// This method will be called when the thread is started.
public void Send_Results_Link(QuerryParameters p)
{
DBConnect db = new DBConnect();
string emailaddress = db.getEmail(p.userID);
using (MailMessage mm = new MailMessage("*****@*****.**", emailaddress))
{
mm.Subject = "Perceptron: Protein Search Results";
string body = "Hello " + p.userID + ",";
body += "<br /><br /> The results for protein search query submitted at " + "##-##-####" + " job title " + p.title + " have been computed. To see the results please click on following link(s).<br />";
for (int hijk = 0; hijk < p.peakListFile.Length; hijk++)
{
body += "<br />" + hijk.ToString() + ". Title: " + p.peakListFile[hijk].Substring(28 + p.userID.Length);
body += " <a href = \'http://*****:*****@gmail.com", "hawking1900");
smtp.UseDefaultCredentials = true;
smtp.Credentials = NetworkCred;
smtp.Port = 587;
smtp.Send(mm);
}
}
public void SetPriority(int n, QuerryParameters a)
{
if (n >= 0 && n < 3)
{
priority = n;
}
}
public static void ProteinSearch(QuerryParameters parameters)
{/////////prg
int location;
if (CurrentJobs.current == null)
{
location = 0;
}
else
{
location = CurrentJobs.current.Count;
}
Job newJob = new Job();
newJob.qid = parameters.queryid;
newJob.uid = parameters.userID;
newJob.progress = "Initiating Search!";//prg
CurrentJobs.Initialize();
CurrentJobs.current.Add(newJob);
////prg
int q = db.queryStore(parameters);
////////////////////////////////////////////////////////////////////////////////////
Worker workerObject = new Worker();
workerObject.location = location;
workerObject.parameters = parameters;
// Thread workerThread = new Thread(workerObject.DoWork);
//workerThread.Name = "searchThread";
//workerThread.Start();
workerObject.DoWork();
// return "ok";
}//function
public static void Entry(QuerryParameters p)
{
Attributes element = new Attributes();
element.querrpara = p;
element.priority = 0;
int vv;
vv = p.peakListFile.Length;
if (vv == 1)
{
medium.Add(element);
}
else
{
low.Add(element);
}
IA.checkQueuee();
}
//Returns Search History of specific query ie. search parameters and Results
public searchview retrieve_searchview_db(string qid)
{
searchview result = new searchview();
QuerryParameters qp = new QuerryParameters();
Results r = new Results();
string[] QIDArr = qid.Split('z');
qid = QIDArr[0];
int file_id = Convert.ToInt32(QIDArr[1]);
string query1 = "SELECT * FROM proteomics.query where QueryId='" + qid + "';";
string queryT = "SELECT * FROM proteomics.timings where querry_ID='" + qid + "';";
if (this.OpenConnection() == true)
{
try
{
using (MySqlTransaction trans = connection.BeginTransaction())
{
MySqlCommand cmd = new MySqlCommand(queryT, connection);
MySqlDataReader reader = cmd.ExecuteReader();
if (reader.Read())
{
r.times.insilico_time = reader.GetValue(1).ToString();
r.times.ptm_time = reader.GetValue(2).ToString();
r.times.tuner_time = reader.GetValue(3).ToString();
r.times.mw_filter_time = reader.GetValue(4).ToString();
r.times.pst_time = reader.GetValue(5).ToString();
r.times.total_time = reader.GetValue(6).ToString();
r.times.file = reader.GetValue(7).ToString();
}
reader.Close();
cmd = new MySqlCommand(query1, connection);
reader = cmd.ExecuteReader();
if (reader.Read())
{
qp.queryid = reader.GetValue(0).ToString();
qp.userID = reader.GetValue(1).ToString();
qp.title = reader.GetValue(2).ToString();
qp.protDB = reader.GetValue(3).ToString();
qp.outputFormat = Convert.ToInt32(reader.GetValue(4));
qp.insilico_frag_type = reader.GetValue(5).ToString();
qp.filterDB = Convert.ToInt32(reader.GetValue(6));
qp.ptm_tolerance = Convert.ToDouble(reader.GetValue(7));
qp.MWTolUnit = reader.GetValue(8).ToString();
qp.MW_tolerance = Convert.ToDouble(reader.GetValue(9));
qp.hopThreshhold = Convert.ToDouble(reader.GetValue(10));
qp.minimum_est_length = Convert.ToInt32(reader.GetValue(11));
qp.maximum_est_length = Convert.ToInt32(reader.GetValue(12));
// public double pst_tolerance;
qp.GUI_mass = Convert.ToDouble(reader.GetValue(13));
qp.HandleIons = reader.GetValue(14).ToString();
qp.autotune = Convert.ToInt32(reader.GetValue(15));
qp.peakListFile = reader.GetValue(16).ToString().Split('<');
qp.fileType = reader.GetValue(17).ToString();
// public double peptideTol;
//added
qp.hopTolUnit = reader.GetValue(18).ToString();
qp.MW_sweight = Convert.ToDouble(reader.GetValue(19));
qp.PST_sweight = Convert.ToDouble(reader.GetValue(20));
qp.Insilico_sweight = Convert.ToDouble(reader.GetValue(21));
qp.NumberOfOutputs = Convert.ToInt32(reader.GetValue(22));
/////
qp.denovo_allow = Convert.ToInt32(reader.GetValue(23));
qp.ptm_allow = Convert.ToInt32(reader.GetValue(24));
}
reader.Close();
query1 = "SELECT fixed_mod FROM fixed_modifications where QuerryId='" + qid + "';";
cmd = new MySqlCommand(query1, connection);
using (reader = cmd.ExecuteReader())
{
qp.ptm_code_fix = new List <int>();
while (reader.Read())
{
qp.ptm_code_fix.Add(Convert.ToInt32(reader["fixed_mod"]));
}
}
reader.Close();
query1 = "SELECT variable_mod FROM variable_modifications where QuerryId='" + qid + "';";
cmd = new MySqlCommand(query1, connection);
using (reader = cmd.ExecuteReader())
{
qp.ptm_code_var = new List <int>();
while (reader.Read())
{
qp.ptm_code_var.Add(Convert.ToInt32(reader["variable_mod"]));
}
}
reader.Close();
// SELECT * FROM proteomics.results Where Querry_ID = 'fe29e39e-97ee-4f5e-9cab-4d414b5e902a' ORDER BY score DESC LIMIT 3;
string query2 = "SELECT * FROM results where Querry_ID='" + qid + "' AND file_ID = " + file_id + " ORDER BY score DESC LIMIT " + qp.NumberOfOutputs + ";";
cmd = new MySqlCommand(query2, connection);
r.querryID = qid;
r.final_prot = new List <proteins>();
proteins temp_prot = new proteins();
List <string> resid = new List <string>();
using (reader = cmd.ExecuteReader())
{
while (reader.Read())
{
temp_prot = new proteins();
resid.Add(reader.GetValue(0).ToString());
temp_prot.header = reader.GetValue(2).ToString();
temp_prot.sequence = reader.GetValue(3).ToString();
temp_prot.est_score = Convert.ToDouble(reader.GetValue(4));
temp_prot.insilico_score = Convert.ToDouble(reader.GetValue(5));
temp_prot.ptm_score = Convert.ToDouble(reader.GetValue(6));
temp_prot.score = Convert.ToDouble(reader.GetValue(7));
temp_prot.MW_score = Convert.ToDouble(reader.GetValue(8));
temp_prot.MW = Convert.ToDouble(reader.GetValue(9));
r.final_prot.Add(temp_prot);
}
}
reader.Close();
Sites temp_site = new Sites();
string tempaa;
for (int i = 0; i < resid.Count; i++)
{
query2 = "SELECT * FROM ptm_sites where result_id='" + resid[i] + "';";
cmd = new MySqlCommand(query2, connection);
using (reader = cmd.ExecuteReader())
{
while (reader.Read())
{
temp_site = new Sites();
temp_site.i = Convert.ToInt32(reader.GetValue(1));
temp_site.score = Convert.ToInt32(reader.GetValue(2));
temp_site.mod_weight = Convert.ToInt32(reader.GetValue(3));
temp_site.mod_name = reader.GetValue(4).ToString();
temp_site.site = Convert.ToChar(reader.GetValue(5));
tempaa = reader.GetValue(6).ToString();
for (int j = 0; j < tempaa.Length; j++)
{
temp_site.AA.Add(tempaa[j]);
}
r.final_prot[i].ptm_particulars.Add(temp_site);
}
}
}
reader.Close();
///////////
//temp_p.insilico_details.insilico_mass_left = dataPs[3].Split(',').Select(t => double.Parse(t)).ToList<double>();
string xx;
for (int i = 0; i < resid.Count; i++)
{
query2 = "SELECT matched_peak_left FROM insilico_matches_left where result_id='" + resid[i] + "';";
cmd = new MySqlCommand(query2, connection);
using (reader = cmd.ExecuteReader())
{
temp_site = new Sites();
if (reader.Read())
{
xx = reader.GetValue(0).ToString();
r.final_prot[i].insilico_details.peaklist_mass_left = xx.Split(',').Select(t => double.Parse(t)).ToList <double>();
}
}
query2 = "SELECT Ions FROM leftions where ProteinID='" + r.final_prot[i].header + "';";
cmd = new MySqlCommand(query2, connection);
using (reader = cmd.ExecuteReader())
{
temp_site = new Sites();
if (reader.Read())
{
xx = reader.GetValue(0).ToString();
xx = Decompress(xx);
r.final_prot[i].insilico_details.insilico_mass_left = xx.Split(',').Select(t => double.Parse(t)).ToList <double>();
}
}
}
reader.Close();
//////////////////////////////////////////////////////////////
for (int i = 0; i < resid.Count; i++)
{
query2 = "SELECT matchedpeak_right FROM insilico_matches_right where result_id='" + resid[i] + "';";
cmd = new MySqlCommand(query2, connection);
using (reader = cmd.ExecuteReader())
{
temp_site = new Sites();
if (reader.Read())
{
xx = reader.GetValue(0).ToString();
r.final_prot[i].insilico_details.peaklist_mass_right = xx.Split(',').Select(t => double.Parse(t)).ToList <double>();
}
}
query2 = "SELECT Ions FROM rightions where ProteinID='" + r.final_prot[i].header + "';";
cmd = new MySqlCommand(query2, connection);
using (reader = cmd.ExecuteReader())
{
temp_site = new Sites();
if (reader.Read())
{
xx = reader.GetValue(0).ToString();
xx = Decompress(xx);
r.final_prot[i].insilico_details.insilico_mass_right = xx.Split(',').Select(t => double.Parse(t)).ToList <double>();
}
}
}
reader.Close();
//////////////////////////////////////////////////////////////
//for (int i = 0; i < resid.Count; i++)
//{
// query2 = "SELECT * FROM insilico_mass_right where result_id='" + resid[i] + "';";
// cmd = new MySqlCommand(query2, connection);
// using (reader = cmd.ExecuteReader())
// {
// while (reader.Read())
// {
// temp_site = new Sites();
// r.final_prot[i].insilico_details.insilico_mass_right.Add(Convert.ToDouble(reader.GetValue(1)));
// r.final_prot[i].insilico_details.peaklist_mass_right.Add(Convert.ToDouble(reader.GetValue(2)));
// }
// }
//}
//reader.Close();
////////////
result.param = qp;
result.result = r;
trans.Commit();
connection.Close();
}
}
catch (Exception e)
{
System.Diagnostics.Debug.WriteLine(e.Message);
}
}
// result.result.final_prot = result.result.final_prot.OrderByDescending(o => o.score).ToList();
return(result);
}
//Store Query parameters
public int queryStore(QuerryParameters param)
{
bool success = false;
if (this.OpenConnection() == true)
{
try
{
string filename = string.Join("<", param.peakListFile);
success = true;
string dateTime = DateTime.Now.ToString("yyyy-MM-dd HH:mm");
string query1 = "INSERT INTO query VALUES('" + param.queryid + "','" + param.userID + "','" + param.title + "','" + param.protDB + "','" + param.outputFormat + "','" + param.insilico_frag_type + "','" + param.filterDB + "','" + param.ptm_tolerance + "','"
+ param.MWTolUnit + "','" + param.MW_tolerance + "','" + param.hopThreshhold + "','" + param.minimum_est_length + "','" + param.maximum_est_length + "','" + param.GUI_mass + "','" + param.HandleIons + "'," + param.autotune + ",'" + filename + "','" + param.fileType +
"','" + param.hopTolUnit + "'," + param.MW_sweight + "," + param.PST_sweight + "," + param.Insilico_sweight + "," + param.NumberOfOutputs + "," + param.denovo_allow + "," + param.ptm_allow + ",'" + dateTime + "')";
MySqlCommand cmd = new MySqlCommand(query1, connection);
cmd.ExecuteNonQuery();
string query2;
for (int i = 0; i < param.ptm_code_var.Count; i++)
{
query2 = "INSERT INTO variable_modifications VALUES('" + param.queryid + "','" + param.ptm_code_var[i] + "')";
cmd = new MySqlCommand(query2, connection);
cmd.ExecuteNonQuery();
}
string query3;
for (int i = 0; i < param.ptm_code_fix.Count; i++)
{
query3 = "INSERT INTO fixed_modifications VALUES('" + param.queryid + "','" + param.ptm_code_fix[i] + "')";
cmd = new MySqlCommand(query3, connection);
cmd.ExecuteNonQuery();
}
string query6 = "INSERT INTO server_status VALUES('" + param.queryid + "','" + param.userID + "',0" + ")";
cmd = new MySqlCommand(query6, connection);
cmd.ExecuteNonQuery();
}
catch (Exception e)
{
string x = e.Message;
success = false;
}
}
this.CloseConnection();
if (success == false)
{
return(-1);
}
else
{
return(1);////
}
}
//Returns Search History of specific query ie. search Parameters and Results
public Searchview retrieve_searchview_db(string qid)
{
var result = new Searchview();
var qp = new QuerryParameters();
var res = new Results();
var qidArr = qid.Split('z');
qid = qidArr[0];
var fileId = Convert.ToInt32(qidArr[1]);
var query1 = "SELECT * FROM proteomics.query where QueryId='" + qid + "';";
var queryT = "SELECT * FROM proteomics.timings where querry_ID='" + qid + "';";
if (OpenConnection())
{
try
{
using (var trans = _connection.BeginTransaction())
{
var cmd = new MySqlCommand(queryT, _connection);
var reader = cmd.ExecuteReader();
if (reader.Read())
{
res.Times.InsilicoTime = reader.GetValue(1).ToString();
res.Times.PtmTime = reader.GetValue(2).ToString();
res.Times.TunerTime = reader.GetValue(3).ToString();
res.Times.MwFilterTime = reader.GetValue(4).ToString();
res.Times.PstTime = reader.GetValue(5).ToString();
res.Times.TotalTime = reader.GetValue(6).ToString();
res.Times.FileName = reader.GetValue(7).ToString();
}
reader.Close();
cmd = new MySqlCommand(query1, _connection);
reader = cmd.ExecuteReader();
if (reader.Read())
{
qp.QueryId = reader.GetValue(0).ToString();
qp.UserId = reader.GetValue(1).ToString();
qp.Title = reader.GetValue(2).ToString();
qp.ProtDb = reader.GetValue(3).ToString();
//qp.OutputFormat = Convert.ToInt32(reader.GetValue(4));
qp.InsilicoFragType = reader.GetValue(5).ToString();
qp.FilterDb = Convert.ToInt32(reader.GetValue(6));
qp.PtmTolerance = Convert.ToDouble(reader.GetValue(7));
qp.MwTolUnit = reader.GetValue(8).ToString();
qp.MwTolerance = Convert.ToDouble(reader.GetValue(9));
qp.HopThreshhold = Convert.ToDouble(reader.GetValue(10));
qp.MinimumEstLength = Convert.ToInt32(reader.GetValue(11));
qp.MaximumEstLength = Convert.ToInt32(reader.GetValue(12));
qp.GuiMass = Convert.ToDouble(reader.GetValue(13));
qp.HandleIons = reader.GetValue(14).ToString();
qp.Autotune = Convert.ToInt32(reader.GetValue(15));
qp.PeakListFile = reader.GetValue(16).ToString().Split('<');
qp.FileType = reader.GetValue(17).ToString();
qp.HopTolUnit = reader.GetValue(18).ToString();
qp.MwSweight = Convert.ToDouble(reader.GetValue(19));
qp.PstSweight = Convert.ToDouble(reader.GetValue(20));
qp.InsilicoSweight = Convert.ToDouble(reader.GetValue(21));
qp.NumberOfOutputs = Convert.ToInt32(reader.GetValue(22));
qp.DenovoAllow = Convert.ToInt32(reader.GetValue(23));
qp.PtmAllow = Convert.ToInt32(reader.GetValue(24));
qp.NeutralLoss = Convert.ToDouble(reader.GetValue(25)); //NeutralLoss Added!!!
qp.PSTTolerance = Convert.ToDouble(reader.GetValue(26)); //PSTTolerance Added!!!
}
reader.Close();
query1 = "SELECT fixed_mod FROM fixed_modifications where QueryId='" + qid + "';";
cmd = new MySqlCommand(query1, _connection);
using (reader = cmd.ExecuteReader())
{
qp.PtmCodeFix = new List <int>();
while (reader.Read())
{
qp.PtmCodeFix.Add(Convert.ToInt32(reader["fixed_mod"]));
}
}
reader.Close();
query1 = "SELECT variable_mod FROM variable_modifications where QueryId='" + qid + "';";
cmd = new MySqlCommand(query1, _connection);
using (reader = cmd.ExecuteReader())
{
qp.PtmCodeVar = new List <int>();
while (reader.Read())
{
qp.PtmCodeVar.Add(Convert.ToInt32(reader["variable_mod"]));
}
}
reader.Close();
var query2 = "SELECT * FROM results where Querry_ID='" + qid + "' AND file_ID = " + fileId +
" ORDER BY Score DESC LIMIT " + qp.NumberOfOutputs + ";";
cmd = new MySqlCommand(query2, _connection);
res.QueryId = qid;
res.FinalProt = new List <Proteins>();
var resid = new List <string>();
using (reader = cmd.ExecuteReader())
{
while (reader.Read())
{
var tempProt = new Proteins();
resid.Add(reader.GetValue(0).ToString());
tempProt.Header = reader.GetValue(2).ToString();
tempProt.Sequence = reader.GetValue(3).ToString();
tempProt.EstScore = Convert.ToDouble(reader.GetValue(4));
tempProt.InsilicoScore = Convert.ToDouble(reader.GetValue(5));
tempProt.PtmScore = Convert.ToDouble(reader.GetValue(6));
tempProt.Score = Convert.ToDouble(reader.GetValue(7));
tempProt.MwScore = Convert.ToDouble(reader.GetValue(8));
tempProt.Mw = Convert.ToDouble(reader.GetValue(9));
res.FinalProt.Add(tempProt);
}
}
reader.Close();
for (var i = 0; i < resid.Count; i++)
{
query2 = "SELECT * FROM ptm_sites where result_id='" + resid[i] + "';";
cmd = new MySqlCommand(query2, _connection);
using (reader = cmd.ExecuteReader())
{
while (reader.Read())
{
var tempSite = new Sites
{
Index = Convert.ToInt32(reader.GetValue(1)),
Score = Convert.ToInt32(reader.GetValue(2)),
ModWeight = Convert.ToInt32(reader.GetValue(3)),
ModName = reader.GetValue(4).ToString(),
Site = Convert.ToChar(reader.GetValue(5))
};
var tempaa = reader.GetValue(6).ToString();
foreach (char aminoacid in tempaa)
{
tempSite.AminoAcid.Add(aminoacid);
}
res.FinalProt[i].PtmParticulars.Add(tempSite);
}
}
}
reader.Close();
string xx;
for (var i = 0; i < resid.Count; i++)
{
query2 = "SELECT matched_peak_left FROM insilico_matches_left where result_id='" + resid[i] +
"';";
cmd = new MySqlCommand(query2, _connection);
using (reader = cmd.ExecuteReader())
{
if (reader.Read())
{
xx = reader.GetValue(0).ToString();
res.FinalProt[i].InsilicoDetails.PeaklistMassLeft =
xx.Split(',').Select(double.Parse).ToList();
}
}
query2 = "SELECT Ions FROM leftions where ProteinID='" + res.FinalProt[i].Header + "';";
cmd = new MySqlCommand(query2, _connection);
using (reader = cmd.ExecuteReader())
{
if (!reader.Read())
{
continue;
}
xx = reader.GetValue(0).ToString();
xx = Decompress(xx);
res.FinalProt[i].InsilicoDetails.InsilicoMassLeft =
xx.Split(',').Select(double.Parse).ToList();
}
}
reader.Close();
for (var i = 0; i < resid.Count; i++)
{
query2 = "SELECT matchedpeak_right FROM insilico_matches_right where result_id='" + resid[i] +
"';";
cmd = new MySqlCommand(query2, _connection);
using (reader = cmd.ExecuteReader())
{
if (reader.Read())
{
xx = reader.GetValue(0).ToString();
res.FinalProt[i].InsilicoDetails.PeaklistMassRight =
xx.Split(',').Select(double.Parse).ToList();
}
}
query2 = "SELECT Ions FROM rightions where ProteinID='" + res.FinalProt[i].Header + "';";
cmd = new MySqlCommand(query2, _connection);
using (reader = cmd.ExecuteReader())
{
if (reader.Read())
{
xx = reader.GetValue(0).ToString();
xx = Decompress(xx);
res.FinalProt[i].InsilicoDetails.InsilicoMassRight =
xx.Split(',').Select(double.Parse).ToList();
}
}
}
reader.Close();
result.Param = qp;
result.Result = res;
trans.Commit();
_connection.Close();
}
}
catch (Exception e)
{
Debug.WriteLine(e.Message);
}
return(result);
}
return(result);
}
protected async void Button2_Click(object sender, EventArgs e)
{
var fileNameArray = new string[File_Upload.PostedFiles.Count];
if (File_Upload.HasFile)
{
try
{
for (var i = 0; i < File_Upload.PostedFiles.Count; i++)
{
fileNameArray[i] = Path.Combine(@"C:\inetpub\wwwroot\shifaa\Peaklists\" + Session["ID"],
File_Upload.PostedFiles[i].FileName);
File_Upload.SaveAs(fileNameArray[i]);
}
var ext = Path.GetExtension(File_Upload.FileName);
if (Session["ID"] == null)
{
Response.Redirect("~/Account/Login", false);
}
var sessionId = Session["ID"];
if (sessionId != null)
{
var userId = sessionId.ToString();
var fixedMods = fixi.Value.Split(",".ToCharArray(), StringSplitOptions.RemoveEmptyEntries);
var variableMods = vari.Value.Split(",".ToCharArray(), StringSplitOptions.RemoveEmptyEntries);
var fixedItems = fixedMods.Select(mods => Convert.ToInt32(mods)).ToList();
var variableItems = variableMods.Select(mods => Convert.ToInt32(mods)).ToList();
var querryParameters = new QuerryParameters();
var qId = Guid.NewGuid();
Session["Qid"] = qId;
//General Info
querryParameters.userID = userId;
querryParameters.queryid = qId.ToString();
querryParameters.title = search_title.Text;
querryParameters.fileType = ext;
querryParameters.peakListFile = fileNameArray;
if (pro_mass.Text == "")
{
pro_mass.Text = "-1";
}
querryParameters.GUI_mass = Convert.ToDouble(pro_mass.Text);
querryParameters.protDB = database.Value;
querryParameters.NumberOfOutputs = Convert.ToInt32(No_of_outputs.Text);
////// MS/MS
querryParameters.insilico_frag_type = fragtype.Value;
querryParameters.HandleIons = ss.Value;
if (peptide_tol.Text == "")
{
peptide_tol.Text = "0.5";
}
querryParameters.hopThreshhold = Convert.ToDouble(peptide_tol.Text);
querryParameters.hopTolUnit = "Da";
//// Protein Filter
if (filterDB.Checked)
{
querryParameters.MWTolUnit = "Da";
if (protein_tol.Text == "")
{
protein_tol.Text = "100";
}
querryParameters.MW_tolerance = Convert.ToDouble(protein_tol.Text);
querryParameters.filterDB = 1;
}
else
{
querryParameters.MW_tolerance = 1000;
querryParameters.MWTolUnit = "Da";
querryParameters.filterDB = 0;
}
// Tuner
if (optionsRadios3.Checked)
{
if (!filterDB.Checked)
{
if (protein_tol.Text == "")
{
protein_tol.Text = "100";
}
querryParameters.MW_tolerance = Convert.ToDouble(protein_tol.Text);
}
querryParameters.autotune = 1;
}
else
{
querryParameters.autotune = 0;
}
//////// Denovo
if (optionsRadios5.Checked)
{
querryParameters.denovo_allow = 1;
////////// Length base
if (length.Checked)
{
querryParameters.maximum_est_length = Convert.ToInt32(PST_Length_max.SelectedItem.Value);
querryParameters.minimum_est_length = Convert.ToInt32(PST_Length_min.SelectedItem.Value);
}
else
{
querryParameters.maximum_est_length = -1;
querryParameters.minimum_est_length = -1;
}
}
else
{
querryParameters.denovo_allow = 0;
querryParameters.maximum_est_length = -1;
querryParameters.minimum_est_length = -1;
}
// PTM Filter
if (optionsRadios9.Checked)
{
querryParameters.ptm_allow = 1;
if (ptm_tol.Text == "")
{
ptm_tol.Text = "0.5";
}
querryParameters.ptm_tolerance = Convert.ToDouble(ptm_tol.Text);
querryParameters.ptm_code_fix = fixedItems;
querryParameters.ptm_code_var = variableItems;
if ((fixedItems.Count == 0) && (variableItems.Count == 0))
{
querryParameters.ptm_allow = 0;
}
}
else
{
querryParameters.ptm_allow = 0;
querryParameters.ptm_tolerance = -1;
fixedItems.Clear();
variableItems.Clear();
querryParameters.ptm_code_fix = fixedItems;
querryParameters.ptm_code_var = variableItems;
}
// Final weightage
if (optionsRadios11.Checked)
{
if (Insilico_Wei.Text == "")
{
Insilico_Wei.Text = "1";
}
if (MW_Wei.Text == "")
{
MW_Wei.Text = "1";
}
if (PST_Wei.Text == "")
{
PST_Wei.Text = "1";
}
querryParameters.Insilico_sweight = Convert.ToDouble(Insilico_Wei.Text);
querryParameters.MW_sweight = Convert.ToDouble(MW_Wei.Text);
querryParameters.PST_sweight = Convert.ToDouble(PST_Wei.Text);
}
else
{
querryParameters.Insilico_sweight = 1;
querryParameters.MW_sweight = 1;
querryParameters.PST_sweight = 1;
}
var paramContent = JsonConvert.SerializeObject(querryParameters);
var client = new HttpClient();
HttpContent httpCont = new StringContent(paramContent);
httpCont.Headers.ContentType = new MediaTypeHeaderValue("application/json");
const string uri = "http://localhost:14625//api/search/Post_search";
string result;
try
{
var getStringTask = client.PostAsync(uri, httpCont);
var xyz = await getStringTask;
result = xyz.Content.ReadAsStringAsync().Result;
Response.Redirect("~/jobQueue.aspx", false);
}
catch (Exception tt)
{
stat.Text = tt.Message;
result = "noty noty !";
}
}
}
catch (Exception w)
{
stat.Text = w.Message;
Debug.WriteLine(w.Message);
Debug.WriteLine(PST_Length_max.SelectedItem.Value);
optionsRadios3.Checked = false;
optionsRadios5.Checked = false;
optionsRadios9.Checked = false;
optionsRadios11.Checked = false;
filterDB.Checked = false;
length.Checked = false;
optionsRadios4.Checked = true;
optionsRadios6.Checked = true;
optionsRadios10.Checked = true;
optionsRadios12.Checked = true;
}
}
else
{
ClientScript.RegisterStartupScript(GetType(), "myalert", "alert('Please Uploa File to Continue!');",
true);
optionsRadios3.Checked = false;
optionsRadios5.Checked = false;
optionsRadios9.Checked = false;
optionsRadios11.Checked = false;
filterDB.Checked = false;
length.Checked = false;
optionsRadios4.Checked = true;
optionsRadios6.Checked = true;
optionsRadios10.Checked = true;
optionsRadios12.Checked = true;
}
}
public void Post_search([FromBody] QuerryParameters a)
{
UsersQueueMember.Entry(a);
}
public int GetPriority(QuerryParameters a)
{
return(priority);
}
