public void Update(object sender, UpdateQuantificationItemEventArgs e)
{
var summary = e.Item as SilacQuantificationSummaryItem;
if (summary == null)
{
throw new ArgumentNullException("UpdateQuantificationItemEventArgs.Item cannot be null");
}
var pplRed = new PointPairList();
var pplGreen = new PointPairList();
var enabled = summary.ObservedEnvelopes.FindAll(m => m.Enabled);
enabled.ForEach(m =>
{
PointPairList ppl = m.IsSelected ? pplGreen : pplRed;
if (summary.SampleIsLight)
{
ppl.Add(new PointPair(m.HeavyIntensity, m.LightIntensity, 0.0, m));
}
else
{
ppl.Add(new PointPair(m.LightIntensity, m.HeavyIntensity, 0.0, m));
}
});
ZedGraphicExtension.ClearData(this.zgcGraph, false);
AddCurve(pplGreen, SilacQuantificationConstants.REFERENCE_COLOR);
AddCurve(pplRed, SilacQuantificationConstants.SAMPLE_COLOR);
var pplTotal = new PointPairList();
pplTotal.AddRange(pplRed);
pplTotal.AddRange(pplGreen);
if (pplTotal.Count > 0)
{
PointPairList line = ZedGraphicExtension.GetRegressionLine(pplTotal, summary.Ratio);
ZedGraphicExtension.AddDataToLine(this.zgcGraph, MyConvert.Format("Ratio = {0:0.0000}, Correlation = {1:0.0000}", summary.Ratio,
summary.RegressionCorrelation), line, SilacQuantificationConstants.IDENTIFIED_COLOR, false);
}
ZedGraphicExtension.UpdateGraph(this.zgcGraph);
}
public void Update(object sender, UpdateMRMPairedProductIonEventArgs e)
{
var summary = e.Item;
if (summary == null)
{
throw new ArgumentNullException("UpdateMRMItemEventArgs.Item cannot be null");
}
panel.Tag = e.Item;
panel.ClearData();
try
{
if (summary.Heavy != null)
{
var pplRed = new PointPairList();
var noise = summary.DeductBaseLine ? summary.Light.Noise : 0.0;
for (int i = 0; i < summary.Light.Intensities.Count; i++)
{
if (summary.Light.Intensities[i].Enabled)
{
pplRed.Add(new PointPair(summary.Heavy.Intensities[i].Intensity - noise, summary.Light.Intensities[i].Intensity - noise, 0.0, i));
}
}
;
if (pplRed.Count > 0)
{
panel.AddPoints(pplRed, Color.Red);
PointPairList line = ZedGraphicExtension.GetRegressionLine(pplRed, summary.Ratio, summary.Distance);
panel.AddCurve(null, line, Color.Green, SymbolType.None);
panel.Title.Text = summary.GetFormula();
panel.Title.IsVisible = true;
}
else
{
panel.Title.IsVisible = false;
}
}
}
finally
{
ZedGraphicExtension.UpdateGraph(this.zgcGraph);
}
}
public override void Update(object sender, UpdateQuantificationItemEventArgs e)
{
IExtendQuantificationSummaryOption option = e.Option as IExtendQuantificationSummaryOption;
string xTitle = string.Format("Log({0})", option.Func.ReferenceKey);
string yTitle = string.Format("Log({0})", option.Func.SampleKey);
panel.InitGraphPane(title, xTitle, yTitle, true, 0.0);
IIdentifiedProteinGroup group = e.Item as IIdentifiedProteinGroup;
try
{
Dictionary <string, List <PointPairList> > pplMap = new Dictionary <string, List <PointPairList> >();
var pplNormal = new PointPairList();
var pplSelected = new PointPairList();
var pplOutlier = new PointPairList();
var spectra = group.GetPeptides();
foreach (var pep in spectra)
{
if (pep.IsEnabled(true) && option.IsPeptideRatioValid(pep))
{
var key = option.GetPeptideClassification(pep);
if (!pplMap.ContainsKey(key))
{
var lst = new List <PointPairList>();
pplMap[key] = lst;
lst.Add(new PointPairList()); //selected
lst.Add(new PointPairList()); //outlier
lst.Add(new PointPairList()); //normal
}
var ppls = pplMap[key];
PointPairList ppl;
if (pep.Selected)
{
ppl = ppls[0];
}
else if (option.IsPeptideOutlier(pep))
{
ppl = ppls[1];
}
else
{
ppl = ppls[2];
}
double refIntensity = option.Func.GetReferenceIntensity(pep);
double sampleIntensity = option.Func.GetSampleIntensity(pep);
ppl.Add(refIntensity, sampleIntensity);
ppl[ppl.Count - 1].Tag = pep;
Debug.Assert(ppl[ppl.Count - 1].Tag == pep);
}
}
this.panel.ClearData();
foreach (var key in pplMap.Keys)
{
var ppls = pplMap[key];
this.panel.AddPoints(ppls[0], SelectedColor);
this.panel.AddPoints(ppls[1], NormalColor);
this.panel.AddPoints(ppls[2], OutlierColor);
var pplTotal = new PointPairList();
pplTotal.AddRange(ppls[0]);
pplTotal.AddRange(ppls[1]);
pplTotal.AddRange(ppls[2]);
if (pplTotal.Count > 0)
{
PointPairList line = ZedGraphicExtension.GetRegressionLine(pplTotal, option.GetProteinRatio(group[0], key));
var lineItem = this.panel.AddCurve(option.GetProteinRatioDescription(group[0], key), line, RegressionLineColor, SymbolType.None);
lineItem.Label.FontSpec = new FontSpec()
{
Size = 15, Border = new Border()
{
IsVisible = false
}
};
}
}
}
finally
{
ZedGraphicExtension.UpdateGraph(this.zgcGraph);
}
}
