private void Chart1_MouseUp(object sender, MouseEventArgs e)
{
TifFileInfo fi = IA.TabPages.TabCollections[IA.TabPages.SelectedIndex].tifFI;
moveMax = false;
moveMin = false;
applyToHistory(fi.cValue,
fi.MinBrightness[fi.cValue],
fi.MaxBrightness[fi.cValue]);
Chart1.Cursor = Cursors.Default;
//Chart1.Capture = false;
//System.Windows.Forms.Cursor.Clip = new Rectangle(0, 0, 0, 0);
calculateHistogramArray(fi, true);
if (applyToAll.Checked == true & autoDetect.Checked == false)
{
int curC = fi.cValue;
for (int c = 0; c < fi.sizeC; c++)
{
fi.cValue = c;
IA.BandC.calculateHistogramArray(fi, true);
}
fi.cValue = curC;
calculateHistogramArray(fi, true);
}
IA.ReloadImages();
}
private void optionForm_Hide()
{
if (functions.Length > 0)
{
Properties.Settings.Default.CTChart_Functions[IA.TabPages.ActiveAccountIndex] = "@\n" +
string.Join("\n", functions);
}
else
{
Properties.Settings.Default.CTChart_Functions[IA.TabPages.ActiveAccountIndex] = "@";
}
Properties.Settings.Default.Save();
OptionForm.Hide();
LoadFunctions();
IA.ReloadImages();
}
public OtsuSegmentation(Panel mainPanel, ImageAnalyser IA)
{
this.IA = IA;
//Core panel
panel.Dock = DockStyle.Top;
panel.BackColor = IA.FileBrowser.BackGround2Color1;
panel.ForeColor = IA.FileBrowser.ShriftColor1;
panel.Visible = false;
panel.Height = 150;
mainPanel.Controls.Add(panel);
panel.BringToFront();
#region Options
GroupBox optionGB = new GroupBox();
optionGB.Text = "Options:";
optionGB.BackColor = IA.FileBrowser.BackGround2Color1;
optionGB.ForeColor = IA.FileBrowser.ShriftColor1;
optionGB.Dock = DockStyle.Top;
optionGB.Height = 85;
panel.Controls.Add(optionGB);
optionGB.BringToFront();
Label Name = new Label();
Name.Text = "Thresholds:";
Name.Width = TextRenderer.MeasureText(Name.Text, Name.Font).Width;
Name.Location = new Point(5, 18);
optionGB.Controls.Add(Name);
Name.BringToFront();
ComboBox cb = thresholdsNumCB;
cb.Text = "0";
cb.Items.AddRange(new string[] { "0", "1", "2", "3", "4" });
cb.Width = 40;
cb.Location = new Point(80, 15);
optionGB.Controls.Add(cb);
cb.BringToFront();
cb.DropDownStyle = ComboBoxStyle.DropDownList;
cb.SelectedIndex = 0;
cb.AutoSize = false;
cb.SelectedIndexChanged += new EventHandler(delegate(object o, EventArgs e)
{
TifFileInfo fi = IA.TabPages.TabCollections[IA.TabPages.SelectedIndex].tifFI;
fi.thresholdsCBoxIndex[fi.cValue] = cb.SelectedIndex;
IA.ReloadImages();
});
CheckBox checkB = sumHistogramsCheckBox;
checkB.Text = "Use SUM histogram";
checkB.Tag = "Use SUM histogram:\nCalculates histograms for all images\nand merge them into one";
checkB.Width = 150;
checkB.Checked = true;
checkB.MouseHover += Control_MouseOver;
checkB.Location = new Point(7, 35);
optionGB.Controls.Add(checkB);
checkB.BringToFront();
checkB.CheckedChanged += new EventHandler(delegate(object o, EventArgs e)
{
TifFileInfo fi = IA.TabPages.TabCollections[IA.TabPages.SelectedIndex].tifFI;
fi.sumHistogramChecked[fi.cValue] = checkB.Checked;
if (((CheckBox)o).Focused == true)
{
IA.ReloadImages();
}
});
{
Button btn = ProcessBtn;
btn.Width = 115;
btn.FlatStyle = FlatStyle.Standard;
btn.BackColor = SystemColors.ButtonFace;
btn.ForeColor = Color.Black;
btn.Text = "Process";
btn.Location = new Point(5, 58);
optionGB.Controls.Add(btn);
btn.BringToFront();
btn.Click += new EventHandler(delegate(object o, EventArgs a)
{
int MLEVEL = thresholdsNumCB.SelectedIndex + 1;
if (IA.Segmentation.SegmentationCBox.SelectedIndex == 2)
{
IA.Segmentation.Kmeans.Start(MLEVEL);
return;
}
if (IA.Segmentation.SegmentationCBox.SelectedIndex != 1)
{
return;
}
TifFileInfo fi = IA.TabPages.TabCollections[IA.TabPages.SelectedIndex].tifFI;
if (fi.available == false)
{
MessageBox.Show("Image is not ready yet! \nTry again later.");
}
else
{
//background worker
var bgw = new BackgroundWorker();
bgw.WorkerReportsProgress = true;
bgw.DoWork += new DoWorkEventHandler(delegate(Object o1, DoWorkEventArgs a1)
{
try
{
//Segmentation event
run(fi, MLEVEL);
}
catch { }
//report progress
((BackgroundWorker)o1).ReportProgress(0);
});
bgw.ProgressChanged += new ProgressChangedEventHandler(delegate(Object o1, ProgressChangedEventArgs a1)
{
fi.available = true;
IA.FileBrowser.StatusLabel.Text = "Ready";
IA.MarkAsNotSaved();
IA.ReloadImages();
});
//Apply status
IA.FileBrowser.StatusLabel.Text = "Segmentation...";
fi.available = false;
//start bgw
bgw.RunWorkerAsync();
}
});
}
#endregion Options
#region Thresholds
GroupBox threshGB = new GroupBox();
threshGB.Text = "Thresholds:";
threshGB.BackColor = IA.FileBrowser.BackGround2Color1;
threshGB.ForeColor = IA.FileBrowser.ShriftColor1;
threshGB.Dock = DockStyle.Fill;
threshGB.Height = 50;
panel.Controls.Add(threshGB);
threshGB.BringToFront();
//Color btns
Panel colorPanel = new Panel();
colorPanel.Dock = DockStyle.Left;
colorPanel.Width = 25;
threshGB.Controls.Add(colorPanel);
Panel UpPanel = new Panel();
UpPanel.Dock = DockStyle.Top;
UpPanel.Height = 15;
threshGB.Controls.Add(UpPanel);
UpPanel.BringToFront();
for (int i = 0; i < colorBtns.Length; i++)
{
Button btn = new Button();
btn.FlatStyle = FlatStyle.Flat;
btn.FlatAppearance.BorderSize = 0;
btn.ForeColor = Color.Black;
btn.Text = "";
btn.Tag = i;
btn.Dock = DockStyle.Top;
btn.Height = 25;
colorPanel.Controls.Add(btn);
colorBtns[i] = btn;
btn.BringToFront();
btn.Visible = false;
btn.MouseDown += new MouseEventHandler(ColorBtn_Click);
btn.MouseHover += new EventHandler(delegate(object o, EventArgs a)
{
if (btn.Text == "")
{
TurnOnToolTip.SetToolTip(btn, "Color " + ((int)btn.Tag).ToString()
+ ":\nLeft click to Disable\nRight click to change color");
}
else
{
TurnOnToolTip.SetToolTip(btn, "Color " + ((int)btn.Tag).ToString()
+ " - Disabled\nLeft click to Enable");
}
});
}
//threshold track bars
for (int i = 0; i < threshTrackBars.Length; i++)
{
CTTrackBar tb = new CTTrackBar();
tb.Initialize();
tb.Panel.Dock = DockStyle.Top;
tb.BackColor(IA.FileBrowser.BackGround2Color1);
tb.ForeColor(IA.FileBrowser.ShriftColor1);
tb.Panel.Visible = false;
tb.Refresh(0, 0, 10);
tb.Name.Text = "T" + (i + 1).ToString();
tb.NamePanel.Width = 30;
threshGB.Controls.Add(tb.Panel);
threshTrackBars[i] = tb;
tb.Panel.BringToFront();
tb.Value.Changed += new ChangedValueEventHandler(delegate(Object o, ChangeValueEventArgs a)
{
TrackBar_ValueChange(a, tb);
});
}
#endregion Thresholds
}
private void TrackBar_ValueChange(ChangeValueEventArgs e, CTTrackBar tb)
{
//variables
TifFileInfo fi = IA.TabPages.TabCollections[IA.TabPages.SelectedIndex].tifFI;
int[] vals = fi.thresholdValues[fi.cValue];
int val = int.Parse(e.Value);
int valRef = val;
//find trackbar index
int index = 0;
for (int i = 0; i < threshTrackBars.Length; i++)
{
if (threshTrackBars[i] == tb)
{
index = i; break;
}
}
//Check the avaliable min
if (index > 0)
{
if (val <= vals[index])
{
val = vals[index] + 1;
}
}
//Check the avaliable max
if (index < fi.thresholds[fi.cValue] - 1)
{
if (val >= vals[index + 2])
{
val = vals[index + 2] - 1;
}
}
//refresh if value is wrong
if (val != valRef)
{
tb.Refresh(val, tb.TrackBar1.Minimum, tb.TrackBar1.Maximum);
}
//apply changes
if (val != vals[index + 1])
{
if (tb.TrackBar1.Focused == true | tb.TextBox1.Focused == true |
tb.ApplyBtn.Focused == true)
{
#region apply to history
fi.delHist = true;
IA.delHist = true;
IA.UnDoBtn.Enabled = true;
IA.DeleteFromHistory();
fi.History.Add("segmentation.SetThreshOld("
+ fi.cValue + "," + (index + 1).ToString() + ","
+ vals[index + 1].ToString() + ")");
fi.History.Add("segmentation.SetThreshOld("
+ fi.cValue + "," + (index + 1).ToString() + ","
+ val.ToString() + ")");
IA.UpdateUndoBtns();
IA.MarkAsNotSaved();
#endregion apply to history
}
vals[index + 1] = val;
IA.ReloadImages();
}
}
private void Control_MouseDown(object sender, MouseEventArgs e)
{
Button ctr = (Button)sender;
int i = 0;
foreach (Button btn in ColorBtnList)
{
if (btn == ctr)
{
break;
}
i++;
}
if (e.Button == MouseButtons.Left)
{
if (ctr.ImageIndex == 0)
{
int count = 0;
foreach (Button btn in ColorBtnList)
{
if (btn.ImageIndex == 0)
{
count++;
}
}
if (count < 2)
{
return;
}
ctr.ImageIndex = 1;
#region apply to history
TifFileInfo fi = IA.TabPages.TabCollections[IA.TabPages.SelectedIndex].tifFI;
fi.delHist = true;
IA.delHist = true;
IA.UnDoBtn.Enabled = true;
IA.DeleteFromHistory();
fi.History.Add("enableColorChanel("
+ ColorBtnList.IndexOf(ctr).ToString() + ",true)");
fi.History.Add("enableColorChanel("
+ ColorBtnList.IndexOf(ctr).ToString() + ",false)");
IA.UpdateUndoBtns();
#endregion apply to history
//apply settings
if (i < tifFI.sizeC & i == tifFI.cValue)
{
IA.RoiMan.current = null;
for (int j = 0; j < ColorBtnList.Count - 1; j++)
{
if (ColorBtnList[j].ImageIndex == 0)
{
tifFI.cValue = j;
break;
}
}
}
}
else
{
ctr.ImageIndex = 0;
#region apply to history
TifFileInfo fi = IA.TabPages.TabCollections[IA.TabPages.SelectedIndex].tifFI;
fi.delHist = true;
IA.delHist = true;
IA.UnDoBtn.Enabled = true;
IA.DeleteFromHistory();
fi.History.Add("enableColorChanel("
+ ColorBtnList.IndexOf(ctr).ToString() + ",false)");
fi.History.Add("enableColorChanel("
+ ColorBtnList.IndexOf(ctr).ToString() + ",true)");
IA.UpdateUndoBtns();
#endregion apply to history
if (ColorBtnList[tifFI.cValue].ImageIndex != 0)
{
for (int j = 0; j < ColorBtnList.Count - 1; j++)
{
if (ColorBtnList[j].ImageIndex == 0)
{
IA.RoiMan.current = null;
tifFI.cValue = j;
break;
}
}
}
}
IA.ReloadImages();
}
else if (e.Button == MouseButtons.Right)
{
if (i >= tifFI.LutList.Count)
{
return;
}
ColorDialog colorDialog1 = new ColorDialog();
colorDialog1.AllowFullOpen = true;
colorDialog1.AnyColor = true;
colorDialog1.FullOpen = true;
colorDialog1.Color = tifFI.LutList[i];
//set Custom Colors
if (IA.settings.CustomColors[IA.FileBrowser.ActiveAccountIndex] != "@")
{
List <int> colorsList = new List <int>();
foreach (string j in IA.settings.CustomColors[IA.FileBrowser.ActiveAccountIndex]
.Split(new[] { "\t" }, StringSplitOptions.None))
{
colorsList.Add(int.Parse(j));
}
colorDialog1.CustomColors = colorsList.ToArray();
}
// Show the color dialog.
DialogResult result = colorDialog1.ShowDialog();
//Copy Custom Colors
int[] colors = (int[])colorDialog1.CustomColors.Clone();
string txt = "@";
if (colors.Length > 0)
{
txt = colors[0].ToString();
for (int j = 1; j < colors.Length; j++)
{
txt += "\t" + colors[j].ToString();
}
}
IA.settings.CustomColors[IA.FileBrowser.ActiveAccountIndex] = txt;
IA.settings.Save();
if (result == DialogResult.OK)
{
IA.Input.ChangeValueFunction("LUT(" + i.ToString() + "," +
ColorTranslator.ToHtml(colorDialog1.Color).ToString() + ")");
}
}
}
public void ApplyCT3Tags(string[] BigVals, TifFileInfo fi)
{
int ind = LibTB.SelectedIndex;
LibTB.SuspendLayout();
string[] newBigVals = new string[BigVals.Length - 1];
Array.Copy(BigVals, 1, newBigVals, 0, newBigVals.Length);
string[] vals = BigVals[0].Split(new string[] { "\t" }, StringSplitOptions.None);
titleTB.Text = vals[0];
FiltersCB.Checked = bool.Parse(vals[1]);
SegmentationCB.Checked = bool.Parse(vals[2]);
SpotDetCB.Checked = bool.Parse(vals[3]);
TrackingCB.Checked = bool.Parse(vals[4]);
int sizeC = int.Parse(vals[5]);
if (vals.Length > 6)
{
ChartAxisCB.Checked = bool.Parse(vals[6]);
}
else
{
ChartAxisCB.Checked = false;
}
if (vals.Length > 7)
{
TimeStepCB.Checked = bool.Parse(vals[7]);
}
else
{
TimeStepCB.Checked = false;
}
if (sizeC == fi.sizeC)
{
fi.available = false;
fi.watershedList.Clear();
fi.tempWatershedList.Clear();
fi.FilterHistory.Clear();
fi.image16bitFilter = fi.image16bit;
fi.image8bitFilter = fi.image8bit;
fi.newFilterHistory = null;
fi.tempNewFilterHistory = null;
//Apply settings
int[] filters = IA.TabPages.myFileDecoder.ApplyCT3Tags(newBigVals, fi);
//background worker
var bgw = new BackgroundWorker();
bgw.WorkerReportsProgress = true;
//Add event for projection here
bgw.DoWork += new DoWorkEventHandler(delegate(Object o, DoWorkEventArgs a)
{
if (fi.tempNewFilterHistory == null || fi.tempNewFilterHistory.Length != fi.sizeC)
{
fi.isBinary = new bool[fi.sizeC];
fi.tempNewFilterHistory = new List <string> [fi.sizeC];
for (int i = 0; i < fi.sizeC; i++)
{
fi.tempNewFilterHistory[i] = new List <string>();
fi.isBinary[i] = false;
}
}
if (filters != null)
{
foreach (int i in filters)
{
for (int C = 0; C < fi.sizeC; C++)
{
string str = IA.Segmentation.MyFilters.DecodeOldFilters(C, i);
fi.tempNewFilterHistory[C].Add(str);
}
}
}
if (fi.tempWatershedList != null)
{
foreach (string val in fi.tempWatershedList)
{
int C = int.Parse(val.Split(new string[] { "\t" }, StringSplitOptions.None)[0]);
fi.tempNewFilterHistory[C].Add("wshed\t" + val);
}
}
fi.tempWatershedList.Clear();
//new filters
if (fi.tempNewFilterHistory != null)
{
try
{
foreach (List <string> commands in fi.tempNewFilterHistory)
{
if (commands != null)
{
foreach (string command in commands)
{
IA.Segmentation.MyFilters.FilterFromString(command, fi);
}
}
}
fi.newFilterHistory = fi.tempNewFilterHistory;
for (int i = 0; i < fi.sizeC; i++)
{
if (fi.newFilterHistory[i] == null)
{
fi.newFilterHistory[i] = new List <string>();
}
}
fi.tempNewFilterHistory = null;
}
catch { }
}
((BackgroundWorker)o).ReportProgress(0);
});
bgw.ProgressChanged += new ProgressChangedEventHandler(delegate(Object o, ProgressChangedEventArgs a)
{
IA.ReloadImages();
fi.available = true;
IA.FileBrowser.StatusLabel.Text = "Ready";
Loading = true;
LibTB.SelectedIndex = ind;
LibTB.ResumeLayout();
Loading = false;
});
//Start background worker
IA.FileBrowser.StatusLabel.Text = "Loading settings...";
//start bgw
bgw.RunWorkerAsync();
}
else
{
MessageBox.Show("Selected settings can be applied only on image with " + sizeC + " chanels!");
}
}
public static bool OpenFile(List <TabPage> Collection, string path, TabPage tp, ImageAnalyser IA, ToolStripStatusLabel StatusLabel)
{
StatusLabel.Text = "Reading Metadata...";
// read metadata using ImageReader
loci.formats.ImageReader FirstReader = new loci.formats.ImageReader();
try
{
FirstReader.setId(OSStringConverter.StringToDir(path));
}
catch
{
FirstReader.close();
StatusLabel.Text = "Ready";
return(false);
}
bool isRGB = FirstReader.isRGB();
//check is it rgb colored
loci.formats.ChannelSeparator reader = loci.formats.ChannelSeparator.makeChannelSeparator(FirstReader);
FirstReader = null;
TifFileInfo fi = tp.tifFI;
fi.seriesCount = reader.getSeriesCount();
//Select which series to open!!!!!
int ser = SelectSeries(reader, IA.TabPages.FileBrowser.StatusLabel);
if (ser == -1)
{
fi = null;
reader.close();
StatusLabel.Text = "Ready";
return(false);
}
else
{
reader.setSeries(ser);
}
//Check file bits per pixel - currently supported: 8 bit GrayScale, 16 bit GrayScale
fi.bitsPerPixel = reader.getBitsPerPixel();
if (fi.bitsPerPixel <= 8)
{
fi.bitsPerPixel = 8;
}
else if (fi.bitsPerPixel <= 16)
{
fi.bitsPerPixel = 16;
}
else
{
fi = null;
reader.close();
StatusLabel.Text = "Ready";
return(false);
}
//Check is the metadata complieted and return message if not
/*
* if (reader.isMetadataComplete() == false)
* {
* MessageBox.Show("Metadata is not complete!");
* }
*/
//read tags
fi.imageCount = reader.getImageCount();
fi.sizeX = reader.getSizeX();
fi.sizeY = reader.getSizeY();
fi.sizeZ = reader.getSizeZ();
fi.sizeC = reader.getSizeC();
fi.sizeT = reader.getSizeT();
//fi.dimensionOrder = reader.getDimensionOrder();
fi.dimensionOrder = "XYCZT";
fi.umZ = 0;
fi.umXY = 0;
fi.pixelType = reader.getPixelType();
fi.FalseColored = reader.isFalseColor();
fi.isIndexed = reader.isIndexed();
fi.MetadataComplete = reader.isMetadataComplete();
fi.DatasetStructureDescription = reader.getDatasetStructureDescription();
string description = getLibTifFileDescription(path);
fi.FileDescription = string.Join("\n", new string[] { "-----------------", "CoreMetadata:\n", reader.getCoreMetadataList().ToString(),
"-----------------", "GlobalMetadata:\n", reader.getGlobalMetadata().ToString(),
"-----------------", "SeriesMetadata:\n", reader.getSeriesMetadata().ToString(),
"-----------------", "FileDescription:\n", description });
//Apply def settings
fi.Dir = path;
fi.xAxisTB = IA.chart.Properties.xAxisTB.SelectedIndex;
fi.yAxisTB = IA.chart.Properties.yAxisTB.SelectedIndex;
fi.available = false;
fi.original = false;
//Create LUT table
if (isRGB)
{
fi.LutList = new List <Color>()
{
Color.FromArgb(255, 255, 0, 0),
Color.FromArgb(255, 0, 255, 0),
Color.FromArgb(255, 0, 0, 255)
};
}
else
{
fi.LutList = new List <Color>();
for (int i = 0; i < fi.sizeC; i++)
{
fi.LutList.Add(Color.White);
}
}
//Create time steps table
fi.TimeSteps = new List <double>();
fi.TimeSteps.Add(fi.imageCount + 1);
fi.TimeSteps.Add(1);
//If its IQ3 format or Dragonflye - try to read the colors and the timesteps
TryAndorDecoders(fi, description);
if (fi.sizeC == 0)
{
fi.sizeC = 1;
}
if (fi.sizeZ == 0)
{
fi.sizeZ = 1;
}
if (fi.sizeT == 0)
{
fi.sizeT = 1;
}
#region Segmentation variables
fi.SegmentationCBoxIndex = new int[fi.sizeC];
fi.SegmentationProtocol = new int[fi.sizeC];
fi.thresholdsCBoxIndex = new int[fi.sizeC];
fi.sumHistogramChecked = new bool[fi.sizeC];
fi.thresholdValues = new int[fi.sizeC][];
fi.thresholdColors = new Color[fi.sizeC][];
fi.RefThresholdColors = new Color[fi.sizeC][];
fi.thresholds = new int[fi.sizeC];
fi.SpotColor = new Color[fi.sizeC];
fi.RefSpotColor = new Color[fi.sizeC];
fi.SelectedSpotThresh = new int[fi.sizeC];
fi.SpotThresh = new int[fi.sizeC];
fi.typeSpotThresh = new int[fi.sizeC];
fi.SpotTailType = new string[fi.sizeC];
fi.spotSensitivity = new int[fi.sizeC];
fi.roiList = new List <ROI> [fi.sizeC];
fi.tracking_MaxSize = new int[fi.sizeC];
fi.tracking_MinSize = new int[fi.sizeC];
fi.tracking_Speed = new int[fi.sizeC];
for (int i = 0; i < fi.sizeC; i++)
{
fi.sumHistogramChecked[i] = false;
fi.thresholdValues[i] = new int[5];
fi.thresholdColors[i] = new Color[]
{ Color.Transparent, Color.Transparent, Color.Transparent, Color.Transparent, Color.Transparent };
fi.RefThresholdColors[i] = new Color[]
{ Color.Black, Color.Orange, Color.Green, Color.Blue, Color.Magenta };
fi.SpotColor[i] = Color.Red;
fi.RefSpotColor[i] = Color.Red;
fi.SpotTailType[i] = "<";
fi.spotSensitivity[i] = 100;
fi.tracking_MaxSize[i] = 10000;
fi.tracking_MinSize[i] = 5;
fi.tracking_Speed[i] = 5;
}
#endregion Segmentation variables
//background worker
var bgw = new BackgroundWorker();
bgw.WorkerReportsProgress = true;
bool loaded = false;
//Add handlers to the backgroundworker
bgw.DoWork += new DoWorkEventHandler(delegate(Object o, DoWorkEventArgs a)
{
//prepare array and read file
if (isLibTifCompatible(path) && !isRGB)
{
int[] dimOrder = GetFrameIndexes(reader, fi);
Tiff image = Tiff.Open(OSStringConverter.StringToDir(path), "r");
//prepare array and read file
int midFrame = fi.sizeC * fi.sizeZ;
switch (fi.bitsPerPixel)
{
case 8:
fi.image8bit = new byte[image.NumberOfDirectories()][][];
for (int i = 0; i < midFrame; i++)
{
if (i >= fi.imageCount)
{
break;
}
IA.TabPages.myFileDecoder.Image8bit_readFrame(i, image, fi, dimOrder);
}
break;
case 16:
fi.image16bit = new ushort[image.NumberOfDirectories()][][];
for (int i = 0; i < midFrame; i++)
{
if (i >= fi.imageCount)
{
break;
}
IA.TabPages.myFileDecoder.Image16bit_readFrame(i, image, fi, dimOrder);
}
break;
}
loaded = true;
//report progress
((BackgroundWorker)o).ReportProgress(0);
//parallel readers
IA.TabPages.myFileDecoder.ImageReader_BGW(Collection, image, fi, IA, path, dimOrder);
//report progress
dimOrder = null;
image.Close();
((BackgroundWorker)o).ReportProgress(1);
}
else
{
//Read the first T
byte[] buf;
switch (fi.bitsPerPixel)
{
case 8:
fi.image8bit = new byte[fi.imageCount][][];
buf = new byte[fi.sizeX * fi.sizeY];
for (int z = 0, i = 0; z < fi.sizeZ; z++)
{
for (int c = 0; c < fi.sizeC; c++, i++)
{
fi.image8bit[i] = Image8bit_readFrame(
reader.openBytes(
reader.getIndex(z, c, 0), buf),
fi.sizeX, fi.sizeY);
//fi.LutList[c] = ReadLut(reader);
}
}
break;
case 16:
fi.image16bit = new ushort[fi.imageCount][][];
buf = new byte[fi.sizeX * fi.sizeY * 2];
for (int z = 0, i = 0; z < fi.sizeZ; z++)
{
for (int c = 0; c < fi.sizeC; c++, i++)
{
fi.image16bit[i] = Image16bit_readFrame(
reader.openBytes(
reader.getIndex(z, c, 0), buf),
fi.sizeX, fi.sizeY);
//fi.LutList[c] = ReadLut(reader);
}
}
break;
}
loaded = true;
//report progress
((BackgroundWorker)o).ReportProgress(0);
//Read the rest T
byte[][] bigBuf = new byte[fi.imageCount][];
int ZCcount = fi.sizeC * fi.sizeZ;
for (int t = 1, i = ZCcount; t < fi.sizeT; t++)
{
for (int z = 0; z < fi.sizeZ; z++)
{
for (int c = 0; c < fi.sizeC; c++, i++)
{
if (fi.image8bit != null || fi.image16bit != null)
{
bigBuf[i] = reader.openBytes(reader.getIndex(z, c, t));
}
}
}
}
//Format the arrays for CellTool
Parallel.For(ZCcount, fi.imageCount, (int i) =>
{
try
{
switch (fi.bitsPerPixel)
{
case 8:
if (fi.image8bit != null)
{
fi.image8bit[i] = Image8bit_readFrame(bigBuf[i],
fi.sizeX, fi.sizeY);
}
bigBuf[i] = null;
break;
case 16:
if (fi.image16bit != null)
{
fi.image16bit[i] = Image16bit_readFrame(bigBuf[i],
fi.sizeX, fi.sizeY);
}
bigBuf[i] = null;
break;
}
}
catch { }
});
bigBuf = null;
//report progress
((BackgroundWorker)o).ReportProgress(1);
}
});
bgw.ProgressChanged += new ProgressChangedEventHandler(delegate(Object o, ProgressChangedEventArgs a)
{
if (a.ProgressPercentage == 0)
{
fi.openedImages = fi.sizeC * fi.sizeZ;
try
{
IA.ReloadImages();
}
catch { }
}
else if (a.ProgressPercentage == 1)
{
//dispose the reader
reader.close();
reader = null;
//mark as loaded
fi.openedImages = fi.imageCount;
fi.available = true;
fi.loaded = true;
IA.TabPages.myFileDecoder.CalculateAllRois(fi);
IA.ReloadImages();
bool check = true;
foreach (TabPage tp1 in Collection)
{
if (tp1.tifFI != null && tp1.tifFI.available == false)
{
check = false;
break;
}
}
if (check == true)
{
IA.TabPages.myFileDecoder.loadingTimer.Stop();
StatusLabel.Text = "Ready";
}
if (IA.Segmentation.AutoSetUp.LibTB.SelectedIndex > 0 &&
IA.Segmentation.AutoSetUp.ApplyToNewCheckB.Checked &&
MessageBox.Show("Do you want to open the image with the following protocol:\n" +
IA.Segmentation.AutoSetUp.LibTB.Text,
"", MessageBoxButtons.YesNo) == System.Windows.Forms.DialogResult.Yes)
{
IA.Segmentation.AutoSetUp.ApplyCT3Tags(IA.Segmentation.AutoSetUp.protocols[
IA.Segmentation.AutoSetUp.LibTB.SelectedIndex].Split(
new string[] { ";\n" }, StringSplitOptions.None), fi);
}
}
});
//Start background worker
StatusLabel.Text = "Reading Image...";
//Clear OldImage
IA.IDrawer.ClearImage();
//start bgw
bgw.RunWorkerAsync();
//add taskbar
tp.tifFI.tpTaskbar.Initialize(IA.TabPages.ImageMainPanel, IA, fi);
try
{
if (loaded == true)
{
IA.ReloadImages();
}
}
catch { }
//out put
return(true);
}
private void Node_Click(object sender, TreeNodeMouseClickEventArgs e)
{
if (MouseButtons.Right != e.Button)
{
return;
}
tv.SelectedNode = e.Node;
TreeNode n = e.Node;
if (n == null || n.Tag == null)
{
return;
}
ROI roi = (ROI)n.Tag;
if (roi == null)
{
roi = (ROI)n.Parent.Tag;
}
int ind = 0;
if (n.Text.IndexOf("Layer") > -1)
{
ind = int.Parse(n.Text.Substring(n.Text.LastIndexOf("r") + 1, n.Text.Length - n.Text.LastIndexOf("r") - 1));
}
//ColorBrowser
ColorDialog colorDialog1 = new ColorDialog();
colorDialog1.AllowFullOpen = true;
colorDialog1.AnyColor = true;
colorDialog1.FullOpen = true;
colorDialog1.Color = roi.colors[ind];
//set Custom Colors
if (IA.settings.CustomColors[IA.FileBrowser.ActiveAccountIndex] != "@")
{
List <int> colorsList = new List <int>();
foreach (string j in IA.settings.CustomColors[IA.FileBrowser.ActiveAccountIndex]
.Split(new[] { "\t" }, StringSplitOptions.None))
{
colorsList.Add(int.Parse(j));
}
colorDialog1.CustomColors = colorsList.ToArray();
}
// Show the color dialog.
DialogResult result = colorDialog1.ShowDialog();
//Copy Custom Colors
int[] colors = (int[])colorDialog1.CustomColors.Clone();
string txt = "@";
if (colors.Length > 0)
{
txt = colors[0].ToString();
for (int j = 1; j < colors.Length; j++)
{
txt += "\t" + colors[j].ToString();
}
}
IA.settings.CustomColors[IA.FileBrowser.ActiveAccountIndex] = txt;
IA.settings.Save();
if (result == DialogResult.OK)
{
IA.AddToHistoryOld("Chart.SetSeriesColor(" + GetChanel(roi).ToString() + ","
+ roi.getID.ToString() + "," + ind.ToString() + "," + ColorTranslator.ToHtml(roi.colors[ind]) + ")");
roi.colors[ind] = colorDialog1.Color;
IA.AddToHistoryNew("Chart.SetSeriesColor(" + GetChanel(roi).ToString() + ","
+ roi.getID.ToString() + "," + ind.ToString() + "," + ColorTranslator.ToHtml(colorDialog1.Color) + ")");
foreach (Color col in RefColors)
{
if (colorDialog1.Color == col)
{
IA.ReloadImages();
return;
}
}
RefColors.Add(colorDialog1.Color);
createImagesFortTV(colorDialog1.Color);
IA.ReloadImages();
}
}
private void createDialog()
{
Dialog.FormBorderStyle = FormBorderStyle.FixedDialog;
Dialog.Text = "Watershed";
Dialog.StartPosition = FormStartPosition.CenterScreen;
Dialog.WindowState = FormWindowState.Normal;
Dialog.MinimizeBox = false;
Dialog.MaximizeBox = false;
Dialog.BackColor = IA.FileBrowser.BackGround2Color1;
Dialog.ForeColor = IA.FileBrowser.ShriftColor1;
Dialog.FormClosing += new FormClosingEventHandler(delegate(object o, FormClosingEventArgs a)
{
Dialog.Visible = false;
a.Cancel = true;
});
Dialog.Width = 240;
Dialog.Height = 190;
Dialog.SuspendLayout();
/*
* Label ThresholdLab = new Label();
* ThresholdLab.Text = "Select threshold:";
* ThresholdLab.Width = 100;
* ThresholdLab.Location = new System.Drawing.Point(10,15);
* Dialog.Controls.Add(ThresholdLab);
*
* ThresholdCB.Width = 30;
* ThresholdCB.Location = new System.Drawing.Point(110, 13);
* Dialog.Controls.Add(ThresholdCB);
* ThresholdCB.SelectedIndexChanged += new EventHandler(delegate (object o, EventArgs a)
* {
* threshold = ThresholdCB.SelectedIndex + 1;
* });
*/
Label ToleranceLab = new Label();
ToleranceLab.Text = "Tolerance:";
ToleranceLab.Width = 100;
ToleranceLab.Location = new System.Drawing.Point(10, 15);
Dialog.Controls.Add(ToleranceLab);
ToleranceTB.Text = tolerance.ToString();
ToleranceTB.Width = 30;
ToleranceTB.Location = new System.Drawing.Point(110, 13);
Dialog.Controls.Add(ToleranceTB);
Label DistanceLab = new Label();
DistanceLab.Text = "Distance Transform:";
DistanceLab.Width = 100;
DistanceLab.Location = new System.Drawing.Point(10, 45);
Dialog.Controls.Add(DistanceLab);
ComboBox DistanceCB = new ComboBox();
DistanceCB.Width = 110;
DistanceCB.Items.AddRange(new string[] { "Euclidean", "SquaredEuclidean", "Chessboard", "Manhattan" });
DistanceCB.SelectedIndex = 0;
DistanceCB.SelectedIndexChanged += new EventHandler(delegate(object o, EventArgs a)
{
switch (DistanceCB.SelectedIndex)
{
case 0:
distance = DistanceTransformMethod.Euclidean;
break;
case 1:
distance = DistanceTransformMethod.SquaredEuclidean;
break;
case 2:
distance = DistanceTransformMethod.Chessboard;
break;
case 3:
distance = DistanceTransformMethod.Manhattan;
break;
default:
distance = DistanceTransformMethod.Euclidean;
break;
}
});
DistanceCB.Location = new System.Drawing.Point(110, 43);
Dialog.Controls.Add(DistanceCB);
FillHolesCB.Text = "Fill holes";
FillHolesCB.Location = new System.Drawing.Point(15, 75);
Dialog.Controls.Add(FillHolesCB);
//buttons
Panel okBox = new Panel();
okBox.Height = 40;
okBox.Dock = DockStyle.Bottom;
Dialog.Controls.Add(okBox);
Button okBtn = new Button();
okBtn.Text = "Process";
okBtn.BackColor = System.Drawing.SystemColors.ButtonFace;
okBtn.ForeColor = System.Drawing.Color.Black;
okBtn.Location = new System.Drawing.Point(20, 10);
okBtn.Anchor = AnchorStyles.Top | AnchorStyles.Left;
okBox.Controls.Add(okBtn);
Button cancelBtn = new Button();
cancelBtn.Text = "Cancel";
cancelBtn.BackColor = System.Drawing.SystemColors.ButtonFace;
cancelBtn.Location = new System.Drawing.Point(Dialog.Width - cancelBtn.Width - 60, 10);
cancelBtn.ForeColor = System.Drawing.Color.Black;
cancelBtn.Anchor = AnchorStyles.Top | AnchorStyles.Right;
okBox.Controls.Add(cancelBtn);
okBtn.Click += new EventHandler(delegate(object sender, EventArgs e)
{
try
{
tolerance = float.Parse(ToleranceTB.Text);
}
catch
{
MessageBox.Show("Tolerance must be numeric!");
return;
}
Dialog.Visible = false;
//event
TifFileInfo fi = null;
try
{
fi = IA.TabPages.TabCollections[IA.TabPages.SelectedIndex].tifFI;
}
catch { return; }
if (fi == null)
{
return;
}
if (!fi.available)
{
MessageBox.Show("Image is not ready yet! \nTry again later.");
return;
}
int C = fi.cValue;
//background worker
var bgw = new BackgroundWorker();
bgw.WorkerReportsProgress = true;
fi.available = false;
fi.fillHoles = FillHolesCB.Checked;
//Add event for projection here
bgw.DoWork += new DoWorkEventHandler(delegate(Object o, DoWorkEventArgs a)
{
ProcessFilter(fi, C, this.tolerance, 1, this.distance);
((BackgroundWorker)o).ReportProgress(0);
});
bgw.ProgressChanged += new ProgressChangedEventHandler(delegate(Object o, ProgressChangedEventArgs a)
{
if (a.ProgressPercentage == 0)
{
IA.Segmentation.MyFilters.addToHistoryOldInfo(C, fi);
fi.newFilterHistory[C].Add(
ToString(fi, C, this.tolerance, 1, this.distance));
IA.Segmentation.MyFilters.addToHistoryNewInfo(C, fi);
IA.MarkAsNotSaved();
IA.ReloadImages();
}
fi.available = true;
IA.FileBrowser.StatusLabel.Text = "Ready";
});
//Start background worker
IA.FileBrowser.StatusLabel.Text = "Watershed...";
//start bgw
bgw.RunWorkerAsync();
});
cancelBtn.Click += new EventHandler(delegate(object sender, EventArgs e)
{
Dialog.Visible = false;
});
Dialog.KeyPreview = true;
Dialog.KeyDown += new KeyEventHandler(delegate(object sender, KeyEventArgs e)
{
switch (e.KeyCode)
{
case Keys.Escape:
Dialog.Visible = false;
break;
case Keys.Enter:
okBtn.PerformClick();
break;
default:
break;
}
});
Dialog.ResumeLayout();
}
private static bool SubstackToolStripMenuItem_click(string path, List <TabPage> Collection, TabPage tp, ImageAnalyser IA, loci.formats.ChannelSeparator reader, int[][] dim)
{
TifFileInfo fi = tp.tifFI;
if (dim == null)
{
return(false);
}
////////
/////background worker
bool loaded = false;
//Add handlers to the backgroundworker
var bgw = new BackgroundWorker();
bgw.WorkerReportsProgress = true;
TifFileInfo newFI = null;
//Add event for projection here
bgw.DoWork += new DoWorkEventHandler(delegate(Object o, DoWorkEventArgs a)
{
//Prepare matrix
int T, Z, C, f = 0,
curT, curZ, curC;
List <int> matrix = new List <int>();
for (T = 0, curT = dim[0][0] - 1; T < fi.sizeT; T++)
{
if (T == curT && T < dim[0][1])
{
for (Z = 0, curZ = dim[1][0] - 1; Z < fi.sizeZ; Z++)
{
if (Z == curZ && Z < dim[1][1])
{
for (C = 0, curC = dim[2][0] - 1; C < fi.sizeC; C++, f++)
{
if (C == curC && C < dim[2][1])
{
//matrix.Add(f);
matrix.Add(reader.getIndex(Z, C, T));
curC += dim[2][2];
}
}
curZ += dim[1][2];
}
else
{
f += fi.sizeC;
}
}
curT += dim[0][2];
}
else
{
f += fi.sizeC * fi.sizeZ;
}
}
newFI = fi;
newFI.Dir = newFI.Dir.Substring(0, newFI.Dir.LastIndexOf(".")) +
"_Substack.tif";
newFI.sizeT = 0;
for (int i = dim[0][0] - 1; i < dim[0][1]; i += dim[0][2])
{
newFI.sizeT++;
}
newFI.sizeZ = 0;
for (int i = dim[1][0] - 1; i < dim[1][1]; i += dim[1][2])
{
newFI.sizeZ++;
}
newFI.sizeC = 0;
List <Color> colList = new List <Color>();
for (int i = dim[2][0] - 1; i < dim[2][1]; i += dim[2][2])
{
newFI.sizeC++;
colList.Add(fi.LutList[i]);
}
newFI.imageCount = matrix.Count;
newFI.openedImages = matrix.Count;
AddEmptyArraysToFI(newFI);
newFI.LutList = colList;
//read image
//Read the first T
//prepare array and read file
if (isLibTifCompatible(path) && !reader.isRGB())
{
int[] dimOrder = matrix.ToArray();
Tiff image = Tiff.Open(path, "r");
//prepare array and read file
int midFrame = fi.sizeC * fi.sizeZ;
switch (fi.bitsPerPixel)
{
case 8:
fi.image8bit = new byte[fi.imageCount][][];
for (int i = 0; i < midFrame; i++)
{
if (i >= fi.imageCount)
{
break;
}
IA.TabPages.myFileDecoder.Image8bit_readFrame(i, image, fi, dimOrder);
}
break;
case 16:
fi.image16bit = new ushort[fi.imageCount][][];
for (int i = 0; i < midFrame; i++)
{
if (i >= fi.imageCount)
{
break;
}
IA.TabPages.myFileDecoder.Image16bit_readFrame(i, image, fi, dimOrder);
}
break;
}
loaded = true;
//report progress
((BackgroundWorker)o).ReportProgress(0);
//parallel readers
IA.TabPages.myFileDecoder.ImageReader_BGW(Collection, image, fi, IA, path, dimOrder);
//report progress
dimOrder = null;
image.Close();
((BackgroundWorker)o).ReportProgress(1);
}
else
{
byte[] buf;
switch (fi.bitsPerPixel)
{
case 8:
fi.image8bit = new byte[fi.imageCount][][];
buf = new byte[fi.sizeX * fi.sizeY];
for (int z = 0, i = 0; z < fi.sizeZ; z++)
{
for (int c = 0; c < fi.sizeC; c++, i++)
{
fi.image8bit[i] = Image8bit_readFrame(
reader.openBytes(
matrix[i], buf),
fi.sizeX, fi.sizeY);
//fi.LutList[c] = ReadLut(reader);
}
}
break;
case 16:
fi.image16bit = new ushort[fi.imageCount][][];
buf = new byte[fi.sizeX * fi.sizeY * 2];
for (int z = 0, i = 0; z < fi.sizeZ; z++)
{
for (int c = 0; c < fi.sizeC; c++, i++)
{
fi.image16bit[i] = Image16bit_readFrame(
reader.openBytes(
matrix[i], buf),
fi.sizeX, fi.sizeY);
//fi.LutList[c] = ReadLut(reader);
}
}
break;
}
loaded = true;
//report progress
((BackgroundWorker)o).ReportProgress(0);
//Read the rest T
byte[][] bigBuf = new byte[fi.imageCount][];
int ZCcount = fi.sizeC * fi.sizeZ;
for (int t = 1, i = ZCcount; t < fi.sizeT; t++)
{
for (int z = 0; z < fi.sizeZ; z++)
{
for (int c = 0; c < fi.sizeC; c++, i++)
{
if (fi.image8bit != null || fi.image16bit != null)
{
bigBuf[i] = reader.openBytes(matrix[i]);
}
}
}
}
//Format the arrays for CellTool
Parallel.For(ZCcount, fi.imageCount, (int i) =>
{
try
{
switch (fi.bitsPerPixel)
{
case 8:
if (fi.image8bit != null)
{
fi.image8bit[i] = Image8bit_readFrame(bigBuf[i],
fi.sizeX, fi.sizeY);
}
bigBuf[i] = null;
break;
case 16:
if (fi.image16bit != null)
{
fi.image16bit[i] = Image16bit_readFrame(bigBuf[i],
fi.sizeX, fi.sizeY);
}
bigBuf[i] = null;
break;
}
}
catch { }
});
bigBuf = null;
//report progress
((BackgroundWorker)o).ReportProgress(1);
}
});
bgw.ProgressChanged += new ProgressChangedEventHandler(delegate(Object o, ProgressChangedEventArgs a)
{
if (a.ProgressPercentage == 0)
{
fi.openedImages = fi.sizeC * fi.sizeZ;
try
{
IA.ReloadImages();
}
catch { }
}
else if (a.ProgressPercentage == 1)
{
//dispose the reader
reader.close();
reader = null;
//mark as loaded
fi.openedImages = fi.imageCount;
fi.available = true;
fi.loaded = true;
newFI.original = false;
IA.TabPages.myFileDecoder.CalculateAllRois(fi);
IA.ReloadImages();
bool check = true;
foreach (TabPage tp1 in Collection)
{
if (tp1.tifFI != null && tp1.tifFI.available == false)
{
check = false;
break;
}
}
if (check == true)
{
IA.TabPages.myFileDecoder.loadingTimer.Stop();
IA.FileBrowser.StatusLabel.Text = "Ready";
}
if (IA.Segmentation.AutoSetUp.LibTB.SelectedIndex > 0 &&
IA.Segmentation.AutoSetUp.ApplyToNewCheckB.Checked &&
MessageBox.Show("Do you want to open the image with the following protocol:\n" +
IA.Segmentation.AutoSetUp.LibTB.Text,
"", MessageBoxButtons.YesNo) == System.Windows.Forms.DialogResult.Yes)
{
IA.Segmentation.AutoSetUp.ApplyCT3Tags(IA.Segmentation.AutoSetUp.protocols[
IA.Segmentation.AutoSetUp.LibTB.SelectedIndex].Split(
new string[] { ";\n" }, StringSplitOptions.None), fi);
}
}
});
//Start background worker
IA.FileBrowser.StatusLabel.Text = "Reading Image...";
//Clear OldImage
IA.IDrawer.ClearImage();
//start bgw
bgw.RunWorkerAsync();
//add taskbar
tp.tifFI.tpTaskbar.Initialize(IA.TabPages.ImageMainPanel, IA, fi);
try
{
if (loaded == true)
{
IA.ReloadImages();
}
}
catch { }
//output
return(true);
}
private void ColBtn_Click(object sender, MouseEventArgs e)
{
TifFileInfo fi = IA.TabPages.TabCollections[IA.TabPages.SelectedIndex].tifFI;
Button btn = (Button)sender;
if (e.Button == MouseButtons.Left)
{
//disable/enable color
if (fi.SpotColor[fi.cValue] == Color.Transparent)
{
fi.SpotColor[fi.cValue] = fi.RefSpotColor[fi.cValue];
}
else
{
fi.SpotColor[fi.cValue] = Color.Transparent;
}
#region apply to history
//segmentation.SpotDetector(chanelN,ThreshVal,HTML color, tail type, thresh type)
Color val = fi.RefSpotColor[fi.cValue];
Color oldVal = Color.Transparent;
if (fi.SpotColor[fi.cValue] == Color.Transparent)
{
val = Color.Transparent; oldVal = fi.RefSpotColor[fi.cValue];
}
fi.delHist = true;
IA.delHist = true;
IA.UnDoBtn.Enabled = true;
IA.DeleteFromHistory();
fi.History.Add("segmentation.SpotDetector("
+ fi.cValue.ToString() + "," +
fi.SpotThresh[fi.cValue].ToString() + "," +
fi.spotSensitivity[fi.cValue].ToString() + "," +
ColorTranslator.ToHtml(oldVal) + "," +
fi.SpotTailType[fi.cValue] + "," +
fi.SelectedSpotThresh[fi.cValue].ToString() + "," +
fi.typeSpotThresh[fi.cValue].ToString() + ")");
fi.History.Add("segmentation.SpotDetector("
+ fi.cValue.ToString() + "," +
fi.SpotThresh[fi.cValue].ToString() + "," +
fi.spotSensitivity[fi.cValue].ToString() + "," +
ColorTranslator.ToHtml(val) + "," +
fi.SpotTailType[fi.cValue] + "," +
fi.SelectedSpotThresh[fi.cValue].ToString() + "," +
fi.typeSpotThresh[fi.cValue].ToString() + ")");
IA.UpdateUndoBtns();
IA.MarkAsNotSaved();
#endregion apply to history
IA.ReloadImages();
}
else if (e.Button == MouseButtons.Right & btn.Text == "")
{
//change color
ColorDialog colorDialog1 = new ColorDialog();
colorDialog1.AllowFullOpen = true;
colorDialog1.AnyColor = true;
colorDialog1.FullOpen = true;
colorDialog1.Color = fi.RefSpotColor[fi.cValue];
//set Custom Colors
if (IA.settings.CustomColors[IA.FileBrowser.ActiveAccountIndex] != "@")
{
List <int> colorsList = new List <int>();
foreach (string j in IA.settings.CustomColors[IA.FileBrowser.ActiveAccountIndex]
.Split(new[] { "\t" }, StringSplitOptions.None))
{
colorsList.Add(int.Parse(j));
}
colorDialog1.CustomColors = colorsList.ToArray();
}
// Show the color dialog.
DialogResult result = colorDialog1.ShowDialog();
//Copy Custom Colors
int[] colors = (int[])colorDialog1.CustomColors.Clone();
string txt = "@";
if (colors.Length > 0)
{
txt = colors[0].ToString();
for (int j = 1; j < colors.Length; j++)
{
txt += "\t" + colors[j].ToString();
}
}
IA.settings.CustomColors[IA.FileBrowser.ActiveAccountIndex] = txt;
IA.settings.Save();
if (result == DialogResult.OK)
{
#region apply to history
//segmentation.SpotDetector(chanelN,ThreshVal,HTML color, tail type, thresh type)
fi.delHist = true;
IA.delHist = true;
IA.UnDoBtn.Enabled = true;
IA.DeleteFromHistory();
fi.History.Add("segmentation.SpotDetector("
+ fi.cValue.ToString() + "," +
fi.SpotThresh[fi.cValue].ToString() + "," +
fi.spotSensitivity[fi.cValue].ToString() + "," +
ColorTranslator.ToHtml(fi.RefSpotColor[fi.cValue]) + "," +
fi.SpotTailType[fi.cValue] + "," +
fi.SelectedSpotThresh[fi.cValue].ToString() + "," +
fi.typeSpotThresh[fi.cValue].ToString() + ")");
fi.History.Add("segmentation.SpotDetector("
+ fi.cValue.ToString() + "," +
fi.SpotThresh[fi.cValue].ToString() + "," +
fi.spotSensitivity[fi.cValue].ToString() + "," +
ColorTranslator.ToHtml(colorDialog1.Color) + "," +
fi.SpotTailType[fi.cValue] + "," +
fi.SelectedSpotThresh[fi.cValue].ToString() + "," +
fi.typeSpotThresh[fi.cValue].ToString() + ")");
IA.UpdateUndoBtns();
IA.MarkAsNotSaved();
#endregion apply to history
fi.RefSpotColor[fi.cValue] = colorDialog1.Color;
fi.SpotColor[fi.cValue] = colorDialog1.Color;
IA.ReloadImages();
}
}
}
private void PlugIn_Load(string path)
{
TifFileInfo oldFI = null;
try
{
oldFI = IA.TabPages.TabCollections[IA.TabPages.SelectedIndex].tifFI;
}
catch { }
CellToolDK.TifFileInfo fi = FItoCellToolDK(oldFI);
var DLL = Assembly.LoadFile(path);
foreach (Type type in DLL.GetExportedTypes())
{
try
{
Transmiter e = new Transmiter();
e.Changed += new TransmiterEventHandler(delegate(object o, TransmiterEventArgs a)
{
try
{
if (fi != null && oldFI != null)
{
if (fi.sizeC != oldFI.sizeC)
{
oldFI.sizeC = fi.sizeC;
FI_reduseC(fi);
FI_reduseC(oldFI);
}
CellToolDKtoFI(fi, oldFI);
if (oldFI.sizeZ > 1)
{
IA.TabPages.zTrackBar.Refresh(oldFI.zValue + 1, 1, oldFI.sizeZ);
IA.TabPages.zTrackBar.Panel.Visible = true;
}
else
{
IA.TabPages.zTrackBar.Panel.Visible = false;
}
if (oldFI.sizeT > 1)
{
IA.TabPages.tTrackBar.Refresh(oldFI.frame + 1, 1, oldFI.sizeT);
IA.TabPages.tTrackBar.Panel.Visible = true;
}
else
{
IA.TabPages.tTrackBar.Panel.Visible = false;
}
}
}
catch { MessageBox.Show("Error with reporting back!"); }
IA.ReloadImages();
});
var c = Activator.CreateInstance(type);
try
{
type.InvokeMember("Input", BindingFlags.InvokeMethod, null, c, new object[] { fi, e });
}
catch { MessageBox.Show("Input void is not avaliable!"); }
break;
}
catch { }
}
}