private void resetButton_Click(object sender, EventArgs e)
{
resetButton.Enabled = false;
context.Visible = true;
helpLabel.Visible = true;
successfullyCut = false;
enzymeCombo.Enabled = true;
cutSiteLabel.Visible = true;
pushClockwiseButton.Enabled = true;
pushCounterclockwiseButton.Enabled = true;
cutBeginningButton.Enabled = true;
cutEndButton.Enabled = true;
cornerBox2.Top -= baseDisplaySize * 3;
cornerBox1.Visible = true;
cornerBox3.Visible = true;
cornerBox4.Visible = true;
string newDNA;
switch (newDNAcombo.SelectedIndex)
{
case 0:
newDNA = pAMP_DNA;
break;
case 1:
newDNA = pKAN_DNA;
break;
case 2:
newDNA = humira_DNA;
break;
case 3:
newDNA = humulin_DNA;
break;
default:
newDNA = pAMP_DNA;
break;
}
plasmid = BasePair.createFromString(newDNA);
//DNA_Index = startPicker.Next(plasmid.Length);
DNA_Index = 0;
chosenGene = newDNAcombo.Text;
if (chosenGene == "Humira" || chosenGene == "Humulin")
{
completeLoop = false;
}
if (chosenGene == "pKAN" || chosenGene == "pAMP")
{
completeLoop = true;
}
cutBeginning = -1;
cutEnd = -1;
displayPanel.Refresh();
}
public static BasePair[] createFromStringBottom(string dna)
{
BasePair[] returnValue = new BasePair[dna.Length];
int i = 0;
foreach (char value in dna)
{
returnValue[i] = new BasePair(value, false);
i++;
}
return(returnValue);
}
public geneCutter()
{
InitializeComponent();
this.Icon = Icon.ExtractAssociatedIcon(Application.ExecutablePath);
this.Text = "Gene Merger | " + Globals.name + " - " + Globals.period;
plasmid = BasePair.createFromString(pAMP_DNA);
enzymeCombo.SelectedIndex = 0;
//DNA_Index = startPicker.Next(plasmid.Length);
DNA_Index = 0;
newDNAcombo.SelectedIndex = 0;
chosenGene = "pAMP";
ecoAMPcheck.Checked = Globals.ecoAMPcut;
bamAMPcheck.Checked = Globals.bamAMPcut;
ecoKANcheck.Checked = Globals.ecoKANcut;
bamKANcheck.Checked = Globals.bamKANcut;
ecoHumulinCheck.Checked = Globals.ecoHumulinCut;
bamHumulinCheck.Checked = Globals.bamHumulinCut;
ecoHumiraCheck.Checked = Globals.ecoHumiraCut;
bamHumiraCheck.Checked = Globals.bamHumiraCut;
resetButton.Enabled = false;
}
private void insertGene(object sender, System.EventArgs e)
{
Button clickedButton = (Button)sender;
if (clickedButton.Name.Contains("1"))
{
switch (clickedButton.Name.Substring(0, clickedButton.Name.Length - 1))
{
case "ecoAMPbutton":
leftCutSite = ecoCutSite;
leftPlasmid = "ecoAMP";
leftTop = BasePair.createFromString(Globals.ecoAMPcutSite.Substring(0, 8));
leftBottom = BasePair.createFromString(Globals.ecoAMPcutSite.Substring(3, Globals.ecoAMPcutSite.Length - 3));
break;
case "bamAMPbutton":
leftCutSite = bamCutSite;
leftPlasmid = "bamAMP";
leftTop = BasePair.createFromString(Globals.bamAMPcutSite.Substring(0, 8));
leftBottom = BasePair.createFromString(Globals.bamAMPcutSite.Substring(3, Globals.bamAMPcutSite.Length - 3));
break;
case "ecoKANbutton":
leftCutSite = ecoCutSite;
leftPlasmid = "ecoKAN";
leftTop = BasePair.createFromString(Globals.ecoKANcutSite.Substring(0, 8));
leftBottom = BasePair.createFromString(Globals.ecoKANcutSite.Substring(3, Globals.ecoKANcutSite.Length - 3));
break;
case "bamKANbutton":
leftCutSite = bamCutSite;
leftPlasmid = "bamKAN";
leftTop = BasePair.createFromString(Globals.bamKANcutSite.Substring(0, 8));
leftBottom = BasePair.createFromString(Globals.bamKANcutSite.Substring(3, Globals.bamKANcutSite.Length - 3));
break;
case "ecoHumiraButton":
leftPlasmid = "ecoHumira";
leftCutSite = ecoCutSite;
leftTop = BasePair.createFromString(Globals.ecoHumiraCutSite.Substring(0, 8));
leftBottom = BasePair.createFromString(Globals.ecoHumiraCutSite.Substring(3, Globals.ecoHumiraCutSite.Length - 3));
break;
case "bamHumiraButton":
leftPlasmid = "bamHumira";
leftCutSite = bamCutSite;
leftTop = BasePair.createFromString(Globals.bamHumiraCutSite.Substring(0, 8));
leftBottom = BasePair.createFromString(Globals.bamHumiraCutSite.Substring(3, Globals.bamHumiraCutSite.Length - 3));
break;
case "ecoHumulinButton":
leftPlasmid = "ecoHumulin";
leftCutSite = ecoCutSite;
leftTop = BasePair.createFromString(Globals.ecoHumulinCutSite.Substring(0, 8));
leftBottom = BasePair.createFromString(Globals.ecoHumulinCutSite.Substring(3, Globals.ecoHumulinCutSite.Length - 3));
break;
case "bamHumulinButton":
leftPlasmid = "bamHumulin";
leftCutSite = bamCutSite;
leftTop = BasePair.createFromString(Globals.bamHumulinCutSite.Substring(0, 8));
leftBottom = BasePair.createFromString(Globals.bamHumulinCutSite.Substring(3, Globals.bamHumulinCutSite.Length - 3));
break;
}
}
else if (clickedButton.Name.Contains("2"))
{
switch (clickedButton.Name.Substring(0, clickedButton.Name.Length - 1))
{
case "ecoAMPbutton":
rightCutSite = ecoCutSite;
rightPlasmid = "ecoAMP";
rightTop = BasePair.createFromString(Globals.ecoAMPcutSite.Substring(0, 8));
rightBottom = BasePair.createFromString(Globals.ecoAMPcutSite.Substring(3, Globals.ecoAMPcutSite.Length - 3));
break;
case "bamAMPbutton":
rightCutSite = bamCutSite;
rightPlasmid = "bamAMP";
rightTop = BasePair.createFromString(Globals.bamAMPcutSite.Substring(0, 8));
rightBottom = BasePair.createFromString(Globals.bamAMPcutSite.Substring(3, Globals.bamAMPcutSite.Length - 3));
break;
case "ecoKANbutton":
rightCutSite = ecoCutSite;
rightPlasmid = "ecoKAN";
rightTop = BasePair.createFromString(Globals.ecoKANcutSite.Substring(0, 8));
rightBottom = BasePair.createFromString(Globals.ecoKANcutSite.Substring(3, Globals.ecoKANcutSite.Length - 3));
break;
case "bamKANbutton":
rightCutSite = bamCutSite;
rightPlasmid = "bamKAN";
rightTop = BasePair.createFromString(Globals.bamKANcutSite.Substring(0, 8));
rightBottom = BasePair.createFromString(Globals.bamKANcutSite.Substring(3, Globals.bamKANcutSite.Length - 3));
break;
case "ecoHumiraButton":
rightPlasmid = "ecoHumira";
rightCutSite = ecoCutSite;
rightTop = BasePair.createFromString(Globals.ecoHumiraCutSite.Substring(0, 8));
rightBottom = BasePair.createFromString(Globals.ecoHumiraCutSite.Substring(3, Globals.ecoHumiraCutSite.Length - 3));
break;
case "bamHumiraButton":
rightPlasmid = "bamHumira";
rightCutSite = bamCutSite;
rightTop = BasePair.createFromString(Globals.bamHumiraCutSite.Substring(0, 8));
rightBottom = BasePair.createFromString(Globals.bamHumiraCutSite.Substring(3, Globals.bamHumiraCutSite.Length - 3));
break;
case "ecoHumulinButton":
rightPlasmid = "ecoHumulin";
rightCutSite = ecoCutSite;
rightTop = BasePair.createFromString(Globals.ecoHumulinCutSite.Substring(0, 8));
rightBottom = BasePair.createFromString(Globals.ecoHumulinCutSite.Substring(3, Globals.ecoHumulinCutSite.Length - 3));
break;
case "bamHumulinButton":
rightPlasmid = "bamHumulin";
rightCutSite = bamCutSite;
rightTop = BasePair.createFromString(Globals.bamHumulinCutSite.Substring(0, 8));
rightBottom = BasePair.createFromString(Globals.bamHumulinCutSite.Substring(3, Globals.bamHumulinCutSite.Length - 3));
break;
}
}
combinePanel.Refresh();
if (leftTop != null && leftBottom != null && rightTop != null && rightBottom != null)
{
merge();
}
}
