public int[] DepthAtSites(IEnumerable <ICluster> clusters)
{
var depthAtSites = new int[0];
var veadgroups = clusters.SelectMany(x => x.GetVeadGroups());
return(VeadGroup.DepthAtSites(veadgroups));
}
public Agreement(VeadGroup vg1, VeadGroup vg2) : this()
{
var read1 = vg1.RepresentativeVead;
var read2 = vg2.RepresentativeVead;
for (var i = 0; i < read1.SiteResults.Length; i++)
{
var vs1 = read1.SiteResults[i];
var vs2 = read2.SiteResults[i];
if ((vs1.VcfAlternateAllele == "N") || (vs2.VcfAlternateAllele == "N"))
{
continue;
}
if ((vs1.VcfAlternateAllele == vs2.VcfAlternateAllele) && (vs1.VcfReferenceAllele == vs2.VcfReferenceAllele))
{
NumAgreement++;
}
else
{
NumDisagreement++;
}
}
}
public void CreateAndAddCluster(VeadGroup veadGroup)
{
var clusterName = "#" + (NumClusters + 1);
var cluster = new Cluster(clusterName, new List <VeadGroup> {
veadGroup
});
AddCluster(cluster);
}
public void Add(VeadGroup vg, bool updateConsensus = true)
{
_veadGroups.Add(vg);
if (!Closed && updateConsensus)
{
UpdateConsensus(vg);
}
}
private void UpdateConsensus(VeadGroup rg)
{
if (_consensus == null)
{
return;
}
//TODO why would you ever have more known refs than alts or vice versa?
VeadGroupMerger.MergeProfile1Into2(rg.SiteResults, _consensus.SiteResults);
_numKnownRef = _consensus.SiteResults.Count(s => s.HasRefData());
_numKnownAlt = _consensus.SiteResults.Count(s => s.HasAltData());
}
public SortedDictionary <int, List <Cluster> > GetClusterFits(VeadGroup r1)
{
var bestFits = new SortedDictionary <int, List <Cluster> >();
for (var clusterIndex = 0; clusterIndex < NumClusters; clusterIndex++)
{
var cluster = Clusters[clusterIndex];
var bestAgreementWithThiscluster = cluster.GetBestAgreementWithVeadGroup(r1, _clusterParameters.MaxNumberDisagreements);
//not allowed to connect this read!
if (!AgreementIsSufficient(bestAgreementWithThiscluster, _clusterParameters.MaxNumberDisagreements, _clusterParameters.MinNumberAgreements))
{
continue;
}
if (!bestFits.ContainsKey(bestAgreementWithThiscluster.Score))
{
bestFits.Add(bestAgreementWithThiscluster.Score, new List <Cluster>());
}
bestFits[bestAgreementWithThiscluster.Score].Add(cluster);
if ((bestFits.Count <= 1) && (bestFits[bestAgreementWithThiscluster.Score].Count <= 1))
{
continue;
}
//TODO, when debug enabled.
/*
* WriteToClusterLog("investigate!!");
* WriteToClusterLog("found a read that can join two clusters!");
* WriteToClusterLog("read group: " + r1.ToString());
* WriteToClusterLog("bestFits.Count: " + bestFits.Count);
* WriteToClusterLog("num best scoring : " + bestFits[bestAgreementWithThiscluster.Score].Count);
*
* foreach (var c in bestFits[bestAgreementWithThiscluster.Score])
* {
* WriteToClusterLog("\t" + c.Name);
* }*/
}
return(bestFits);
}
private Agreement GetAgreementWithCluster(VeadGroup v1)
{
var netAgreement = new Agreement();
for (var i = 0; i < NumVeadGroups; i++)
{
var v2 = _veadGroups[i];
if (v1 == v2)
{
continue;
}
var a = new Agreement(v1, v2);
netAgreement.AddAgreement(a);
}
return(netAgreement);
}
public void ResetConsensus()
{
_consensus = null;
_numKnownAlt = 0;
_numKnownRef = 0;
if ((_veadGroups == null) || (_veadGroups.Count == 0))
{
return;
}
var vead = new Vead(Name, _veadGroups[0].SiteResults);
_consensus = new VeadGroup(vead);
foreach (var rg in _veadGroups.Where(rg => !Closed))
{
UpdateConsensus(rg);
}
}
public Agreement GetBestAgreementWithVeadGroup(VeadGroup newVeadGroup, int maxNumberDisagreements)
{
var bestAgreement = new Agreement();
foreach (var agreement in _veadGroups.Where(vg => newVeadGroup.Name != vg.Name).
Select(vg => new Agreement(newVeadGroup, vg)))
{
//we disagree with something in the cluster already.
if (agreement.NumDisagreement > maxNumberDisagreements)
{
return(null);
}
if (agreement.CompareTo(bestAgreement) > 0)
{
bestAgreement = agreement;
}
}
return(bestAgreement);
}
public VeadGroup GetWorstAgreement()
{
// There will always be a "worst" unless there are no vead groups...
VeadGroup worstVeadGroup = null;
var worstAgreement = new Agreement {
NumAgreement = int.MaxValue, NumDisagreement = 0
};
for (var i = 0; i < NumVeadGroups; i++)
{
var agreementWithCluster = GetAgreementWithCluster(_veadGroups[i]);
if (agreementWithCluster.CompareTo(worstAgreement) >= 0)
{
continue;
}
worstAgreement = agreementWithCluster;
worstVeadGroup = _veadGroups[i];
}
return(worstVeadGroup);
}
public void DepthAtSites(IEnumerable <ICluster> clusters, out int[] depths, out int[] nocalls)
{
var veadgroups = clusters.SelectMany(x => x.GetVeadGroups());
VeadGroup.DepthAtSites(veadgroups, out depths, out nocalls);
}
public void Remove(VeadGroup vg)
{
_veadGroups.Remove(vg);
ResetConsensus();
}
public int GetClusterReferenceSupport(IEnumerable <ICluster> clusters)
{
var clusterVead = new Vead("test", GetConsensusSites());
var numRef = 0;
foreach (VariantSite vs in clusterVead.SiteResults)
{
// Nima: If cluster contains reference or X>X at this site, this site won't be included in the MNV.
// So veads that support reference for this cluster can hold any variant at these sites (hence using N>N).
// In the following example, cluster reference support for Cluster3 includes both Cluster1 and Cluster4
//---------------------------------------
// Sites S1 S2 S3
// Cluster1 R R R
// Cluster2 R R X
// Cluster3 R X X
// Cluster4 X R R
//----------------------------------------
//Cluster3's refVead N>N R>R R>R
//
// However, if the cluster is all ref we should avoid all N>N, and instead have the original R>R
// In example above:
//Cluster1's refVead R>R R>R R>R
if (vs.IsReference || vs.VcfAlternateAllele == vs.VcfReferenceAllele)
{
vs.VcfReferenceAllele = "N";
vs.VcfAlternateAllele = "N";
if (vs.IsReference)
{
numRef++;
}
}
else // If cluster contains a variant at this site, ref veads need to contain ref>ref at this site.
{
vs.VcfReferenceAllele = vs.VcfReferenceAllele[0].ToString();
vs.VcfAlternateAllele = vs.VcfReferenceAllele;
}
}
var refVead = clusterVead;
// Check if all variants were reference, assign original consensus to refVead
if (numRef == clusterVead.SiteResults.Count())
{
refVead = new Vead("test", GetConsensusSites());
}
var clusterRefVeadGroup = new VeadGroup(refVead);
var numVariants = clusterRefVeadGroup.NumSitesPerVead;
var cluterRefSupport = 0;
foreach (var cls in clusters)
{
List <VeadGroup> vgs = cls.GetVeadGroups();
foreach (var vg in vgs)
{
var agreement = new Agreement(vg, clusterRefVeadGroup);
if (agreement.NumDisagreement == 0 &&
agreement.NumAgreement >= numVariants)
{
cluterRefSupport += vg.NumVeads;
}
}
}
return(cluterRefSupport);
}