/// <summary>
/// Analyse image file - crop with tracking, copy Polygonal ROI, apply Sobel operator and save the results
/// </summary>
/// <param name="DirData">Output and input directories </param>
/// <returns>True if file successfully processed</returns>
public static bool Analyse(FileDirData DirData, double[] background)
{
TifFileInfo fi = new TifFileInfo();
// try
{
//read celltool 3 image
var console = Console.Out;
Console.SetOut(TextWriter.Null);
Console.SetError(TextWriter.Null);
fi = Cell_Tool_3.FileDecoder.ReadImage(DirData.InputDir); //read the image
Cell_Tool_3.FileDecoder.LoadRoiSet(DirData.InputRoi, fi); //read roi file
Console.SetOut(console);
Console.SetError(console);
int curInd = 1;
if (fi.roiList != null && fi.roiList[0] != null)
{
foreach (ROI roi in fi.roiList[0])
{
if (roi.Checked && roi.Type == 1 && roi.Shape > 1)
{
FileDirData curDirData = DirData.Duplicate();
if (!curDirData.InputDir.EndsWith("_CompositeRegistred.tif") &&
!curDirData.InputDir.EndsWith("_Q2.tif") &&
!curDirData.InputDir.EndsWith("_1.tif"))
{
curDirData.ExtractClipNumber();
curDirData.AddCellIndex(curInd.ToString());
}
ProcessSingleRoi(fi, roi, curDirData, background);
}
curInd++;
}
}
//clear and report
fi.Delete();
return(true);
}
//catch
{
//clear and report
fi.Delete();
return(false);
}
}
