private void frm_BasicOne_BasicOneUpdated(object sender, BasicOneChipEventArgs e)
{
//List<mSpotValue> msv = new List<mSpotValue>();
DataTable dt = new DataTable();
string mm; //Records.GetInvalidGlobalHandling(e.RecName);
if (Chips.GetInvalidLocalHandling(e.ChipName) == "No" || Records.GetInvalidGlobalHandling(e.RecName) == "No")
{
mm = "No";
}
else
{
mm = "Yes";
}
dt = SubChips.GetSubChipsmCSM(e.RecName, e.ChipName, Records.GetGlobalMeanMethod(e.RecName), mm);
//msv = SubChips.CalculatemCSM(SubChips.GetSubChipID(e.RecName, e.ChipName, e.Ag),
// Records.GetGlobalMeanMethod(e.RecName),
// mm);
tbp_plot.Controls.Clear();
Chart plot = new Chart();
plot.Dock = DockStyle.Fill;
tbp_plot.Controls.Add(plot);
Series SeriesB = new Series();
Series SeriesR = new Series();
plot.Series.Add(SeriesB);
plot.Series.Add(SeriesR);
//SeriesB.ToolTip = "Value: #VALY\r\nSample: #LABEL";
//SeriesR.ToolTip = "Value: #VALY\r\nSample: #LABEL";
ChartArea blue = new ChartArea("ChartBlue");
ChartArea red = new ChartArea("ChartRed");
blue.AxisX.Title = "samples";
red.AxisX.Title = "samples";
plot.ChartAreas.Add(blue);
plot.ChartAreas.Add(red);
SeriesB.ChartArea = "ChartBlue";
SeriesR.ChartArea = "ChartRed";
SeriesB.ChartType = SeriesChartType.Column;
SeriesR.ChartType = SeriesChartType.Column;
int i;
int agid, agnormid;
rv.Clear();
rn.Clear();
bv.Clear();
bn.Clear();
agid = Records.GetAntigeneID(e.RecName, e.Ag);
csv_ag_n = e.Ag;
csv_agnorm_n = e.AgNorm;
foreach (DataRow dr in dt.Rows)
{
mSpotValue msvag = (mSpotValue)dr[agid];
double agnormb, agnormr;
if (e.AgNorm == "---")
{
string tooltip;
DataPoint b = new DataPoint();
DataPoint r = new DataPoint();
b.SetValueY(msvag.bvalue * 0xFFFF);
b.AxisLabel = msvag.subchipID;
//CSV
bv.Add(msvag.bvalue * 0xFFFF);
bn.Add(msvag.samplename);
tooltip = "Value: #VALY\r\nSample: " + msvag.samplename + "\r\nSpots used: ";
if (msvag.bvalid == false)
{
b.Color = Color.LightBlue;
}
else
{
b.Color = Color.Blue;
}
tooltip += msvag.bspotsused.ToString();
b.ToolTip = tooltip;
blue.AxisY.Title = e.Ag;
blue.AxisX.Interval = 1;
r.SetValueY(msvag.rvalue * 0xFFFF);
r.AxisLabel = msvag.subchipID;
//CSV
rv.Add(msvag.rvalue * 0xFFFF);
rn.Add(msvag.samplename);
tooltip = "Value: #VALY\r\nSample: " + msvag.samplename + "\r\nSpots used: ";
if (msvag.bvalid == false)
{
r.Color = Color.LightCoral;
}
else
{
r.Color = Color.Red;
}
tooltip += msvag.bspotsused.ToString();
r.ToolTip = tooltip;
red.AxisY.Title = e.Ag;
red.AxisX.Interval = 1;
SeriesB.Points.Add(b);
SeriesR.Points.Add(r);
}
else
{
agnormid = Records.GetAntigeneID(e.RecName, e.AgNorm);
mSpotValue msvagnorm = (mSpotValue)dr[agnormid];
agnormb = msvagnorm.bvalue;
agnormr = msvagnorm.rvalue;
SeriesB.Points.Add(msvag.bvalue / agnormb);
blue.AxisY.Title = e.Ag + " / " + e.AgNorm;
SeriesR.Points.Add(msvag.rvalue / agnormr);
red.AxisY.Title = e.Ag + " / " + e.AgNorm;
//CSV
bv.Add(msvag.bvalue * 0xFFFF);
bn.Add(msvag.samplename);
rv.Add(msvag.bvalue * 0xFFFF);
rn.Add(msvag.samplename);
}
}
}
public List <mSpotValue> CalculatemCSM(DataRow dr, string mm, string invh)
{
List <mSpotValue> retlist = new List <mSpotValue>();
int i, j, k;
double m1, m2, m3;
//DataRow dr;
//dr = Data.Rows[ID];
if (mm == "UK")
{
k = 13;
}
else
{
k = 1;
}
j = 0;
for (i = 0; i < 52; ++i)
{
mSpotValue msv = new mSpotValue();
//Blue channel
m1 = Convert.ToDouble(dr[dr.Table.Columns["CSMb0"].Ordinal + j + 0 * k]);
m2 = Convert.ToDouble(dr[dr.Table.Columns["CSMb0"].Ordinal + j + 1 * k]);
m3 = Convert.ToDouble(dr[dr.Table.Columns["CSMb0"].Ordinal + j + 2 * k]);
msv.bvalid = true;
msv.bspotsused = 3;
double mean = 0;
int divisor = 0;
if (m1 > 2)
{
m1 = m1 - 10f;
msv.bspotsused--;
msv.bvalid = false;
}
else
{
mean += m1;
divisor++;
}
if (m2 > 2)
{
m2 = m2 - 10f;
msv.bspotsused--;
msv.bvalid = false;
}
else
{
mean += m2;
divisor++;
}
if (m3 > 2)
{
m3 = m3 - 10f;
msv.bspotsused--;
msv.bvalid = false;
}
else
{
mean += m3;
divisor++;
}
//if (invh == "Yes") msv.bvalid = true;
msv.bvalue = (m1 + m2 + m3) / 3;
//Red channel
m1 = Convert.ToDouble(dr[dr.Table.Columns["CSMr0"].Ordinal + j + 0 * k]);
m2 = Convert.ToDouble(dr[dr.Table.Columns["CSMr0"].Ordinal + j + 1 * k]);
m3 = Convert.ToDouble(dr[dr.Table.Columns["CSMr0"].Ordinal + j + 2 * k]);
msv.rvalid = true;
if (m1 > 2)
{
m1 = m1 - 10f;
msv.rvalid = false;
}
if (m2 > 2)
{
m2 = m2 - 10f;
msv.rvalid = false;
}
if (m3 > 2)
{
m3 = m3 - 10f;
msv.rvalid = false;
}
//if (invh == "Yes") msv.rvalid = true;
msv.rvalue = (m1 + m2 + m3) / 3;
//add samplename and subchipID
msv.samplename = dr["samplename"].ToString();
msv.subchipID = dr["subchipID"].ToString();
//add to list
retlist.Add(msv);
//next loop
if (mm == "UK")
{
j += 1;
}
else
{
j += 3;
}
}
return(retlist);
}
