private void AddCurve(SrmTransition trans, double maxIntensity, string title, Color color)
{
if (trans == null || trans.Intensities == null || trans.Intensities.Count == 0)
{
return;
}
var item = trans.Intensities;
var ppl = new PointPairList();
item.ForEach(m => ppl.Add(m.RetentionTime, m.Intensity * 100 / maxIntensity));
panel.AddCurve(MyConvert.Format("{0}, {1:0.00}-{2:0.00}", title, item[0].PrecursorMz, item[0].ProductMz), ppl, color, SymbolType.None);
var pplCurve = new PointPairList();
bool bChecked = false;
item.ForEach(envelope =>
{
if (envelope.Enabled != bChecked)
{
if (pplCurve.Count > 0)
{
if (bChecked)
{
panel.AddPoly("", pplCurve, color, new[] { SilacQuantificationConstants.PLOY_COLOR });
}
}
pplCurve = new PointPairList();
bChecked = envelope.Enabled;
}
pplCurve.Add(envelope.RetentionTime, envelope.Intensity * 100 / maxIntensity);
});
if (pplCurve.Count > 0)
{
if (bChecked)
{
panel.AddPoly("", pplCurve, color, new[] { SilacQuantificationConstants.PLOY_COLOR });
}
}
}
private void UpdateCurve()
{
foreach (GraphPane pane in zgcCurve.MasterPane.PaneList)
{
pane.ClearData();
}
try
{
if (lvPrecursor.SelectedItems.Count == 0)
{
return;
}
GraphPane graphScans = zgcCurve.MasterPane.PaneList[0];
GraphPane graphPPM = zgcCurve.MasterPane.PaneList[1];
var pplScan = new PointPairList();
var pplPPM = new PointPairList();
var pplScanCurve = new PointPairList();
bool bChecked = false;
double precursorMz = MyConvert.ToDouble(lvPrecursor.SelectedItems[0].Text);
var identified = new PointPairList();
foreach (ListViewItem item in this.lvScans.Items)
{
if (item == null)
{
return;
}
if (item.Checked != bChecked)
{
if (pplScanCurve.Count > 0 && bChecked)
{
graphScans.AddPoly("", pplScanCurve, Color.Blue, new[] { PLOY_COLOR });
}
pplScanCurve = new PointPairList();
bChecked = item.Checked;
}
LabelFreeItem c = (LabelFreeItem)item.Tag;
pplScan.Add(new PointPair(c.Scan, c.AdjustIntensity));
pplScanCurve.Add(new PointPair(c.Scan, c.AdjustIntensity));
pplPPM.Add(new PointPair(c.Scan, c.DeltaMzPPM));
if (c.Identified)
{
identified.Add(new PointPair(c.Scan, c.AdjustIntensity));
}
}
if (pplScanCurve.Count > 0 && bChecked)
{
graphScans.AddPoly("", pplScanCurve, Color.Blue, new[] { PLOY_COLOR });
}
graphScans.AddCurve(precursorMz.ToString(), pplScan, Color.Blue, SymbolType.None);
graphPPM.AddCurve(precursorMz.ToString(), pplPPM, Color.Blue, SymbolType.None);
graphScans.AddIndividualLine("Identified", identified, Color.Red);
}
finally
{
ZedGraphicExtension.UpdateGraph(this.zgcCurve);
}
}
public void Update(object sender, UpdateQuantificationItemEventArgs e)
{
var summary = e.Item as SilacQuantificationSummaryItem;
if (summary == null)
{
throw new ArgumentNullException("UpdateQuantificationItemEventArgs.Item cannot be null");
}
double sampleMz;
double referenceMz;
if (summary.SampleIsLight)
{
sampleMz = summary.ObservedEnvelopes[0].Light[0].Mz;
referenceMz = summary.ObservedEnvelopes[0].Heavy[0].Mz;
}
else
{
sampleMz = summary.ObservedEnvelopes[0].Heavy[0].Mz;
referenceMz = summary.ObservedEnvelopes[0].Light[0].Mz;
}
panel.ClearData();
var pplSample = new PointPairList();
var pplReference = new PointPairList();
var pplSampleCurve = new PointPairList();
var pplReferenceCurve = new PointPairList();
bool bChecked = false;
for (int i = 0; i < summary.ObservedEnvelopes.Count; i++)
{
var envelope = summary.ObservedEnvelopes[i];
if (envelope.Enabled != bChecked)
{
if (pplSampleCurve.Count > 0)
{
if (bChecked)
{
panel.AddPoly("", pplSampleCurve, SilacQuantificationConstants.SAMPLE_COLOR, new[] { SilacQuantificationConstants.PLOY_COLOR });
panel.AddPoly("", pplReferenceCurve, SilacQuantificationConstants.REFERENCE_COLOR, new[] { SilacQuantificationConstants.PLOY_COLOR });
}
}
pplSampleCurve = new PointPairList();
pplReferenceCurve = new PointPairList();
bChecked = envelope.Enabled;
}
if (summary.SampleIsLight)
{
pplSampleCurve.Add(envelope.Scan, envelope.LightIntensity);
pplReferenceCurve.Add(envelope.Scan, envelope.HeavyIntensity);
pplSample.Add(envelope.Scan, envelope.LightIntensity);
pplReference.Add(envelope.Scan, envelope.HeavyIntensity);
}
else
{
pplSampleCurve.Add(envelope.Scan, envelope.LightIntensity);
pplReferenceCurve.Add(envelope.Scan, envelope.HeavyIntensity);
pplSample.Add(envelope.Scan, envelope.HeavyIntensity);
pplReference.Add(envelope.Scan, envelope.LightIntensity);
}
}
if (pplSampleCurve.Count > 0)
{
if (bChecked)
{
panel.AddPoly("", pplSampleCurve, SilacQuantificationConstants.SAMPLE_COLOR, new[] { SilacQuantificationConstants.PLOY_COLOR });
panel.AddPoly("", pplReferenceCurve, SilacQuantificationConstants.REFERENCE_COLOR, new[] { SilacQuantificationConstants.PLOY_COLOR });
}
}
panel.AddCurve("Sample", pplSample, SilacQuantificationConstants.SAMPLE_COLOR, SymbolType.None);
panel.AddCurve("Reference", pplReference, SilacQuantificationConstants.REFERENCE_COLOR, SymbolType.None);
var identified = new PointPairList();
summary.ObservedEnvelopes.FindAll(m => m.IsIdentified).ForEach(m =>
{
identified.Add(new PointPair(m.Scan, m.LightIntensity));
identified.Add(new PointPair(m.Scan, m.HeavyIntensity));
});
panel.AddIndividualLine("Identified", identified, SilacQuantificationConstants.IDENTIFIED_COLOR);
var currentScan = new PointPairList();
summary.ObservedEnvelopes.FindAll(m => m.IsSelected).ForEach(m =>
{
currentScan.Add(new PointPair(m.Scan, Math.Max(m.LightIntensity, m.HeavyIntensity)));
});
if (currentScan.Count > 0)
{
panel.AddIndividualLine("CurrentScan", currentScan, Color.Black);
}
ZedGraphicExtension.UpdateGraph(zgcGraph);
}
public void Update(object sender, UpdateQuantificationItemEventArgs e)
{
var summary = e.Item as O18QuantificationSummaryItem;
if (summary == null)
{
throw new ArgumentNullException("UpdateQuantificationItemEventArgs.Item cannot be null");
}
panel.ClearData();
var pplO16 = new PointPairList();
var pplO18_1 = new PointPairList();
var pplO18_2 = new PointPairList();
var pplO16Curve = new PointPairList();
var pplO18_1Curve = new PointPairList();
var pplO18_2Curve = new PointPairList();
var identified = new PointPairList();
var currentScan = new PointPairList();
bool bChecked = false;
for (int i = 0; i < summary.ObservedEnvelopes.Count; i++)
{
var envelope = summary.ObservedEnvelopes[i];
if (envelope.Enabled != bChecked)
{
if (pplO16Curve.Count > 0)
{
if (bChecked)
{
panel.AddPoly("", pplO16Curve, O18QuantificationConstants.COLOR_O16, new[] { O18QuantificationConstants.COLOR_PLOY });
panel.AddPoly("", pplO18_1Curve, O18QuantificationConstants.COLOR_O18_1, new[] { O18QuantificationConstants.COLOR_PLOY });
panel.AddPoly("", pplO18_2Curve, O18QuantificationConstants.COLOR_O18_2, new[] { O18QuantificationConstants.COLOR_PLOY });
}
}
pplO16Curve = new PointPairList();
pplO18_1Curve = new PointPairList();
pplO18_2Curve = new PointPairList();
bChecked = envelope.Enabled;
}
pplO16.Add(envelope.Scan, envelope[0].Intensity);
pplO18_1.Add(envelope.Scan, envelope[2].Intensity);
pplO18_2.Add(envelope.Scan, envelope[4].Intensity);
pplO16Curve.Add(envelope.Scan, envelope[0].Intensity);
pplO18_1Curve.Add(envelope.Scan, envelope[2].Intensity);
pplO18_2Curve.Add(envelope.Scan, envelope[4].Intensity);
if (envelope.IsIdentified)
{
identified.Add(envelope.Scan, new double[] { envelope[0].Intensity, envelope[2].Intensity, envelope[4].Intensity }.Max());
}
if (envelope.IsSelected)
{
currentScan.Add(envelope.Scan, new double[] { envelope[0].Intensity, envelope[2].Intensity, envelope[4].Intensity }.Max());
}
}
if (pplO16Curve.Count > 0)
{
if (bChecked)
{
panel.AddPoly("", pplO16Curve, O18QuantificationConstants.COLOR_O16, new[] { O18QuantificationConstants.COLOR_PLOY });
panel.AddPoly("", pplO18_1Curve, O18QuantificationConstants.COLOR_O18_1, new[] { O18QuantificationConstants.COLOR_PLOY });
panel.AddPoly("", pplO18_2Curve, O18QuantificationConstants.COLOR_O18_2, new[] { O18QuantificationConstants.COLOR_PLOY });
}
}
panel.AddCurve("O16", pplO16, O18QuantificationConstants.COLOR_O16, SymbolType.None);
panel.AddCurve("O18(1)", pplO18_1, O18QuantificationConstants.COLOR_O18_1, SymbolType.None);
panel.AddCurve("O18(2)", pplO18_2, O18QuantificationConstants.COLOR_O18_2, SymbolType.None);
panel.AddIndividualLine("Identified", identified, O18QuantificationConstants.COLOR_IDENTIFIED);
panel.AddIndividualLine("CurrentScan", currentScan, Color.Black);
ZedGraphicExtension.UpdateGraph(zgcGraph);
}
