private void ProcessSliceOnce(List<POINT> ptsAspOrg, List<double> volumes, string liquidClass ,
int srcRackIndex,int sliceIndex, int sampleIndexInRack,StreamWriter sw)
{
//有时候，液体需要被喷到不同行，比如目标载架为4*6时
bool inSameColumn = IsDstWellsInSameColumn(srcRackIndex, sampleIndexInRack, ptsAspOrg.Count);
int srcGrid = GetSrcGrid(srcRackIndex);
int globalSampleIndex = GetGlobalSampleIndex(srcRackIndex, sampleIndexInRack); //
bool bNeedUseLastFour = NeedUseLastFour(sampleIndexInRack);
List<POINT> ptsAsp = new List<POINT>(ptsAspOrg);
if (bNeedUseLastFour)
{
POINT ptZero = new POINT(0, 0);
ptsAsp.InsertRange(0, new List<POINT>() { ptZero, ptZero, ptZero, ptZero });
volumes.InsertRange(0, new List<double>() { 0, 0, 0, 0 });
}
//2 吸，喷
string strAspirate = GenerateAspirateCommand(ptsAsp, volumes, liquidClass, srcGrid,0, labwareSettings.sourceWells);
sw.WriteLine(strAspirate);
if (inSameColumn)
{
List<POINT> ptsDisp = positionGenerator.GetDestWells(srcRackIndex, sliceIndex, sampleIndexInRack, ptsAspOrg.Count);
int grid = 0, site = 0;
CalculateDestGridAndSite(globalSampleIndex, sliceIndex, ref grid, ref site);
if(bNeedUseLastFour)
{
POINT ptZero = new POINT(0, 0);
ptsDisp.InsertRange(0, new List<POINT>() { ptZero, ptZero, ptZero, ptZero });
}
string strDispense = GenerateDispenseCommand(ptsDisp, volumes, liquidClass, grid,site, labwareSettings.dstLabwareRows);
sw.WriteLine(strDispense);
}
else
{
//throw new Exception("try to dispense to different regions.");
int endIndexFirstColumn = GetEndIndexForFirstColumn(srcRackIndex, sampleIndexInRack);
int firstColumnSampleCount = endIndexFirstColumn - srcRackIndex * labwareSettings.sourceWells - sampleIndexInRack + 1;
List<POINT> ptsDisp = positionGenerator.GetDestWells(srcRackIndex,sliceIndex, sampleIndexInRack, firstColumnSampleCount);
int grid = 0, site = 0;
CalculateDestGridAndSite4OneSlicePerLabware(sliceIndex, ref grid, ref site);
List<double> volumes1;
List<double> volumes2;
SplitVolumes2Region(volumes, out volumes1, out volumes2, firstColumnSampleCount);
string strDispense = GenerateDispenseCommand(ptsDisp, volumes1, liquidClass, grid, site, labwareSettings.dstLabwareRows);
sw.WriteLine(strDispense);
int secondColumnStartSampleIndex = endIndexFirstColumn + 1;
ptsDisp = positionGenerator.GetDestWells(srcRackIndex,sliceIndex, sampleIndexInRack + firstColumnSampleCount, ptsAsp.Count - firstColumnSampleCount);
strDispense = GenerateDispenseCommand(ptsDisp, volumes2, liquidClass, grid, site, labwareSettings.dstLabwareRows);
sw.WriteLine(strDispense);
}
}
private void WriteDispenseBuffyNoCheck(List<POINT> pts, int grid,int site, StreamWriter sw, int tipOffset)
{
log.Info("WriteDispenseBuffy for certain region");
int sampleCnt = pts.Count;
int ditiMask = GetTipSelection(sampleCnt,tipOffset);
List<double> vols = new List<double>();
for(int i = 0; i < 8; i++)
{
vols.Add(0);
}
for( int i = 0; i< sampleCnt;i++)
{
if (tipOffset + i < 8)
vols[tipOffset + i] = 10;
}
WriteDispenseBuffyWithMovingPluger(pts,ditiMask,vols,grid,site,tipOffset,sw);
//List<POINT> pts = positionGenerator.GetDestWellsForCertainSliceOfOneBatch(batchIndex,0,false);
//WriteComment("Move tips up.", sw);
//sw.WriteLine(sMoveLiha);
List<double> volumes = new List<double>();
//int startTip = 0;
int buffySlice = pipettingSetting.dstbuffySlice;
if (buffySlice > 1) //need dispense the buffy
{
volumes.Clear();
for (int i = 0; i < tipOffset + sampleCnt; i++)
{
if (i < tipOffset || buffySlice == 0)
volumes.Add(0);
else
{
volumes.Add(pipettingSetting.buffyVolume * (buffySlice - 1) / buffySlice);
}
}
WriteComment("aspirate buffy from slice 1", sw);
if(tipOffset == 4) //use last four
{
POINT ptZero = new POINT(0, 0);
pts.InsertRange(0, new List<POINT> { ptZero, ptZero, ptZero, ptZero });
}
string strAsp = GenerateAspirateCommand(pts, volumes, BB_Buffy_Mix, grid, site, labwareSettings.dstLabwareRows);
sw.WriteLine(strAsp);
for (int i = 0; i < volumes.Count; i++)
volumes[i] = volumes[i] / (buffySlice - 1);
List<POINT> ptsDisp = new List<POINT>(pts);
for (int slice = 1; slice < pipettingSetting.dstbuffySlice; slice++)
{
int sliceUsedGrid = 0;
if (labwareSettings.dstLabwareColumns == 1)
sliceUsedGrid = slice;
else
{
ptsDisp = ChangePositions(pts, slice);
}
string strDispense = GenerateDispenseCommand(ptsDisp, volumes, BB_Buffy_Mix, grid + sliceUsedGrid, site, labwareSettings.dstLabwareRows);
WriteComment(string.Format("Dispensing buffy slice: {0}", slice + 1), sw);
sw.WriteLine(strDispense);
}
}
}
private void GenerateForBatch(string sOutput,int rackIndex, int sampleIndexInRack, List<DetectedInfo> heightsThisTime)
{
bool bNeedUseLastFour = NeedUseLastFour(sampleIndexInRack);
log.InfoFormat("rack index : {0}, start sample : {1}", rackIndex, sampleIndexInRack);
//batch index
int batchID = 1+ sampleIndexInRack / labwareSettings.tipCount;
int tipOffSet = GetTipOffSet(bNeedUseLastFour);
int ditiMask = 0;
for (int i = tipOffSet; i < tipOffSet + heightsThisTime.Count; i++)
ditiMask += (int)Math.Pow(2, i);
//create batch file
string suffix = ".gwl";
string sBatchFile = sOutput + string.Format("\\worklist{0}{1}",batchID,suffix);
if (File.Exists(sBatchFile))
File.Delete(sBatchFile);
FileStream fs = new FileStream(sBatchFile, FileMode.CreateNew);
StreamWriter sw = new StreamWriter(fs, Encoding.Default);
sw.WriteLine("W;");
string sNotifierFolder = Utility.GetExeFolder();
//0 get diti
WriteComment(string.Format("batch id is: {0}", batchID), sw);
sw.WriteLine(string.Format(breakPrefix + "GetDiti2({0},\"DiTi 1000ul LiHa\",0,0,10,70);", ditiMask));
//1 aspirate plasmas
double area = mappingCalculator.GetArea();
if(GlobalVars.Instance.IsRedCell)
{
pipettingSetting.dstbuffySlice = 0;
mixCommand mixCommand = new mixCommand();
List<int> vols = new List<int>();
heightsThisTime.ForEach(x=>vols.Add((int)(mappingCalculator.GetVolumeFromHeight(x.Z1)-mappingCalculator.GetVolumeFromHeight(x.Z2))));
var strs = mixCommand.GenerateMixForBatch(rackIndex,sampleIndexInRack,vols);
strs.ForEach(s => sw.WriteLine(s));
}
List<POINT> ptsAsp = positionGenerator.GetSrcWells(sampleIndexInRack, heightsThisTime.Count); //.GetSrcWellsForCertainSliceOfOneBatch(batchIndex);
int plasmaSlice = pipettingSetting.dstPlasmaSlice;
List<double> plasmaVols = new List<double>();
for (int slice = 0; slice < plasmaSlice; slice++)
{
string sExe = sNotifierFolder + string.Format("Notifier.exe Pipetting;{0};{1};{2}", rackIndex,batchID-1, slice);
if(sNotifierFolder != "")
sw.WriteLine(string.Format(breakPrefix + "Execute(\"{0}\",2,\"\",2);", sExe));
WriteComment(string.Format("Processing slice: {0}, plasma part", slice + 1), sw);
plasmaVols = GenerateForSlice(slice, plasmaSlice,ptsAsp,rackIndex,sampleIndexInRack, heightsThisTime,sw);
if(GlobalVars.Instance.TrackBarcode)
barcodeTracker.Track(plasmaVols,slice);
}
//2 aspirate & dispense buffy
bool bhasBuffyCoat = pipettingSetting.dstbuffySlice > 0;//ResultReader.Instance.HasBuffyCoat();
bool inSameColumn = IsDstWellsInSameColumn(rackIndex, sampleIndexInRack, ptsAsp.Count);
int globalSampleIndex = GetGlobalSampleIndex(rackIndex, sampleIndexInRack);
//int secondRegionStartSampleIndex = GetEndIndexForFirstRegion(rackIndex, startSample) + 1;
if (bhasBuffyCoat)
{
string sExe = sNotifierFolder + string.Format("Notifier.exe Pipetting;{0};{1};{2}", rackIndex,batchID-1, pipettingSetting.dstPlasmaSlice);
if(sNotifierFolder != "")
sw.WriteLine(string.Format(breakPrefix + "Execute(\"{0}\",2,\"\",2);", sExe));
//3 为buffy设置tipVolume
List<double> buffyvolumes = new List<double>();
if(bNeedUseLastFour)
{
buffyvolumes.AddRange(new List<double> { 0, 0, 0, 0 });
POINT ptZero = new POINT(0, 0);
ptsAsp.InsertRange(0, new List<POINT>() { ptZero, ptZero, ptZero, ptZero });
}
for (int tipIndex = 0; tipIndex < heightsThisTime.Count; tipIndex++)
{
buffyvolumes.Add(10); //为了让sample tracking起作用，加10ul
}
int srcGrid = GetSrcGrid(rackIndex);
string strAspirateBuffy = GenerateAspirateCommand(ptsAsp, buffyvolumes, BB_Buffy, srcGrid, 0, labwareSettings.sourceWells);
sw.WriteLine(strAspirateBuffy);
//4 asp buffy using MSD
WriteMSDCommands(sw, heightsThisTime, tipOffSet);
//5 dispense buffy
if (inSameColumn)
{
WriteDispenseBuffy(rackIndex, sampleIndexInRack, heightsThisTime.Count,bNeedUseLastFour,false, sw);
}
else//need to dispense to different column
{
int endIndexFirstColumn = GetEndIndexForFirstColumn(rackIndex, sampleIndexInRack);
int firstColumnSampleCount = endIndexFirstColumn - rackIndex * labwareSettings.sourceWells - sampleIndexInRack + 1;
WriteDispenseBuffy(rackIndex, sampleIndexInRack, firstColumnSampleCount, bNeedUseLastFour, true, sw);
int secondColumnStartSampleIndex = endIndexFirstColumn + 1;
WriteDispenseBuffy(rackIndex, secondColumnStartSampleIndex, heightsThisTime.Count - firstColumnSampleCount, bNeedUseLastFour, true, sw);
}
}
sw.WriteLine(string.Format(breakPrefix + "DropDiti({0},{1},2,10,70,0);", ditiMask,labwareSettings.wasteGrid));
int endSampleID = rackIndex * 16 + sampleIndexInRack + heightsThisTime.Count;
if (endSampleID >= detectInfos.Count)
{
string sFinishedCommand = sNotifierFolder + string.Format("Notifier.exe Pipetting;true");
sw.WriteLine(string.Format(breakPrefix + "Execute(\"{0}\",2,\"\",2);", sFinishedCommand));
}
sw.Close();
}
