationConstants.SAMPLE_COLOR, false);
ZedGraphicExtension.AddIndividualLine(zgcGraph, "Reference", pplHeavy, SilacQuantificationConstants.REFERENCE_COLOR, false);
}
else
{
ZedGraphicExtension.AddIndividualLine(zgcGraph, "Sample", pplHeavy, SilacQuantificationConstants.SAMPLE_COLOR, false);
ZedGraphicExtension.AddIndividualLine(zgcGraph, "Reference", pplLight, SilacQuantificationConstants.REFERENCE_COLOR, false);
}
// Shift the text items up by 5 user scale units above the bars
const float shift = 5;
var ppl = new PointPairList();
ppl.AddRange(pplLight);
ppl.AddRange(pplHeavy);
for (int i = 0; i < ppl.Count; i++)
{
// format the label string to have 1 decimal place
string lab = ppl[i].X.ToString("0.00");
// create the text item (assumes the x axis is ordinal or text)
// for negative bars, the label appears just above the zero value
var text = new TextObj(lab, ppl[i].X, ppl[i].Y + shift);
// tell Zedgraph to use user scale units for locating the TextItem
text.Location.CoordinateFrame = CoordType.AxisXYScale;
// AlignH the left-center of the text to the specified point
text.Location.AlignH = AlignH.Left;
text.Location.AlignV = AlignV.Center;
text.FontSpec.Border.IsVisible = false;
text.FontSpec.Fill.IsVisible = false;
// rotate the text 90 degrees
text.FontSpec.Angle = 90;
// add the TextItem to the list
zgcGraph.GraphPane.GraphObjList.Add(text);
}
if (File.Exists(summary.RawFilename))
{
if (rawFile == null)
{
rawFile = new RawFileImpl(summary.RawFilename);
}
else if (!rawFile.FileName.Equals(summary.RawFilename))
{
rawFile.Open(summary.RawFilename);
}
PeakList <Peak> pkl = rawFile.GetPeakList(envelope.Light.ScanTimes[0].Scan);
var pplRaw = new PointPairList();
pplRaw.Add(minMz, 0.0);
for (int i = 0; i < pkl.Count; i++)
{
if (pkl[i].Mz < minMz)
{
continue;
}
else if (pkl[i].Mz > maxMz)
{
break;
}
pplRaw.Add(pkl[i].Mz, -pkl[i].Intensity);
}
pplRaw.Add(maxMz, 0.0);
ZedGraphicExtension.AddIndividualLine(zgcGraph, "PeakList From RawFile", pplRaw, SilacQuantificationConstants.IDENTIFIED_COLOR, true);
}
}
finally
{
ZedGraphicExtension.UpdateGraph(zgcGraph);
}
}