/// <summary>
/// Cut the last nucleotide of.
/// </summary>
/// <param name="minLen">Minimum primer length.</param>
/// <returns>Last nucleotide or 255 if oligo too short to pop.</returns>
public byte Pop(int minLen)
{
if (IsPopAllowed(minLen))
{
byte item = this.GeneSpecific[0];
this.GeneSpecific = this.GeneSpecific.GetSubSequence(0, this.GeneSpecific.Count - 1);
this.Sequence = new Sequence(Alphabets.DNA, Overlapping.ToString() + GeneSpecific.ToString());
this.PrimerTemperature = GetSimpleMeltingTemperature(GeneSpecific);
return(item);
}
else
{
return(255);
}
}
/// <summary>
/// Cut the first nucleotide off.
/// </summary>
/// <param name="minLen">Minimum overlap length.</param>
/// <returns>First nucleotide or 255 if oligo too short to dequeue.</returns>
public byte Dequeue(int minLen)
{
if (IsDequeAllowed(minLen))
{
byte item = this.Overlapping[this.Overlapping.Count - 1];
this.Overlapping = this.Overlapping.GetSubSequence(1, this.Overlapping.Count - 1);
this.Sequence = new Sequence(Alphabets.DNA, Overlapping.ToString() + GeneSpecific.ToString());
this.Temperature = GetSimpleMeltingTemperature(Overlapping);
return(item);
}
else
{
return(255);
}
}