private void sigmoidFittToolStripMenuItem_Click(object sender, EventArgs e)
{
int NumberOfReplicate = 4;
int NumberOfCurves = 30;
int DRCNumberofPoints = 12;
cExtendedTable FullResults = new cExtendedTable();
FullResults.ListRowNames = new List<string>();
double RealEC50 = 0.0001;
int NumberOfDiffEC50 = 20;
int NumberOfSlopes = 100;
// for (int EC50 = 0; EC50 < NumberOfDiffEC50; EC50++)
{
//double TmpSlopeValue = 0.0;
for (int EC50 = 0; EC50 < NumberOfDiffEC50; EC50++)
//for (int SlopeTmp = 0; SlopeTmp < NumberOfSlopes; SlopeTmp++)
{
RealEC50 *= 2;
//TmpSlopeValue += 0.1;
cExtendedList ListPValues = new cExtendedList("EC50 - " + RealEC50/*TmpSlopeValue*/);
cDesignerTab DT = new cDesignerTab();
DT.IsMultiline = false;
cDataGeneratorTitration GT = new cDataGeneratorTitration();
GT.NumberOfPoint = DRCNumberofPoints;
GT.Start = 5;
GT.DilutionFactor = 2;
GT.Run();
Random RND = new Random();
//cExtendedTable EXT = new cExtendedTable();
//EXT.ListRowNames = new List<string>();
//EXT.Name = "Quality fitting Report";
//EXT.Add(new cExtendedList("Noise Level"));
//EXT.Add(new cExtendedList("Original EC50"));
//EXT.Add(new cExtendedList("Estimated EC50"));
//EXT.Add(new cExtendedList("p-Value"));
for (int i = 0; i < NumberOfCurves; i++)
{
cExtendedTable FinalTable = new cExtendedTable();
cDataGeneratorSigmoid DGS = null;
DGS = new cDataGeneratorSigmoid();
DGS.SetInputData(GT.GetOutPut()[0]);
DGS.EC50 = RealEC50;// 0.01;// 1;// 0.1;// Math.Pow((double)10, -2);
DGS.Bottom = 0;
DGS.Top = 100;
DGS.Slope = 2;// TmpSlopeValue;//1;
DGS.Run();
cAddNoise AN = new cAddNoise();
AN.DistributionType = eRandDistributionType.GAUSSIAN;
double NoiseLevel = (i + 1) * 5;
// EXT[0].Add(NoiseLevel);
// EXT[1].Add(DGS.EC50);
#region loop over the replicates
for (int NumReplic = 0; NumReplic < NumberOfReplicate; NumReplic++)
{
//AN.Mean = 0;
//AN.Stdv = 10;
//AN.SetInputData(DGS.GetOutPut());
//AN.Run();
cExtendedList NewL = DGS.GetOutPut()[1];
for (int k = 0; k < NewL.Count; k++)
{
NewL[k] += RND.NextDouble() * NoiseLevel;
}
cExtendedTable TmpTable = new cExtendedTable();
TmpTable.Add(GT.GetOutPut()[0]);
TmpTable.Add(/*AN.GetOutPut()[1]*/NewL);
if (NumReplic >= 1)
{
cMerge M = new cMerge();
M.IsHorizontal = false;
M.SetInputData(TmpTable, FinalTable);
M.Run();
FinalTable = M.GetOutPut();
}
else
FinalTable = new cExtendedTable(TmpTable);
}
#endregion
//cListWell LW = new cListWell(null);
//foreach (cWell item in CompleteScreening.GetCurrentDisplayPlate().ListActiveWells)
//{
// if (item.GetClassIdx() == 0)
// LW.Add(item);
//}
//cExtendedTable ET = LW.GetDescriptorValues(CompleteScreening.ListDescriptors.GetActiveDescriptors(), true);
cCurveForGraph CFG = new cCurveForGraph();
CFG.SetInputData(FinalTable);
CFG.Run();
// compute ANOVA
cANOVA S = new cANOVA();
cExtendedTable NewTable = CFG.ListPtValues.Crop(0, CFG.ListPtValues.Count - 1, 1, CFG.ListPtValues[0].Count - 1);
S.SignificanceThreshold = 1E-11;
S.SetInputData(NewTable);
S.Run();
//cLinearRegression LR = new cLinearRegression();
//LR.SetInputData(FinalTable);
//LR.Run();
cSigmoidFitting SF = new cSigmoidFitting();
SF.SetInputData(FinalTable);
if (SF.Run().IsSucceed == false) continue;
// double Ratio = LR.GetOutPut()[0][LR.GetOutPut().Count - 1] / SF.GetOutPut()[0][SF.GetOutPut().Count - 1];
cExtendedTable Sigmoid = SF.GetFittedRawValues(GT.GetOutPut()[0]);
FinalTable[0] = Sigmoid[1];
cDesignerSplitter DS = new cDesignerSplitter();
////cViewerTableAsRichText VT = new cViewerTableAsRichText();
cViewerTable VT = new cViewerTable();
cExtendedTable TableResults = SF.GetOutPut();
TableResults[0].Add(S.GetOutPut()[0][0]);
ListPValues.Add(S.GetOutPut()[0][0]);
TableResults[0].Add(S.GetOutPut()[0][1]);
TableResults.ListRowNames.Add("p-Value");
TableResults.ListRowNames.Add("Null hyp. rejected?");
VT.SetInputData(TableResults);
VT.DigitNumber = -1;
VT.Run();
cViewerGraph1D VS1 = new cViewerGraph1D();
VS1.SetInputData(new cExtendedTable(Sigmoid[1]));
VS1.AddCurve(CFG);
VS1.Chart.X_AxisValues = Sigmoid[0];//DGS.GetOutPut()[0];
VS1.Chart.IsLogAxis = true;
VS1.Chart.IsLine = true;
VS1.Chart.IsShadow = true;
VS1.Chart.Opacity = 210;
VS1.Chart.LineWidth = 3;
VS1.Chart.LabelAxisX = "Concentration";
VS1.Chart.LabelAxisY = "Readout";
VS1.Chart.XAxisFormatDigitNumber = -1;
VS1.Chart.IsZoomableX = true;
//Classes.Base_Classes.General.cLineVerticalForGraph VLForEC50 = new Classes.Base_Classes.General.cLineVerticalForGraph(SF.GetOutPut()[0][2]);
//VLForEC50.AddText("EC50: " + SF.GetOutPut()[0][2].ToString("e3")/* + "\nError Ratio:" + Ratio.ToString("N4")*/);
//VS1.Chart.ListVerticalLines.Add(VLForEC50);
//VS1.Chart.ArraySeriesInfo = new cSerieInfoDesign[FinalTable.Count];
//EXT[2].Add(SF.GetOutPut()[0][2]);
//EXT[3].Add(S.GetOutPut()[0][0]);
//for (int IdxCurve = 0; IdxCurve < FinalTable.Count; IdxCurve++)
//{
// cSerieInfoDesign TmpSerieInfo = new cSerieInfoDesign();
// TmpSerieInfo.color = Color.FromArgb(100, GlobalInfo.ListCellularPhenotypes[IdxCurve % GlobalInfo.ListCellularPhenotypes.Count].ColourForDisplay);
// TmpSerieInfo.markerStyle = MarkerStyle.Circle;
// VS1.Chart.ArraySeriesInfo[IdxCurve] = TmpSerieInfo;
//}
VS1.Run();
DS.SetInputData(VS1.GetOutPut());
DS.SetInputData(VT.GetOutPut());
DS.Orientation = Orientation.Horizontal;
DS.Title = "Noise Stdev " + NoiseLevel;
DS.Run();
DT.SetInputData(DS.GetOutPut());
// EXT.ListRowNames.Add(DS.Title);
}
DT.Run();
//cDisplayToWindow DTW = new cDisplayToWindow();
//DTW.SetInputData(DT.GetOutPut());
//DTW.Run();
//DTW.Display();
//cDisplayExtendedTable DET = new cDisplayExtendedTable();
//DET.SetInputData(EXT);
//DET.DigitNumber = -1;
//DET.Run();
FullResults.Add(ListPValues);
}
}
cDisplayExtendedTable DET = new cDisplayExtendedTable();
DET.SetInputData(FullResults);
DET.DigitNumber = -1;
DET.Run();
}
private void aNOVAToolStripMenuItem1_Click(object sender, EventArgs e)
{
cGUI_ListClasses GUI_ListClasses = new cGUI_ListClasses();
GUI_ListClasses.IsCheckBoxes = true;
GUI_ListClasses.IsSelectAll = true;
if (GUI_ListClasses.Run().IsSucceed == false) return;
cExtendedTable ListClassSelected = GUI_ListClasses.GetOutPut();
string SubTitle = "ANOVA (One-Way)";
#region single plate and plate by plate
cDesignerTab DT = new cDesignerTab();
if ((ProcessModeCurrentPlateOnlyToolStripMenuItem.Checked) || (ProcessModeplateByPlateToolStripMenuItem.Checked)/*||(ProcessModeEntireScreeningToolStripMenuItem.Checked)*/)
{
List<cPlate> ListPlatesToProcess = new List<cPlate>();
if ((ProcessModeplateByPlateToolStripMenuItem.Checked)/*||(ProcessModeEntireScreeningToolStripMenuItem.Checked)*/)
{
foreach (cPlate TmpPlate in cGlobalInfo.CurrentScreening.ListPlatesActive)
ListPlatesToProcess.Add(TmpPlate);
}
else
ListPlatesToProcess.Add(cGlobalInfo.CurrentScreening.GetCurrentDisplayPlate());
foreach (cPlate TmpPlate in ListPlatesToProcess)
{
cExtendedTable NewTable1 = new cExtendedTable();
List<cListWells> ListListWells = new List<cListWells>();
int NumWells = 0;
for (int IdxClassWell = 0; IdxClassWell < ListClassSelected[0].Count; IdxClassWell++)
{
if (ListClassSelected[0][IdxClassWell] >= 1)
{
List<cWellClassType> LCT = new List<cWellClassType>();
LCT.Add(cGlobalInfo.ListWellClasses[IdxClassWell]);
cListWells TmpList = TmpPlate.ListWells.Filter(LCT);
if (TmpList.Count >= 3)
{
ListListWells.Add(TmpList);
NumWells += TmpList.Count;
}
}
}
if (ListListWells.Count <= 1)
{
if (ProcessModeCurrentPlateOnlyToolStripMenuItem.Checked)
{
MessageBox.Show("Insufficient number of control wells", "Error", MessageBoxButtons.OK, MessageBoxIcon.Error);
return;
}
else
continue;
}
cExtendedTable CompleteReport = null;
cExtendedList ListZ = new cExtendedList();
List<cDescriptorType> ListDescForZFactor = new List<cDescriptorType>();
List<string> ListNames = new List<string>();
int RealIdx = 0;
for (int IDxDesc = 0; IDxDesc < cGlobalInfo.CurrentScreening.ListDescriptors.Count; IDxDesc++)
{
if (!cGlobalInfo.CurrentScreening.ListDescriptors[IDxDesc].IsActive()) continue;
cExtendedTable TableForZ = new cExtendedTable();
foreach (var item in ListListWells)
{
List<cDescriptorType> LType = new List<cDescriptorType>();
LType.Add(cGlobalInfo.CurrentScreening.ListDescriptors[IDxDesc]);
cExtendedTable TTable = item.GetAverageDescriptorValues(LType, false, false);
TableForZ.Add(TTable[0]);
}
RealIdx++;
cANOVA ZF = new cANOVA();
ZF.SetInputData(TableForZ);
ZF.Run();
ListZ.Add(ZF.GetOutPut()[0][0]);
ZF.GetOutPut()[0].Name = cGlobalInfo.CurrentScreening.ListDescriptors[IDxDesc].GetName();
if (CompleteReport == null)
CompleteReport = new cExtendedTable(ZF.GetOutPut());
else
{
cMerge M = new cMerge();
M.IsHorizontal = true;
M.SetInputData(CompleteReport, ZF.GetOutPut());
M.Run();
CompleteReport = M.GetOutPut();
}
ListDescForZFactor.Add(cGlobalInfo.CurrentScreening.ListDescriptors[IDxDesc]);
}
cExtendedTable ET = new cExtendedTable(new cExtendedTable(ListZ));
ET[0].ListTags = new List<object>();
ET[0].ListTags.AddRange(ListDescForZFactor);
ET.Name = TmpPlate.GetName() + "\n" + SubTitle + " - " + NumWells + " wells";// +" - " +cGlobalInfo.ListWellClasses[IdxClassNeg].Name + " (" + NewTable1[0].Count + " wells) vs. " +cGlobalInfo.ListWellClasses[IdxClassPos].Name + " (" + NewTable2[0].Count + " wells)";
ET[0].Name = ET.Name;
cSort S = new cSort();
S.SetInputData(ET);
S.IsAscending = false;
S.ColumnIndexForSorting = 0;
S.Run();
//ZFactorList.Sort(delegate(cSimpleSignature p1, cSimpleSignature p2) { return p1.AverageValue.CompareTo(p2.AverageValue); });
cViewerGraph1D VG1 = new cViewerGraph1D();
VG1.SetInputData(S.GetOutPut());
VG1.Chart.LabelAxisY = SubTitle;
VG1.Chart.LabelAxisX = "Descriptor";
VG1.Chart.IsZoomableX = true;
VG1.Chart.IsBar = true;
VG1.Chart.IsBorder = true;
VG1.Chart.IsDisplayValues = true;
VG1.Chart.IsShadow = true;
VG1.Chart.MarkerSize = 4;
//VG1.Chart.Max
VG1.Title = TmpPlate.GetName();
// Classes.Base_Classes.General.cLineHorizontalForGraph VLZ05 = new Classes.Base_Classes.General.cLineHorizontalForGraph(.05);
// VLZ05.IsAllowMoving = true;
// VG1.Chart.ListHorizontalLines.Add(VLZ05);
VG1.Run();
cDesignerSplitter DS = new cDesignerSplitter();
DS.Orientation = Orientation.Vertical;
DS.SetInputData(VG1.GetOutPut());
cTranspose T = new cTranspose();
T.SetInputData(CompleteReport);
T.Run();
CompleteReport = T.GetOutPut();
CompleteReport[0].Name = "P-Value";
CompleteReport[1].Name = "Is significant ?";
cSort S1 = new cSort();
S1.SetInputData(CompleteReport);
S1.ColumnIndexForSorting = 0;
S1.IsAscending = true;
S1.Run();
cViewerTable VT = new cViewerTable();
VT.SetInputData(S1.GetOutPut());
VT.DigitNumber = -1;
VT.Run();
DS.SetInputData(VT.GetOutPut());
DS.Title = TmpPlate.GetName();
DS.Run();
DT.SetInputData(DS.GetOutPut());
}
DT.Run();
cDisplayToWindow CDW = new cDisplayToWindow();
CDW.SetInputData(DT.GetOutPut());//VG1.GetOutPut());
if (ProcessModeCurrentPlateOnlyToolStripMenuItem.Checked)
CDW.Title = SubTitle + " - " + ListPlatesToProcess[0].GetName();
else
CDW.Title = SubTitle + " - " + ListPlatesToProcess.Count + " plates";
CDW.Run();
CDW.Display();
}
#endregion
#region entire screening
else if (ProcessModeEntireScreeningToolStripMenuItem.Checked)
{
List<cPlate> ListPlatesToProcess = new List<cPlate>();
foreach (cPlate TmpPlate in cGlobalInfo.CurrentScreening.ListPlatesActive)
ListPlatesToProcess.Add(TmpPlate);
cExtendedList ListZ = new cExtendedList();
List<cPlate> ListPlatesForZFactor = new List<cPlate>();
foreach (cPlate TmpPlate in ListPlatesToProcess)
{
cListWells ListWellsToProcess1 = new cListWells(null);
cListWells ListWellsToProcess2 = new cListWells(null);
foreach (cWell item in TmpPlate.ListActiveWells)
{
if (item.GetCurrentClassIdx() != -1)
{
if (ListClassSelected[0][item.GetCurrentClassIdx()] == 1)
ListWellsToProcess1.Add(item);
if (ListClassSelected[1][item.GetCurrentClassIdx()] == 1)
ListWellsToProcess2.Add(item);
}
}
cExtendedTable NewTable1 = new cExtendedTable(ListWellsToProcess1, cGlobalInfo.CurrentScreening.ListDescriptors.GetDescriptorIndex(cGlobalInfo.CurrentScreening.ListDescriptors.GetActiveDescriptor()));
cExtendedTable NewTable2 = new cExtendedTable(ListWellsToProcess2, cGlobalInfo.CurrentScreening.ListDescriptors.GetDescriptorIndex(cGlobalInfo.CurrentScreening.ListDescriptors.GetActiveDescriptor()));
//cExtendedTable NewTable2 = new cExtendedTable(ListWellsToProcess2, false);
if ((NewTable1.Count == 0) || (NewTable1[0].Count < 2) || (NewTable2.Count == 0) || (NewTable2[0].Count < 2))
{
if (ProcessModeCurrentPlateOnlyToolStripMenuItem.Checked)
{
MessageBox.Show("Insufficient number of control wells", "Error", MessageBoxButtons.OK, MessageBoxIcon.Error);
return;
}
else
continue;
}
cExtendedTable TableForZ = new cExtendedTable();
TableForZ.Add(NewTable1[0]);
TableForZ.Add(NewTable2[0]);
cStudent_tTest ZF = new cStudent_tTest();
ZF.SetInputData(TableForZ);
ZF.Run();
double Zfactor = ZF.GetOutPut()[0][1];
ListZ.Add(Zfactor);
// update plate quality
//TmpPlate.ListProperties.UpdateValueByName("Quality", Math.Exp(Zfactor - 1));
//cProperty Prop = TmpPlate.ListProperties.FindByName("Quality");
//Prop.Info = ZF.GetInfo();
ListPlatesForZFactor.Add(TmpPlate);
}
cExtendedTable ET = new cExtendedTable(new cExtendedTable(ListZ));
ET[0].ListTags = new List<object>();
ET[0].ListTags.AddRange(ListPlatesForZFactor);
ET.Name = SubTitle + " - " + cGlobalInfo.CurrentScreening.ListDescriptors[cGlobalInfo.CurrentScreening.ListDescriptors.CurrentSelectedDescriptorIdx].GetName();// +" - " +cGlobalInfo.ListWellClasses[IdxClassNeg].Name + /*" (" + NewTable1[0].Count + " wells)*/ " vs. " +cGlobalInfo.ListWellClasses[IdxClassPos].Name;// +" (" + NewTable2[0].Count + " wells)";
ET[0].Name = ET.Name;
cViewerGraph1D VG1 = new cViewerGraph1D();
VG1.SetInputData(ET);
VG1.Chart.LabelAxisY = SubTitle;
VG1.Chart.LabelAxisX = "Plate";
VG1.Chart.IsZoomableX = true;
VG1.Chart.IsBar = true;
VG1.Chart.IsBorder = true;
VG1.Chart.IsDisplayValues = true;
VG1.Chart.IsShadow = true;
VG1.Chart.MarkerSize = 4;
VG1.Title = cGlobalInfo.CurrentScreening.ListDescriptors[cGlobalInfo.CurrentScreening.ListDescriptors.CurrentSelectedDescriptorIdx].GetName();
VG1.Run();
cDisplayToWindow CDW = new cDisplayToWindow();
CDW.SetInputData(VG1.GetOutPut());
CDW.Title = SubTitle + " - " + ListPlatesToProcess.Count + " plates";
CDW.Run();
CDW.Display();
#endregion
}
}
private void PerformTestType(eTestType TestType)
{
#region extract classes of interest
cGUI_ListClasses GUI_ListClasses = new cGUI_ListClasses();
GUI_ListClasses.IsCheckBoxes = !_DescEvolCellByCellItem.Checked;
GUI_ListClasses.IsSelectAll = true;
if (GUI_ListClasses.Run().IsSucceed == false) return;
cExtendedTable ListClassSelected = GUI_ListClasses.GetOutPut();
if (ListClassSelected.Sum() < 1)
{
MessageBox.Show("At least one classe has to be selected.", "Error !", MessageBoxButtons.OK, MessageBoxIcon.Error);
return;
}
#endregion
cDesignerTab DT = new cDesignerTab();
for (int i = 0; i < cGlobalInfo.CurrentScreening.ListDescriptors.Count; i++)
{
if (cGlobalInfo.CurrentScreening.ListDescriptors[i].IsActive() == false) continue;
cDesignerSplitter DS = new cDesignerSplitter();
DS.Orientation = Orientation.Vertical;
cViewerTable VTable = new cViewerTable();
cViewertext VT = new cViewertext();
cExtendedTable FinalTable = new cExtendedTable(cGlobalInfo.ListWellClasses.Count, 0, 0);
for (int j = 0; j < cGlobalInfo.ListWellClasses.Count; j++)
{
FinalTable[j].Tag = cGlobalInfo.ListWellClasses[j];
FinalTable[j].Name = cGlobalInfo.ListWellClasses[j].Name;
}
cListWells ListWellsToProcess = new cListWells(null);
cDescriptorType CurrentDesc = cGlobalInfo.CurrentScreening.ListDescriptors[i];
// build the table
foreach (cPlate CurrentPlate in cGlobalInfo.CurrentScreening.ListPlatesActive)
{
foreach (cWell CurrentWell in CurrentPlate.ListActiveWells)
{
if ((CurrentWell.GetCurrentClassIdx() < 0) || (ListClassSelected[0][CurrentWell.GetCurrentClassIdx()] == 0)) continue;
{
FinalTable[CurrentWell.GetCurrentClassIdx()].Add(CurrentWell.GetAverageValue(CurrentDesc));
ListWellsToProcess.Add(CurrentWell);
}
}
}
cClean C = new cClean();
C.SetInputData(FinalTable);
C.Run();
cExtendedTable NewTable = C.GetOutPut();
cTwoSampleFTest TSFTest = null;
cANOVA Anova = null;
cTwoSampleUnpooledTTest TwoSampleTTest = null;
cStudent_tTest StudentTTest = null;
if (TestType == eTestType.F_TEST)
{
TSFTest = new cTwoSampleFTest();
TSFTest.SetInputData(NewTable);
TSFTest.Run();
VTable.SetInputData(TSFTest.GetOutPut());
VT.SetInputData(TSFTest.GetInfo());
}
else if (TestType == eTestType.ANOVA)
{
Anova = new cANOVA();
Anova.SetInputData(NewTable);
Anova.Run();
VTable.SetInputData(Anova.GetOutPut());
VT.SetInputData(Anova.GetInfo());
}
else if (TestType == eTestType.TWO_SAMPLES_T_TEST)
{
TwoSampleTTest = new cTwoSampleUnpooledTTest();
TwoSampleTTest.SetInputData(NewTable);
TwoSampleTTest.Run();
VTable.SetInputData(TwoSampleTTest.GetOutPut());
VT.SetInputData(TwoSampleTTest.GetInfo());
}
else if (TestType == eTestType.STUDENT_T_TEST)
{
StudentTTest = new cStudent_tTest();
StudentTTest.SetInputData(NewTable);
StudentTTest.Run();
VTable.SetInputData(StudentTTest.GetOutPut());
VT.SetInputData(StudentTTest.GetInfo());
}
VTable.DigitNumber = -1;
VTable.Run();
DS.SetInputData(VTable.GetOutPut());
VT.Run();
DS.SetInputData(VT.GetOutPut());
DS.Run();
DS.Title = cGlobalInfo.CurrentScreening.ListDescriptors[i].GetName();
cDesignerSplitter DSMain = new cDesignerSplitter();
DSMain.Orientation = Orientation.Horizontal;
DSMain.SetInputData(DS.GetOutPut());
// Compute and display associated Stacked histogram
//CDW1.Title = CompleteScreening.ListDescriptors[CompleteScreening.ListDescriptors.CurrentSelectedDescriptorIdx].GetName() + " - Stacked Histogram - " + PlateList.Count + " plates";
cExtendedTable TableForHisto = ListWellsToProcess.GetAverageDescriptorValues(i);
TableForHisto.Name = "";
cViewerStackedHistogram CV1 = new cViewerStackedHistogram();
CV1.SetInputData(TableForHisto);
CV1.Chart.LabelAxisX = cGlobalInfo.CurrentScreening.ListDescriptors[i].GetName();
CV1.Chart.IsLegend = true;
CV1.Run();
DSMain.SetInputData(CV1.GetOutPut());
DSMain.Title = cGlobalInfo.CurrentScreening.ListDescriptors[i].GetName();
DSMain.Run();
DT.SetInputData(DSMain.GetOutPut());
}
DT.Run();
cDisplayToWindow DTW = new cDisplayToWindow();
DTW.SetInputData(DT.GetOutPut());
DTW.Title = "Classification Significance - " + TestType.ToString();
DTW.Run();
DTW.Display();
}
void ToolStripMenuItem_DRCAnalysis_Click(object sender, EventArgs e)
{
List<cDescriptorType> ListSelectedDesc = new List<cDescriptorType>();// GlobalInfo.CurrentScreening.ListDescriptors.GetActiveDescriptors();
ListSelectedDesc.Add(cGlobalInfo.CurrentScreening.ListDescriptors.GetActiveDescriptor());
//if (GlobalInfo == null) GlobalInfo = cGlobalInfo.CurrentScreening.GlobalInfo;
//if (cGlobalInfo == null) return;
cDesignerMultiChoices DT = new cDesignerMultiChoices();
cExtendedTable TableForGeneralResuts = new cExtendedTable();
TableForGeneralResuts.Add(new cExtendedList("p-Value"));
TableForGeneralResuts.Add(new cExtendedList("EC50"));
TableForGeneralResuts.Add(new cExtendedList("Slope"));
TableForGeneralResuts.Add(new cExtendedList("Bottom"));
TableForGeneralResuts.Add(new cExtendedList("Top"));
TableForGeneralResuts.Add(new cExtendedList("Window"));
TableForGeneralResuts.Add(new cExtendedList("Area Under Curve"));
TableForGeneralResuts.ListRowNames = new List<string>();
TableForGeneralResuts.ListTags = new List<object>();
cDesignerSplitter MainSplitter = new cDesignerSplitter();
int IdxNode = 0;
cListListWells CurrentSelectedGroups = this.BuildListListWells();
foreach (cListWells TmpListWells in CurrentSelectedGroups)
{
// List<cDescriptorType> LType = new List<cDescriptorType>();
// LType.Add(ListSelectedDesc[i]);
cExtendedTable CompleteTable = TmpListWells.GetAverageDescriptorValues(ListSelectedDesc, true, false);
//cExtendedTable CompleteTable = GLP.GetOutPut();
cCurveForGraph CFG = new cCurveForGraph();
CFG.SetInputData(CompleteTable);
CFG.Run();
cSigmoidFitting SF = new cSigmoidFitting();
SF.SetInputData(CompleteTable);
if (SF.Run().IsSucceed == false) continue;
// double Ratio = LR.GetOutPut()[0][LR.GetOutPut().Count - 1] / SF.GetOutPut()[0][SF.GetOutPut().Count - 1];
cANOVA A = new cANOVA();
cExtendedTable NewTable = CFG.ListPtValues.Crop(0, CFG.ListPtValues.Count - 1, 1, CFG.ListPtValues[0].Count - 1);
A.SignificanceThreshold = 1E-11;
A.SetInputData(NewTable);
A.Run();
cExtendedTable Sigmoid = SF.GetFittedRawValues(CFG.GetListXValues());
CompleteTable[0] = Sigmoid[1];
CompleteTable[0].Name = ListSelectedDesc[0].GetName() + "\n" + Sigmoid[1].Name;
cDesignerSplitter DS = new cDesignerSplitter();
//cViewerTableAsRichText VT = new cViewerTableAsRichText();
cViewerTable VT = new cViewerTable();
cExtendedTable TableResults = SF.GetOutPut();
if ((A.GetOutPut() != null) && (A.GetOutPut().Count > 0))
{
TableResults[0].Add(A.GetOutPut()[0][0]);
TableResults[0].Add(A.GetOutPut()[0][1]);
TableResults.ListRowNames.Add("p-Value");
TableResults.ListRowNames.Add("Rejected?");
TableForGeneralResuts[0].Add(A.GetOutPut()[0][0]);
}
else
{
TableForGeneralResuts[0].Add(1);
}
TableResults.Name = TV.Nodes[IdxNode].Text;
TableResults[0].Name = "Fitting Parameters";
VT.SetInputData(TableResults);
VT.DigitNumber = -1;
VT.Run();
cViewerGraph1D VS1 = new cViewerGraph1D();
cExtendedTable MyTable = new cExtendedTable(Sigmoid[1]);
MyTable.Name = TmpListWells.Name;// TV.Nodes[IdxNode].Text;
VS1.SetInputData(MyTable);
VS1.AddCurve(CFG);
VS1.Chart.X_AxisValues = Sigmoid[0];
VS1.Chart.IsLogAxis = true;
VS1.Chart.IsLine = true;
VS1.Chart.IsShadow = false;
VS1.Chart.Opacity = 210;
VS1.Chart.LineWidth = 3;
VS1.Chart.MarkerSize = 8;
VS1.Chart.IsDisplayValues = cGlobalInfo.OptionsWindow.FFAllOptions.checkBoxDRCDisplayValues.Checked;
VS1.Chart.LabelAxisX = "Concentration";
VS1.Chart.LabelAxisY = CompleteTable[1].Name;
VS1.Chart.XAxisFormatDigitNumber = cGlobalInfo.OptionsWindow.FFAllOptions.GetDRCNumberOfDigit();
VS1.Chart.IsZoomableX = true;
Classes.Base_Classes.General.cLineVerticalForGraph VLForEC50 = new Classes.Base_Classes.General.cLineVerticalForGraph(SF.GetOutPut()[0][2]);
//VLForEC50.AddText("EC50: " + SF.GetOutPut()[0][2].ToString("e3")/* + "\nError Ratio:" + Ratio.ToString("N4")*/);
VS1.Chart.ListVerticalLines.Add(VLForEC50);
TableForGeneralResuts.ListRowNames.Add(TV.Nodes[IdxNode].Text);
TableForGeneralResuts.ListTags.Add((Chart)VS1.Chart);//ListSelectedDesc[0]);
//EC50
TableForGeneralResuts[1].Add(SF.GetOutPut()[0][2]);
// Slope
TableForGeneralResuts[2].Add(SF.GetOutPut()[0][3]);
// Bottom
TableForGeneralResuts[3].Add(SF.GetOutPut()[0][0]);
// Top
TableForGeneralResuts[4].Add(SF.GetOutPut()[0][1]);
// Window
double Window = SF.GetOutPut()[0][1] / SF.GetOutPut()[0][0];
TableForGeneralResuts[5].Add(Window);
TableForGeneralResuts[6].Add(SF.GetOutPut()[0][5]);
VS1.Chart.ArraySeriesInfo = new cSerieInfoDesign[CompleteTable.Count];
for (int IdxCurve = 0; IdxCurve < CompleteTable.Count; IdxCurve++)
{
cSerieInfoDesign TmpSerieInfo = new cSerieInfoDesign();
TmpSerieInfo.color = cGlobalInfo.ListCellularPhenotypes[IdxCurve % cGlobalInfo.ListCellularPhenotypes.Count].ColourForDisplay;
TmpSerieInfo.markerStyle = MarkerStyle.None;
VS1.Chart.ArraySeriesInfo[IdxCurve] = TmpSerieInfo;
}
VS1.Run();
DS.SetInputData(VS1.GetOutPut());
DS.SetInputData(VT.GetOutPut());
DS.Orientation = Orientation.Horizontal;
DS.Title = TV.Nodes[IdxNode++].Text; //TmpListWells.ti;// ListSelectedDesc[0].GetName();
DS.Run();
DT.SetInputData(DS.GetOutPut());
}
DT.Run();
cExtendedControl TextEC = DT.GetOutPut();
if (TextEC == null) return;
TextEC.Width = 0;
TextEC.Height = 0;
TextEC.Anchor = (System.Windows.Forms.AnchorStyles)(System.Windows.Forms.AnchorStyles.Top | System.Windows.Forms.AnchorStyles.Bottom
| System.Windows.Forms.AnchorStyles.Left | System.Windows.Forms.AnchorStyles.Right);
MainSplitter.SetInputData(TextEC);
string DRCsortingMethod = cGlobalInfo.OptionsWindow.FFAllOptions.comboBoxDRCSorting.SelectedItem.ToString();
//comboBoxDRCSorting
int IdxColSorting = 0;
cExtendedTable RT = TableForGeneralResuts;
switch (DRCsortingMethod)
{
case "ANOVA":
IdxColSorting = 0;
break;
case "EC50":
IdxColSorting = 1;
break;
case "Window":
IdxColSorting = 5;
break;
case "AUC":
IdxColSorting = 6;
break;
case "None":
IdxColSorting = -1;
break;
default:
break;
}
if (IdxColSorting != -1)
{
cSort S = new cSort();
S.ColumnIndexForSorting = IdxColSorting;
S.SetInputData(TableForGeneralResuts);
S.IsAscending = true;
S.Run();
RT = S.GetOutPut();
}
cViewerTable VTForEC50 = new cViewerTable();
RT.Name = cGlobalInfo.CurrentScreening.ListDescriptors.GetActiveDescriptor().GetName();
VTForEC50.SetInputData(RT);
VTForEC50.DigitNumber = -1;
VTForEC50.Run();
MainSplitter.SetInputData(VTForEC50.GetOutPut());
MainSplitter.Orientation = Orientation.Vertical;
MainSplitter.Run();
cDisplayToWindow DTW = new cDisplayToWindow();
DTW.SetInputData(MainSplitter.GetOutPut());
DTW.Title = "DRC analysis [" + cGlobalInfo.CurrentScreening.ListDescriptors.GetActiveDescriptor().GetName() + "]";
DTW.Run();
DTW.Display();
}
void ToolStripMenuItem_DRCAnalysisMultiDesc_Click(object sender, EventArgs e)
{
List<cDescriptorType> ListSelectedDescs = cGlobalInfo.CurrentScreening.ListDescriptors.GetActiveDescriptors();
cListListWells CurrentSelectedGroups = this.BuildListListWells();
#region select folder
var dlg1 = new Ionic.Utils.FolderBrowserDialogEx();
dlg1.Description = "Select the folder containing your databases.";
dlg1.ShowNewFolderButton = true;
dlg1.ShowEditBox = true;
dlg1.ShowFullPathInEditBox = true;
DialogResult result = dlg1.ShowDialog();
if (result != DialogResult.OK) return;
string Path = dlg1.SelectedPath;
if (Directory.Exists(Path) == false) return;
string FolderName = dlg1.SelectedPath;
#endregion
foreach (var item in ListSelectedDescs)
{
string TmpFolder = FolderName + "\\" + item.GetName();
Directory.CreateDirectory(TmpFolder);
int IdxNode = 0;
List<cDescriptorType> ListSelectedDesc = new List<cDescriptorType>();
ListSelectedDesc.Add(item);
cExtendedTable TableForGeneralResuts = new cExtendedTable();
TableForGeneralResuts.Add(new cExtendedList("p-Value"));
TableForGeneralResuts.Add(new cExtendedList("EC50"));
TableForGeneralResuts.Add(new cExtendedList("Slope"));
TableForGeneralResuts.Add(new cExtendedList("Bottom"));
TableForGeneralResuts.Add(new cExtendedList("Top"));
TableForGeneralResuts.Add(new cExtendedList("Window"));
TableForGeneralResuts.Add(new cExtendedList("Area Under Curve"));
TableForGeneralResuts.ListRowNames = new List<string>();
TableForGeneralResuts.ListTags = new List<object>();
foreach (cListWells TmpListWells in CurrentSelectedGroups)
{
// List<cDescriptorType> LType = new List<cDescriptorType>();
// LType.Add(ListSelectedDesc[i]);
cExtendedTable CompleteTable = TmpListWells.GetAverageDescriptorValues(ListSelectedDesc, true, false);
//cExtendedTable CompleteTable = GLP.GetOutPut();
cCurveForGraph CFG = new cCurveForGraph();
CFG.SetInputData(CompleteTable);
CFG.Run();
cSigmoidFitting SF = new cSigmoidFitting();
SF.SetInputData(CompleteTable);
if (SF.Run().IsSucceed == false) continue;
// double Ratio = LR.GetOutPut()[0][LR.GetOutPut().Count - 1] / SF.GetOutPut()[0][SF.GetOutPut().Count - 1];
cANOVA A = new cANOVA();
cExtendedTable NewTable = CFG.ListPtValues.Crop(0, CFG.ListPtValues.Count - 1, 1, CFG.ListPtValues[0].Count - 1);
A.SignificanceThreshold = 1E-11;
A.SetInputData(NewTable);
A.Run();
cExtendedTable Sigmoid = SF.GetFittedRawValues(CFG.GetListXValues());
CompleteTable[0] = Sigmoid[1];
CompleteTable[0].Name = ListSelectedDesc[0].GetName() + "\n" + Sigmoid[1].Name;
cDesignerSplitter DS = new cDesignerSplitter();
//cViewerTableAsRichText VT = new cViewerTableAsRichText();
cViewerTable VT = new cViewerTable();
cExtendedTable TableResults = SF.GetOutPut();
if ((A.GetOutPut() != null) && (A.GetOutPut().Count > 0))
{
TableResults[0].Add(A.GetOutPut()[0][0]);
TableResults[0].Add(A.GetOutPut()[0][1]);
TableResults.ListRowNames.Add("p-Value");
TableResults.ListRowNames.Add("Rejected?");
TableForGeneralResuts[0].Add(A.GetOutPut()[0][0]);
}
else
{
TableForGeneralResuts[0].Add(1);
}
TableResults.Name = TV.Nodes[IdxNode].Text;
TableResults[0].Name = "Fitting Parameters";
VT.SetInputData(TableResults);
VT.DigitNumber = -1;
VT.Run();
cViewerGraph1D VS1 = new cViewerGraph1D();
cExtendedTable MyTable = new cExtendedTable(Sigmoid[1]);
MyTable.Name = TmpListWells.Name;// TV.Nodes[IdxNode].Text;
VS1.SetInputData(MyTable);
VS1.AddCurve(CFG);
VS1.Chart.X_AxisValues = Sigmoid[0];
VS1.Chart.IsLogAxis = true;
VS1.Chart.IsLine = true;
VS1.Chart.IsShadow = false;
VS1.Chart.Opacity = 210;
VS1.Chart.LineWidth = 3;
VS1.Chart.MarkerSize = 8;
VS1.Chart.IsDisplayValues = cGlobalInfo.OptionsWindow.FFAllOptions.checkBoxDRCDisplayValues.Checked;
VS1.Chart.LabelAxisX = "Concentration";
VS1.Chart.LabelAxisY = CompleteTable[1].Name;
VS1.Chart.XAxisFormatDigitNumber = cGlobalInfo.OptionsWindow.FFAllOptions.GetDRCNumberOfDigit();
VS1.Chart.IsZoomableX = true;
Classes.Base_Classes.General.cLineVerticalForGraph VLForEC50 = new Classes.Base_Classes.General.cLineVerticalForGraph(SF.GetOutPut()[0][2]);
//VLForEC50.AddText("EC50: " + SF.GetOutPut()[0][2].ToString("e3")/* + "\nError Ratio:" + Ratio.ToString("N4")*/);
VS1.Chart.ListVerticalLines.Add(VLForEC50);
TableForGeneralResuts.ListRowNames.Add(TV.Nodes[IdxNode].Text);
TableForGeneralResuts.ListTags.Add((Chart)VS1.Chart);//ListSelectedDesc[0]);
//EC50
TableForGeneralResuts[1].Add(SF.GetOutPut()[0][2]);
// Slope
TableForGeneralResuts[2].Add(SF.GetOutPut()[0][3]);
// Bottom
TableForGeneralResuts[3].Add(SF.GetOutPut()[0][0]);
// Top
TableForGeneralResuts[4].Add(SF.GetOutPut()[0][1]);
// Window
double Window = SF.GetOutPut()[0][1] / SF.GetOutPut()[0][0];
TableForGeneralResuts[5].Add(Window);
TableForGeneralResuts[6].Add(SF.GetOutPut()[0][5]);
VS1.Chart.ArraySeriesInfo = new cSerieInfoDesign[CompleteTable.Count];
for (int IdxCurve = 0; IdxCurve < CompleteTable.Count; IdxCurve++)
{
cSerieInfoDesign TmpSerieInfo = new cSerieInfoDesign();
TmpSerieInfo.color = cGlobalInfo.ListCellularPhenotypes[IdxCurve % cGlobalInfo.ListCellularPhenotypes.Count].ColourForDisplay;
TmpSerieInfo.markerStyle = MarkerStyle.None;
VS1.Chart.ArraySeriesInfo[IdxCurve] = TmpSerieInfo;
}
VS1.Run();
//DS.SetInputData(VS1.GetOutPut());
//DS.SetInputData(VT.GetOutPut());
//DS.Orientation = Orientation.Horizontal;
//DS.Title = TV.Nodes[IdxNode++].Text; //TmpListWells.ti;// ListSelectedDesc[0].GetName();
//DS.Run();
IdxNode++;
// DT.SetInputData(DS.GetOutPut());
}
cTableToHTML THTML = new cTableToHTML();
TableForGeneralResuts.Name = item.GetName();
THTML.SetInputData(TableForGeneralResuts);
THTML.IsDisplayUIForFilePath = false;
THTML.FolderName = TmpFolder;
THTML.ListProperties.FindByName("Open HTML File ?").SetNewValue((bool)false);
THTML.ListProperties.FindByName("Open HTML File ?").IsGUIforValue = false;
THTML.Run();
}
System.Diagnostics.Process.Start(FolderName);
}
private void ToolStripMenuItem_TestsOneWayANOVA(object sender, EventArgs e)
{
cExtendedTable CET = new cExtendedTable();
foreach (DataGridViewColumn item in GridView.SelectedColumns)
CET.Add(Input[item.Index]);
cANOVA S = new cANOVA();
S.SetInputData(CET);
S.Run();
cDisplayExtendedTable CDT = new cDisplayExtendedTable();
CDT.DigitNumber = -1;
CDT.SetInputData(S.GetOutPut());
CDT.Run();
}